##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937401_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1005190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67772361444105 33.0 31.0 34.0 30.0 34.0 2 31.77122733015649 33.0 31.0 34.0 30.0 34.0 3 31.814881763646675 33.0 31.0 34.0 30.0 34.0 4 35.4409096787672 37.0 35.0 37.0 33.0 37.0 5 35.42886618450243 37.0 35.0 37.0 33.0 37.0 6 35.48171589450751 37.0 35.0 37.0 33.0 37.0 7 35.42410390075508 37.0 35.0 37.0 33.0 37.0 8 35.4173788040072 37.0 35.0 37.0 33.0 37.0 9 37.034999353356085 39.0 37.0 39.0 33.0 39.0 10-11 36.94059531033934 39.0 37.0 39.0 33.0 39.0 12-13 36.82968195067599 39.0 37.0 39.0 33.0 39.0 14-15 37.986661725643906 40.0 38.0 41.0 33.0 41.0 16-17 37.930833474268546 40.0 38.0 41.0 33.0 41.0 18-19 37.89400959022672 40.0 38.0 41.0 33.0 41.0 20-21 37.83122295287458 40.0 38.0 41.0 33.0 41.0 22-23 37.69656681821347 40.0 37.5 41.0 32.0 41.0 24-25 37.5465568698455 40.0 37.0 41.0 32.0 41.0 26-27 37.3880341030054 40.0 37.0 41.0 32.0 41.0 28-29 37.19628229488953 39.0 37.0 41.0 31.0 41.0 30-31 37.19119569434635 39.0 37.0 41.0 31.0 41.0 32-33 37.03750087048219 39.0 36.0 41.0 31.0 41.0 34-35 36.838737950039295 39.0 36.0 41.0 30.5 41.0 36-37 36.861363523314 39.0 36.0 41.0 31.0 41.0 38-39 36.84529243227648 39.5 36.0 41.0 31.0 41.0 40-41 36.68579820730409 39.5 36.0 41.0 30.0 41.0 42-43 36.45254379768999 39.0 36.0 41.0 30.0 41.0 44-45 36.22315631870592 39.0 35.0 41.0 30.0 41.0 46-47 35.878053402839264 39.0 35.0 41.0 28.5 41.0 48-49 35.56409882708742 39.0 35.0 41.0 27.0 41.0 50-51 34.631769118276154 38.0 34.0 39.5 25.0 40.5 52-53 34.76405604910514 38.0 34.5 39.5 26.0 40.5 54-55 35.12913429301923 39.0 35.0 40.0 26.0 41.0 56-57 34.914671355664105 38.5 34.5 40.0 25.5 41.0 58-59 34.68067181328903 38.0 34.0 40.0 25.0 41.0 60-61 34.41862682676907 38.0 34.0 40.0 23.5 41.0 62-63 34.10887742615824 38.0 34.0 40.0 23.0 41.0 64-65 33.76239715874611 37.0 33.5 40.0 22.0 41.0 66-67 33.394836797023444 37.0 33.0 39.5 21.5 41.0 68-69 32.91064525114655 36.0 32.5 39.0 19.5 41.0 70-71 32.487193465911915 36.0 32.0 39.0 18.5 40.0 72-73 32.080207721923216 35.0 32.0 38.5 17.5 40.0 74-75 31.626252748236652 35.0 31.0 37.5 13.5 39.5 76-77 31.19318785503238 35.0 31.0 37.0 9.5 39.0 78-79 30.723825346451914 35.0 31.0 36.5 7.0 39.0 80-81 30.27786687093982 34.0 30.0 36.0 4.5 38.5 82-83 29.843931992956556 34.0 30.0 35.5 2.0 37.0 84-85 29.401998129706822 34.0 29.0 35.0 2.0 37.0 86-87 28.988076383569275 34.0 29.0 35.0 2.0 36.0 88-89 28.614536555278107 34.0 29.0 35.0 2.0 36.0 90-91 28.19795461554532 34.0 28.5 35.0 2.0 35.5 92-93 27.775432007879107 34.0 27.0 35.0 2.0 35.0 94-95 27.26798366477979 33.5 26.5 35.0 2.0 35.0 96-97 26.681200071628247 33.0 25.0 35.0 2.0 35.0 98-99 25.998129706821594 33.0 24.5 35.0 2.0 35.0 100-101 24.205581034431304 30.