FastQCFastQC Report
Thu 26 May 2016
SRR937399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666172
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.6165629958971822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79130.4749209565399011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52720.3164139116489774No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25020.1501645688440329No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.12039573345368905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.043.696391
GTATCAA95850.037.8811871
ATCAACG119550.030.3217013
TCAACGC120050.030.155844
TATCAAC121050.030.0244642
CAACGCA122150.029.6780825
AACGCAG126000.028.6897936
GTACATG87250.027.7796731
TACATGG87750.027.3956742
ACGCAGA142550.025.1264117
CGCAGAG143350.024.9199128
ACATGGG89800.024.9185733
GTATAGG5150.024.9160331
TACCTGG16400.024.0443382
GTACCTG17150.023.5546761
GAGTACT83950.023.16912-13
GCAGAGT152600.022.9112959
GTACTTT86950.022.12412314-15
CATGGGA56850.021.6445184
CGGGTCG1604.9811773E-520.7865685
Produced by FastQC (version 0.11.5)