##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937399_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1666172 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738588813159748 33.0 31.0 34.0 30.0 34.0 2 31.844039510926844 33.0 31.0 34.0 30.0 34.0 3 31.865850584453465 33.0 31.0 34.0 30.0 34.0 4 35.49808063033108 37.0 35.0 37.0 33.0 37.0 5 35.50844270579508 37.0 35.0 37.0 33.0 37.0 6 35.549154589082036 37.0 35.0 37.0 33.0 37.0 7 35.49190059609692 37.0 35.0 37.0 33.0 37.0 8 35.47484053267009 37.0 35.0 37.0 33.0 37.0 9 37.0698781398319 39.0 37.0 39.0 33.0 39.0 10-11 36.953510802006036 39.0 37.0 39.0 33.0 39.0 12-13 36.80949745884578 39.0 37.0 39.0 33.0 39.0 14-15 37.8959744252094 40.0 38.0 41.0 33.0 41.0 16-17 37.81523366135069 40.0 38.0 41.0 33.0 41.0 18-19 37.76638276240388 40.0 37.5 41.0 33.0 41.0 20-21 37.657137738480785 40.0 37.0 41.0 32.0 41.0 22-23 37.728397188285484 40.0 37.5 41.0 32.5 41.0 24-25 37.69212572291456 40.0 37.0 41.0 32.0 41.0 26-27 37.59551264815397 40.0 37.0 41.0 32.0 41.0 28-29 37.45164514828001 40.0 37.0 41.0 32.0 41.0 30-31 37.17535194445712 39.0 36.5 41.0 31.5 41.0 32-33 36.90689286580257 39.0 36.0 41.0 31.0 41.0 34-35 36.89102865730548 39.0 36.0 41.0 31.0 41.0 36-37 36.747261987357845 39.0 36.0 41.0 30.5 41.0 38-39 36.59178254105818 39.0 35.5 41.0 30.0 41.0 40-41 36.34819064298284 39.0 35.0 41.0 30.0 41.0 42-43 36.04761333163683 39.0 35.0 40.5 29.5 41.0 44-45 35.74928458766562 39.0 35.0 40.0 28.5 41.0 46-47 35.277041025776455 38.5 35.0 40.0 26.5 41.0 48-49 34.874919876219266 38.0 34.5 40.0 24.0 41.0 50-51 33.89534333790269 37.5 33.5 39.5 23.5 40.5 52-53 33.996181966807754 38.0 33.5 39.5 23.0 40.5 54-55 34.28579222313182 38.0 34.0 40.0 23.0 41.0 56-57 34.018875602278754 38.0 34.0 40.0 21.5 41.0 58-59 33.73751869554884 38.0 33.0 40.0 20.0 41.0 60-61 33.3967111438675 37.5 33.0 40.0 18.5 41.0 62-63 33.006993575693265 37.0 32.5 40.0 17.5 41.0 64-65 32.56669059376823 36.0 32.0 39.0 13.0 41.0 66-67 32.125683002715206 36.0 31.0 39.0 8.5 40.5 68-69 31.62073213329716 35.0 31.0 39.0 7.5 40.0 70-71 31.137810202067975 35.0 30.5 38.0 4.0 40.0 72-73 30.63343370312309 35.0 30.0 37.5 2.0 39.5 74-75 30.075728076092986 34.5 29.0 37.0 2.0 39.0 76-77 29.537737400460458 34.0 29.0 36.0 2.0 39.0 78-79 28.962813563065517 34.0 28.5 36.0 2.0 38.5 80-81 28.36959599609164 34.0 27.0 35.0 2.0 37.0 82-83 27.78955774073745 33.5 26.0 35.0 2.0 37.0 84-85 27.194485623333005 33.0 25.5 35.0 2.0 36.0 86-87 26.57410069308571 32.5 24.5 35.0 2.0 36.0 88-89 25.98796312745623 32.0 23.5 35.0 2.0 35.5 90-91 25.386235934825457 32.0 20.0 34.5 2.0 35.0 92-93 24.69121765339953 31.0 17.5 34.0 2.0 35.0 94-95 23.89022471869651 31.0 4.5 34.0 2.0 35.0 96-97 23.00736538604658 31.0 2.0 34.0 2.0 35.0 98-99 21.802603512722577 30.0 2.0 34.0 2.0 35.0 100-101 18.76970204756772 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22029204509194855 1101 2 0.262354906260601 1101 3 0.09127679876993966 1101 4 0.03340808079376956 1101 5 0.03407008242298559 1101 6 0.048461933221872755 1101 7 0.16370197778662998 1101 8 0.1624141719116068 1101 9 0.07497328486304156 1101 10-11 0.21181453140234652 1101 12-13 0.36352532993141295 1101 14-15 0.3424019953677657 1101 16-17 0.43970812575929585 1101 18-19 0.3924965454255016 