##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 798247 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22759997845279 33.0 31.0 34.0 31.0 34.0 2 32.460115728590274 34.0 31.0 34.0 31.0 34.0 3 32.53627761833117 34.0 31.0 34.0 31.0 34.0 4 36.13853982539239 37.0 35.0 37.0 35.0 37.0 5 35.99963920941764 37.0 35.0 37.0 35.0 37.0 6 36.080114300460885 37.0 35.0 37.0 35.0 37.0 7 36.04240040989819 37.0 35.0 37.0 35.0 37.0 8 36.024601407834915 37.0 35.0 37.0 35.0 37.0 9 37.734171252757605 39.0 37.0 39.0 35.0 39.0 10-11 37.65307793201853 39.0 37.0 39.0 35.0 39.0 12-13 37.574810804174646 39.0 37.0 39.0 35.0 39.0 14-15 38.81798741492295 40.0 38.0 41.0 35.0 41.0 16-17 38.80607005099925 40.0 38.0 41.0 35.0 41.0 18-19 38.806331248347945 40.0 38.0 41.0 35.0 41.0 20-21 38.71200580772618 40.0 38.0 41.0 34.0 41.0 22-23 38.58708707956309 40.0 38.0 41.0 34.0 41.0 24-25 38.44714668517389 40.0 38.0 41.0 34.0 41.0 26-27 38.2394127381625 40.0 38.0 41.0 34.0 41.0 28-29 38.21605718530731 40.0 38.0 41.0 34.0 41.0 30-31 38.36396315927276 40.0 38.0 41.0 34.0 41.0 32-33 38.33948264133783 40.0 38.0 41.0 34.0 41.0 34-35 38.2372573902564 40.0 38.0 41.0 34.0 41.0 36-37 38.09788261026976 40.0 38.0 41.0 34.0 41.0 38-39 37.95984325653588 40.0 37.5 41.0 33.0 41.0 40-41 37.80659056657902 40.0 37.0 41.0 33.0 41.0 42-43 37.61945488050691 40.0 37.0 41.0 33.0 41.0 44-45 37.338485456256024 40.0 37.0 41.0 32.5 41.0 46-47 37.00309929132211 40.0 37.0 41.0 31.5 41.0 48-49 36.693386257637044 40.0 36.0 41.0 31.0 41.0 50-51 36.479444958765896 39.5 36.0 41.0 30.0 41.0 52-53 36.25163702463022 39.0 36.0 41.0 30.0 41.0 54-55 36.053374456778414 39.0 35.0 41.0 29.5 41.0 56-57 35.807585872543214 39.0 35.0 40.0 29.0 41.0 58-59 35.561439629588335 38.5 35.0 40.0 28.0 41.0 60-61 35.242149359784634 38.0 35.0 40.0 28.0 41.0 62-63 34.88983234512626 38.0 34.0 40.0 27.5 41.0 64-65 34.5462870515016 37.0 34.0 40.0 26.5 41.0 66-67 34.16921391499122 37.0 34.0 39.5 26.0 41.0 68-69 33.745319431203626 36.0 33.0 39.0 26.0 40.5 70-71 33.35589610734522 36.0 33.0 39.0 25.5 40.0 72-73 32.92354308879332 35.0 33.0 38.0 24.5 40.0 74-75 32.49192668434708 35.0 32.0 37.0 23.5 39.5 76-77 31.154785423559375 34.0 30.5 36.0 21.5 38.5 78-79 31.771098419411537 35.0 32.0 36.0 23.0 39.0 80-81 31.601600757660222 35.0 32.0 36.0 22.5 37.5 82-83 31.27322871241608 35.0 32.0 35.5 20.5 37.0 84-85 30.95540227523561 34.0 31.0 35.0 20.0 37.0 86-87 30.62873646878723 34.0 31.0 35.0 18.5 36.0 88-89 30.300280489622885 34.0 31.0 35.0 17.5 36.0 90-91 29.889557993954252 34.0 30.5 35.0 12.0 35.5 92-93 29.514282546630305 34.0 30.0 35.0 6.0 35.0 94-95 29.150569936373078 34.0 30.0 35.0 2.0 35.0 96-97 28.776196465505038 34.0 29.0 35.0 2.0 35.0 98-99 28.20641480644462 34.0 29.0 35.0 2.0 35.0 100-101 26.68649365421981 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05088539927710656 1101 2 0.15986427979500917 1101 3 0.16205267690152425 1101 4 0.09720399731264706 1101 5 -0.07335475597583496 1101 6 -0.03584357780013647 1101 7 0.016731345633054673 1101 8 0.035581931954247636 1101 9 0.08063726188933629 1101 10-11 0.131621486397286 1101 12-13 