##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937401_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1005190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15565912911987 33.0 31.0 34.0 31.0 34.0 2 32.38513415374208 34.0 31.0 34.0 31.0 34.0 3 32.46554681204548 34.0 31.0 34.0 31.0 34.0 4 36.07848665426437 37.0 35.0 37.0 35.0 37.0 5 35.959506163013955 37.0 35.0 37.0 35.0 37.0 6 36.039325898586334 37.0 35.0 37.0 35.0 37.0 7 35.98935922561904 37.0 35.0 37.0 35.0 37.0 8 35.97029914742487 37.0 35.0 37.0 35.0 37.0 9 37.67380395746078 39.0 37.0 39.0 35.0 39.0 10-11 37.596237029815256 39.0 37.0 39.0 35.0 39.0 12-13 37.52293049075299 39.0 37.0 39.0 35.0 39.0 14-15 38.74627931037913 40.0 38.0 41.0 35.0 41.0 16-17 38.72743759886191 40.0 38.0 41.0 35.0 41.0 18-19 38.71793839970553 40.0 38.0 41.0 34.5 41.0 20-21 38.6107298122743 40.0 38.0 41.0 34.0 41.0 22-23 38.482561008366574 40.0 38.0 41.0 34.0 41.0 24-25 38.3551811100389 40.0 38.0 41.0 34.0 41.0 26-27 38.30814622111242 40.0 38.0 41.0 34.0 41.0 28-29 38.207402083188256 40.0 38.0 41.0 34.0 41.0 30-31 38.29989355246272 40.0 38.0 41.0 34.0 41.0 32-33 38.23988449944787 40.0 38.0 41.0 34.0 41.0 34-35 38.13518091107154 40.0 38.0 41.0 34.0 41.0 36-37 37.99295605805868 40.0 37.0 41.0 33.0 41.0 38-39 37.87306578855738 40.0 37.0 41.0 33.0 41.0 40-41 37.71018961589351 40.0 37.0 41.0 33.0 41.0 42-43 37.51468428854247 40.0 37.0 41.0 33.0 41.0 44-45 37.22414518648216 40.0 37.0 41.0 32.0 41.0 46-47 36.880920025069884 40.0 36.0 41.0 31.0 41.0 48-49 36.59717764800685 40.0 36.0 41.0 30.5 41.0 50-51 36.373287139744725 39.0 36.0 41.0 30.0 41.0 52-53 36.14001731016027 39.0 35.5 41.0 30.0 41.0 54-55 35.90753489390066 39.0 35.0 40.0 29.0 41.0 56-57 35.66653319272973 39.0 35.0 40.0 28.5 41.0 58-59 35.42742516340194 38.0 35.0 40.0 28.0 41.0 60-61 35.10728916921179 38.0 34.5 40.0 27.5 41.0 62-63 34.75339637282504 38.0 34.0 40.0 26.5 41.0 64-65 34.40055362667755 37.0 34.0 40.0 26.0 41.0 66-67 33.994250340731604 36.5 33.5 39.0 26.0 41.0 68-69 33.588018683035045 36.0 33.0 39.0 25.5 40.0 70-71 33.18300271590445 35.5 33.0 39.0 25.0 40.0 72-73 32.73740337647609 35.0 32.0 38.0 24.0 40.0 74-75 32.28247793949403 35.0 32.0 37.0 22.5 39.0 76-77 30.93293158507347 34.0 30.5 36.0 21.0 38.5 78-79 31.592502412479234 34.5 31.5 36.0 22.0 39.0 80-81 31.45037654572767 35.0 31.5 36.0 21.5 37.5 82-83 31.11903470985585 34.5 31.0 35.5 20.0 37.0 84-85 30.770453347128402 34.0 31.0 35.0 19.5 37.0 86-87 30.40483639908873 34.0 31.0 35.0 18.0 36.0 88-89 30.0330872770322 34.0 30.5 35.0 15.0 36.0 90-91 29.617369353057633 34.0 30.0 35.0 7.5 35.5 92-93 29.264572866821197 34.0 29.5 35.0 4.0 35.0 94-95 28.871352182174512 34.0 29.0 35.0 2.0 35.0 96-97 28.402962623981537 34.0 29.0 35.0 2.0 35.0 98-99 27.941727932032748 34.0 29.0 35.0 2.0 35.0 100-101 26.097862095723197 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08180834087664124 1101 2 0.1016438755366309 1101 3 0.04096008659941219 1101 4 -0.006049266804225795 1101 5 -0.07565523955877751 1101 6 0.004629007566471444 1101 7 -0.019341546252675812 1101 