1101 20-21 0.4209485158823085 1101 22-23 0.31834725658803364 1101 24-25 0.3419429817073407 1101 26-27 0.3157472989361807 1101 28-29 0.3819612604503675 1101 30-31 0.3505466450722423 1101 32-33 0.32422647541492466 1101 34-35 0.39339596601102045 1101 36-37 0.3966071424992208 1101 38-39 0.40061343227580437 1101 40-41 0.7112456170885295 1101 42-43 0.687194611037846 1101 44-45 0.770767528092172 1101 46-47 0.7025912545446573 1101 48-49 0.7392591841472722 1101 50-51 0.8100080609403051 1101 52-53 0.7837096656438973 1101 54-55 0.7912741426938581 1101 56-57 0.9040286726889875 1101 58-59 0.7689510185852697 1101 60-61 0.7587396672179096 1101 62-63 0.909409376299962 1101 64-65 0.6803508633377007 1101 66-67 0.9617822595876113 1101 68-69 0.984398918228468 1101 70-71 0.8225861203180251 1101 72-73 0.8521549097877674 1101 74-75 0.9038455300283879 1101 76-77 0.9562362630183863 1101 78-79 1.169569975338515 1101 80-81 1.3398267573824434 1101 82-83 1.256101568711827 1101 84-85 1.3771750117099373 1101 86-87 1.3374021052459852 1101 88-89 1.4708608643239423 1101 90-91 1.4257292188094866 1101 92-93 1.44656124020608 1101 94-95 1.8598397135615592 1101 96-97 1.8193150429256697 1101 98-99 1.8738556765367314 1101 100-101 2.101324619039694 1102 1 0.17816108652223406 1102 2 0.21762628090905523 1102 3 0.16991716480040608 1102 4 0.17116675771118395 1102 5 0.08804633693944197 1102 6 0.07479701124874794 1102 7 0.11315160823664172 1102 8 0.1237995080906984 1102 9 0.1984020112798177 1102 10-11 0.1591685922545949 1102 12-13 0.3115267894036151 1102 14-15 0.532401381872809 1102 16-17 0.4961319022404851 1102 18-19 0.5252689614009114 1102 20-21 0.36983631411350615 1102 22-23 0.32811716369198507 1102 24-25 0.2946384847893455 1102 26-27 0.4120991027727925 1102 28-29 0.4565695116345765 1102 30-31 0.5357317106677684 1102 32-33 0.5674521995811332 1102 34-35 0.5745279287751046 1102 36-37 0.5979343903931564 1102 38-39 0.5345619826518089 1102 40-41 0.5941859369454221 1102 42-43 0.7125981454145531 1102 44-45 0.8324447587113823 1102 46-47 0.9619575465488737 1102 48-49 0.9058650394722463 1102 50-51 0.6452055578808995 1102 52-53 0.8342075652309191 1102 54-55 1.0155743548339515 1102 56-57 0.7926607726822041 1102 58-59 0.9408660269320279 1102 60-61 0.9185752828600968 1102 62-63 0.9208139245577982 1102 64-65 0.9805064973201567 1102 66-67 0.9315103198780186 1102 68-69 1.1062898711152833 1102 70-71 1.2843008064184893 1102 72-73 1.3926100584634291 1102 74-75 1.3934702002748516 1102 76-77 1.3383193044034662 1102 78-79 1.3624705471803864 1102 80-81 1.530283036180382 1102 82-83 1.5392181876442805 1102 84-85 1.7329794811821024 1102 86-87 1.7549314319191325 1102 88-89 1.4808253139059566 1102 90-91 1.554191025519831 1102 92-93 1.7543597297169313 1102 94-95 1.5136420906930503 1102 96-97 1.6249792157891783 1102 98-99 1.7609879428193693 1102 100-101 1.8472900825028447 1103 1 0.2722973906035513 1103 2 0.21220034194120885 1103 3 0.22517300707748333 1103 4 0.2658229828186762 1103 5 0.20174024917336197 1103 6 0.23101183569451678 1103 7 0.234074002812811 1103 8 0.2538246589254811 1103 9 0.33335800985924635 1103 10-11 0.27740046449196853 1103 12-13 0.25929880365742974 1103 14-15 0.3603496576310974 1103 16-17 0.38638392223273854 1103 18-19 0.3470964399168537 1103 