##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937400_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241541 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.768055183034633 33.0 31.0 34.0 30.0 34.0 2 31.863426983079897 33.0 31.0 34.0 30.0 34.0 3 31.903113952740988 33.0 31.0 34.0 30.0 34.0 4 35.50942014802572 37.0 35.0 37.0 33.0 37.0 5 35.521846640586176 37.0 35.0 37.0 33.0 37.0 6 35.54952836837446 37.0 35.0 37.0 33.0 37.0 7 35.42234529508087 37.0 35.0 37.0 33.0 37.0 8 35.43579712631319 37.0 35.0 37.0 33.0 37.0 9 37.07171893638631 39.0 37.0 39.0 33.0 39.0 10-11 36.96118009795891 39.0 37.0 39.0 33.0 39.0 12-13 36.8188340135364 39.0 37.0 39.0 33.0 39.0 14-15 38.008931642209156 40.0 38.0 41.0 33.0 41.0 16-17 37.94255445450452 40.0 38.0 41.0 33.0 41.0 18-19 37.91576113877834 40.0 38.0 41.0 33.0 41.0 20-21 37.80268593626791 40.0 38.0 41.0 32.5 41.0 22-23 37.6485146281919 40.0 37.5 41.0 32.0 41.0 24-25 37.581186606000124 40.0 37.0 41.0 32.0 41.0 26-27 37.600622532803996 40.0 37.0 41.0 32.0 41.0 28-29 37.52579495965095 40.0 37.0 41.0 32.0 41.0 30-31 37.31695368900423 40.0 37.0 41.0 32.0 41.0 32-33 37.09014361990462 39.0 36.0 41.0 31.0 41.0 34-35 37.14377213479055 40.0 36.5 41.0 31.0 41.0 36-37 37.033278401599304 40.0 36.0 41.0 31.0 41.0 38-39 36.922671905317664 40.0 36.0 41.0 31.0 41.0 40-41 36.716583664977634 39.5 36.0 41.0 30.5 41.0 42-43 36.46582432638149 39.0 36.0 41.0 30.0 41.0 44-45 36.23467287830205 39.0 35.0 41.0 30.0 41.0 46-47 35.89816566669969 39.0 35.0 41.0 28.5 41.0 48-49 35.58135937516361 39.0 35.0 41.0 27.0 41.0 50-51 34.59570847841513 38.0 34.0 39.5 25.0 40.5 52-53 34.715310650232254 38.0 34.5 39.5 26.0 40.5 54-55 35.09263044877294 39.0 35.0 40.0 26.0 41.0 56-57 34.88458375518811 38.5 34.5 40.0 25.5 41.0 58-59 34.622281100664416 38.0 34.0 40.0 24.5 41.0 60-61 34.31969504027656 38.0 34.0 40.0 23.5 41.0 62-63 34.01202578086426 38.0 34.0 40.0 23.0 41.0 64-65 33.590548761579356 37.0 33.0 40.0 22.0 41.0 66-67 33.24728381906034 36.5 33.0 39.5 21.5 41.0 68-69 32.78140472203496 36.0 32.0 39.0 19.5 40.5 70-71 32.31234973311393 35.5 32.0 39.0 18.0 40.0 72-73 31.864354056772996 35.0 31.0 38.0 15.0 40.0 74-75 31.377319798540682 35.0 31.0 37.0 10.0 39.5 76-77 30.893466667633206 35.0 30.5 37.0 7.5 39.0 78-79 30.39402001222674 34.5 30.0 36.0 6.0 39.0 80-81 29.89653704549427 34.0 29.5 36.0 2.0 37.5 82-83 29.43060237237433 34.0 29.0 35.0 2.0 37.0 84-85 28.917740131014604 34.0 29.0 35.0 2.0 36.5 86-87 28.473886887344037 34.0 28.5 35.0 2.0 36.0 88-89 28.03486473664583 34.0 27.5 35.0 2.0 36.0 90-91 27.54420031235376 33.0 26.5 35.0 2.0 35.0 92-93 27.05895858453325 33.0 26.0 35.0 2.0 35.0 94-95 26.529338137040984 33.0 25.0 35.0 2.0 35.0 96-97 25.82679750406954 32.0 23.5 35.0 2.0 35.0 98-99 24.936554652645384 32.0 18.5 34.0 2.0 35.0 100-101 22.40363105205547 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04118767416828106 1101 2 -0.08281038056994916 1101 3 -0.10271338011702369 1101 4 -0.054411021811468174 1101 5 -0.09234660133462569 1101 6 -0.09748709195960714 1101 7 -0.060077271116249165 1101 8 -0.22101193128457908 1101 9 -0.09221059326756631 1101 10-11 -0.24763647574462055 1101 12-13 -0.11348067965651865 1101 14-15 -0.16088052505900663 1101 16-17 -0.1490976460174238 1101 18-19 -0.0027663368060970583 1101 20-21 -0.10399462114633451 1101 22-23 -0.15228624900530008 1101 24-25 -0.1384772389906459 1101 26-27 -0.1387843790248624 1101 28-29 -0.18106812333907385 1101 30-31 -0.05288027592133204 1101 32-33 -0.350106133478171 1101 34-35 -0.20765306196138056 1101 36-37 -0.1188393375005603 1101 38-39 0.1119987771735822 1101 40-41 -0.0028317099703798476 1101 42-43 -0.022635745879718172 1101 44-45 0.1131296165229827 1101 46-47 0.17993359108348272 1101 48-49 0.0531998126695612 1101 50-51 0.18223850549264142 1101 52-53 0.10984918610579086 1101 54-55 0.12208756374104013 1101 56-57 0.1245963155313845 1101 58-59 0.10071104362995698 1101 60-61 0.12948825749209192 1101 62-63 0.0392993188094124 1101 64-65 0.024084231227377018 1101 66-67 0.04655403289952176 1101 68-69 0.07006324465543656 1101 70-71 0.1057910015596022 1101 72-73 0.15513240592032673 1101 74-75 0.08553997062393748 1101 76-77 0.14338319847257353 1101 78-79 -0.09238844568575288 1101 80-81 0.11514113458142461 1101 82-83 0.08994329930962763 1101 84-85 0.11915469689425251 1101 86-87 0.10594002727097163 1101 88-89 -0.07063826654150063 1101 90-91 -0.051232840733622 1101 92-93 -0.009537543938925808 1101 94-95 -0.14405927031559074 1101 96-97 -0.09560804521465371 1101 98-99 0.05232132810388279 1101 100-101 0.18371390646119679 1102 1 -0.1638183161203166 1102 2 -0.2436387395604207 1102 3 -0.36264446154457985 1102 4 -0.27263751962131266 1102 5 -0.22999506425365013 1102 6 -0.34448759029667997 1102 7 -0.21164667177563956 1102 8 -0.2871706913641674 1102 9 -0.25627543486498894 1102 10-11 -0.26614423608059923 1102 12-13 -0.3036608212853551 1102 14-15 -0.29421300074702117 1102 16-17 -0.3416726493577329 1102 18-19 -0.34810489906754327 1102 20-21 -0.4298170127309433 1102 22-23 -0.5059940754251073 1102 24-25 -0.3640481147999921 1102 26-27 -0.4671343416495759 1102 28-29 -0.35789563010092706 1102 30-31 -0.2270926415418586 1102 32-33 -0.32909946551149716 1102 34-35 -0.2847210705269987 1102 36-37 -0.346348717432015 1102 38-39 -0.33332069119465046 1102 40-41 -0.37718757319490237 1102 42-43 -0.26554053641564224 1102 44-45 -0.1865093147967798 1102 46-47 -0.08741747654952547 1102 48-49 -0.2580967755270791 1102 50-51 -0.23921021092829164 1102 52-53 -0.08589368821576926 1102 54-55 -0.0598320992675454 1102 56-57 -0.1413951585713349 1102 58-59 -0.35433683103465796 1102 60-61 -0.21516306003168495 1102 62-63 -0.18483229273323332 1102 64-65 -0.21196549053442482 1102 66-67 -0.3136987558019513 1102 68-69 -0.19314945860650568 1102 70-71 -0.4349717520777219 1102 72-73 -0.38681118990664487 1102 74-75 -0.424855593453902 1102 76-77 -0.21394005436848929 1102 78-79 -0.3276206960387391 1102 80-81 -0.37808075902722393 1102 82-83 -0.26069799369451374 1102 84-85 -0.2224039064619383 1102 86-87 -0.2678671686441092 1102 88-89 -0.5268022086043977 1102 90-91 -0.6546908215295879 1102 92-93 -0.7702910620903154 1102 94-95 -0.5472087696312791 1102 96-97 -0.6701256874302999 1102 98-99 -0.6464372980024784 1102 100-101 -0.17056425409723275 1103 1 0.08517419971157736 1103 2 -0.14447351215685345 1103 3 -0.0836879769198795 1103 4 -0.09179659293439357 1103 5 -0.03101445956463067 