0.07492181287673105 1101 14-15 0.07321911629410494 1101 16-17 0.14708329661975483 1101 18-19 0.23976256285730102 1101 20-21 0.04511437053530898 1101 22-23 0.11658496710566624 1101 24-25 0.1783256288698567 1101 26-27 0.16638515329842818 1101 28-29 0.0769861467064672 1101 30-31 0.09961210535553988 1101 32-33 0.14595092367274276 1101 34-35 0.0547616518094145 1101 36-37 0.03924188738092482 1101 38-39 0.02220632906118425 1101 40-41 0.11731177292234918 1101 42-43 0.0038793377474988233 1101 44-45 -0.028242180231025316 1101 46-47 -0.16560881825195395 1101 48-49 -0.015971541133986022 1101 50-51 -0.0759457516528883 1101 52-53 -0.16634049083388192 1101 54-55 -0.11027285632753347 1101 56-57 -0.19093110344671516 1101 58-59 -0.046696939261124726 1101 60-61 -0.037403895130545095 1101 62-63 -0.16564229270053232 1101 64-65 0.030907838509129704 1101 66-67 -0.03285755899580778 1101 68-69 -0.09092142513439683 1101 70-71 -0.07719380389726638 1101 72-73 -0.18027486835970308 1101 74-75 -0.021174129649928375 1101 76-77 -0.010943620635664786 1101 78-79 -0.013853519199724218 1101 80-81 -0.1511500939851267 1101 82-83 -0.16794828827262265 1101 84-85 -0.21643961666679346 1101 86-87 -0.2672049888876771 1101 88-89 -0.2887340548936237 1101 90-91 -0.04853169759812559 1101 92-93 0.14778353249854703 1101 94-95 -0.06584520639621161 1101 96-97 -0.20200346060848418 1101 98-99 -0.1534675639600458 1101 100-101 -0.10698264078183684 1102 1 0.21960129194933842 1102 2 0.22748924458581143 1102 3 0.058232656191421484 1102 4 0.06418891460396026 1102 5 -0.0570759477590741 1102 6 0.10016930218056785 1102 7 0.11616465485188598 1102 8 0.16112780118069736 1102 9 0.20259888538294035 1102 10-11 0.12146325271357483 1102 12-13 0.18160568392291054 1102 14-15 0.25018611995586326 1102 16-17 0.28387989789356993 1102 18-19 0.152240854740036 1102 20-21 0.1752802438551413 1102 22-23 0.042743679026536086 1102 24-25 0.03680060956812525 1102 26-27 0.20395514107907076 1102 28-29 0.028373389122045012 1102 30-31 0.11673492111872008 1102 32-33 0.14050565649825586 1102 34-35 0.21656843588736052 1102 36-37 0.2310489348946021 1102 38-39 0.190078720794169 1102 40-41 0.1628879271254604 1102 42-43 0.2955439779854814 1102 44-45 0.13383487877249678 1102 46-47 0.014360592807079797 1102 48-49 0.20857600800183462 1102 50-51 0.14941692842062793 1102 52-53 0.03227409053635455 1102 54-55 0.19127619641837867 1102 56-57 0.14942887134451155 1102 58-59 0.23038349374616018 1102 60-61 0.21851175680195212 1102 62-63 0.3344045684627659 1102 64-65 0.33404231839580945 1102 66-67 0.3734332862073231 1102 68-69 0.28641327401187056 1102 70-71 0.27636708724501347 1102 72-73 0.2298958278603891 1102 74-75 0.1739101596511361 1102 76-77 0.19360155757860653 1102 78-79 0.2246946257061353 1102 80-81 0.3514766641950615 1102 82-83 0.4478692034544842 1102 84-85 0.3856956818970758 1102 86-87 0.5822820206912525 1102 88-89 0.5371957212468033 1102 90-91 0.41153195252680064 1102 92-93 0.2819888380941258 1102 94-95 0.20294181392279143 1102 96-97 0.33381417296862637 1102 98-99 0.3432361964836588 1102 100-101 0.43744655478677075 1103 1 0.07747093553326323 