##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937400_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241541 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.249862872027585 33.0 31.0 34.0 31.0 34.0 2 32.45847861649354 34.0 31.0 34.0 31.0 34.0 3 32.5139266443879 34.0 31.0 34.0 31.0 34.0 4 36.13851254207473 37.0 35.0 37.0 35.0 37.0 5 36.05799808463836 37.0 35.0 37.0 35.0 37.0 6 36.09363847025592 37.0 35.0 37.0 35.0 37.0 7 36.03576925772085 37.0 35.0 37.0 35.0 37.0 8 36.012449045178535 37.0 35.0 37.0 35.0 37.0 9 37.72749349397241 39.0 37.0 39.0 35.0 39.0 10-11 37.6520872045305 39.0 37.0 39.0 35.0 39.0 12-13 37.55765657356463 39.0 37.0 39.0 35.0 39.0 14-15 38.78533451573488 40.0 38.0 41.0 35.0 41.0 16-17 38.755514316482504 40.0 38.0 41.0 35.0 41.0 18-19 38.74036298438795 40.0 38.0 41.0 34.5 41.0 20-21 38.642378705173655 40.0 38.0 41.0 34.0 41.0 22-23 38.489306434503575 40.0 38.0 41.0 34.0 41.0 24-25 38.43088871007885 40.0 38.0 41.0 34.0 41.0 26-27 38.38593449592079 40.0 38.0 41.0 34.0 41.0 28-29 38.25225626862101 40.0 38.0 41.0 34.0 41.0 30-31 38.35677355802184 40.0 38.0 41.0 34.0 41.0 32-33 38.32063902843321 40.0 38.0 41.0 34.0 41.0 34-35 38.20823396085993 40.0 38.0 41.0 34.0 41.0 36-37 38.04975067275265 40.0 38.0 41.0 33.5 41.0 38-39 37.92966482782284 40.0 37.0 41.0 33.0 41.0 40-41 37.76065913248132 40.0 37.0 41.0 33.0 41.0 42-43 37.572277516409045 40.0 37.0 41.0 33.0 41.0 44-45 37.29088527885909 40.0 37.0 41.0 32.0 41.0 46-47 36.947550262133916 40.0 36.5 41.0 31.0 41.0 48-49 36.63965144928762 40.0 36.0 41.0 31.0 41.0 50-51 36.40498823639332 39.0 36.0 41.0 30.0 41.0 52-53 36.160313674699424 39.0 35.5 41.0 30.0 41.0 54-55 35.938163943035306 39.0 35.0 40.0 29.0 41.0 56-57 35.679016238690465 39.0 35.0 40.0 28.5 41.0 58-59 35.41510509922749 38.0 35.0 40.0 28.0 41.0 60-61 35.095126137598356 38.0 34.0 40.0 27.5 41.0 62-63 34.737398523286785 38.0 34.0 40.0 26.5 41.0 64-65 34.34518191505556 37.0 34.0 40.0 26.0 41.0 66-67 33.93857029288601 36.5 33.5 39.0 26.0 41.0 68-69 33.51062107493832 36.0 33.0 39.0 25.0 40.0 70-71 33.10670932333286 35.5 32.5 39.0 24.5 40.0 72-73 32.64241696407932 35.0 32.0 38.0 23.5 40.0 74-75 32.1647565404606 35.0 32.0 37.0 22.0 39.0 76-77 30.894392935875658 34.0 30.5 36.0 21.0 38.5 78-79 31.500422861588945 34.5 31.0 36.0 22.0 39.0 80-81 31.305325800758894 34.5 31.0 36.0 20.5 37.5 82-83 30.963118817662888 34.0 31.0 35.5 20.0 37.0 84-85 30.617331203721825 34.0 31.0 35.0 18.5 37.0 86-87 30.217493824207175 34.0 31.0 35.0 17.5 36.0 88-89 29.783233095000487 34.0 30.0 35.0 9.5 36.0 90-91 29.30534956155294 34.0 29.0 35.0 6.0 35.0 92-93 28.97591823387226 34.0 29.0 35.0 2.0 35.0 94-95 28.564111455038535 34.0 29.0 35.0 2.0 35.0 96-97 28.11534979513363 34.0 29.0 35.0 2.0 35.0 98-99 27.40453597585581 33.0 27.5 35.0 2.0 35.0 100-101 24.97997770512613 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.053674904756498165 1101 2 -0.02535922538908153 1101 3 -0.046052017138251244 1101 4 0.026940764725978283 1101 5 -0.06087082157962698 1101 6 -0.10606798731433287 1101 7 -0.057039480997381986 1101 8 -0.16072838434735104 1101 9 -0.006969603144440839 1101 