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03999097823341202 1101 2 0.04511084276784061 1101 3 0.11224896703675924 1101 4 -0.011078005294116622 1101 5 0.0707362763178665 1101 6 -0.1539843479603391 1101 7 0.05832928565922657 1101 8 -0.046548770865790345 1101 9 -0.00898806606522129 1101 10-11 -0.08948597942818992 1101 12-13 0.10381051822935916 1101 14-15 0.05028976765078852 1101 16-17 0.05710333954185387 1101 18-19 0.14192891993232593 1101 20-21 0.065388335825034 1101 22-23 0.09003764821851945 1101 24-25 0.15271105295626342 1101 26-27 0.16367581475061144 1101 28-29 0.011410836023777904 1101 30-31 0.015493344370597129 1101 32-33 -0.08472368258498619 1101 34-35 -0.17022610936282945 1101 36-37 -0.03235323671390944 1101 38-39 0.10349828243343495 1101 40-41 0.012814423899762062 1101 42-43 0.20684313807495158 1101 44-45 0.10821033751910392 1101 46-47 0.13779209498887468 1101 48-49 0.218156396693864 1101 50-51 0.10091539261756566 1101 52-53 0.19851856442310378 1101 54-55 0.3882552228627105 1101 56-57 0.41401127543964833 1101 58-59 0.35626489585123267 1101 60-61 0.44472214425831424 1101 62-63 0.5438681147153019 1101 64-65 0.38272726050814043 1101 66-67 0.5794205289820553 1101 68-69 0.3202641760005065 1101 70-71 0.3524870919836829 1101 72-73 0.32882728201349565 1101 74-75 0.2270179348846426 1101 76-77 0.3945413321961553 1101 78-79 0.609203198419241 1101 80-81 0.3883432132885716 1101 82-83 0.40879649512750404 1101 84-85 0.46430269193713514 1101 86-87 0.5926604636336741 1101 88-89 0.6999064236560244 1101 90-91 0.9435834658530986 1101 92-93 0.6308313054283694 1101 94-95 0.7629472282277234 1101 96-97 0.7584585616824526 1101 98-99 0.869623280283303 1101 100-101 1.090880492064386 1102 1 0.05361500308202238 1102 2 -0.014390660202877825 1102 3 -0.09797823534579564 1102 4 0.049000266159346495 1102 5 -0.07655955041421691 1102 6 0.039111421649899114 1102 7 -0.009837405982239034 1102 8 -8.436041557899898E-4 1102 9 0.0513553225072485 1102 10-11 -0.01828516964695126 1102 12-13 0.176967260507773 1102 14-15 0.3071393736679653 1102 16-17 0.22091028475652763 1102 18-19 0.2111140284617079 1102 20-21 0.16553429966237587 1102 22-23 0.2142465126932862 1102 24-25 0.31942681538571094 1102 26-27 0.35301383736967296 1102 28-29 0.2792781715881958 1102 30-31 0.257798437394257 1102 32-33 0.19823355585948121 1102 34-35 0.2524965196426905 1102 36-37 0.2503948110891372 1102 38-39 0.4034014570508404 1102 40-41 0.4231215883377004 1102 42-43 0.6915986902720732 1102 44-45 0.6756901154013306 1102 46-47 0.681492873818442 1102 48-49 0.5766758563330896 1102 50-51 0.6729149691132434 1102 52-53 0.6997207702316857 1102 54-55 0.59141434308372 1102 56-57 0.5588659754624032 1102 58-59 0.5970495144475976 1102 60-61 0.6285358262654839 1102 62-63 0.6210705613065883 1102 64-65 0.6578409171989748 1102 66-67 0.7678152725762288 1102 68-69 0.762837502656815 1102 70-71 0.6369603221988385 1102 72-73 0.6565380832093872 1102 74-75 0.5939829330211417 1102 76-77 0.5848608431511266 1102 78-79 0.6587591806098594 1102 80-81 0.7010697742243117 1102 82-83 0.8027462321663279 1102 84-85 0.5920204092317825 1102 86-87 0.5567663380057297 1102 88-89 0.5699533549360432 1102 90-91 0.520363475424638 1102 92-93 0.5688846200113922 1102 94-95 0.47254920674427225 1102 96-97 0.5424143652498756 1102 98-99 0.7513915047371782 1102 100-101 1.2591690265814819 1103 1 0.04759281696672346 1103 2 0.008010132278648996 1103 3 0.01648118505386975 1103 4 -4.694952262624952E-4 1103 5 -0.07141571670064195 1103 6 -0.053713309161750544 1103 7 0.009103295408813494 1103 8 0.16588199350267985 1103 9 0.027136087992772673 1103 10-11 -0.051672382744222034 1103 12-13 0.14301038115635123 1103 14-15 0.08894386869952342 