1103 2 0.013755887995309024 1103 3 -0.0024394759092842833 1103 4 0.011860290846769317 1103 5 -0.11402693328148672 1103 6 -0.12862306946894364 1103 7 -0.08439933142638978 1103 8 0.021904590528919243 1103 9 0.009884192668245362 1103 10-11 -0.071309582916399 1103 12-13 -0.07916529113377635 1103 14-15 -0.11259659996971294 1103 16-17 -0.19829929857134232 1103 18-19 -0.15792252685191954 1103 20-21 0.025097093671995196 1103 22-23 -0.012244983104977791 1103 24-25 -0.10287320867712424 1103 26-27 -0.029038056199844675 1103 28-29 -0.14072074425779846 1103 30-31 -0.3135953306401049 1103 32-33 -0.13176308005619575 1103 34-35 -0.13509445148981314 1103 36-37 -0.22341062670781753 1103 38-39 -0.19119344619229395 1103 40-41 -0.0565579708918591 1103 42-43 -0.1372864191306249 1103 44-45 -0.15735937670746836 1103 46-47 -0.25540799839008343 1103 48-49 -0.32330084530358505 1103 50-51 -0.3280090847196604 1103 52-53 -0.23439839042183763 1103 54-55 -0.13989131332055393 1103 56-57 -0.3320090029505067 1103 58-59 -0.49239108617128124 1103 60-61 -0.5806044708856959 1103 62-63 -0.3424371083789097 1103 64-65 -0.2556576641613191 1103 66-67 -0.21491487356940553 1103 68-69 -0.4614354381225567 1103 70-71 -0.4736183770261633 1103 72-73 -0.4517862466788216 1103 74-75 -0.5023936952241428 1103 76-77 -0.5763705338220575 1103 78-79 -0.5884049731077425 1103 80-81 -0.5491134863950933 1103 82-83 -0.44676129831887224 1103 84-85 -0.5949717987704055 1103 86-87 -0.402266499571553 1103 88-89 -0.36288567883459777 1103 90-91 -0.25932100404043723 1103 92-93 -0.14781812742712575 1103 94-95 -0.339502514235825 1103 96-97 -0.16247921618190375 1103 98-99 -0.02130534662931538 1103 100-101 0.1627358157903167 1104 1 -0.05644128320038533 1104 2 0.03037022603822237 1104 3 -0.001965247756480437 1104 4 0.08001832329051695 1104 5 -0.1227592723709705 1104 6 -0.13820574869448166 1104 7 -0.049764323577093705 1104 8 0.08715184327616754 1104 9 -0.038944954375182306 1104 10-11 0.01085453272985859 1104 12-13 0.009327317448118322 1104 14-15 -0.08273657903826148 1104 16-17 -0.06918659579792319 1104 18-19 -0.05936483454422614 1104 20-21 -0.13259194348133008 1104 22-23 -0.2230393970243938 1104 24-25 -0.179550995176335 1104 26-27 -0.007288971950764278 1104 28-29 -0.19183109224314876 1104 30-31 -0.16947903027379851 1104 32-33 -0.13726576189816342 1104 34-35 -0.16597651585401962 1104 36-37 0.08734781128276126 1104 38-39 -0.07934918925874257 1104 40-41 -0.12338325869929179 1104 42-43 -0.1689043547664184 1104 44-45 0.13854263794454624 1104 46-47 0.04330667978888414 1104 48-49 -0.16315072756417948 1104 50-51 -0.15628125897398348 1104 52-53 -0.15664132605763115 1104 54-55 -0.10689157496367585 1104 56-57 -0.2704084011713306 1104 58-59 -0.23169642369090582 1104 60-61 -0.6004484661761254 1104 62-63 -0.4269805411366363 1104 64-65 -0.3069560681382981 1104 66-67 -0.4474093136531323 1104 68-69 -0.20134582535437318 1104 70-71 -0.1338420818927304 1104 72-73 -0.2223088788086045 1104 74-75 -0.15352530171289658 1104 76-77 -0.15784783628150123 1104 78-79 -0.1529081782359505 1104 80-81 -0.25470774332863755 1104 82-83 -0.23779838623095984 