10-11 0.018623891180318708 1101 12-13 -0.021547826009829407 1101 14-15 -0.14049539649032283 1101 16-17 -0.11338317435365042 1101 18-19 -0.07699726442550059 1101 20-21 -0.02423993139442615 1101 22-23 0.006202366174235863 1101 24-25 -0.01767766859006059 1101 26-27 -0.05816740471895798 1101 28-29 -0.2008606919978817 1101 30-31 -0.1477626178176905 1101 32-33 -0.03310093337977804 1101 34-35 0.016428491572042958 1101 36-37 0.007990407704099312 1101 38-39 0.03515763140458006 1101 40-41 0.09414772753293477 1101 42-43 0.1923399413173783 1101 44-45 0.06724994772663706 1101 46-47 0.06423533308949203 1101 48-49 -0.02052878328849772 1101 50-51 0.10757381118420994 1101 52-53 0.1894611540467821 1101 54-55 0.021217101695022222 1101 56-57 0.048165328295581844 1101 58-59 0.0442735753288801 1101 60-61 0.009729479732222046 1101 62-63 0.06007175397900966 1101 64-65 0.2218741983822028 1101 66-67 0.31091959526091273 1101 68-69 0.18281056707074583 1101 70-71 0.3242957984575696 1101 72-73 0.14569213730014496 1101 74-75 0.04502436091140538 1101 76-77 0.1254484179891584 1101 78-79 0.04919059546183746 1101 80-81 0.16161268554273533 1101 82-83 0.04058253105714371 1101 84-85 -0.0031808437123999056 1101 86-87 -0.05779153472949261 1101 88-89 -0.05539606670251018 1101 90-91 -0.04105319782935979 1101 92-93 0.07441276979817246 1101 94-95 0.21688099030285102 1101 96-97 0.09911270335864586 1101 98-99 0.2775879992674213 1101 100-101 0.6436717563615666 1102 1 -0.11758618270219756 1102 2 -0.12103542907172482 1102 3 -0.09409505107211658 1102 4 0.01278735996433511 1102 5 -0.09145309955782466 1102 6 -0.10563510212325156 1102 7 -0.1365720913808488 1102 8 -0.10347469105575868 1102 9 -0.07684151607267609 1102 10-11 -0.10211316018090599 1102 12-13 -0.2826193500184786 1102 14-15 -0.17384200075652245 1102 16-17 -0.19629874413708848 1102 18-19 -0.17064965084617256 1102 20-21 -0.1809716797992067 1102 22-23 -0.15822178367355377 1102 24-25 -0.15902483980495674 1102 26-27 -0.2116854658266334 1102 28-29 -0.35249983276366237 1102 30-31 -0.3040118278812045 1102 32-33 -0.1751890650129866 1102 34-35 -0.34246921752188797 1102 36-37 -0.32082877718979574 1102 38-39 -0.20751807755212326 1102 40-41 -0.256768237670137 1102 42-43 -0.33307795765018966 1102 44-45 -0.4234245135818213 1102 46-47 -0.4003495575471874 1102 48-49 -0.29014380887341673 1102 50-51 -0.3053045458201282 1102 52-53 -0.2775822446120415 1102 54-55 -0.26648453523392135 1102 56-57 -0.4188363165778668 1102 58-59 -0.38412522793991144 1102 60-61 -0.3052254171517248 1102 62-63 -0.385187227824467 1102 64-65 -0.5739418917662462 1102 66-67 -0.5319324726573811 1102 68-69 -0.39574568182334247 1102 70-71 -0.2552573502743769 1102 72-73 -0.2881231475644839 1102 74-75 -0.3027442716588915 1102 76-77 -0.2662212730057476 1102 78-79 -0.45715462573842913 1102 80-81 -0.2900770562969939 1102 82-83 -0.17049323603888666 1102 84-85 -0.31583251151657166 1102 86-87 -0.38559711827136667 1102 88-89 -0.3661593349270227 1102 90-91 -0.4108328461767208 1102 92-93 -0.326451330432576 1102 94-95 -0.3170158611457552 1102 96-97 -0.14830804118582464 1102 98-99 -0.08293855884842927 1102 100-101 0.5081558962426165 