8 -0.03171671364120954 1101 9 0.04579418041328864 1101 10-11 -0.05920600867172965 1101 12-13 -0.03344884068149412 1101 14-15 -0.1724487026776984 1101 16-17 -0.03190107996088898 1101 18-19 -0.1555538434888959 1101 20-21 -0.12606750707882952 1101 22-23 -0.03674981451949577 1101 24-25 0.14283246580692577 1101 26-27 -0.015884109417562797 1101 28-29 -0.036702875411208424 1101 30-31 -0.03598242491555226 1101 32-33 -0.056601653851089395 1101 34-35 0.09220687105123204 1101 36-37 0.06926479454670442 1101 38-39 -0.10347821294315196 1101 40-41 -0.10893076978546645 1101 42-43 -0.11871968680981837 1101 44-45 -0.026787498890698203 1101 46-47 -0.011959357883739585 1101 48-49 -0.044849526216417246 1101 50-51 -0.07895262051423657 1101 52-53 -0.2931191601016465 1101 54-55 -0.42766180990714986 1101 56-57 -0.3095598673298312 1101 58-59 -0.37450332347685844 1101 60-61 -0.3828990772275773 1101 62-63 -0.42684407764566146 1101 64-65 -0.2957627890518566 1101 66-67 -0.21175300462903834 1101 68-69 -0.31726275028584894 1101 70-71 -0.5172904673495893 1101 72-73 -0.28197245093544865 1101 74-75 -0.4078095664642234 1101 76-77 -0.31821090362934257 1101 78-79 -0.3128299657283762 1101 80-81 -0.344536033350888 1101 82-83 -0.16972681887545704 1101 84-85 -0.39808580051899156 1101 86-87 -0.4702779207066605 1101 88-89 -0.47437017564038797 1101 90-91 -0.3363374089536286 1101 92-93 -0.48436696374028543 1101 94-95 -0.42396987608597314 1101 96-97 -0.3848748449552879 1101 98-99 -0.22282515299726668 1101 100-101 0.22463239031963766 1102 1 0.028568468798859215 1102 2 0.07168179881147552 1102 3 0.0767380900753949 1102 4 0.07843899098353546 1102 5 -0.12957092548422366 1102 6 0.0117363663382406 1102 7 0.04202986490042804 1102 8 0.07248584096074495 1102 9 0.17416721941111746 1102 10-11 -0.013790080042454633 1102 12-13 0.0309468694588233 1102 14-15 -0.0016897974534373361 1102 16-17 -0.10454893398509313 1102 18-19 -0.01023764329601562 1102 20-21 -0.045957097112129475 1102 22-23 -0.024254942892099507 1102 24-25 -0.03869804977116331 1102 26-27 0.031063393550596174 1102 28-29 0.03060634806032425 1102 30-31 -0.02783551673188356 1102 32-33 -0.022156280144812968 1102 34-35 -0.06744144959618126 1102 36-37 -0.10909562897469272 1102 38-39 -0.20149299375619023 1102 40-41 -0.29451830304784465 1102 42-43 -0.16326277816080648 1102 44-45 -0.20872529584001853 1102 46-47 -0.18739730276072208 1102 48-49 -0.13857397774203406 1102 50-51 -0.2143926743744231 1102 52-53 -0.36571002034906996 1102 54-55 -0.17509378132402276 1102 56-57 -0.0850626613056491 1102 58-59 -0.1716458220071857 1102 60-61 -0.3221524630207995 1102 62-63 -0.2909213910913664 1102 64-65 -0.18547470792345422 1102 66-67 -0.030774282732629388 1102 68-69 -0.11556412227761115 1102 70-71 -0.16337725755827393 1102 72-73 -0.20634555140971145 1102 74-75 -0.12248292381605808 1102 76-77 -0.19965573642838663 1102 78-79 -0.08383323977537316 1102 80-81 0.09284893847882003 1102 82-83 0.06957041156852384 1102 84-85 -0.06048241658287168 1102 86-87 0.17620782593956363 1102 88-89 0.15354541892424223 1102 90-91 0.2760088519344208 