1103 16-17 0.07746032418416604 1103 18-19 -0.017531204161358005 1103 20-21 6.264424774258259E-4 1103 22-23 -0.03114634655424453 1103 24-25 0.053556839603544404 1103 26-27 -0.15126918292784097 1103 28-29 -0.2024798802093457 1103 30-31 -0.029721662283307637 1103 32-33 0.0038644121267878973 1103 34-35 -0.135138214845135 1103 36-37 0.02195373225911368 1103 38-39 0.06305042477629996 1103 40-41 0.09640676663200765 1103 42-43 0.04960867486582998 1103 44-45 -0.11154304647087798 1103 46-47 -0.29625927365496096 1103 48-49 -0.07996342145357005 1103 50-51 -0.2294181369148376 1103 52-53 -0.16259047520467362 1103 54-55 -0.13759289420332266 1103 56-57 -0.022573147419940653 1103 58-59 -0.14301289627896807 1103 60-61 -0.18950842249065403 1103 62-63 -0.08864659799510832 1103 64-65 -0.08346746306867914 1103 66-67 -0.06812716026348653 1103 68-69 0.05969605985776383 1103 70-71 0.09617165715417997 1103 72-73 0.18727831259124983 1103 74-75 0.2547144069413676 1103 76-77 0.1989084481675576 1103 78-79 0.22580581186180737 1103 80-81 0.1754789910314294 1103 82-83 0.1399107815266447 1103 84-85 0.08757252246908465 1103 86-87 0.217024259313213 1103 88-89 0.3607665745606248 1103 90-91 0.2714087989698122 1103 92-93 0.21021446799749555 1103 94-95 0.26591329644285366 1103 96-97 0.4085091206722282 1103 98-99 0.3491355702459913 1103 100-101 0.8813119258179043 1104 1 -0.08995988753642692 1104 2 -0.09244107526068746 1104 3 -0.08178495642270533 1104 4 -0.031567437156411415 1104 5 0.017774120591511178 1104 6 -0.22832718583670442 1104 7 -0.013513536266906101 1104 8 -0.016285523210598285 1104 9 0.03850929232978473 1104 10-11 0.0685320585746041 1104 12-13 0.18278137935087813 1104 14-15 -0.018823600269200824 1104 16-17 0.08322811360057614 1104 18-19 0.011372531419837628 1104 20-21 -0.05083148693591255 1104 22-23 -0.042233513699969194 1104 24-25 0.1019213198062161 1104 26-27 -0.044313586438143204 1104 28-29 0.05155071716750825 1104 30-31 0.03116285934099494 1104 32-33 -0.03185501979904615 1104 34-35 0.008750052145856557 1104 36-37 -0.1597608026239783 1104 38-39 -0.08077654853084226 1104 40-41 -0.11460050365915464 1104 42-43 -0.17245404040135526 1104 44-45 0.15466160561219766 1104 46-47 -0.08183729997683287 1104 48-49 0.014603861268390972 1104 50-51 0.0578185825181734 1104 52-53 0.11474005582444846 1104 54-55 0.12755175351392012 1104 56-57 0.10859560484497166 1104 58-59 0.058324488889468284 1104 60-61 -0.03405446748882213 1104 62-63 0.2169196212276674 1104 64-65 0.2631848802318686 1104 66-67 0.20805061937819147 1104 68-69 0.38324848047437143 1104 70-71 0.20552281512090786 1104 72-73 0.3199896225663146 1104 74-75 0.4771594012367544 1104 76-77 0.37790670506121415 1104 78-79 0.31654035751968834 1104 80-81 0.5610536300707736 1104 82-83 0.6839204530247578 1104 84-85 0.6667220242159537 1104 86-87 0.6571750095156439 1104 88-89 0.5802818383008947 1104 90-91 0.6627068701543628 1104 92-93 0.7173162015334213 1104 94-95 0.8115260128391277 1104 96-97 0.5447692811756539 1104 98-99 0.39718014043210204 1104 100-101 0.7149802237568252 1105 1 -0.10828644676935184 1105 2 -0.09809038944251469 1105 3 -0.01820121934029828 1105 4 0.07214530925743645 1105 5 0.13489228818941967 1105 6 0.14328378090832672 1105 7 0.12287036326699763 1105 8 0.014892789514874494 