1103 1 -0.07197891293466085 1103 2 -0.04947427527901738 1103 3 -0.04742074142210129 1103 4 -0.1442238365099655 1103 5 -0.042659599071448895 1103 6 -0.08942354037390032 1103 7 -0.08912107595591579 1103 8 -0.1540722333899467 1103 9 -0.13920627497129345 1103 10-11 -0.11725271907157975 1103 12-13 -0.08929777893873592 1103 14-15 -0.1356047506423721 1103 16-17 -0.18966696719308374 1103 18-19 -0.1021891164328963 1103 20-21 -0.1477036096028712 1103 22-23 -0.14633049270418752 1103 24-25 -0.041089636422192655 1103 26-27 -0.10296402775465197 1103 28-29 -0.1675235061601157 1103 30-31 -0.2217765530315816 1103 32-33 -0.2514762128898269 1103 34-35 -0.07628867437377806 1103 36-37 -0.07177298341137117 1103 38-39 -0.08661501440322183 1103 40-41 -0.10411205280919944 1103 42-43 -0.18979187185026092 1103 44-45 -0.18421278309128297 1103 46-47 -0.06728794716067199 1103 48-49 -0.17379551712982533 1103 50-51 -0.015934496388887 1103 52-53 -0.06532816008263609 1103 54-55 -0.19102496363803567 1103 56-57 -0.23894704057683214 1103 58-59 -0.028624761420154243 1103 60-61 0.033639153153792734 1103 62-63 0.014755449800453846 1103 64-65 -0.09009265705717695 1103 66-67 0.02642096072955269 1103 68-69 -0.23363254581794735 1103 70-71 -0.06652653217738447 1103 72-73 0.02897753137065706 1103 74-75 -0.02579158867396103 1103 76-77 0.18360427838090843 1103 78-79 0.060987533751770684 1103 80-81 -0.09166967535879422 1103 82-83 -0.11893163557832409 1103 84-85 -0.0062358525921482055 1103 86-87 0.0071132622896463715 1103 88-89 0.114244353556888 1103 90-91 0.1285434069747673 1103 92-93 0.03741299172413193 1103 94-95 0.03403529721288834 1103 96-97 0.003112362601914498 1103 98-99 0.25151888859365457 1103 100-101 0.852873056229118 1104 1 -0.05224284304516402 1104 2 -0.004083648917635685 1104 3 -0.06880271675193228 1104 4 -0.05390222737601391 1104 5 -0.06625430784383468 1104 6 -0.0038686785579571392 1104 7 -0.08770729381580367 1104 8 -0.09825216905957035 1104 9 -0.03554382301933856 1104 10-11 -0.060846921512691665 1104 12-13 -0.08976991641623044 1104 14-15 -0.12645938852180194 1104 16-17 -0.12476121549992314 1104 18-19 -0.18015065301024435 1104 20-21 -0.13064862958816548 1104 22-23 -0.30855623951816114 1104 24-25 -0.26976560972078545 1104 26-27 -0.32381323736824186 1104 28-29 -0.2746413402984942 1104 30-31 -0.14916749700076792 1104 32-33 -0.22754926531451503 1104 34-35 -0.19145931294628582 1104 36-37 -0.3062102833242122 1104 38-39 -0.32312087485897223 1104 40-41 -0.37847045052716055 1104 42-43 -0.30095673956135727 1104 44-45 -0.17092471051361713 1104 46-47 -0.26400997819352057 1104 48-49 -0.09963047877851494 1104 50-51 -0.21716320458327232 1104 52-53 -0.10016720427384485 1104 54-55 -0.07836461033058839 1104 56-57 -0.12338185815126224 1104 58-59 -0.35202561764607054 1104 60-61 -0.09407978827190533 1104 62-63 -0.1673189762944176 1104 64-65 -0.272445729748263 1104 66-67 -0.18704238795481842 1104 68-69 -0.06729762477439039 1104 70-71 0.001628288145695933 1104 72-73 0.12757404024937102 1104 74-75 0.25733538178489823 1104 76-77 0.19485173727190386 1104 78-79 0.09662881711086513 1104 80-81 0.021464952538678972 1104 82-83 -5.942894052175518E-4 1104 84-85 -0.026562258721781973 1104 86-87 -0.07216046138564636 1104 88-89 -0.02427341752021661 1104 90-91 -0.07823472490611394 1104 92-93 0.015571208412218596 1104 94-95 0.2581409731536368 1104 96-97 -0.0435786145418291 1104 98-99 -0.05228530323751457 1104 100-101 0.6705372448315998 1105 1 0.06927470736559371 1105 2 -0.005063133168391687 1105 3 0.03536000345190615 1105 4 8.684091833117691E-4 1105 5 -0.05403518031231869 1105 6 -0.03370200456946293 1105 7 -0.10376985147515683 1105 8 -0.12941220947603682 1105 9 -0.09250081462852933 1105 10-11 -0.00625728042847129 1105 12-13 -0.06492011630200523 1105 14-15 -0.060286739925402344 1105 16-17 -0.1036360562502594 1105 18-19 0.002786393951936361 1105 20-21 -0.037799708092748574 1105 22-23 -0.03312540878407333 1105 24-25 -0.1313708462888954 1105 26-27 -0.04409896420565218 1105 28-29 -0.09736558578457988 1105 30-31 -0.1443043418806198 1105 32-33 -0.14419540158828426 1105 34-35 -0.1290207942976025 1105 36-37 -0.04531286207042484 1105 38-39 -0.1758617262406048 1105 40-41 -0.14921209266572077 1105 42-43 -0.1265934306829095 1105 44-45 -0.1049139035022364 1105 46-47 -0.20286812518644837 1105 48-49 -0.1463517369606464 1105 50-51 -0.1545118844585076 1105 52-53 -0.14777411021770348 1105 54-55 -0.13142264310307183 1105 56-57 -0.08315447160291711 1105 58-59 -0.02542525741235835 1105 60-61 -0.05123042746708251 1105 62-63 -0.04770697436145355 1105 64-65 -0.01815306182224674 1105 66-67 0.025002245880784812 1105 68-69 0.019586177887823908 1105 70-71 0.019496407462135323 1105 72-73 0.03996836437185891 1105 74-75 0.23018219459516587 1105 76-77 0.12972464506085757 1105 78-79 0.007594858493810364 1105 80-81 -0.05061728618205308 1105 82-83 0.023117875072223626 1105 84-85 -0.17409290055282156 1105 86-87 -0.10459977079126048 1105 88-89 0.07969477647881362 1105 90-91 0.23164991244397015 1105 92-93 0.3472343459599969 1105 94-95 0.5200215688571923 1105 96-97 0.3111414135739743 1105 98-99 0.5605583465450259 1105 100-101 0.739125392263837 1106 1 -0.26962579145299514 1106 2 -0.09024874619018419 1106 3 -0.13812595086681512 1106 4 -0.10188611956439075 1106 5 -0.20436125014691697 1106 6 -0.19743841832635667 1106 7 -0.10839678767889183 1106 8 -0.1819383318178609 1106 9 -0.1781883966710538 1106 10-11 -0.2832937729461946 1106 12-13 -0.27487233473203787 1106 14-15 -0.2681586223092296 1106 16-17 -0.009647082766399251 1106 18-19 -0.1647977488676986 1106 20-21 -0.14983961357975772 1106 22-23 -0.1654635552792172 1106 24-25 -0.2017935294078299 1106 26-27 -0.2617014845521979 1106 28-29 -0.27497230670871176 1106 30-31 -0.2815844678979431 1106 32-33 -0.23669001959826375 1106 34-35 -0.1368664230715595 1106 36-37 -0.08801278330960116 1106 38-39 -0.15233324107389024 1106 40-41 -0.1687038227786104 1106 42-43 -0.37001060054376467 1106 44-45 -0.21637938000526447 1106 46-47 -0.2189605378931887 1106 48-49 -0.10713199424602493 1106 50-51 -0.101814588580325 1106 52-53 -0.12389172638992108 1106 54-55 -0.06952188174837914 1106 56-57 -0.2105443377095071 1106 58-59 -0.19743155827872982 1106 60-61 -0.18479304232662486 1106 62-63 -0.049703298856073275 1106 64-65 -0.039353376865570056 1106 66-67 -0.033227210669487306 1106 68-69 0.03669092979121302 1106 70-71 0.11664106485253001 1106 72-73 0.016556445232978945 1106 74-75 0.06984719853320343 1106 76-77 0.1456301320653246 1106 78-79 0.011307118929622106 1106 80-81 -0.06567530665986965 1106 82-83 -0.12964663004722965 1106 84-85 -0.0870306794974276 1106 86-87 -0.07275729245048979 1106 88-89 0.059399685903862576 1106 90-91 0.2915680011811723 1106 92-93 0.41379669828344134 1106 94-95 0.3815117237534871 1106 96-97 0.461796178604164 1106 98-99 0.5990776429482843 1106 100-101 1.3697644051281053 1107 1 -0.028710778846310347 1107 2 0.021334544625510432 1107 3 0.04121196281329986 1107 4 0.06918190700624649 1107 5 0.00455407948449249 1107 6 0.028109316185251032 1107 7 -0.04281774697588503 1107 8 -0.06818144024526873 1107 9 -0.11334841985348731 1107 10-11 -0.03284944298290071 1107 12-13 -0.04644116129475151 1107 14-15 0.10333590158403183 1107 16-17 0.057516338524777666 1107 18-19 -0.011564839720051623 1107 20-21 0.03758490729185837 1107 22-23 0.08226501211577641 1107 24-25 -0.04224167357481434 1107 26-27 -0.18482175085296149 1107 28-29 -0.15089824180780909 1107 30-31 -0.03886167134360363 1107 32-33 -0.1076961272776984 1107 34-35 -0.20414996701168064 1107 36-37 -0.13504290561179033 1107 38-39 -0.15036100055120016 1107 40-41 -0.0671714540590358 1107 42-43 -0.10147877175693054 1107 44-45 -0.025468330207615963 1107 46-47 -0.02930644158702478 1107 48-49 0.0323100917766439 1107 50-51 0.09150262932958952 1107 52-53 0.14370339341074612 1107 54-55 0.3308850492046247 1107 56-57 0.14777731359302493 1107 58-59 0.1800725655194242 1107 60-61 0.18078698907863355 1107 62-63 -0.0028361470755342566 1107 64-65 0.17474679303987273 1107 66-67 0.021858559378614473 1107 68-69 0.041345249005388496 1107 70-71 0.07494488351438378 1107 72-73 0.30470566480727257 1107 74-75 0.3820893063513324 1107 76-77 0.30514409353691363 1107 78-79 0.1868981966650729 1107 80-81 0.14905470220691797 1107 82-83 0.14680437724929263 1107 84-85 0.2939332242657571 1107 86-87 0.4179662669445854 1107 88-89 0.5078021785107474 1107 90-91 0.41973118965732326 1107 92-93 0.4214810803576725 1107 94-95 0.2305237459254066 1107 96-97 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2316 76-77 -0.10196766263144852 2316 78-79 -0.3882579843155547 2316 80-81 -0.3730788246435921 2316 82-83 -0.28617978379065434 2316 84-85 -0.03690226510131822 2316 86-87 -0.39868672731300236 2316 88-89 -0.04219151114660136 2316 90-91 -0.29942701063295374 2316 92-93 -0.19592284467545795 2316 94-95 0.20288444511136916 2316 96-97 0.6294825506977197 2316 98-99 0.5900566743042255 2316 100-101 0.6479682016283839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 23.0 6 60.0 7 119.0 8 288.0 9 521.0 10 915.0 11 1521.0 12 2254.0 13 3271.0 14 4372.0 15 5898.0 16 7892.0 17 11766.0 18 10735.0 19 6652.0 20 5678.0 21 5824.0 22 6051.0 23 6580.0 24 7546.0 25 9059.0 26 11243.0 27 13422.0 28 17003.0 29 20932.0 30 26697.0 31 33442.0 32 42875.0 33 57000.0 34 78893.0 35 114022.0 36 178949.0 37 282152.0 38 244029.0 39 23841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.660523838386446 