1103 6 -0.03291938907776881 1103 7 0.014263041761374495 1103 8 -0.04868479614251697 1103 9 -0.021457179053342657 1103 10-11 -0.03628523423437713 1103 12-13 -0.09640445378688867 1103 14-15 -0.1840833181789776 1103 16-17 -0.09615297612008078 1103 18-19 -0.19442137813370408 1103 20-21 -0.18085303401218056 1103 22-23 -0.16983929012346266 1103 24-25 -0.245506187577071 1103 26-27 -0.2851568934134221 1103 28-29 -0.09169151281587062 1103 30-31 -0.26618476430603266 1103 32-33 -0.169523651087097 1103 34-35 -0.07872778047082107 1103 36-37 -0.03637363157253759 1103 38-39 -0.15822565020046397 1103 40-41 -0.03228057936884454 1103 42-43 0.010910885973338225 1103 44-45 -0.04571836528018025 1103 46-47 0.08306820030117024 1103 48-49 0.2925814151703179 1103 50-51 0.21579544686457552 1103 52-53 0.18366167874669515 1103 54-55 0.4845096115696066 1103 56-57 0.3141688340437554 1103 58-59 0.2281639630415313 1103 60-61 0.2168379623414154 1103 62-63 0.4451728687620147 1103 64-65 0.17143682816805494 1103 66-67 0.34338068159506463 1103 68-69 0.36122739010212257 1103 70-71 0.36185516909036863 1103 72-73 0.2323511093409678 1103 74-75 0.320642987134903 1103 76-77 0.417604870492049 1103 78-79 0.2825226347138461 1103 80-81 0.26967200535051816 1103 82-83 0.2610882310687508 1103 84-85 0.4496327004504934 1103 86-87 0.6347654420798925 1103 88-89 0.6037315098558906 1103 90-91 0.5329928085897215 1103 92-93 0.31642950249785784 1103 94-95 0.08541312106413557 1103 96-97 0.23429235398076997 1103 98-99 0.5689645541802371 1103 100-101 0.8047114392305446 1104 1 -0.01936118051526492 1104 2 0.048940104274848295 1104 3 -0.1298926787647332 1104 4 -0.162159872474966 1104 5 -0.014611298873823841 1104 6 -0.13206299876605954 1104 7 -0.062041410633135285 1104 8 -0.09041011010901201 1104 9 0.026990175809594064 1104 10-11 0.006319254951343112 1104 12-13 -0.0679632669595236 1104 14-15 -0.06321760348086514 1104 16-17 -0.16224712677730935 1104 18-19 -0.1064520723303346 1104 20-21 -0.11351294674565082 1104 22-23 -0.20973342743195644 1104 24-25 -0.16983793714562267 1104 26-27 -0.19310866174252794 1104 28-29 -0.20570235685367777 1104 30-31 -0.2769498933366705 1104 32-33 -0.3043545742506666 1104 34-35 -0.2182029779646797 1104 36-37 -0.2900051611500061 1104 38-39 -0.29671448806168144 1104 40-41 -0.12262914899942245 1104 42-43 -0.11727427477205055 1104 44-45 -0.09473813751658611 1104 46-47 -0.013358810565549106 1104 48-49 0.07581244657042419 1104 50-51 -0.0806265006893625 1104 52-53 -0.16992079587838305 1104 54-55 -0.31741260608248467 1104 56-57 -0.2063030601316953 1104 58-59 -0.2047098253389521 1104 60-61 -0.28967480591963124 1104 62-63 -0.16868443195963323 1104 64-65 6.111761103611002E-4 1104 66-67 -0.17720217803905314 1104 68-69 -0.16506616301843735 1104 70-71 -0.22099345068015808 1104 72-73 -0.08542799015872049 1104 74-75 -0.11942530840606125 1104 76-77 -0.1777567075781512 1104 78-79 -0.05755443942785021 1104 80-81 0.01941678479557396 1104 82-83 0.1979727221088261 1104 84-85 0.18896037142195965 1104 86-87 0.244138423125019 1104 88-89 -0.06451546933553942 1104 90-91 0.08014556643481185 1104 92-93 0.13272248681769838 1104 94-95 0.20258376818510015 1104 96-97 0.3651464394988082 1104 98-99 