1102 92-93 0.16514052037510396 1102 94-95 0.16442491020017158 1102 96-97 0.13055573982647672 1102 98-99 0.14352358658642572 1102 100-101 0.5849850430490839 1103 1 0.11679658224943523 1103 2 0.08555022555043479 1103 3 0.057463446397420626 1103 4 0.12840124708640133 1103 5 0.00817325227409782 1103 6 -0.034110542169990765 1103 7 0.007664677962900157 1103 8 0.06386507873046554 1103 9 0.05128908589594516 1103 10-11 -0.05098184990851706 1103 12-13 -0.011064613665858758 1103 14-15 0.040656329517588574 1103 16-17 0.043264024659379174 1103 18-19 0.020024122557124713 1103 20-21 0.051943416148745314 1103 22-23 -0.041720264809661955 1103 24-25 -0.04101249431850107 1103 26-27 -0.017855643685372513 1103 28-29 -0.1531467984336956 1103 30-31 -0.144915963148172 1103 32-33 -0.06251559082058122 1103 34-35 0.016780759530270473 1103 36-37 -0.20890508543451602 1103 38-39 -0.17808756817620974 1103 40-41 -0.07805915765947447 1103 42-43 -0.1259257854935072 1103 44-45 -0.1489622545518472 1103 46-47 -0.08346548311200763 1103 48-49 0.003622296135539216 1103 50-51 -0.18973917457507383 1103 52-53 -0.05872164659188428 1103 54-55 -0.1516609949264165 1103 56-57 -0.27370848323864294 1103 58-59 -0.12530010474685582 1103 60-61 -0.16336430871140806 1103 62-63 -0.3227251845634953 1103 64-65 -0.3501847253501893 1103 66-67 -0.22905181402919794 1103 68-69 -0.12668243606549368 1103 70-71 -0.2464110250165774 1103 72-73 -0.1503512873595838 1103 74-75 -0.016623920423320726 1103 76-77 -0.07846274865516634 1103 78-79 -0.07219647357145575 1103 80-81 -0.13935109575728077 1103 82-83 -0.20871716605902435 1103 84-85 -0.07923790628538185 1103 86-87 -0.18336453149654375 1103 88-89 -0.17479351285331646 1103 90-91 -0.15110263147088787 1103 92-93 0.0015635214218292504 1103 94-95 -0.014114246637646488 1103 96-97 0.14810945823174748 1103 98-99 0.11334951566157514 1103 100-101 0.2856170077802851 1104 1 0.04737987230082297 1104 2 0.1593488962302203 1104 3 0.0766822422668767 1104 4 0.09464778318677247 1104 5 0.06730552726982353 1104 6 -0.02629237149611896 1104 7 0.014932818473837983 1104 8 -0.003861215937313034 1104 9 0.1134575488976779 1104 10-11 -0.009794376186839315 1104 12-13 0.014506412272119462 1104 14-15 0.07634484576955458 1104 16-17 0.020584286308135802 1104 18-19 0.057674843188046054 1104 20-21 0.01351254520850631 1104 22-23 0.008928480444112097 1104 24-25 -0.007720614871345788 1104 26-27 0.01830682328862565 1104 28-29 0.11560154152903124 1104 30-31 0.05814140881523855 1104 32-33 0.1569980599280072 1104 34-35 0.18255221581443237 1104 36-37 0.08831551483496725 1104 38-39 0.03454828604806437 1104 40-41 0.172133057182414 1104 42-43 0.2096017541887747 1104 44-45 0.15409361195266058 1104 46-47 0.27942108378574204 1104 48-49 0.2913240948363338 1104 50-51 0.34839060458485704 1104 52-53 0.43606516429775155 1104 54-55 0.44594677711269526 1104 56-57 0.26239215238761204 1104 58-59 0.2991395688266323 1104 60-61 0.57903285500403 1104 62-63 0.6472599974196029 1104 64-65 0.43123635257988724 1104 66-67 0.5884682865354591 1104 68-69 0.5266727479284654 1104 70-71 