19.92491262912191 14.992004436248275 22.422559096243376 2 15.619379464713608 21.01154935680739 39.45661077644637 23.912460402032636 3 19.244471185405878 24.88230352441039 30.12224324448407 25.75098204569966 4 13.06956435590931 17.372764975139766 37.148028136001955 32.40964253294897 5 13.69938043488767 37.71244188556571 34.09323762239069 14.494940057155928 6 31.778894132372592 36.78178972744355 17.196532373880522 14.242783766303328 7 28.426689090412637 33.24706956918861 20.559449909427073 17.766791430971672 8 26.502628588181942 35.35308137226237 19.494724700996585 18.649565338559096 9 26.487969386431864 16.924370600729254 20.22389917046638 36.36376084237251 10-11 24.788307434067825 27.18319411118924 27.240944922479404 20.787553532263534 12-13 25.801174395283088 24.369427059420534 28.06353655687923 21.765861988417143 14-15 22.564095748750947 25.82685549651602 26.25479142452807 25.354257330204966 16-17 21.879986242902973 29.370717519598628 26.843293938742256 21.906002298756142 18-19 21.84478804968986 27.660061165922027 29.42581034375828 21.06934044062983 20-21 22.542308308787227 26.623889182878376 29.605023112406275 21.228779395928125 22-23 22.254901408019613 26.814191510320722 29.436196115924613 21.49471096573505 24-25 22.290981274367546 26.944500490723772 29.44145284843301 21.323065386475676 26-27 22.140611942853116 27.11472669975736 29.314297796079458 21.43036356131007 28-29 21.776185800598146 27.298645271094795 29.520951620737122 21.404217307569937 30-31 21.958006923670478 26.916493897085715 29.58967023159984 21.53582894764397 32-33 21.761464180401614 27.122745040236286 29.394357496047252 21.72143328331485 34-35 22.205428576261273 27.05399982763356 29.34905089723175 21.391520698873418 36-37 22.242456948628316 26.864619746443935 29.260354076389 21.63256922853875 38-39 22.143654543492165 27.094908395581598 29.345752915345045 21.415684145581192 40-41 22.171183437981632 27.128617333560474 29.28843584920174 21.411763379256158 42-43 22.044480210342144 27.22171473313324 28.98076858014219 21.753036476382427 44-45 22.141784453897763 27.360184673947145 28.754582516303344 21.743448355851747 46-47 22.25504433603079 27.516086863019424 28.37900641219259 21.849862388757195 48-49 22.48713493956301 27.63593791908604 28.08614455744917 21.79078258390178 50-51 22.38105708953631 27.800048488128866 27.931659123621372 21.887235298713453 52-53 22.58403065222977 28.00837829761563 27.650879028562088 21.75671202159252 54-55 22.21545643679911 27.872780681427358 27.820587479591897 22.091175402181644 56-57 22.29845812582911 28.090332900806338 27.77954976919812 21.831659204166435 58-59 22.395796836350954 27.767588827110824 27.77020654170905 22.066407794829168 60-61 22.352584409214032 27.62425888472471 28.254362924784605 21.768793781276656 62-63 22.251419808125547 27.870847599877894 28.222225444024808 21.655507147971754 64-65 22.12291906707836 27.908151204088792 27.96932520525911 21.999604523573737 66-67 21.98513782468722 28.093635248453335 27.87028378442597 22.050943142433475 68-69 22.001770380519048 27.840200202812472 28.053443261237447 22.104586155431033 70-71 22.03773375184549 27.914583569934457 