0.39093502454555207 1104 100-101 0.6018973223199673 1105 1 0.04230134199934099 1105 2 0.009164679242996243 1105 3 -0.04231230710897904 1105 4 -0.042166549977928014 1105 5 0.01773909430809084 1105 6 -0.09088635245406351 1105 7 0.014800130464266203 1105 8 0.013154094960619034 1105 9 0.07721940745744149 1105 10-11 -0.009087501627313088 1105 12-13 -0.0012685430654926222 1105 14-15 0.04601703167267601 1105 16-17 -0.06086259925457682 1105 18-19 0.06910229220861908 1105 20-21 0.09588546295429268 1105 22-23 0.21836814090262635 1105 24-25 0.08836405731907604 1105 26-27 0.1502821039737583 1105 28-29 0.04250165843428988 1105 30-31 0.07153703720850757 1105 32-33 0.13676039302077925 1105 34-35 -0.00440866262839279 1105 36-37 0.037085116932850326 1105 38-39 0.17559074411333597 1105 40-41 0.07209035194509283 1105 42-43 0.12852179780186646 1105 44-45 0.13284715440451578 1105 46-47 0.3050679345910652 1105 48-49 0.43442739187640456 1105 50-51 0.335966918455739 1105 52-53 0.13288217385319712 1105 54-55 0.27486433456682136 1105 56-57 0.16110439934201537 1105 58-59 0.45997690312390205 1105 60-61 0.6877492594496033 1105 62-63 0.6688116801521247 1105 64-65 0.6538526644906284 1105 66-67 0.49896511098260987 1105 68-69 0.43957821601771485 1105 70-71 0.2277280710876184 1105 72-73 0.5200124175204053 1105 74-75 0.5000132541735631 1105 76-77 0.43842024625042697 1105 78-79 0.4439378067611415 1105 80-81 0.5980259312173963 1105 82-83 0.6223941076906918 1105 84-85 0.6834776143161356 1105 86-87 0.7734751537844211 1105 88-89 0.7740722387872907 1105 90-91 0.8286753735779833 1105 92-93 0.7932712721271926 1105 94-95 0.5762891672976949 1105 96-97 0.5229052586351273 1105 98-99 0.5445569548710836 1105 100-101 0.9071853774972318 1106 1 -0.19472464907443054 1106 2 -0.16648667281791063 1106 3 -0.17222987077398244 1106 4 0.05467146524916444 1106 5 -0.08466468621173107 1106 6 -0.13007564098123225 1106 7 -0.3383613292364487 1106 8 -0.2857106989485416 1106 9 -0.3991323909148363 1106 10-11 -0.8480027024674328 1106 12-13 -0.4429057181770659 1106 14-15 -0.4857876414698836 1106 16-17 -0.4663727068943757 1106 18-19 -0.7235445967386127 1106 20-21 -0.39822007655738645 1106 22-23 -0.4013136653457394 1106 24-25 -0.5533886336759366 1106 26-27 -0.7585842651519883 1106 28-29 -0.3834432089849713 1106 30-31 -0.37510092551136154 1106 32-33 -0.1196848682026399 1106 34-35 0.025520452624959944 1106 36-37 -0.4519954816494547 1106 38-39 -0.39550718185148526 1106 40-41 -0.22391909935446108 1106 42-43 -0.24803582887179942 1106 44-45 -0.18010427641722515 1106 46-47 -0.06449468026848137 1106 48-49 0.09648147431273912 1106 50-51 -0.011680233027590248 1106 52-53 0.10133268579858878 1106 54-55 -0.08423254125361268 1106 56-57 -0.12785018191290476 1106 58-59 -0.1475715347863087 1106 60-61 -0.25230639820331646 1106 62-63 -0.18997855350500004 1106 64-65 0.07321522684294735 1106 66-67 0.17073512936013202 1106 68-69 -0.1623277960589391 1106 70-71 0.16338992459247947 1106 72-73 -0.09913171479778526 1106 74-75 0.16692320429170593 1106 76-77 0.26870431030923925 1106 78-79 0.16761226199873747 1106 80-81 -0.18237312365264557 1106 82-83 -0.1949259238136385 1106 84-85 -0.21726483777102956 1106 86-87 -0.12375666559074361 