0.5146156549411387 1104 72-73 0.49982361470493686 1104 74-75 0.4895847533856781 1104 76-77 0.5303609610321409 1104 78-79 0.5199663762192337 1104 80-81 0.5810386783055961 1104 82-83 0.8003392445613429 1104 84-85 0.8227744621348236 1104 86-87 0.775186690129587 1104 88-89 0.8276235551794464 1104 90-91 0.7374893773381963 1104 92-93 0.6491323665950617 1104 94-95 0.8057648542592837 1104 96-97 0.8696347784374794 1104 98-99 0.9638741143223619 1104 100-101 1.1794541967014034 1105 1 -0.060244242862054875 1105 2 -0.03366219933376868 1105 3 0.04518664146603868 1105 4 0.06672881221545879 1105 5 -0.02913497645672436 1105 6 -0.05766978248887966 1105 7 -0.05193722984913762 1105 8 -0.06741322421218143 1105 9 0.07134866197597489 1105 10-11 -0.0344262426973998 1105 12-13 -0.05800660447116712 1105 14-15 -0.0860891979717735 1105 16-17 -0.10886805860938154 1105 18-19 -0.13091127355493626 1105 20-21 -0.09559132221583866 1105 22-23 -0.13600672933090152 1105 24-25 -0.22029172706123745 1105 26-27 -0.15146490380555377 1105 28-29 -0.2180046595491163 1105 30-31 -0.057727833596025846 1105 32-33 -0.18183347914126102 1105 34-35 -0.37689487798544263 1105 36-37 -0.09566318244365135 1105 38-39 -0.37178946393528634 1105 40-41 -0.13095875646795463 1105 42-43 -0.19328033204377704 1105 44-45 0.07951029051763214 1105 46-47 0.0876521710367939 1105 48-49 0.0026343835367512725 1105 50-51 -0.1016793574357564 1105 52-53 -0.04627696108815371 1105 54-55 0.16867666025549966 1105 56-57 -0.02523662690997952 1105 58-59 0.020743079301901446 1105 60-61 0.04699865769526923 1105 62-63 -0.19672866270458655 1105 64-65 -0.2793176006708862 1105 66-67 -0.21347335784171406 1105 68-69 -0.10095796682638536 1105 70-71 -0.13019462265419435 1105 72-73 -0.09519821471464951 1105 74-75 -0.22537910750254042 1105 76-77 -0.15087954015610094 1105 78-79 -0.03914121934000647 1105 80-81 0.05233838544870295 1105 82-83 0.06103462897171141 1105 84-85 0.08385376511638043 1105 86-87 0.1186526181496177 1105 88-89 0.35294552325392203 1105 90-91 0.40270524428026633 1105 92-93 0.3149126097011816 1105 94-95 -0.020887582037200758 1105 96-97 0.2515088245902035 1105 98-99 0.5348954805013548 1105 100-101 1.2327367798070803 1106 1 -0.3563662954529043 1106 2 -0.1569678074186811 1106 3 -0.0871693896723329 1106 4 -9.04650775694904E-4 1106 5 -0.219774245863114 1106 6 -0.2445218137092553 1106 7 -0.14755346321270935 1106 8 -0.09085284620584133 1106 9 -0.25074338994865286 1106 10-11 -0.3695852913267643 1106 12-13 -0.3529576368651206 1106 14-15 -0.37198212008996023 1106 16-17 -0.19796873047260988 1106 18-19 -0.254933715119229 1106 20-21 -0.2914867298692414 1106 22-23 -0.04067684499797508 1106 24-25 0.020970463592128397 1106 26-27 0.023577632157831374 1106 28-29 0.1450372255627883 1106 30-31 0.004256161607145259 1106 32-33 -0.04547176458319768 1106 34-35 -0.008966481898760037 1106 36-37 0.16293881930902643 1106 38-39 0.06794847865653963 1106 40-41 0.19041454017170878 1106 42-43 -0.23138717418409982 1106 44-45 -0.21207373850931788 1106 46-47 -0.08013939249297408 1106 48-49 -0.08424468108203342 1106 