20-21 0.3900860216736035 1103 22-23 0.14666698281062907 1103 24-25 0.28154828789652697 1103 26-27 0.20938407667892278 1103 28-29 0.12114784138639578 1103 30-31 0.30484213156051965 1103 32-33 0.20820006321485351 1103 34-35 0.28754958036331146 1103 36-37 0.3254584025682945 1103 38-39 0.4507052170070267 1103 40-41 0.6241138753394253 1103 42-43 0.38427190487746543 1103 44-45 0.4689230248587748 1103 46-47 0.40810145306441825 1103 48-49 0.5753806125240288 1103 50-51 0.5739076564256322 1103 52-53 0.5454029722691374 1103 54-55 0.7050687648678036 1103 56-57 0.7480367259961582 1103 58-59 0.7872785216901832 1103 60-61 0.8170336781425291 1103 62-63 0.6325209760212047 1103 64-65 0.6394375517290243 1103 66-67 0.8820095995629309 1103 68-69 1.0631289017597716 1103 70-71 0.9642312277927871 1103 72-73 0.8787732118383751 1103 74-75 0.9591760037271477 1103 76-77 0.8593887716712665 1103 78-79 0.989158472171308 1103 80-81 0.8358086204080912 1103 82-83 0.9862094425375076 1103 84-85 1.0159115749314154 1103 86-87 1.2845896283624967 1103 88-89 1.1957351591485654 1103 90-91 1.2035705061706388 1103 92-93 1.4189924843904542 1103 94-95 1.47867320068055 1103 96-97 1.4141034611136512 1103 98-99 1.331947381455148 1103 100-101 1.405143272655998 1104 1 0.21072336584906282 1104 2 0.25894840565422683 1104 3 0.17896006088318117 1104 4 0.14724488623909338 1104 5 0.25026381801758646 1104 6 0.3272013057654135 1104 7 0.30412409178534716 1104 8 0.2972392117546505 1104 9 0.27544251206679604 1104 10-11 0.3049535915510546 1104 12-13 0.3553440139364241 1104 14-15 0.6069835958198482 1104 16-17 0.5176830198815736 1104 18-19 0.6555606181144427 1104 20-21 0.47930983987766496 1104 22-23 0.5035316774829255 1104 24-25 0.44034576786291524 1104 26-27 0.4554868429159171 1104 28-29 0.4901740064136817 1104 30-31 0.5018867046304436 1104 32-33 0.6433073234526105 1104 34-35 0.8200768343670788 1104 36-37 0.7476878254502068 1104 38-39 0.8631101781629198 1104 40-41 0.9228756577887438 1104 42-43 0.837717173945407 1104 44-45 0.7055709796741425 1104 46-47 0.783678985427926 1104 48-49 0.8589105337310627 1104 50-51 0.8395681048787651 1104 52-53 0.8160301497289097 1104 54-55 0.982717571925626 1104 56-57 1.0822985005543515 1104 58-59 1.0481078700008908 1104 60-61 0.9130909717203295 1104 62-63 1.089795171224175 1104 64-65 1.1535306237080007 1104 66-67 1.3220490650964258 1104 68-69 1.1034994496806512 1104 70-71 1.1131958617033568 1104 72-73 1.4056541129269569 1104 74-75 1.390895748133321 1104 76-77 1.3877697279977852 1104 78-79 1.4417312777456068 1104 80-81 1.489768342087796 1104 82-83 1.5508543513751825 1104 84-85 1.5831524202296805 1104 86-87 1.6724884265428024 1104 88-89 1.705453830360529 1104 90-91 1.952889156076541 1104 92-93 2.0714509178425473 1104 94-95 2.0876704742937804 1104 96-97 2.0914557094949764 1104 98-99 2.1803185034560215 1104 100-101 2.330545917764084 1105 1 0.20736766115107486 1105 2 0.0019973030655400237 1105 3 -0.001768606422672292 1105 4 0.1582707731039079 1105 5 0.17404138273474956 1105 6 0.17907091266857833 1105 7 0.17373099494929534 1105 8 0.14958820504324422 1105 9 0.3185872867551893 1105 10-11 0.33012137678595366 1105 12-13 0.3313746946650866 1105 14-15 0.3236662420326937 1105 16-17 0.27846921355367016 1105 18-19 0.2945826698881717 