1106 88-89 0.13422030914696492 1106 90-91 0.17036295562051507 1106 92-93 0.353996921904038 1106 94-95 0.20671500084980465 1106 96-97 0.38001946183806723 1106 98-99 0.2402702654512865 1106 100-101 0.5528677088177858 1107 1 -0.033274855388174984 1107 2 0.08263928011382404 1107 3 0.03696780900132879 1107 4 0.1332703150438448 1107 5 0.08918176484510099 1107 6 0.09037344435290606 1107 7 0.2678741507835696 1107 8 0.1388493054105453 1107 9 0.2658928472252242 1107 10-11 0.18294733146412057 1107 12-13 0.24275758175308937 1107 14-15 0.23081529001809997 1107 16-17 0.09480068086153892 1107 18-19 0.13349706724489607 1107 20-21 0.21575774161902217 1107 22-23 0.24060616702744397 1107 24-25 0.27638147386479517 1107 26-27 0.4057073579650563 1107 28-29 0.2928586976215186 1107 30-31 0.3164949472230276 1107 32-33 0.2553380417871054 1107 34-35 0.4955187684311184 1107 36-37 0.41013591519119785 1107 38-39 0.3607112085545481 1107 40-41 0.42847641870851305 1107 42-43 0.3324869647104336 1107 44-45 0.1952245128950807 1107 46-47 0.22967751369120037 1107 48-49 0.26018065747872754 1107 50-51 0.22232686040058525 1107 52-53 0.18286475730747753 1107 54-55 0.30062630844200555 1107 56-57 0.25879960979194294 1107 58-59 0.010786772499812969 1107 60-61 0.31400179936252925 1107 62-63 0.5313341765236785 1107 64-65 0.6288047689899017 1107 66-67 0.3522481298505298 1107 68-69 0.2540774903283136 1107 70-71 0.3955161697813807 1107 72-73 0.2864565394362302 1107 74-75 0.3696213818949019 1107 76-77 0.3209920895015195 1107 78-79 0.4794182131472091 1107 80-81 0.6224961779517955 1107 82-83 0.6049601454265421 1107 84-85 0.5243513443596761 1107 86-87 0.47307457323289626 1107 88-89 0.5477790602676968 1107 90-91 0.7063212371483765 1107 92-93 0.6669055246671078 1107 94-95 0.6513718958898558 1107 96-97 0.5259647651663286 1107 98-99 0.8011055760612145 1107 100-101 0.9769734761909881 1108 1 0.09525153264268837 1108 2 0.173333897907888 1108 3 0.07071607756693155 1108 4 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90-91 -0.39383967658924135 2316 92-93 -0.24905648707013128 2316 94-95 -0.19825263872237286 2316 96-97 0.20025642920368725 2316 98-99 0.619446586978107 2316 100-101 0.9682020999052554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4797.0 3 1153.0 4 925.0 5 1016.0 6 1149.0 7 1356.0 8 1632.0 9 2084.0 10 2636.0 11 3243.0 12 4254.0 13 5708.0 14 6227.0 15 7670.0 16 9929.0 17 13166.0 18 11617.0 19 8158.0 20 7951.0 21 8451.0 22 9145.0 23 10184.0 24 11338.0 25 12769.0 26 14522.0 27 17298.0 28 20312.0 29 24263.0 30 29825.0 31 36671.0 32 46849.0 33 61450.0 34 82788.0 35 116696.0 36 174027.0 37 245559.0 38 199855.0 39 24867.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33648095687756 18.844257201197713 14.915377351637977 22.903884490286757 2 15.02171030918934 19.96565728455312 41.10248508601414 23.9101473202434 3 18.835070686988285 24.423623275993535 30.41255122698669 26.32875481003149 4 12.397722541225683 16.485996662567498 38.07836188226029 33.03791891394653 5 13.532214101163397 36.89044316881599 34.88479805567973 14.69254467434088 6 31.30298777402321 36.100567919985984 18.096447130660334 14.499997175330467 7 28.19199163232595 32.61181514284285 21.302380687201687 