50-51 0.0855121927694924 1106 52-53 -0.03052456139359805 1106 54-55 0.10204894218180982 1106 56-57 -0.0010129082418117719 1106 58-59 -0.04160029354375183 1106 60-61 -0.08003140959355193 1106 62-63 -0.13040202750428165 1106 64-65 -0.2030859795382014 1106 66-67 -0.48017512949021324 1106 68-69 -0.26441906179432806 1106 70-71 -0.2672629210619206 1106 72-73 -0.340262377396769 1106 74-75 -0.43053505561891114 1106 76-77 -0.4657186978415311 1106 78-79 -0.07625769100746638 1106 80-81 0.04021307347559855 1106 82-83 -0.12065479187296191 1106 84-85 -0.5351719819535106 1106 86-87 -0.2775775680349071 1106 88-89 -0.09238679536606753 1106 90-91 -0.28057222857102815 1106 92-93 -0.3010664349308314 1106 94-95 -0.8981322509485388 1106 96-97 -0.5119393302202049 1106 98-99 -0.3471472963283304 1106 100-101 -0.07008276521185763 1107 1 -0.15064728373159397 1107 2 -0.04890738514723836 1107 3 -0.013924972268135605 1107 4 -0.04043199575112055 1107 5 -0.09541996373407358 1107 6 -0.055315307572911365 1107 7 0.007268625709556886 1107 8 -0.1081429700733949 1107 9 -0.06355222703857066 1107 10-11 -0.07239156170597738 1107 12-13 -0.11320404109354598 1107 14-15 -0.19284947818412945 1107 16-17 -0.1198637234854516 1107 18-19 -0.018941776492134466 1107 20-21 -0.19474550184075667 1107 22-23 -0.19600657226119722 1107 24-25 -0.13667880964849388 1107 26-27 -0.10995216545227748 1107 28-29 -0.23025295534279167 1107 30-31 -0.30585477733365707 1107 32-33 -0.1226708177521374 1107 34-35 0.10028603490369647 1107 36-37 -0.13865159069923294 1107 38-39 -0.22653689584556957 1107 40-41 0.07047214856770267 1107 42-43 -7.615622137251421E-4 1107 44-45 0.027818649767468173 1107 46-47 0.18266991991374226 1107 48-49 0.2148693284052854 1107 50-51 0.3528018413202503 1107 52-53 0.1237242954695077 1107 54-55 0.2839469772221648 1107 56-57 0.12903566913500697 1107 58-59 0.2612953363936015 1107 60-61 0.21647216003560033 1107 62-63 0.15020219521015576 1107 64-65 0.26539386227636896 1107 66-67 0.2944068293853732 1107 68-69 0.18035387937095493 1107 70-71 0.07601277218763158 1107 72-73 0.14378884274145776 1107 74-75 0.050178489959215256 1107 76-77 0.12593854170862784 1107 78-79 0.06972065355918744 1107 80-81 0.3901042259287095 1107 82-83 0.4383576899461694 1107 84-85 0.5049647191577833 1107 86-87 0.4143363425865232 1107 88-89 0.2256201062338512 1107 90-91 0.3518225125239134 1107 92-93 0.320838064417984 1107 94-95 0.17403985175791803 1107 96-97 0.0458582418615876 1107 98-99 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94-95 -0.10267770549399202 2316 96-97 -0.18022752034058342 2316 98-99 -0.18493009001866056 2316 100-101 -0.3776144173496725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 4.0 5 18.0 6 46.0 7 113.0 8 245.0 9 493.0 10 809.0 11 1266.0 12 1972.0 13 2610.0 14 3491.0 15 4841.0 16 6749.0 17 9527.0 18 8683.0 19 5335.0 20 4448.0 21 4497.0 22 4758.0 23 5162.0 24 6020.0 25 6938.0 26 8352.0 27 10525.0 28 13505.0 29 16678.0 30 21089.0 31 26616.0 32 33823.0 33 44805.0 34 61917.0 35 90645.0 36 142568.0 37 228955.0 38 206013.0 39 21667.