1104 84-85 -0.26852295000012916 1104 86-87 -0.13614274237636437 1104 88-89 -0.30054920578383815 1104 90-91 -0.1881867811204998 1104 92-93 -0.2066387710691977 1104 94-95 -0.385347294455606 1104 96-97 -0.5046184757318777 1104 98-99 -0.291926094928634 1104 100-101 -0.19212584041062897 1105 1 0.003909152034736962 1105 2 0.004942087141444063 1105 3 0.06953217394850242 1105 4 0.07585520473624996 1105 5 -0.047131256475424266 1105 6 0.061121292652856596 1105 7 0.12687845246605178 1105 8 0.05154653530001241 1105 9 0.07998185989296047 1105 10-11 0.11467378005695394 1105 12-13 -0.005599887112389013 1105 14-15 -0.007735396334133782 1105 16-17 -0.05122279837148369 1105 18-19 0.0016006068409595287 1105 20-21 0.1408995173948142 1105 22-23 0.0037012477858979764 1105 24-25 0.13194666306947767 1105 26-27 0.010563946963038973 1105 28-29 0.016450497784290974 1105 30-31 -0.12945336559408105 1105 32-33 0.08890127140678317 1105 34-35 0.10675357923539508 1105 36-37 0.12501545959385396 1105 38-39 0.16225213300447905 1105 40-41 0.3167172120169539 1105 42-43 0.2617256125909435 1105 44-45 0.097582636998375 1105 46-47 0.20344186287148602 1105 48-49 0.2809354560861195 1105 50-51 0.3707243115624905 1105 52-53 0.44640472844137236 1105 54-55 0.49244965883327296 1105 56-57 0.4653488842861364 1105 58-59 0.19693329529859227 1105 60-61 0.3028610356688901 1105 62-63 0.3330901845706222 1105 64-65 0.5132936007045501 1105 66-67 0.40805980594191027 1105 68-69 0.3496435066543242 1105 70-71 0.4622230614510201 1105 72-73 0.40084905950155303 1105 74-75 0.5123193855801347 1105 76-77 0.508220086416955 1105 78-79 0.5408527617773018 1105 80-81 0.3415155866931272 1105 82-83 0.38237174575882094 1105 84-85 0.41870798480922033 1105 86-87 0.48303243976006627 1105 88-89 0.43321226644626876 1105 90-91 0.25513212322238843 1105 92-93 0.3520203972532556 1105 94-95 0.7176322329358236 1105 96-97 0.7322461164717353 1105 98-99 0.7408791610085395 1105 100-101 0.6062925884852 1106 1 -0.16692313891528698 1106 2 -0.0368288178557421 1106 3 -0.12869004132449646 1106 4 -0.043379112606920955 1106 5 -0.017071527912037254 1106 6 -0.03949866481790565 1106 7 -0.08496640962663804 1106 8 -0.047545027649512406 1106 9 -0.10003252499665649 1106 10-11 -0.15424352246353834 1106 12-13 -0.2655735408743496 1106 14-15 -0.00440107273666257 1106 16-17 0.08000804783527826 1106 18-19 -0.08527748121958467 1106 20-21 -0.41870395999588794 1106 22-23 -0.040014353565034355 1106 24-25 -0.10226122985691433 1106 26-27 0.07893712539821962 1106 28-29 -0.04865285452041945 1106 30-31 -0.07097566217297668 1106 32-33 -0.19060534251528338 1106 34-35 -0.21822961233381477 1106 36-37 -0.15419798402362517 1106 38-39 -0.10909848304405756 1106 40-41 -0.2526273373607495 1106 42-43 -0.4420668384691595 1106 44-45 -0.19299799656219108 1106 46-47 -0.129851729598343 1106 48-49 -0.255658061332106 1106 50-51 -0.18034009209002022 1106 52-53 -0.27059993101200064 1106 54-55 -0.31074316123051915 1106 56-57 -0.2412091399805476 1106 58-59 -0.25117165185257306 1106 60-61 -0.2712641441422292 1106 62-63 -0.1406104846394598 1106 64-65 -0.07181373871630115 1106 66-67 -0.1490947221711636 1106 68-69 -0.007381244737253212 1106 70-71 -0.23064781852897198 1106 72-73 -0.1933133333935615 1106 74-75 -0.29391263330784767 1106 76-77 -0.22559691276998706 1106 78-79 -0.07634499918336246 1106 80-81 -0.0629636646671834 1106 82-83 -0.1533989403378726 1106 84-85 -0.059659955649845386 1106 86-87 0.14729380695001382 1106 88-89 -0.03825835398027522 1106 90-91 -0.11647882805661425 1106 92-93 -0.05776328215133475 1106 94-95 -0.40912092548559187 1106 96-97 -0.1695535583046741 1106 98-99 -0.09641327441581282 1106 100-101 -0.31677952359185113 1107 1 -0.14783457128484656 1107 2 -0.15892087501758567 1107 3 -0.12767226133114207 1107 4 0.005640495236214349 1107 5 -0.04621326935438219 1107 6 -0.07165220419975782 1107 7 -0.09576734016834365 1107 8 -0.06443186713106996 1107 9 -0.14556260143400834 1107 10-11 -0.0414880389141814 1107 12-13 -0.05305484695610829 1107 14-15 -0.15961252172303375 1107 16-17 -0.0809465882564453 1107 18-19 -0.0625228285110424 1107 20-21 -0.134618715728692 1107 22-23 -0.13374358254475993 1107 24-25 -0.19600084434677711 1107 26-27 -0.08628595535950723 1107 28-29 -0.15739953115717498 1107 30-31 -0.12573845712146436 1107 32-33 -0.2625250002088677 1107 34-35 -0.22246690046940643 1107 36-37 -0.017078093694614438 1107 38-39 0.04105522250595328 1107 40-41 -0.028902678005479743 1107 42-43 -0.029834784630672573 1107 44-45 -0.1315365475758199 1107 46-47 -0.13509641221715185 1107 48-49 -0.20677346512074024 1107 50-51 0.0816759431376326 1107 52-53 -0.16870769106517258 1107 54-55 -0.15586404856850322 1107 56-57 -0.22379263948204198 1107 58-59 -0.07389099382665165 1107 60-61 -0.03274013284278965 1107 62-63 -0.09609818819545524 1107 64-65 -0.01665629438263494 1107 66-67 -0.13568702858525938 1107 68-69 -0.19036354782797815 1107 70-71 -0.4662327304598435 1107 72-73 -0.05022177473620104 1107 74-75 -0.007220625996453123 1107 76-77 -0.03734915604017175 1107 78-79 0.08957391075349364 1107 80-81 0.2298557936246084 1107 82-83 0.2363484840556147 1107 84-85 0.2719182264704614 1107 86-87 0.11512988205802088 1107 88-89 -0.10329241845352044 1107 90-91 0.034993837280705264 1107 92-93 0.02611009770908268 1107 94-95 -0.22893232462151758 1107 96-97 -0.10758321932162218 1107 98-99 -0.14604562735699034 1107 100-101 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2316 90-91 -0.5396859539906416 2316 92-93 -0.4915488206969876 2316 94-95 -0.40491666997256104 2316 96-97 -0.30670956340764377 2316 98-99 -0.5663729518351666 2316 100-101 -0.8021677138597596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 12.0 6 28.0 7 80.0 8 186.0 9 351.0 10 613.0 11 972.0 12 1506.0 13 1997.0 14 2697.0 15 3834.0 16 5294.0 17 7651.0 18 6862.0 19 3982.0 20 3434.0 21 3598.0 22 3552.0 23 3949.0 24 4400.0 25 5239.0 26 6292.0 27 7828.0 28 9700.0 29 12411.0 30 15577.0 31 19558.0 32 25545.0 33 33883.0 34 47250.0 35 68781.0 36 111568.0 37 185240.0 38 175021.0 39 19346.