17.893812537629515 8 26.142316112949704 34.48781483750023 20.484178535488645 18.88569051406142 9 26.185354044785154 16.573492031470646 20.906435343958037 36.33471857978616 10-11 24.407577009742838 26.50153664378918 28.112066850461314 20.97881949600666 12-13 25.53127610377347 23.871530089706187 28.625756126694352 21.971437679825996 14-15 22.43796685997741 25.166468756165788 26.989020708176632 25.406543675680172 16-17 21.634782033232007 28.629991772098013 27.624283085023404 22.11094310964658 18-19 21.55875292986915 26.997687577193403 30.188677722665012 21.254881770272434 20-21 22.219877568740486 26.005748749622747 30.33187651001512 21.442497171621643 22-23 21.95844847293518 25.9882437574465 30.293123336452137 21.760184433166177 24-25 21.886974996389092 26.15567162456901 30.28558033923962 21.671773039802275 26-27 21.66787441172863 26.400553895018174 30.19942318741406 21.732148505839138 28-29 21.384862071414275 26.5176824898805 30.33404024557938 21.763415193125844 30-31 21.662947824578254 26.218563007137757 30.315408917958177 21.803080250325817 32-33 21.416060461661306 26.40626178937434 30.226069511865937 21.951608237098416 34-35 21.954335460008725 26.34727663552986 30.049112199444515 21.6492757050169 36-37 21.848001726840465 26.199760743832496 30.01502916060625 21.937208368720786 38-39 21.70056255323235 26.415430815393538 30.094076265643903 21.78993036573021 40-41 21.834363645019767 26.298164482448925 30.136677347694484 21.73079452483682 42-43 21.67886030101526 26.400546464182266 29.841850586589597 22.078742648212877 44-45 21.744004138402445 26.529072317533615 29.658618114145824 22.068305429918116 46-47 21.853384025999496 26.713278417175033 29.262182824483236 22.171154732342238 48-49 22.043418836942692 26.84471948932688 28.974985684833914 22.136875988896517 50-51 21.897298595124195 27.016590971912574 28.94730152068777 22.13880891227546 52-53 22.14096004965593 27.060666682540635 28.654023183799186 22.144350084004245 54-55 21.866050506367067 26.90615513309765 28.8113317108819 22.41646264965339 56-57 22.012151797814873 27.21336173244822 28.629349992999742 22.14513647673716 58-59 22.10124244425501 26.874952344797343 28.758770070152494 22.265035140795153 60-61 22.0799522990764 26.672457154291347 29.18123365626127 22.06635689037098 62-63 21.904416095793692 26.820177031137792 29.23575641677358 22.039650456294936 64-65 21.820561304166635 26.87461447504259 29.039040844162994 22.265783376627777 66-67 21.66224515812541 27.021912286307202 28.836142766315202 22.479699789252184 68-69 21.658987848370906 26.869261058305227 29.04779963528975 22.423951458034114 70-71 21.715963822125183 26.865396454994304 28.91885960134622 22.49978012153429 72-73 21.759111886816886 26.724368525334636 28.92524539024604 22.59127419760244 74-75 21.682832098047196 26.912508174835985 28.77255821027877 22.632101516838045 76-77 21.74423516624242 26.835057548479558 28.823543414434184 22.59716387084384 78-79 21.725158408830954 26.71584854885205 28.733838869825362 22.825154172491633 80-81 21.648482569612014 26.99183541237078 28.711938162638972 22.64774385537823 82-83 21.74439799472781 26.91899594378589 28.793915759525486 22.542690301960814 84-85 21.621648881854487 26.74891158886038 