1105 20-21 0.3165581620253093 1105 22-23 0.3371846017398781 1105 24-25 0.3293678196366443 1105 26-27 0.2826680509696118 1105 28-29 0.3044117820608534 1105 30-31 0.446757270306378 1105 32-33 0.5196256360125346 1105 34-35 0.6621190203689906 1105 36-37 0.7244375857570162 1105 38-39 0.5833882222664641 1105 40-41 0.6163080643084058 1105 42-43 0.6610632909156848 1105 44-45 0.7487157543625429 1105 46-47 0.752039483989769 1105 48-49 0.8776066027626968 1105 50-51 0.7667013742939162 1105 52-53 0.865646640620561 1105 54-55 1.1217396236993622 1105 56-57 1.0727107728458165 1105 58-59 1.0831030761370357 1105 60-61 1.22229519032053 1105 62-63 1.064387692796565 1105 64-65 1.0161864242832692 1105 66-67 1.2383961408394732 1105 68-69 1.0651485388465254 1105 70-71 1.0120453343783353 1105 72-73 1.3013875740966618 1105 74-75 1.2398890367239268 1105 76-77 1.117635499809868 1105 78-79 1.2274455634598915 1105 80-81 1.2246676709468574 1105 82-83 1.5137019065046182 1105 84-85 1.2844947021088764 1105 86-87 1.3124692510873857 1105 88-89 1.4520981256625412 1105 90-91 1.6290636527208342 1105 92-93 1.8025151556181918 1105 94-95 1.69145762673865 1105 96-97 1.6063166874432078 1105 98-99 1.9269627987580336 1105 100-101 1.7356274134496061 1106 1 0.10012809542526213 1106 2 -0.3590465954264701 1106 3 0.13133454755180196 1106 4 0.21569572003888027 1106 5 0.07074165263205145 1106 6 0.07829114776188106 1106 7 0.29131311411421734 1106 8 0.27368776223431723 1106 9 0.24872766660655543 1106 10-11 -0.14515032168277742 1106 12-13 -0.13852732208295748 1106 14-15 0.00124204327300248 1106 16-17 0.020669347671017135 1106 18-19 0.3463081837737505 1106 20-21 0.2308746346282433 1106 22-23 0.23290476201565014 1106 24-25 0.35542343954143263 1106 26-27 0.3050274078713855 1106 28-29 0.26484744175748176 1106 30-31 0.36431666711084176 1106 32-33 0.42487505548393756 1106 34-35 0.5273269299672521 1106 36-37 0.484304355136139 1106 38-39 0.6773061114146017 1106 40-41 0.8612609292655335 1106 42-43 0.6670498015924196 1106 44-45 0.7595784281747413 1106 46-47 0.9324264845827699 1106 48-49 0.9689371909393145 1106 50-51 0.821327314362307 1106 52-53 0.7152431801713348 1106 54-55 0.8303901925896824 1106 56-57 0.6442518476688903 1106 58-59 0.41154676070633656 1106 60-61 0.5369621968989193 1106 62-63 0.6015512217454102 1106 64-65 0.7778393928765297 1106 66-67 0.9198408396019424 1106 68-69 0.979287111993127 1106 70-71 0.9119622037912656 1106 72-73 0.8959646888002446 1106 74-75 0.8707512671729951 1106 76-77 1.1735083986842767 1106 78-79 1.1421491474965997 1106 80-81 1.162840959426621 1106 82-83 1.2749713659895399 1106 84-85 1.074054608780937 1106 86-87 1.1176288895247524 1106 88-89 1.3261167690822013 1106 90-91 1.0898448585577079 1106 92-93 1.3310292240845456 1106 94-95 1.3532729770786567 1106 96-97 1.2395298508945665 1106 98-99 1.2921066978042468 1106 100-101 0.9836483512734695 1107 1 0.04781445919776317 1107 2 0.22683498233866217 1107 3 0.09917725023831636 1107 4 0.1329139655688465 1107 5 0.022899548945211734 1107 6 0.05835943372432695 1107 7 0.1288644034610087 1107 8 0.056882826477028914 1107 9 0.10859468867969468 1107 10-11 0.043624424491106595 1107 12-13 0.015520418190789087 1107 14-15 0.20566565584001495 1107 16-17 0.29549339151779463 1107 18-19 