1105 9 0.022637749579978106 1105 10-11 0.14492868136032655 1105 12-13 -0.04341884211619629 1105 14-15 0.08930976225462928 1105 16-17 0.08825633637488295 1105 18-19 0.16187931979820291 1105 20-21 0.10241587719340828 1105 22-23 0.050094936569884396 1105 24-25 -0.18128010100978287 1105 26-27 -0.04751898921949049 1105 28-29 -0.12469091039340441 1105 30-31 -0.2207353165698578 1105 32-33 -0.21873016059563355 1105 34-35 0.13631175674431262 1105 36-37 0.2624361951962513 1105 38-39 0.03666089019289842 1105 40-41 0.22412514051496402 1105 42-43 0.10392449696407624 1105 44-45 0.21711976324725413 1105 46-47 0.1931233563018182 1105 48-49 0.29993309523978695 1105 50-51 0.12872493176984534 1105 52-53 0.12919887341870862 1105 54-55 0.3490993419659105 1105 56-57 0.5057427091153457 1105 58-59 0.6597166696421439 1105 60-61 0.4121045223928661 1105 62-63 0.5045959937365581 1105 64-65 0.5053736444265411 1105 66-67 0.5420046475089464 1105 68-69 0.5555743765752226 1105 70-71 0.40128598812857774 1105 72-73 0.3415390354247343 1105 74-75 0.36808329651899996 1105 76-77 0.3747762615876731 1105 78-79 0.5366323869259446 1105 80-81 0.7456272424906984 1105 82-83 0.6037239089264759 1105 84-85 0.6731098263931834 1105 86-87 0.45294381438664644 1105 88-89 0.5719581822538586 1105 90-91 0.6054201115376152 1105 92-93 0.7154295273152975 1105 94-95 0.5234399421029288 1105 96-97 0.44056354841470835 1105 98-99 0.5845105896168263 1105 100-101 0.9349842057221665 1106 1 0.05893626231028293 1106 2 0.17427558383670316 1106 3 -0.0014597303765881975 1106 4 0.11960237612089486 1106 5 0.1525126829026604 1106 6 0.16718079262877694 1106 7 0.15408176700503873 1106 8 0.02618531907224053 1106 9 0.11570823115882689 1106 10-11 0.04107430809956725 1106 12-13 -0.139108104091612 1106 14-15 -0.2364567819289647 1106 16-17 -0.3642327862055623 1106 18-19 -0.08299266232643276 1106 20-21 0.02198767328727058 1106 22-23 0.19112856048021598 1106 24-25 -0.07991318476463505 1106 26-27 -0.05387247844969778 1106 28-29 0.2386161838878067 1106 30-31 0.23735907227034403 1106 32-33 0.3963930399537432 1106 34-35 0.341333819072311 1106 36-37 -0.0686640000591936 1106 38-39 -0.06985902740007788 1106 40-41 0.3035648957538015 1106 42-43 0.1235269138267725 1106 44-45 -0.008628331602814399 1106 46-47 0.3236145954308043 1106 48-49 0.06934461274871495 1106 50-51 -0.0333646346195664 1106 52-53 0.2771087176755813 1106 54-55 0.497518464700093 1106 56-57 0.3868534102412795 1106 58-59 0.1916256888038248 1106 60-61 0.21649738557568554 1106 62-63 0.3208854740290832 1106 64-65 0.2215402572826477 1106 66-67 0.19898092986575477 1106 68-69 0.06486886893669208 1106 70-71 0.2067038142846016 1106 72-73 0.28277662696674 1106 74-75 0.18267197887784548 1106 76-77 0.22885932971183465 1106 78-79 0.22754006851094744 1106 80-81 0.1936270272484748 1106 82-83 0.17634189497673347 1106 84-85 0.24138901605745744 1106 86-87 0.05084955879163289 1106 88-89 0.4210817107913911 1106 90-91 0.3932700723327791 1106 92-93 0.2076929786731192 1106 94-95 0.008350565331660675 1106 96-97 0.2693300311005231 1106 98-99 0.3603800144588547 1106 100-101 0.25752403834571425 1107 1 0.03632449156508244 1107 2 -0.14955584981382586 1107 3 -0.10208429665000907 1107 4 -0.21580587347745706 1107 5 0.1033668271898307 