28.612373129649527 23.017066399635603 86-87 21.671896200643037 26.6870441641104 28.712824549504933 22.92823508574163 88-89 21.73368750005043 26.383013230418424 28.707998847774746 23.1753004217564 90-91 21.57604176375109 26.377348030855597 29.065572784972282 22.981037420421035 92-93 21.69422187795018 26.193911226227073 29.08716806661664 23.024698829206102 94-95 21.452691117557116 26.24280371644928 29.043817146822338 23.260688019171262 96-97 21.398305939540265 26.226588050555527 29.123371724183006 23.251734285721202 98-99 21.41748758932779 26.430818168320346 28.838230357569376 23.313463884782482 100-101 21.408117851333557 26.279094911053164 29.139973470863133 23.172813766750146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1462.0 1 860.5 2 246.0 3 221.5 4 191.0 5 185.0 6 289.5 7 560.5 8 963.5 9 1263.0 10 1268.5 11 1217.5 12 1287.5 13 1428.0 14 1577.5 15 1691.0 16 1761.5 17 1841.0 18 1958.5 19 2130.0 20 2381.0 21 2693.0 22 3167.0 23 3798.0 24 4536.5 25 5661.0 26 7294.5 27 9068.5 28 10593.5 29 12381.5 30 15035.5 31 17816.5 32 20129.0 33 22441.5 34 25638.5 35 28242.5 36 30508.0 37 33354.0 38 35444.0 39 36448.5 40 37418.5 41 40302.5 42 43524.5 43 45557.5 44 47225.0 45 48469.5 46 48546.5 47 49137.5 48 51848.5 49 51524.5 50 48341.0 51 45587.5 52 42729.5 53 40814.5 54 38765.0 55 35185.0 56 31296.5 57 27284.5 58 23644.5 59 20736.0 60 17428.0 61 14074.5 62 11117.0 63 8421.5 64 6309.0 65 4871.0 66 3553.0 67 2697.5 68 2000.5 69 1339.0 70 947.5 71 691.0 72 532.0 73 394.0 74 276.0 75 217.0 76 159.0 77 91.5 78 53.5 79 33.0 80 29.5 81 21.0 82 10.5 83 7.5 84 4.5 85 4.5 86 4.5 87 2.5 88 1.5 89 0.0 90 0.5 91 1.5 92 1.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2022486571124111 2 0.18267620642411325 3 0.1775213222922159 4 0.18243457123043055 5 0.18034039955184727 6 0.1979797686906836 7 0.2003155755629496 8 0.1908918030093247 9 0.1845287429090139 10-11 0.19951012491734063 12-13 0.20104048114399767 14-15 0.18883790386302182 16-17 0.1985033116103294 18-19 0.19044880515423976 20-21 0.1864618244584754 22-23 0.18400519998936804 24-25 0.18042094461640815 26-27 0.1866229145875972 28-29 0.18920035665354587 30-31 0.18976417210547217 32-33 0.18867681373390005 34-35 0.19648968499630703 36-37 0.18392465492480717 38-39 0.18368301973112447 40-41 0.18299838668235685 42-43 0.1853744660869033 44-45 0.1945566034468455 46-47 0.19652995752858748 48-49 0.19898658199769478 50-51 0.19842276654576854 52-53 0.21066561635902478 54-55 0.19890603693313388 56-57 0.1852939210223424 58-59 0.17474251756486497 60-61 0.17333297893504926 62-63 0.17808513774414217 64-65 0.173937066919256 66-67 0.172769163483123 68-69 0.1772796870985332 70-71 0.1788100433251902 72-73 0.18038067208412772 74-75 0.17876977079290976 76-77 0.18376356479568534 78-79 0.18231375363358923 80-81 0.17925304118027516 82-83 0.17784350255045947 84-85 0.17997794676132323 86-87 0.18247484376271103 88-89 0.1771991420339723 90-91 0.18400519998936804 92-93 0.17808513774414217 94-95 0.17607151113011973 96-97 0.18283729655323505 98-99 0.1840454725216485 100-101 0.1730913437413666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.50432119831101 #Duplication Level Percentage of deduplicated Percentage of total 1 