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61793300332366 20.05201982447042 15.066139633312995 22.263907538892934 2 15.555865060336851 21.167341497627316 39.49900018901899 23.777793253016842 3 19.239447268675576 25.12888110705439 30.13897870054418 25.492692923725862 4 13.199693590266518 17.611894268745214 37.13278086729872 32.05563127368955 5 13.662961057775064 37.800693097776936 34.11830022894315 14.418045615504854 6 31.64993682786339 36.92645171559606 17.245893811120286 14.177717645420268 7 28.167411136203107 33.381251305723296 20.72374376983456 17.72759378823904 8 26.481461216287467 35.43399755270148 19.508351654911014 18.57618957610004 9 26.438782717695165 17.051104766263094 20.244530884708364 36.26558163133338 10-11 24.67205204986122 27.339905888439002 27.27951929485968 20.7085227668401 12-13 25.76955180403576 24.489908601888448 28.101860096322685 21.6386794977531 14-15 22.511366010405993 25.95877396313135 26.390732100398928 25.139127926063733 16-17 21.764392801360938 29.512778678657764 26.861488872750428 21.86133964723087 18-19 21.66694853709249 27.744356788268888 29.623404530486773 20.96529014415185 20-21 22.393925526517375 26.77976302987495 29.692744655239306 21.13356678836837 22-23 22.11650333795439 26.90424565115852 29.633951679373325 21.34529933151376 24-25 22.159746315985824 27.055897531555058 29.49991916930921 21.28443698314991 26-27 21.99849778149187 27.205077697526814 29.503322787958375 21.29310173302294 28-29 21.618665259264308 27.38042593155428 29.69448193151051 21.3064268776709 30-31 21.885364955878988 27.079606840497817 29.650165640326705 21.38486256329649 32-33 21.64327142132333 27.26773047881495 29.551030153503316 21.5379679463584 34-35 22.07726897402481 27.15218018484068 29.55267163421841 21.217879206916106 36-37 22.08985365950716 26.98798237149196 29.417572797182622 21.504591171818262 38-39 21.990220754285257 27.21928192679991 29.545011390881328 21.245485928033506 40-41 22.02160785523135 27.195107392632238 29.451248022761867 21.332036729374547 42-43 21.87997492637109 27.337369656929077 29.126800923346334 21.655854493353498 44-45 21.98091903023309 27.530914553467507 28.84036848754962 21.64779792874979 46-47 22.141435947432825 27.758831663665575 28.409803121797868 21.68992926710373 48-49 22.278922392781464 27.890896248470437 28.154229548642544 21.675951810105552 50-51 22.263402938747898 27.942130343517146 28.069270486176745 21.725196231558215 52-53 22.397357713467105 28.30375351923517 27.662133526994896 21.63675524030283 54-55 22.100398929555606 28.05146290751002 27.87990330186333 21.968234861071043 56-57 22.15790049642356 28.303057133477257 27.864980749907975 21.674061620191207 58-59 22.275539947671586 27.945662014146578 27.9168117470329 21.861986291148938 60-61 22.15984042817776 27.80439518896925 28.41542394970105 21.62034043315194 62-63 22.03817188790179 28.035993195316312 28.317183815994984 21.608651100786915 64-65 21.947591997532808 28.037734159711096 28.18546742406908 21.829206418687015 66-67 21.760861130731506 28.26903371501905 27.994607984560133 21.975497169689312 68-69 21.831345317800615 