1107 6 -0.17303576170468205 1107 7 -0.3985994950561249 1107 8 -0.0371192734386625 1107 9 -0.2754318637144024 1107 10-11 -0.022407102260444844 1107 12-13 0.11663990195442153 1107 14-15 -0.16704056602105766 1107 16-17 -0.18754707517916813 1107 18-19 -0.058911212039816974 1107 20-21 -0.04592939343770297 1107 22-23 0.0863544785546182 1107 24-25 -0.1853222899332252 1107 26-27 -0.20690547494185552 1107 28-29 -0.04413456948931582 1107 30-31 -0.058673933728158545 1107 32-33 -0.24345371791036996 1107 34-35 0.09183432766128163 1107 36-37 0.18590712159338096 1107 38-39 0.13786011740993587 1107 40-41 0.11528525737482198 1107 42-43 0.10966382407745101 1107 44-45 0.08318956502528607 1107 46-47 0.019024936423072347 1107 48-49 -0.08302484149253786 1107 50-51 0.035454583389231686 1107 52-53 0.08145596511599962 1107 54-55 0.1889000205070417 1107 56-57 0.08050317781135874 1107 58-59 0.06067400950957591 1107 60-61 -0.3187007739976693 1107 62-63 -0.04804591874420083 1107 64-65 0.1979968656121045 1107 66-67 0.023986396008936595 1107 68-69 0.2949431134206364 1107 70-71 0.24937680583347088 1107 72-73 0.041059972816750445 1107 74-75 0.09042724776456268 1107 76-77 0.025037782650716878 1107 78-79 0.2739623589675375 1107 80-81 0.12159086514643391 1107 82-83 0.19523193518853432 1107 84-85 0.17566220914065056 1107 86-87 0.17490058880313697 1107 88-89 0.3275365129170069 1107 90-91 0.203292602349034 1107 92-93 0.2871279220628864 1107 94-95 0.37896172767135994 1107 96-97 0.5836941047556152 1107 98-99 0.5917271573295793 1107 100-101 1.1643743040478043 1108 1 -0.18466384411434333 1108 2 -0.1494839167363935 1108 3 -0.2428746923439462 1108 4 -0.21258004702220745 1108 5 -0.14646667254042 1108 6 -0.09175882555769022 1108 7 -0.03972537174050217 1108 8 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-1.2166905766374114 2316 54-55 -1.4839183348017713 2316 56-57 -1.2626121356283804 2316 58-59 -1.2304114635415502 2316 60-61 -1.8640598996048894 2316 62-63 -1.6694243018115174 2316 64-65 -1.471408061521835 2316 66-67 -1.7838698380877602 2316 68-69 -1.401870441980705 2316 70-71 -1.1039281906967133 2316 72-73 -1.3343751995172788 2316 74-75 -1.481231611823688 2316 76-77 -1.4163643108029014 2316 78-79 -1.2380706620826594 2316 80-81 -1.4768404903936982 2316 82-83 -1.476055365297242 2316 84-85 -1.7690665671721 2316 86-87 -1.6876051942179124 2316 88-89 -1.6701387126208118 2316 90-91 -1.4213054776879765 2316 92-93 -1.3527055269308654 2316 94-95 -1.3649111615259564 2316 96-97 -1.6583889394266649 2316 98-99 -1.991983518127281 2316 100-101 -2.2169985851495113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3819.0 3 753.0 4 750.0 5 809.0 6 864.0 7 1061.0 8 1345.0 9 1594.0 10 2127.0 11 2577.0 12 3460.0 13 4379.0 14 5116.0 15 6343.0 16 8069.0 17 10824.0 18 9287.0 19 6158.0 20 6109.0 21 6426.0 22 6829.0 23 7649.0 24 8780.0 25 9923.0 26 11238.0 27 13257.0 28 15571.0 29 18786.0 30 23041.0 31 28350.0 32 36147.0 33 47560.0 34 64212.0 35 91253.0 36 136493.0 37 199733.0 38 177631.0 39 26861.