81.78952449939442 51.939882344666714 2 10.803948307364529 13.721948070416513 3 3.1818360341250047 6.0618101253430305 4 1.369878071452924 3.479727082082773 5 0.7103086808584514 2.2553835309591843 6 0.4646417119598885 1.7704053911060322 7 0.30892337047377066 1.3732578260961812 8 0.21980374699066382 1.1166790199589922 9 0.16829555008763616 0.9618745202110517 >10 0.8899575043534043 10.249834192189912 >50 0.06513446937496613 2.814938797501816 >100 0.02660773068536338 2.8983203965716147 >500 5.068101683946084E-4 0.19922289510866503 >1k 5.068101683946084E-4 0.7265681513858018 >5k 1.267025420986521E-4 0.43014765640182684 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5346 0.43059391514255263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3885 0.31291757581908286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2594 0.2089338974709655 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1510 0.12162304748695371 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.027253228044825E-5 0.0 16-17 0.0 0.0 0.0 8.05450645608965E-5 0.0 18-19 0.0 0.0 0.0 8.05450645608965E-5 0.0 20-21 0.0 0.0 0.0 1.61090129121793E-4 0.0 22-23 0.0 0.0 0.0 4.027253228044825E-4 0.0 24-25 0.0 0.0 0.0 0.001127630903852551 0.0 26-27 0.0 0.0 0.0 0.00161090129121793 0.0 28-29 0.0 0.0 0.0 0.002738532195070481 0.0 30-31 0.0 0.0 0.0 0.005275701728738721 0.0 32-33 0.0 0.0 0.0 0.009182137359942201 0.0 34-35 0.0 0.0 0.0 0.014739746814644059 0.0 36-37 0.0 0.0 0.0 0.020861171721272193 0.0 38-39 0.0 0.0 0.0 0.027747774741228845 0.0 40-41 0.0 0.0 0.0 0.03527873827767267 0.0 42-43 0.0 0.0 0.0 0.0528375623519481 0.0 44-45 0.0 0.0 0.0 0.07313491862129401 0.0 46-47 0.0 0.0 0.0 0.09560699163378414 0.0 48-49 0.0 0.0 0.0 0.12323394877817165 0.0 50-51 0.0 0.0 0.0 0.15939868276601415 0.0 52-53 0.0 0.0 0.0 0.22524427304454706 0.0 54-55 0.0 0.0 0.0 0.2802565521396394 0.0 56-57 0.0 0.0 0.0 0.3764676317576302 0.0 58-59 0.0 0.0 0.0 0.5071117264754044 0.0 60-61 0.0 0.0 0.0 0.6268419649451771 0.0 62-63 0.0 0.0 0.0 0.7993292207023368 0.0 64-65 0.0 0.0 0.0 0.9217174463026191 0.0 66-67 0.0 0.0 0.0 1.1333093309040942 0.0 68-69 0.0 0.0 0.0 1.3795758657990351 0.0 70-71 0.0 0.0 0.0 1.5763877310535859 0.0 72-73 0.0 0.0 0.0 1.9073474013343095 0.0 74-75 0.0 0.0 0.0 2.237622438566266 0.0 76-77 0.0 0.0 0.0 2.474626291036704 0.0 78-79 0.0 0.0 0.0 2.7476740598981424 0.0 80-81 0.0 0.0 0.0 3.1798386037996327 0.0 82-83 0.0 0.0 0.0 3.5734623343087337 0.0 84-85 0.0 0.0 0.0 4.075539994249082 0.0 86-87 0.0 0.0 0.0 4.720746233914143 0.0 88-89 0.0 0.0 0.0 5.5828200599094195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3355 0.0 44.46261 1 GTATCAA 5540 0.0 38.93175 1 TATCAAC 6790 0.0 31.758228 2 ATCAACG 6755 0.0 31.711832 3 TCAACGC 6810 0.0 31.459528 4 CAACGCA 6930 0.0 30.985823 5 AACGCAG 7110 0.0 30.268187 6 ACGCAGA 7915 0.0 26.889635 7 CGCAGAG 7905 0.0 26.802376 8 GTACATG 4870 0.0 25.753294 1 GTGTAGC 515 0.0 24.906595 1 GAGTACT 4540 0.0 24.588158 12-13 GCAGAGT 8460 0.0 24.5377 9 TACATGG 5145 0.0 24.002594 2 ACATGGG 5115 0.0 23.02906 3 GTACTTT 5020 0.0 21.95145 14-15 CAGAGTA 8205 0.0 21.131826 10-11 AGAGTAC 7400 0.0 20.220951 10-11 AGTACTT 4930 0.0 20.089685 12-13 TAGCCCT 625 0.0 19.761328 4 >>END_MODULE