FastQCFastQC Report
Thu 26 May 2016
SRR937400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241541
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.42229777349278036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.35608973042372344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26460.21312224082813214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.034.686371
GTATCAA64950.030.298281
CGCGCGT458.9519034E-426.38817232-33
ATCAACG80350.024.2971463
TCAACGC80400.024.2820384
TATCAAC81150.024.1161522
CAACGCA82050.023.8516255
GAGTACT46200.023.5960912-13
AACGCAG84550.023.2016166
GTACATG46850.023.1324711
GTACTTT49550.022.2395614-15
ACGCAGA91850.021.3067597
CGCAGAG91550.021.2728088
TACATGG49800.021.2703592
AGTACTT49000.020.9390512-13
ACATGGG49800.020.5072983
CCCTATA2108.6077307E-720.357412
AGAGTAC77250.020.19929510-11
GCAGAGT97150.019.5087499
GTCCTAA3851.2732926E-1118.5194171
Produced by FastQC (version 0.11.5)