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48599976937847 20.16910573996922 15.038403497425563 22.306490993226745 2 15.540052139250133 21.16036765562539 39.58787192435424 23.71170828077024 3 19.29528078401798 25.17503980597484 30.103589490470995 25.42608991953618 4 13.064017859156198 17.58029479634098 37.33698634255605 32.018701001946766 5 13.600225507391631 37.94788273615635 34.02718616887998 14.424705587572037 6 31.553516643344732 37.094032298273596 17.197934975013997 14.15451608336768 7 28.08842375856095 33.57782741432163 20.715016780520315 17.618732046597106 8 26.416886001450678 35.501668030070896 19.58579236752534 18.495653600953087 9 26.494305647249533 17.00162982134602 20.37001078613512 36.13405374526933 10-11 24.636860520615798 27.37962059362578 27.30357896741234 20.679939918346076 12-13 25.715436490486947 24.628579374833222 28.0531408047165 21.60284332996333 14-15 22.46716868337745 26.087727232297773 26.340719100729476 25.104384983595303 16-17 21.652571196634625 29.676403418991864 26.843508337644867 21.827517046728644 18-19 21.707879891812933 27.832237390181234 29.560900322832406 20.898982395173423 20-21 22.302808529189587 26.802418299097898 29.76353183914252 21.131241332569996 22-23 22.025977247358867 27.11644536647637 29.56878721482467 21.288790171340093 24-25 22.10835415509177 27.171909323408205 29.52031554281369 21.19942097868633 26-27 21.993835114042874 27.387327344670492 29.45696763027722 21.161869911009415 28-29 21.644823423957266 27.574726690640677 29.621767282553613 21.158682602848444 30-31 21.865089531287875 27.22606783294007 29.63110741687499 21.27773521889706 32-33 21.57521418808965 27.426598534037712 29.52338060775675 21.474806670115893 34-35 21.978660740347287 27.253657076067935 29.614705723917535 21.152976459667247 36-37 22.033926969698488 27.173834065774376 29.373913239560835 21.4183257249663 38-39 21.950257033098296 27.331635021143295 29.474151684291822 21.243956261466582 40-41 21.883904282699643 27.42198055362598 29.440028863264665 21.25408630040971 42-43 21.819395965250337 27.567949524284423 29.11336739475247 21.49928711571277 44-45 21.9171509570346 27.71823758811496 28.762463247591285 21.602148207259155 46-47 22.06265729780381 27.887107624582363 28.40455397890628 21.64568109870754 48-49 22.249392888036464 28.09100948140706 28.090758932234593 21.568838698321883 50-51 22.23121414800181 28.11798854239352 28.02071288711389 21.630084422490782 52-53 22.356530666317568 28.45841285669634 27.63216328811124 21.55289318887485 54-55 21.990060720553913 28.296943176736022 27.82215279230614 21.890843310403923 56-57 22.042362827545862 28.53891088848439 27.814009949301404 21.604716334668343 58-59 22.167512060803237 28.166532414152513 27.929137221937573 21.736818303106684 60-61 22.140703316141494 28.02478430861626 28.415202312066317 21.419310063175935 62-63 21.96437944646206 28.24858721673868 28.290491539586114 21.49654179721314 64-65 21.925669623562634 28.23148724642874 28.149495081096454 21.693348048912178 66-67 21.562123002028194 28.43411876273885 28.043074386749968 21.96068384848299 68-69 21.74088972460905 28.164465384774385 28.145361022340204 21.94928386827636 70-71 21.71163812704589 28.335402450619924 28.10283032695394 21.850129095380254 72-73 21.759931449789352 28.123751169750715 27.972356927116543 22.14396045334339 