FastQCFastQC Report
Thu 26 May 2016
SRR937401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.4237009918522866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.3523711935057054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.2318964573861658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10840.1078403087973418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.032.8460961
GTATCAA55250.029.5056381
GTACATG39800.024.7189731
ATCAACG66850.024.2338963
TCAACGC67650.023.9473154
TATCAAC67800.023.8943352
CCTAGAC2202.1627784E-923.754193
CAACGCA68250.023.7367925
AACGCAG70300.023.106446
TACATGG42200.022.4032422
ACATGGG40750.022.1511483
GAGTACT41450.021.60406512-13
ACGCAGA77550.020.8279137
ATATTCG2309.0056346E-820.654799
CGCAGAG77650.020.5563728
TAAGGTG4100.019.6985995
GTACTTT45350.019.68986714-15
AGTACTT44050.019.62791312-13
AGAGTAC66450.019.37416310-11
GCAGAGT81650.019.2002289
Produced by FastQC (version 0.11.5)