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20222108313704 18.97883229585721 15.159351146941324 22.659595474064425 2 14.964799440061338 19.979819874432557 41.240838432371156 23.81454225313495 3 18.786966319022074 24.488603505693263 30.72197738648374 26.002452788800923 4 12.382468896010273 16.48137549530075 38.2755058698249 32.86064973886408 5 13.40480515570196 36.82301003184428 35.05821500569289 14.713969806760877 6 31.15909460091066 36.246558114180424 18.129607243830517 14.464740041078402 7 27.936981883487444 32.64161436085924 21.470628686238904 17.95077506941441 8 26.02932896172685 34.511806825486204 20.471774736034362 18.987089476752587 9 26.090704434769002 16.600910651876465 21.0476383143473 36.260746599007234 10-11 24.33942932434466 26.50121544406316 28.281923222207496 20.877432009384684 12-13 25.413636125791427 23.92294639586775 28.822742324913847 21.84067515342697 14-15 22.36356279903174 25.210947862717216 27.226400597141655 25.199088741109392 16-17 21.49596607416045 28.735255864017578 27.783159667718127 21.985618394103838 18-19 21.386361892462062 26.963508610389468 30.585791487169367 21.064338009979103 20-21 21.998727363235147 25.960344957313847 30.673934415855243 21.366993263595763 22-23 21.749195769768917 25.980074622998394 30.693690928442447 21.57703867879025 24-25 21.697731047090603 26.116501516471917 30.685200160840196 21.500567275597284 26-27 21.500211774671747 26.359169843286743 30.53591449286195 21.60470388917956 28-29 21.2743821591879 26.486871365440777 30.674471548604377 21.56427492676695 30-31 21.35999685084412 26.209198624536405 30.731874789163516 21.69892973545596 32-33 21.201696378875887 26.392434361488554 30.61549293599015 21.79037632364541 34-35 21.754253620834323 26.31674402938521 30.397469651160243 21.531532698620225 36-37 21.643651735611808 26.160985929949703 30.44707462737137 21.748287707067114 38-39 21.546153922809395 26.392041774208014 30.447438439844944 21.61436586313765 40-41 21.668093358459053 26.224405498028375 30.517635006731524 21.589866136781044 42-43 21.409356949502328 26.416872981794608 30.198057540371583 21.97571252833148 44-45 21.559080793796483 26.563261449965168 29.96703310940164 21.910624646836705 46-47 21.689308430548593 26.717065475935424 29.57353499500264 22.020091098513348 48-49 21.803403497967608 26.935656010929776 29.29141791156326 21.96952257953935 50-51 21.756808672527477 27.049478072778317 29.232458795678284 21.96125445901592 52-53 21.94043230007778 27.15615630728694 28.960637164014223 21.942774228621058 54-55 21.691479665997445 26.93023907084424 29.09995011986679 22.27833114329152 56-57 21.812713869186297 27.254133890829806 28.95741449716701 21.975737742816882 58-59 21.986330068220123 26.97243245370742 28.949984032316 22.091253445756458 60-61 21.894037418780737 26.70025465019354 29.56009938880964 21.84560854221608 62-63 21.6927199331155 26.870787955608304 29.522899869559282 21.913592241716913 64-65 21.67526537241821 26.839620643869093 29.349130704479382 22.135983279233315 66-67 21.446295569192625 27.056904314411735 29.176142082405722 22.32065803398992 68-69 21.47482575354833 26.962922587192978 29.35441745708049 22.207834202178198 70-71 21.51356387815554 26.878377267393628 29.25896307147288 22.349095782977948 72-73 21.578139076913263 26.702334824240392 29.23284061645419 22.48668548239215 74-75 21.47301162762309 26.969339287733973 29.006328253026485 22.551320831616454 76-77 21.548414549502258 26.853877663282294 29.097908338394863 22.499799448820585 78-79 21.525417310656906 