74-75 21.77934899849295 28.356887028701642 27.764338606972526 22.099425365832882 76-77 21.693034862642765 28.202110374357815 27.93201853561617 22.172836227383254 78-79 21.73067985222619 28.11185009151303 27.648647599051422 22.50882245720936 80-81 21.673241490415872 28.31780138227892 27.76396278344923 22.244994343855975 82-83 21.506532781037365 28.442636864565763 27.860546311996277 22.190284042400595 84-85 21.38695339531924 28.36938738183467 27.662838272913366 22.58082094993273 86-87 21.323976162766662 28.113666571875623 27.64088057956998 22.92147668578773 88-89 21.440606729496007 27.995532711053094 27.626661922938638 22.937198636512257 90-91 21.315743316044 27.72131599388609 27.936868876694483 23.026071813375427 92-93 21.277580639604622 27.6824589481069 27.996592450147357 23.04336796214112 94-95 20.874428591651455 27.886105428520246 28.071762249028183 23.167703730800117 96-97 20.854008846885737 27.945297633439274 28.090991885970134 23.109701633704855 98-99 20.664066341490305 28.379290019774917 27.534424450990848 23.422219187743938 100-101 20.279999774476646 29.08035960951884 27.361433125747435 23.278207490257078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 101.0 2 71.5 3 100.0 4 107.0 5 98.0 6 163.5 7 331.5 8 553.5 9 731.5 10 800.5 11 838.5 12 957.5 13 1084.5 14 1166.0 15 1211.5 16 1271.0 17 1380.0 18 1434.0 19 1516.0 20 1671.5 21 1884.5 22 2219.0 23 2668.0 24 3142.5 25 3781.0 26 4810.5 27 6136.0 28 7265.5 29 8436.0 30 10283.0 31 11968.5 32 13432.0 33 15104.0 34 17182.5 35 18617.5 36 20255.5 37 22359.5 38 23263.5 39 23880.5 40 24798.0 41 26500.5 42 27922.5 43 29307.5 44 30795.0 45 31515.0 46 31467.0 47 31915.5 48 33927.0 49 33050.0 50 30694.5 51 29159.5 52 26672.5 53 25122.0 54 23829.5 55 21538.5 56 18958.5 57 16474.5 58 14613.5 59 12810.5 60 10716.0 61 8659.5 62 6741.5 63 5081.5 64 3644.5 65 2678.0 66 1956.5 67 1600.0 68 1201.0 69 693.0 70 491.0 71 395.0 72 302.0 73 218.5 74 155.0 75 110.0 76 73.5 77 39.0 78 24.0 79 15.5 80 10.0 81 5.5 82 5.0 83 5.0 84 2.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05048562662935157 2 0.0 3 0.0 4 1.2527450776514038E-4 5 0.005887901864961597 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035076862174239303 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012527450776514035 24-25 4.384607771779913E-4 26-27 0.002067029378124816 28-29 0.002693401916950518 30-31 8.142843004734123E-4 32-33 0.0 34-35 0.0 36-37 3.7582352329542106E-4 38-39 4.384607771779913E-4 40-41 6.263725388257019E-5 42-43 0.01014723512897637 44-45 0.0 46-47 0.0 48-49 6.263725388257019E-5 50-51 0.0 52-53 1.8791176164771053E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.384607771779913E-4 84-85 1.2527450776514038E-4 86-87 0.0 88-89 0.0 90-91 0.008393392020264404 92-93 0.002442852901420237 94-95 0.0 96-97 0.0 98-99 0.0016912058548293949 100-101 0.012965911553692026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 798247.