27.953647926246493 22.094034751973552 72-73 22.101001900058073 27.785792011701588 27.84958370283382 22.263622385406524 74-75 21.99488377749909 28.010150289035963 27.717650887083067 22.277315046381876 76-77 22.025893627355035 27.852563870222568 27.794893603996968 22.326648898425425 78-79 22.002374468503255 27.855141312288517 27.601021633598886 22.541462585609334 80-81 21.965525101466646 28.123316104744024 27.610525951217074 22.300632842572256 82-83 21.93318521042273 28.158495541613153 27.661933418791957 22.24638582917216 84-85 21.827992974834007 28.01065453975779 27.486465359657164 22.674887125751038 86-87 21.817604090400557 27.79171207394681 27.5192281205373 22.87145571511533 88-89 21.863756412393954 27.809713895876175 27.43296435639258 22.893565335337296 90-91 21.808544915581905 27.668634811187008 27.649745456888773 22.873074816342314 92-93 21.797886119138983 27.621650444459476 27.625275068706767 22.95518836769477 94-95 21.530460935240963 27.68337896211241 27.68788948572782 23.09827061691881 96-97 21.529856847256756 27.63009840190537 27.724376399973906 23.115668350863967 98-99 21.363548496222435 27.833094382782676 27.321230085216747 23.48212703577815 100-101 21.092999529969763 28.219777599575323 27.369413557734173 23.31780931272074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 131.5 2 107.0 3 134.5 4 125.0 5 124.0 6 237.5 7 508.5 8 835.5 9 1076.5 10 1138.5 11 1192.0 12 1328.0 13 1455.5 14 1610.5 15 1751.0 16 1844.5 17 1934.0 18 2036.5 19 2151.0 20 2391.0 21 2702.5 22 3158.5 23 3814.5 24 4550.5 25 5581.0 26 7023.5 27 8807.0 28 10485.0 29 12523.0 30 15076.0 31 17384.0 32 19782.0 33 22242.5 34 25484.0 35 28070.5 36 30413.0 37 33533.0 38 35439.5 39 36448.5 40 37843.0 41 40281.5 42 42914.5 43 45334.5 44 47330.5 45 48708.5 46 48554.0 47 49207.5 48 52489.0 49 51832.0 50 48633.0 51 46360.5 52 43056.5 53 40574.5 54 38323.5 55 35087.0 56 31350.0 57 27302.5 58 24024.0 59 21146.0 60 17945.0 61 14463.0 62 11349.5 63 8720.0 64 6333.0 65 4748.0 66 3474.0 67 2810.0 68 2113.5 69 1245.0 70 852.5 71 656.5 72 510.5 73 371.5 74 286.0 75 219.0 76 143.5 77 84.0 78 52.5 79 34.0 80 18.5 81 10.0 82 9.5 83 6.5 84 4.5 85 3.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06870494007044471 2 0.0 3 0.0 4 0.0 5 0.0020136266140224125 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004107798292605722 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.054506456089649E-4 24-25 6.040879842067237E-4 26-27 2.8190772596313777E-4 28-29 0.003060712453314067 30-31 2.4163519368268952E-4 32-33 0.0 34-35 0.0 36-37 8.05450645608965E-5 38-39 5.638154519262755E-4 40-41 1.2081759684134476E-4 42-43 0.010873583715721027 44-45 1.2081759684134476E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.027253228044825E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6245279052403423E-4 84-85 3.6245279052403423E-4 86-87 0.0 88-89 0.0 90-91 0.008135051520650546 92-93 0.002657987130509584 94-95 0.0 96-97 0.0 98-99 8.05450645608965E-5 100-101 0.01059167598975789 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.81466695024878 #Duplication Level Percentage of deduplicated Percentage of total 1 