27.997841204150458 28.186611486385658 21.98420199166327 70-71 21.84263671544683 28.141097702921837 28.043653438653386 21.972612142977948 72-73 21.88417115172256 27.992518827286382 27.96819506759916 22.155114953391898 74-75 21.880490255573573 28.191585670370777 27.745003432186948 22.1829206418687 76-77 21.828559774768948 28.031964106288363 27.910991951770313 22.228484167172375 78-79 21.786030501696196 27.999333459345994 27.777236144410512 22.4373998945473 80-81 21.87581452262756 28.235209263920254 27.654771734696926 22.23420447875526 82-83 21.68391228263908 28.308676131609868 27.868308556750364 22.13910302900069 84-85 21.509857994735242 28.28639618613295 27.626211979219516 22.577533839912295 86-87 21.593877774351117 27.911986788567333 27.571304927426652 22.92283050965489 88-89 21.57238929953541 27.927257533401644 27.617415612968692 22.88293755409425 90-91 21.54184283484889 27.68500706165153 27.875639317007213 22.897510786492372 92-93 21.461831368091705 27.632582305115267 27.91556715107531 22.99001917571772 94-95 21.183557337418797 27.691879147225894 27.97287080054517 23.151692714810135 96-97 21.13361653020822 27.806285378883594 27.96018663138312 23.099911459525067 98-99 20.98033548998121 28.08259959718918 27.54225210943442 23.39481280339519 100-101 20.559783373853726 28.644260805064825 27.487949598502365 23.308006222579085 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 128.5 2 87.0 3 117.5 4 128.5 5 145.0 6 220.0 7 433.5 8 694.5 9 940.0 10 1047.0 11 1000.5 12 1109.5 13 1255.0 14 1387.5 15 1524.0 16 1566.0 17 1636.0 18 1739.0 19 1866.5 20 2060.5 21 2281.0 22 2673.0 23 3270.0 24 3888.5 25 4732.0 26 6079.0 27 7653.0 28 8920.0 29 10470.5 30 12624.0 31 14513.5 32 16581.5 33 18667.5 34 21283.0 35 23282.5 36 25175.5 37 27675.5 38 29111.5 39 29948.0 40 30879.5 41 33006.0 42 35118.5 43 36884.5 44 38788.5 45 39586.0 46 38880.5 47 39596.5 48 42561.0 49 42025.5 50 39043.5 51 36758.5 52 34279.5 53 32264.0 54 30352.0 55 27770.5 56 24448.0 57 21261.5 58 18797.5 59 16460.0 60 13931.5 61 11240.5 62 8745.5 63 6604.0 64 4723.0 65 3537.0 66 2606.5 67 2150.0 68 1599.5 69 922.0 70 655.0 71 482.0 72 372.5 73 268.5 74 192.5 75 156.0 76 106.5 77 54.5 78 36.0 79 19.5 80 8.0 81 4.0 82 2.5 83 2.0 84 1.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05700414846944359 2 0.0 3 0.0 4 0.0 5 0.01362926411922124 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0026860593519633102 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.45611277469931E-4 24-25 2.487091992558621E-4 26-27 9.948367970234484E-4 28-29 0.0028352848715168274 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014922551955351724 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.963857579164138E-4 84-85 0.0015917388752375173 86-87 0.0 88-89 0.0 90-91 0.011291397646216138 92-93 0.0012435459962793103 94-95 0.0 96-97 0.0 98-99 3.481928789582069E-4 100-101 0.01397745699817945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1005190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.85195240214758 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3127924936058 