FastQCFastQC Report
Thu 26 May 2016
SRR937400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241541
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.43059391514255263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38850.31291757581908286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25940.2089338974709655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15100.12162304748695371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.044.462611
GTATCAA55400.038.931751
TATCAAC67900.031.7582282
ATCAACG67550.031.7118323
TCAACGC68100.031.4595284
CAACGCA69300.030.9858235
AACGCAG71100.030.2681876
ACGCAGA79150.026.8896357
CGCAGAG79050.026.8023768
GTACATG48700.025.7532941
GTGTAGC5150.024.9065951
GAGTACT45400.024.58815812-13
GCAGAGT84600.024.53779
TACATGG51450.024.0025942
ACATGGG51150.023.029063
GTACTTT50200.021.9514514-15
CAGAGTA82050.021.13182610-11
AGAGTAC74000.020.22095110-11
AGTACTT49300.020.08968512-13
TAGCCCT6250.019.7613284
Produced by FastQC (version 0.11.5)