0.2590084742885921 1107 20-21 0.2877226983618044 1107 22-23 0.3194617119093337 1107 24-25 0.29659418439752017 1107 26-27 0.24656504002569335 1107 28-29 0.2954817824086007 1107 30-31 0.26816224499577146 1107 32-33 0.3976699879467205 1107 34-35 0.5053196820712387 1107 36-37 0.3702413403708036 1107 38-39 0.5538582693109149 1107 40-41 0.5221762007621393 1107 42-43 0.4956730008496635 1107 44-45 0.4770961735399837 1107 46-47 0.5747862437848994 1107 48-49 0.3498635439298212 1107 50-51 0.24438730417931254 1107 52-53 0.2765552889496732 1107 54-55 0.3972804858000174 1107 56-57 0.32472146341060437 1107 58-59 0.48529260514612815 1107 60-61 0.4877873189451023 1107 62-63 0.45649296902745107 1107 64-65 0.21998351219726686 1107 66-67 0.36757512334808595 1107 68-69 0.3257320489484137 1107 70-71 0.5093550577251946 1107 72-73 0.5865049865030798 1107 74-75 0.48055838927907857 1107 76-77 0.4439657349327426 1107 78-79 0.5385566469724772 1107 80-81 0.6565769203718403 1107 82-83 0.7526077132895459 1107 84-85 0.46139175574550606 1107 86-87 0.25350447729317693 1107 88-89 0.4222008087359761 1107 90-91 0.5187091551823464 1107 92-93 0.48408262490517373 1107 94-95 0.7170364920087238 1107 96-97 0.5000722450962485 1107 98-99 0.6986852116860653 1107 100-101 1.0966361616301725 1108 1 0.22066762212565294 1108 2 0.297366767839069 1108 3 0.2894911439109755 1108 4 0.23975200476557035 1108 5 0.24206606992910906 1108 6 0.2757383341479809 1108 7 0.2817591510702613 1108 8 0.20552946455267573 1108 9 0.38140135710695233 1108 10-11 0.27394162857221005 1108 12-13 0.267541627122192 1108 14-15 0.25194497992156784 1108 16-17 0.2739740628239886 1108 18-19 0.37902212507397337 1108 20-21 0.2038626133326673 1108 22-23 0.12862261635755345 1108 24-25 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1.46037975773584 2316 100-101 2.2464564198790953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6586.0 3 1227.0 4 1215.0 5 1351.0 6 1605.0 7 1911.0 8 2404.0 9 3088.0 10 3862.0 11 5105.0 12 6828.0 13 9118.0 14 10316.0 15 13366.0 16 17200.0 17 22816.0 18 19653.0 19 13370.0 20 13280.0 21 14094.0 22 15725.0 23 16988.0 24 19578.0 25 21810.0 26 25193.0 27 29153.0 28 34292.0 29 41463.0 30 49417.0 31 60332.0 32 74973.0 33 96828.0 34 125904.0 35 169117.0 36 228651.0 37 282156.0 38 188018.0 39 18177.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18178338567162 19.010415099426705 14.970938105205384 21.836863409696285 2 15.062934437729139 20.23954912928963 41.24585039399449 23.451666038986744 3 18.844227756534128 25.618940245314032 30.355024317228533 25.181807680923306 4 12.13839414091123 16.768056078612087 38.14297350920867 32.95057627126801 5 12.96989823696979 37.733057794061466 34.442769951780626 14.854274017188116 6 30.467027894407945 36.61120744251842 18.30575408245688 14.61601058061676 7 27.68510961294292 33.508077297138314 21.078500658297163 17.7283124316216 8 26.451320322574436 34.45108455068615 20.109357256962486 18.98823786977693 9 26.695146429974248 16.544268247129114 20.71156901159542 36.049016311301216 10-11 24.35407739070095 27.185161323117562 27.49725310505155 20.963508181129935 12-13 25.641505319748862 24.61250422948231 28.36320162412121 21.38278882664762 14-15 22.696905147921896 25.486178588925007 27.26127740351086 24.555638859642237 