51.106677634938144 2 11.02755352288752 13.862065382653224 3 3.2860536060062824 6.196046545068369 4 1.3982786826285782 3.5153818082203614 5 0.7593845373350543 2.3864400397754846 6 0.48788943970871446 1.8398882305249542 7 0.31146535481172716 1.370334395888128 8 0.22467007903218555 1.129676249081413 9 0.17649467482587688 0.9983731411249676 >10 0.9230883407926476 10.452798543993143 >50 0.06309631249704549 2.752753592896591 >100 0.028279707533312125 3.1199969683683744 >500 3.177494450763647E-4 0.14958408575313611 >1k 6.354988901527294E-4 1.1199833817137346 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.4237009918522866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3542 0.3523711935057054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2331 0.2318964573861658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1084 0.1078403087973418 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.948367970234483E-5 0.0 20-21 0.0 0.0 0.0 9.948367970234483E-5 0.0 22-23 0.0 0.0 0.0 3.481928789582069E-4 0.0 24-25 0.0 0.0 0.0 6.963857579164138E-4 0.0 26-27 0.0 0.0 0.0 0.0012932878361304827 0.0 28-29 0.0 0.0 0.0 0.0025865756722609655 0.0 30-31 0.0 0.0 0.0 0.00741153413782469 0.0 32-33 0.0 0.0 0.0 0.012634427322197793 0.0 34-35 0.0 0.0 0.0 0.017757836826868552 0.0 36-37 0.0 0.0 0.0 0.02571653120305614 0.0 38-39 0.0 0.0 0.0 0.03372496741909489 0.0 40-41 0.0 0.0 0.0 0.04063908315840786 0.0 42-43 0.0 0.0 0.0 0.06148091405604911 0.0 44-45 0.0 0.0 0.0 0.08530725534476069 0.0 46-47 0.0 0.0 0.0 0.10565166784389021 0.0 48-49 0.0 0.0 0.0 0.13415374207861203 0.0 50-51 0.0 0.0 0.0 0.17215650772490773 0.0 52-53 0.0 0.0 0.0 0.24338682239178666 0.0 54-55 0.0 0.0 0.0 0.298301813587481 0.0 56-57 0.0 0.0 0.0 0.400720261841045 0.0 58-59 0.0 0.0 0.0 0.5324366537669496 0.0 60-61 0.0 0.0 0.0 0.6634566599349376 0.0 62-63 0.0 0.0 0.0 0.8637173071757578 0.0 64-65 0.0 0.0 0.0 0.991603577433122 0.0 66-67 0.0 0.0 0.0 1.234791432465504 0.0 68-69 0.0 0.0 0.0 1.5072274893303752 0.0 70-71 0.0 0.0 0.0 1.7155960564669366 0.0 72-73 0.0 0.0 0.0 2.0968672589261734 0.0 74-75 0.0 0.0 0.0 2.475352918353744 0.0 76-77 0.0 0.0 0.0 2.731523393587282 0.0 78-79 0.0 0.0 0.0 3.020672708642147 0.0 80-81 0.0 0.0 0.0 3.4987912732916167 0.0 82-83 0.0 0.0 0.0 3.923437360101075 0.0 84-85 0.0 0.0 0.0 4.483629960504979 0.0 86-87 0.0 0.0 0.0 5.234831225937385 0.0 88-89 0.0 0.0 0.0 6.2766740616201915 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 32.846096 1 GTATCAA 5525 0.0 29.505638 1 GTACATG 3980 0.0 24.718973 1 ATCAACG 6685 0.0 24.233896 3 TCAACGC 6765 0.0 23.947315 4 TATCAAC 6780 0.0 23.894335 2 CCTAGAC 220 2.1627784E-9 23.75419 3 CAACGCA 6825 0.0 23.736792 5 AACGCAG 7030 0.0 23.10644 6 TACATGG 4220 0.0 22.403242 2 ACATGGG 4075 0.0 22.151148 3 GAGTACT 4145 0.0 21.604065 12-13 ACGCAGA 7755 0.0 20.827913 7 ATATTCG 230 9.0056346E-8 20.65479 9 CGCAGAG 7765 0.0 20.556372 8 TAAGGTG 410 0.0 19.698599 5 GTACTTT 4535 0.0 19.689867 14-15 AGTACTT 4405 0.0 19.627913 12-13 AGAGTAC 6645 0.0 19.374163 10-11 GCAGAGT 8165 0.0 19.200228 9 >>END_MODULE