26.79193709274157 29.016979419217964 22.66566617738356 80-81 21.419169111316602 26.989134593922387 29.025850180160074 22.565846114600937 82-83 21.49303635049954 26.905199691058122 29.195854199367165 22.40590975907517 84-85 21.363306397168838 26.762762625112664 28.929032116630488 22.94489886108801 86-87 21.42656425627576 26.68617419864002 29.057520148593387 22.829741396490835 88-89 21.521497482124254 26.33249113499471 28.996900424454015 23.149110958427023 90-91 21.379028235954145 26.191810771126395 29.578072505382274 22.85108848753719 92-93 21.487858618452588 26.03279224370658 29.48491682353914 22.994432314301687 94-95 21.108660729945626 26.152000255114245 29.468236283623263 23.271102731316866 96-97 21.038353731993194 26.138749415840707 29.66193991703692 23.160956935129175 98-99 21.13358839288072 26.457155127788024 29.269004784483982 23.140251694847276 100-101 20.902205966977007 26.56970316573724 29.350515931319432 23.177574935966323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1181.0 1 677.5 2 176.5 3 188.5 4 172.0 5 157.0 6 240.5 7 536.0 8 914.5 9 1160.0 10 1201.0 11 1139.5 12 1193.0 13 1310.0 14 1387.5 15 1472.0 16 1567.0 17 1652.0 18 1806.5 19 1942.0 20 2074.5 21 2341.0 22 2750.5 23 3254.0 24 3942.0 25 4908.0 26 6203.0 27 7714.5 28 9003.0 29 10543.0 30 12578.0 31 14642.0 32 16692.0 33 18670.0 34 21208.0 35 23390.0 36 25313.0 37 27561.0 38 29284.0 39 30302.0 40 31190.5 41 33207.0 42 35214.5 43 36900.0 44 38335.5 45 39058.5 46 39182.0 47 39929.5 48 42054.5 49 41510.5 50 38677.5 51 36268.5 52 33784.0 53 32237.5 54 30670.0 55 27639.5 56 24469.5 57 21434.0 58 18641.0 59 16111.5 60 13406.0 61 10744.0 62 8344.0 63 6363.0 64 4784.0 65 3639.0 66 2666.0 67 2005.5 68 1484.5 69 976.0 70 670.5 71 489.0 72 353.5 73 246.5 74 181.5 75 159.0 76 113.0 77 62.5 78 34.0 79 23.0 80 17.5 81 13.0 82 10.0 83 6.0 84 3.0 85 3.0 86 3.0 87 1.5 88 2.5 89 2.5 90 2.0 91 1.5 92 1.5 93 1.5 94 0.5 95 0.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24234224375491198 2 0.22115221997831255 3 0.22383827933027586 4 0.2252310508461087 5 0.2172723564699211 6 0.17340005372118703 7 0.1786726887454113 8 0.17359902108059172 9 0.17240521692416358 10-11 0.184343258488445 12-13 0.18528835344561725 14-15 0.17340005372118703 16-17 0.18155771545677932 18-19 0.1684258697360698 20-21 0.17524050179568043 22-23 0.17369850476029408 24-25 0.17006735045115848 26-27 0.17558869467463864 28-29 0.17066425252937256 30-31 0.17374824660014526 32-33 0.17494205075657338 34-35 0.16832638605636746 36-37 0.170216575970712 38-39 0.1691222554939862 40-41 0.16942070653309324 42-43 0.16598851958336236 44-45 0.17499179259642456 46-47 0.16524239198559476 48-49 0.1684258697360698 50-51 0.1736487629204429 52-53 0.1734497955610382 54-55 0.17752862642883435 56-57 0.16474497358708304 58-59 0.1592236293636029 60-61 0.16479471542693422 62-63 0.1660382614232135 64-65 0.1638993623096131 66-67 0.17071399436922374 68-69 0.16459574806752952 70-71 0.16887354629473036 72-73 0.17648404779195973 74-75 0.16658542166157642 76-77 0.16966941573234912 78-79 0.16723206557964165 80-81 0.1629542673524408 82-83 0.17558869467463864 84-85 0.16360091127050608 86-87 0.1637501367900596 88-89 0.16569006854425533 90-91 0.16559058486455297 92-93 0.17121141276773547 94-95 0.1709129617286284 