16-17 21.1689511064038 29.0458099859773 27.78680446982143 21.99843443779747 18-19 21.14913227081762 27.00412425444088 31.06748911204044 20.779254362701057 20-21 22.071386536591937 25.978950964268844 31.221423238137852 20.728239261001367 22-23 21.67444520587695 25.99961570879026 31.122656552811623 21.20328253252117 24-25 21.253026221186058 26.177854450901922 31.35109518309484 21.218024144817182 26-27 21.091909904172375 26.437827810969196 31.11915563201158 21.35110665284685 28-29 20.996129431148773 26.507547624295903 31.32818159646906 21.168141348086262 30-31 21.19259592984263 26.264172257173122 31.31244018764613 21.23079162533812 32-33 20.9698281241495 26.414172292059934 31.118090705387775 21.49790887840279 34-35 21.318950786621333 26.36731730759399 31.055348285263534 21.258383620521144 36-37 21.347307063826616 26.28554451420217 31.016593704724553 21.350554717246663 38-39 21.170570894907563 26.456703898856095 31.061195575435175 21.311529630801168 40-41 21.33826360623324 26.365163520751256 30.952205558463753 21.34436731455175 42-43 21.066997361915536 26.464169953622275 30.828932265910975 21.639900418551207 44-45 21.277649448800144 26.70599527190742 30.488409197108297 21.527946082184137 46-47 21.421669913937063 26.88090331353897 29.893963264696094 21.803463507827882 48-49 21.46015816265005 26.948607212979475 29.76265979332459 21.828574831045877 50-51 21.68847468015799 27.164202356630867 29.477103916660475 21.670219046550663 52-53 21.707649541826353 27.304358078660414 29.31126271610077 21.67672966341246 54-55 21.471157058768515 27.29053873818767 29.36272435645863 21.875579846585186 56-57 21.51940715332084 27.642204063763607 29.15078068810241 21.687608094813132 58-59 21.591642278554236 27.233068175873417 29.312186078842505 21.863103466729843 60-61 21.503472482168558 27.182865219465864 29.617616559435984 21.69604573892959 62-63 21.470788361107193 27.240442966592894 29.68784812539595 21.60092054690396 64-65 21.41005975191261 27.08083916050277 29.6820046144327 21.827096473151926 66-67 21.314916824742802 27.391384482350645 29.392329180809334 21.901369512097222 68-69 21.33018192554784 27.387842345022626 29.424120416570325 21.857855312859208 70-71 21.41306730353778 27.2955458210144 29.288393950045144 22.002992925402673 72-73 21.38631967582062 27.249186890543758 29.163124926145876 22.201368507489747 74-75 21.295870325167314 27.512723507051373 29.00992683118975 22.18147933659156 76-77 21.47223619673318 27.40512025413329 28.98755946100119 22.135084088132338 78-79 21.424883421827595 27.38163936260074 28.985481969212596 22.207995246359065 80-81 21.338249475364613 27.589611993830964 28.89257806685805 22.17956046394637 82-83 21.432896926267833 27.543046863395627 28.91026924698283 22.113786963353714 84-85 21.4860886631263 27.30731211036712 28.815125211652425 22.39147401485415 86-87 21.45758883875984 27.270975279623293 28.92246793674961 22.348967944867265 88-89 21.667259998340274 26.96872861450239 28.987730061349694 22.376281325807643 90-91 21.556902971611656 26.906988603289605 29.152414765210448 22.383693659888294 92-93 21.447426619715472 26.773727096333637 29.23033577961845 22.54851050433244 94-95 21.28154021515554 26.83514974865246 29.234609980597575 22.648700055594425 96-97 21.23595427910936 