FastQCFastQC Report
Thu 26 May 2016
SRR937401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.4627980779753082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.36311543091355863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.224733632447597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12030.11967886668192082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.039.528681
GTATCAA50300.035.0637251
TATCAAC61600.028.3858992
ATCAACG61650.028.2858053
TCAACGC61450.028.2246234
CAACGCA62000.027.9770365
AACGCAG63550.027.3571746
ACGCAGA71150.024.3682127
CGCAGAG71400.024.2828888
GTACATG40250.023.9763031
GAGTACT40500.022.342112-13
GCAGAGT76600.022.1383449
GGGCGCG2154.418871E-822.0936377
TTAGGGC3101.0913936E-1121.4586283
TACATGG42700.021.3652952
AGACGGC1353.7947687E-421.1116986
GTGTAGC5000.020.917261
GTATAGC4550.020.8963621
ACATGGG41900.020.8660093
GGACCGT1150.003722354320.6527486
Produced by FastQC (version 0.11.5)