96-97 0.16026820800047753 98-99 0.16250659079378027 100-101 0.16081536823884043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1005190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.64716419515933 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97217020066493 54.468876807917574 2 10.269840449468452 13.483718044886889 3 2.9115332385688393 5.734017017159779 4 1.2251733920754535 3.2171663534847053 5 0.682204366323795 2.2392391025356404 6 0.41354823066527696 1.6288961160660638 7 0.2728352731462376 1.2537603382123546 8 0.20123929344245334 1.0568631151307668 9 0.14940754861517047 0.8827363688342712 >10 0.8257986044170443 9.869813478132999 >50 0.05351838908103289 2.3990317581802914 >100 0.02151869335074487 2.334936331985895 >500 6.061600902824871E-4 0.2606421258883921 >1k 6.061600902824871E-4 1.1703030415843683 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4652 0.4627980779753082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3650 0.36311543091355863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.224733632447597 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1203 0.11967886668192082 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.487091992558621E-4 0.0 24-25 0.0 0.0 0.0 5.96902078214069E-4 0.0 26-27 0.0 0.0 0.0 0.0010943204767257931 0.0 28-29 0.0 0.0 0.0 0.0023378664730051035 0.0 30-31 0.0 0.0 0.0 0.006963857579164138 0.0 32-33 0.0 0.0 0.0 0.012087267083834896 0.0 34-35 0.0 0.0 0.0 0.01726041842835683 0.0 36-37 0.0 0.0 0.0 0.02511962912484207 0.0 38-39 0.0 0.0 0.0 0.033227549020583176 0.0 40-41 0.0 0.0 0.0 0.04034063211930083 0.0 42-43 0.0 0.0 0.0 0.060933753817686204 0.0 44-45 0.0 0.0 0.0 0.0845611277469931 0.0 46-47 0.0 0.0 0.0 0.10490554024612263 0.0 48-49 0.0 0.0 0.0 0.13325838896129089 0.0 50-51 0.0 0.0 0.0 0.17101244540833077 0.0 52-53 0.0 0.0 0.0 0.24249146927446552 0.0 54-55 0.0 0.0 0.0 0.29760542782956456 0.0 56-57 0.0 0.0 0.0 0.3998249087237239 0.0 58-59 0.0 0.0 0.0 0.5314915588097773 0.0 60-61 0.0 0.0 0.0 0.6622131139386584 0.0 62-63 0.0 0.0 0.0 0.8616778917418597 0.0 64-65 0.0 0.0 0.0 0.9885195833623495 0.0 66-67 0.0 0.0 0.0 1.2304141505586008 0.0 68-69 0.0 0.0 0.0 1.4993682786338902 0.0 70-71 0.0 0.0 0.0 1.7061451068952138 0.0 72-73 0.0 0.0 0.0 2.084929217361892 0.0 74-75 0.0 0.0 0.0 2.46033088271869 0.0 76-77 0.0 0.0 0.0 2.7141634914792228 0.0 78-79 0.0 0.0 0.0 2.9979904296700126 0.0 80-81 0.0 0.0 0.0 3.470338940896746 0.0 82-83 0.0 0.0 0.0 3.8908564549985574 0.0 84-85 0.0 0.0 0.0 4.444433390702255 0.0 86-87 0.0 0.0 0.0 5.186780608641152 0.0 88-89 0.0 0.0 0.0 6.2130542484505416 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 39.52868 1 GTATCAA 5030 0.0 35.063725 1 TATCAAC 6160 0.0 28.385899 2 ATCAACG 6165 0.0 28.285805 3 TCAACGC 6145 0.0 28.224623 4 CAACGCA 6200 0.0 27.977036 5 AACGCAG 6355 0.0 27.357174 6 ACGCAGA 7115 0.0 24.368212 7 CGCAGAG 7140 0.0 24.282888 8 GTACATG 4025 0.0 23.976303 1 GAGTACT 4050 0.0 22.3421 12-13 GCAGAGT 7660 0.0 22.138344 9 GGGCGCG 215 4.418871E-8 22.093637 7 TTAGGGC 310 1.0913936E-11 21.458628 3 TACATGG 4270 0.0 21.365295 2 AGACGGC 135 3.7947687E-4 21.111698 6 GTGTAGC 500 0.0 20.91726 1 GTATAGC 455 0.0 20.896362 1 ACATGGG 4190 0.0 20.866009 3 GGACCGT 115 0.0037223543 20.652748 6 >>END_MODULE