26.893001894061836 29.14527866223125 22.725765164597554 98-99 21.161940141201885 26.987547784710873 28.889945342078843 22.9605667320084 100-101 21.659339489725415 26.317253231684912 29.421336426562565 22.602070852027108 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2458.0 1 1423.0 2 431.0 3 465.0 4 412.0 5 405.5 6 672.0 7 1444.5 8 2548.5 9 3129.0 10 2927.0 11 2644.0 12 2660.0 13 2811.0 14 3015.5 15 3245.0 16 3434.0 17 3552.5 18 3715.5 19 4012.0 20 4372.0 21 4858.0 22 5515.0 23 6444.0 24 7607.5 25 9156.0 26 11411.0 27 14417.0 28 16969.0 29 19847.5 30 23458.5 31 26440.5 32 29232.5 33 32422.5 34 36272.5 35 39883.5 36 44104.0 37 47706.0 38 49520.0 39 50911.5 40 52272.0 41 54000.0 42 55514.5 43 57694.0 44 59809.5 45 60571.0 46 60279.5 47 60789.0 48 63466.0 49 63376.5 50 59817.5 51 56403.0 52 53772.0 53 51702.5 54 49487.0 55 45382.5 56 39870.0 57 35337.5 58 31687.0 59 27853.0 60 23827.0 61 19714.5 62 15359.5 63 11508.5 64 8559.0 65 6443.5 66 4679.0 67 3416.0 68 2515.5 69 1754.0 70 1279.5 71 890.5 72 633.0 73 515.0 74 386.0 75 265.0 76 198.5 77 133.5 78 79.5 79 55.0 80 36.0 81 22.0 82 15.5 83 10.5 84 11.5 85 10.5 86 6.5 87 9.5 88 11.0 89 5.5 90 4.5 91 3.0 92 1.5 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27284097920262734 2 0.23803064749617686 3 0.2386308256290467 4 0.23611007747099338 5 0.23869084344233368 6 0.21060250682402537 7 0.2130032193555047 8 0.2129432015422177 9 0.22236599822827416 10-11 0.23049841192866044 12-13 0.22413652372024018 14-15 0.21537392298034055 16-17 0.21069253354395584 18-19 0.19961924699250735 20-21 0.20619119754743206 22-23 0.20229003968377815 24-25 0.20487080565511845 26-27 0.2006095409117426 28-29 0.20985228415793805 30-31 0.22092557070938654 32-33 0.21291319263557423 34-35 0.2133633262352266 36-37 0.2060411530142146 38-39 0.19649832070158424 40-41 0.19496786646276615 42-43 0.20481078784183146 44-45 0.20075958544496006 46-47 0.20967223071807714 48-49 0.21105264042367775 50-51 0.2203554014831602 52-53 0.22872788643669442 54-55 0.21339333514187012 56-57 0.19685842758130614 58-59 0.1881558446546935 60-61 0.19016644139980748 62-63 0.19490784864947916 64-65 0.1877957377749716 66-67 0.19250713611799983 68-69 0.19274720737114776 70-71 0.19508790208934013 72-73 0.19598816928864488 74-75 0.19835887291348073 76-77 0.2054109659747013 78-79 0.2068513934935889 80-81 0.20108968341803848 82-83 0.19655833851487123 84-85 0.18413465116446562 86-87 0.18791577340154558 88-89 0.1948178219295487 90-91 0.19421764379667886 92-93 0.19868897088655912 94-95 0.19418763489003538 96-97 0.2006695587250296 98-99 0.20259012875021307 100-101 0.19115673531904268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1666172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.73559852322509 #Duplication Level Percentage of deduplicated Percentage of total 1 79.23262395328628 46.537755904618905 2 11.96381161359381 14.054032714870877 3 3.671446566303861 6.469338345536907 4 1.6313407931419408 3.832711115221785 5 0.8710027151776356 2.5579432895656287 6 0.5287425325560305 1.8633605468618573 7 0.37802230943827136 1.554235661999207 8 0.279648067420668 1.3140237292652912 9 0.20922183390354498 1.105989267460635 >10 1.1226387795985118 12.204147618137947 >50 0.07681349365518306 3.0919098845390796 >100 