FastQCFastQC Report
Thu 26 May 2016
SRR937399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666172
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100090.6007182931894186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.5124921076575527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.31881462418045675No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.13828104181321016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.036.0395741
GTATCAA106650.033.99671
TATCAAC134100.026.9184062
ATCAACG134150.026.8729693
TCAACGC135150.026.674134
CAACGCA137900.026.1421955
AACGCAG142150.025.4274226
ATTAGCG1401.7810278E-523.748413
GTACATG92750.023.4652231
ACGCAGA157850.022.7198777
CGCAGAG159300.022.5130758
TACATGG95350.022.4657232
ATACCGT1503.0358393E-522.1651826
ACATGGG94600.021.8907133
AGTACTT88950.021.70792212-13
GAGTACT87450.021.37413612-13
GCAGAGT168950.020.8335679
ATACGTC1604.9935894E-520.779865
GTACTTT89600.020.7274714-15
TACCTGG16250.020.4601672
Produced by FastQC (version 0.11.5)