80.17785737824434 48.759896932424766 2 11.549411361118901 14.047472107957306 3 3.5207516464487485 6.42340016379962 4 1.5021254493791978 3.654050356859548 5 0.861015051652432 2.6181171802696954 6 0.5205420033904433 1.8993953141883044 7 0.34115354109846646 1.4522997286560873 8 0.24545419241273075 1.1941771970498 9 0.19842233990666902 1.0860289665221818 >10 0.9766788218255363 10.808741186153814 >50 0.06901953917956245 2.8563007755412815 >100 0.036373918067315894 3.830179427297061 >500 5.310032336251346E-4 0.1856835126139018 >1k 5.310032336251346E-4 0.7609794738689732 >5k 1.3275080840628365E-4 0.4232776767976901 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5243 0.42229777349278036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 0.35608973042372344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2646 0.21312224082813214 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.027253228044825E-5 0.0 16-17 0.0 0.0 0.0 8.05450645608965E-5 0.0 18-19 0.0 0.0 0.0 8.05450645608965E-5 0.0 20-21 0.0 0.0 0.0 1.61090129121793E-4 0.0 22-23 0.0 0.0 0.0 4.027253228044825E-4 0.0 24-25 0.0 0.0 0.0 0.0010470858392916545 0.0 26-27 0.0 0.0 0.0 0.0014900836943765854 0.0 28-29 0.0 0.0 0.0 0.0026982596627900327 0.0 30-31 0.0 0.0 0.0 0.005315974261019169 0.0 32-33 0.0 0.0 0.0 0.009343227489063993 0.0 34-35 0.0 0.0 0.0 0.01498138200832675 0.0 36-37 0.0 0.0 0.0 0.02114307944723533 0.0 38-39 0.0 0.0 0.0 0.02802968246719198 0.0 40-41 0.0 0.0 0.0 0.03560091853591625 0.0 42-43 0.0 0.0 0.0 0.053200015142472135 0.0 44-45 0.0 0.0 0.0 0.07373900660550074 0.0 46-47 0.0 0.0 0.0 0.09653325987623446 0.0 48-49 0.0 0.0 0.0 0.12424076208518284 0.0 50-51 0.0 0.0 0.0 0.16028467847618402 0.0 52-53 0.0 0.0 0.0 0.22649272154524097 0.0 54-55 0.0 0.0 0.0 0.28166609076945504 0.0 56-57 0.0 0.0 0.0 0.3775147175969219 0.0 58-59 0.0 0.0 0.0 0.5080379947178546 0.0 60-61 0.0 0.0 0.0 0.6277279606553469 0.0 62-63 0.0 0.0 0.0 0.8000138537511045 0.0 64-65 0.0 0.0 0.0 0.9227242596096303 0.0 66-67 0.0 0.0 0.0 1.1357659553732016 0.0 68-69 0.0 0.0 0.0 1.3834420288979583 0.0 70-71 0.0 0.0 0.0 1.5821467031696899 0.0 72-73 0.0 0.0 0.0 1.9161670859037276 0.0 74-75 0.0 0.0 0.0 2.251315099541618 0.0 76-77 0.0 0.0 0.0 2.4909769391425653 0.0 78-79 0.0 0.0 0.0 2.766642422602234 0.0 80-81 0.0 0.0 0.0 3.2028744922640495 0.0 82-83 0.0 0.0 0.0 3.600163023210671 0.0 84-85 0.0 0.0 0.0 4.107999655267124 0.0 86-87 0.0 0.0 0.0 4.760011952887581 0.0 88-89 0.0 0.0 0.0 5.634328628696112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4440 0.0 34.68637 1 GTATCAA 6495 0.0 30.29828 1 CGCGCGT 45 8.9519034E-4 26.388172 32-33 ATCAACG 8035 0.0 24.297146 3 TCAACGC 8040 0.0 24.282038 4 TATCAAC 8115 0.0 24.116152 2 CAACGCA 8205 0.0 23.851625 5 GAGTACT 4620 0.0 23.59609 12-13 AACGCAG 8455 0.0 23.201616 6 GTACATG 4685 0.0 23.132471 1 GTACTTT 4955 0.0 22.23956 14-15 ACGCAGA 9185 0.0 21.306759 7 CGCAGAG 9155 0.0 21.272808 8 TACATGG 4980 0.0 21.270359 2 AGTACTT 4900 0.0 20.93905 12-13 ACATGGG 4980 0.0 20.507298 3 CCCTATA 210 8.6077307E-7 20.35741 2 AGAGTAC 7725 0.0 20.199295 10-11 GCAGAGT 9715 0.0 19.508749 9 GTCCTAA 385 1.2732926E-11 18.519417 1 >>END_MODULE