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.00610267196407 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61257582699005 53.70321586204733 2 10.38618758640475 13.50331153224212 3 3.03077652533843 5.910569099457855 4 1.247547691214572 3.243928532130648 5 0.7149795875765461 2.3239018239179745 6 0.4245300663751856 1.6558227049272622 7 0.29442253959148607 1.3397483286337186 8 0.2033821886357865 1.0576866748885363 9 0.15831820459114787 0.9262484516243854 >10 0.8430876777799705 9.857587149827387 >50 0.057931263102570525 2.5581425186043347 >100 0.025295370258598498 2.7126810704919913 >500 3.861888563528816E-4 0.20623455555082185 >1k 5.792832845293223E-4 1.0009216956556624 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3366 0.42167399313746246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2794 0.3500169746958022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1814 0.2272479570859646 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 908 0.11374925305074744 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.263725388257018E-5 0.0 16-17 0.0 0.0 0.0 3.131862694128509E-4 0.0 18-19 0.0 0.0 0.0 3.758235232954211E-4 0.0 20-21 0.0 0.0 0.0 3.758235232954211E-4 0.0 22-23 0.0 0.0 0.0 5.637352849431317E-4 0.0 24-25 0.0 0.0 0.0 8.769215543559826E-4 0.0 26-27 0.0 0.0 0.0 0.0012527450776514035 0.0 28-29 0.0 0.0 0.0 0.0035703234713065 0.0 30-31 0.0 0.0 0.0 0.007328558704260711 0.0 32-33 0.0 0.0 0.0 0.013091186061457167 0.0 34-35 0.0 0.0 0.0 0.018227440879827923 0.0 36-37 0.0 0.0 0.0 0.025180176060793213 0.0 38-39 0.0 0.0 0.0 0.03520213668200445 0.0 40-41 0.0 0.0 0.0 0.04491091103380282 0.0 42-43 0.0 0.0 0.0 0.06771087144705837 0.0 44-45 0.0 0.0 0.0 0.09195148869961303 0.0 46-47 0.0 0.0 0.0 0.11850968434582279 0.0 48-49 0.0 0.0 0.0 0.14951512501769504 0.0 50-51 0.0 0.0 0.0 0.1882249479171234 0.0 52-53 0.0 0.0 0.0 0.2712819465654115 0.0 54-55 0.0 0.0 0.0 0.3333554651630385 0.0 56-57 0.0 0.0 0.0 0.45004866914626673 0.0 58-59 0.0 0.0 0.0 0.6082077351997565 0.0 60-61 0.0 0.0 0.0 0.7566580269014478 0.0 62-63 0.0 0.0 0.0 0.980335660516106 0.0 64-65 0.0 0.0 0.0 1.1198914621664722 0.0 66-67 0.0 0.0 0.0 1.4079601927724126 0.0 68-69 0.0 0.0 0.0 1.7176387759678395 0.0 70-71 0.0 0.0 0.0 1.9485823310328758 0.0 72-73 0.0 0.0 0.0 2.397816715878669 0.0 74-75 0.0 0.0 0.0 2.824188503057324 0.0 76-77 0.0 0.0 0.0 3.1172055767199875 0.0 78-79 0.0 0.0 0.0 3.45845333587223 0.0 80-81 0.0 0.0 0.0 4.015862258173222 0.0 82-83 0.0 0.0 0.0 4.52272291659098 0.0 84-85 0.0 0.0 0.0 5.180038258834671 0.0 86-87 0.0 0.0 0.0 6.057210362206185 0.0 88-89 0.0 0.0 0.0 7.308953243795466 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3205 0.0 33.506912 1 GTATCAA 4610 0.0 28.757908 1 CGTAGCA 90 8.9422136E-4 26.390368 7 TATCAAC 5475 0.0 24.203804 2 ATCAACG 5570 0.0 23.535175 3 TCAACGC 5605 0.0 23.38821 4 CAACGCA 5670 0.0 23.203861 5 TACGTAG 125 2.2561214E-4 22.798422 5 AACGCAG 5800 0.0 22.683773 6 GTGTAGC 380 0.0 22.50831 1 GTACATG 3380 0.0 21.931175 1 ACGTAGC 110 0.0028777604 21.589415 6 GAGTACT 3315 0.0 21.566065 12-13 GTACTTT 3390 0.0 20.736027 14-15 GGGCGTA 115 0.003721087 20.653332 9 TTACGTA 140 4.8522168E-4 20.355734 4 CGCAGAG 6475 0.0 20.174877 8 TACATGG 3595 0.0 20.08206 2 ACGCAGA 6535 0.0 19.989643 7 ACATGGG 3455 0.0 19.933498 3 >>END_MODULE