0.03295315525547231 3.226272014622763 >500 0.0011221207855937909 0.43373742535835574 >1k 3.060329415255793E-4 0.3490151267733696 >5k 2.0402196101705286E-4 0.7900024800870178 >10k+ 1.0201098050852643E-4 0.6155248750803134 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10273 0.6165629958971822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7913 0.4749209565399011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5272 0.3164139116489774 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2502 0.1501645688440329 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2006 0.12039573345368905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.0017813286983574E-5 0.0 24-25 0.0 0.0 0.0 1.2003562657396715E-4 0.0 26-27 0.0 0.0 0.0 7.202137594438029E-4 0.0 28-29 0.0 0.0 0.0 0.002250667998261884 0.0 30-31 0.0 0.0 0.0 0.005311576475898047 3.0008906643491787E-5 32-33 0.0 0.0 0.0 0.008042386980455798 6.0017813286983574E-5 34-35 0.0 0.0 0.0 0.01140338452452688 6.0017813286983574E-5 36-37 0.0 0.0 0.0 0.016444880840633502 6.0017813286983574E-5 38-39 0.0 0.0 0.0 0.019895905104635056 6.0017813286983574E-5 40-41 0.0 0.0 0.0 0.02550757064696802 6.0017813286983574E-5 42-43 0.0 0.0 0.0 0.034810331706450474 6.0017813286983574E-5 44-45 0.0 0.0 0.0 0.04846438422923924 6.0017813286983574E-5 46-47 0.0 0.0 0.0 0.07799314836643516 6.0017813286983574E-5 48-49 0.0 0.0 0.0 0.146203393167092 6.0017813286983574E-5 50-51 0.0 0.0 0.0 0.1929572697176522 6.0017813286983574E-5 52-53 0.0 0.0 0.0 0.2413016183203175 6.0017813286983574E-5 54-55 0.0 0.0 0.0 0.3035700996055629 6.0017813286983574E-5 56-57 0.0 0.0 0.0 0.35059405631591456 6.0017813286983574E-5 58-59 0.0 0.0 0.0 0.4105818606962547 6.0017813286983574E-5 60-61 0.0 0.0 0.0 0.5022290615854785 6.0017813286983574E-5 62-63 0.0 0.0 0.0 0.5661480327361161 6.0017813286983574E-5 64-65 0.0 0.0 0.0 0.6414103705979934 6.0017813286983574E-5 66-67 0.0 0.0 0.0 0.7832324633951357 6.0017813286983574E-5 68-69 0.0 0.0 0.0 0.9403290896738152 6.0017813286983574E-5 70-71 0.0 0.0 0.0 1.0844618682825062 9.002671993047536E-5 72-73 0.0 0.0 0.0 1.2665559137952145 1.2003562657396715E-4 74-75 0.0 0.0 0.0 1.432445149720437 1.2003562657396715E-4 76-77 0.0 0.0 0.0 1.5630739203395567 1.2003562657396715E-4 78-79 0.0 0.0 0.0 1.7363153383924348 1.2003562657396715E-4 80-81 0.0 0.0 0.0 1.9893804481170012 1.2003562657396715E-4 82-83 0.0 0.0 0.0 2.22209951913728 1.2003562657396715E-4 84-85 0.0 0.0 0.0 2.47459445963562 1.2003562657396715E-4 86-87 0.0 0.0 0.0 2.9272187985394065 1.2003562657396715E-4 88-89 0.0 0.0 0.0 3.5453122486754065 1.2003562657396715E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5710 0.0 43.69639 1 GTATCAA 9585 0.0 37.881187 1 ATCAACG 11955 0.0 30.321701 3 TCAACGC 12005 0.0 30.15584 4 TATCAAC 12105 0.0 30.024464 2 CAACGCA 12215 0.0 29.678082 5 AACGCAG 12600 0.0 28.689793 6 GTACATG 8725 0.0 27.779673 1 TACATGG 8775 0.0 27.395674 2 ACGCAGA 14255 0.0 25.126411 7 CGCAGAG 14335 0.0 24.919912 8 ACATGGG 8980 0.0 24.918573 3 GTATAGG 515 0.0 24.916033 1 TACCTGG 1640 0.0 24.044338 2 GTACCTG 1715 0.0 23.554676 1 GAGTACT 8395 0.0 23.169 12-13 GCAGAGT 15260 0.0 22.911295 9 GTACTTT 8695 0.0 22.124123 14-15 CATGGGA 5685 0.0 21.644518 4 CGGGTCG 160 4.9811773E-5 20.786568 5 >>END_MODULE