##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937399_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1666172 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.272948411088414 33.0 31.0 34.0 31.0 34.0 2 32.4686689009298 34.0 31.0 34.0 31.0 34.0 3 32.52721327690058 34.0 31.0 34.0 31.0 34.0 4 36.144701147300516 37.0 35.0 37.0 35.0 37.0 5 36.07934414934353 37.0 35.0 37.0 35.0 37.0 6 36.12517135085693 37.0 35.0 37.0 35.0 37.0 7 36.06940039803814 37.0 35.0 37.0 35.0 37.0 8 36.04961792660062 37.0 35.0 37.0 35.0 37.0 9 37.75177652727329 39.0 37.0 39.0 35.0 39.0 10-11 37.668527018819184 39.0 37.0 39.0 35.0 39.0 12-13 37.56018286227352 39.0 37.0 39.0 35.0 39.0 14-15 38.79416380781816 40.0 38.0 41.0 35.0 41.0 16-17 38.75054526183371 40.0 38.0 41.0 35.0 41.0 18-19 38.753260467706816 40.0 38.0 41.0 35.0 41.0 20-21 38.65916964154961 40.0 38.0 41.0 34.0 41.0 22-23 38.60288433607094 40.0 38.0 41.0 34.0 41.0 24-25 38.630879645078664 40.0 38.0 41.0 34.0 41.0 26-27 38.47596676693642 40.0 38.0 41.0 34.0 41.0 28-29 38.328637739681135 40.0 38.0 41.0 34.0 41.0 30-31 38.35428695236746 40.0 38.0 41.0 34.0 41.0 32-33 38.23055872983102 40.0 38.0 41.0 34.0 41.0 34-35 38.09692246658808 40.0 37.0 41.0 34.0 41.0 36-37 37.9247601688181 40.0 37.0 41.0 33.0 41.0 38-39 37.80705083268714 40.0 37.0 41.0 33.0 41.0 40-41 37.60606738079862 40.0 37.0 41.0 33.0 41.0 42-43 37.3894961024432 40.0 37.0 41.0 33.0 41.0 44-45 37.05854647659426 40.0 36.0 41.0 31.5 41.0 46-47 36.63782280580877 40.0 36.0 41.0 31.0 41.0 48-49 36.26007068898049 39.0 36.0 41.0 30.0 41.0 50-51 35.99346736111278 39.0 35.5 41.0 29.5 41.0 52-53 35.74377645285121 39.0 35.0 40.0 28.5 41.0 54-55 35.52462770950419 39.0 35.0 40.0 28.0 41.0 56-57 35.23183140756177 38.0 35.0 40.0 27.5 41.0 58-59 34.96963968905971 38.0 34.5 40.0 27.0 41.0 60-61 34.62933088540679 38.0 34.0 40.0 26.0 41.0 62-63 34.248965592988 37.5 34.0 40.0 26.0 41.0 64-65 33.82845918668661 37.0 33.5 39.5 25.0 41.0 66-67 33.395777266692754 36.0 33.0 39.0 23.5 40.5 68-69 32.94237869799757 36.0 32.0 39.0 22.0 40.0 70-71 32.507755501832946 35.0 32.0 38.0 21.5 40.0 72-73 32.03535019193696 35.0 31.5 37.5 20.0 39.5 74-75 31.553801168186716 35.0 31.0 37.0 18.5 39.0 76-77 30.24158010097397 33.5 29.5 35.5 18.0 38.0 78-79 30.875675500488544 34.0 31.0 36.0 18.0 38.5 80-81 30.69216143351347 34.0 31.0 36.0 17.0 37.5 82-83 30.2951093884665 34.0 30.5 35.0 11.5 37.0 84-85 29.86484618634811 34.0 30.0 35.0 7.0 36.5 86-87 29.489221701000858 34.0 30.0 35.0 4.0 36.0 88-89 29.051137577633042 34.0 29.0 35.0 2.0 36.0 90-91 28.506278163358886 34.0 29.0 35.0 2.0 35.0 92-93 28.01011840314205 33.0 27.5 35.0 2.0 35.0 94-95 27.539352779905073 33.0 27.0 35.0 2.0 35.0 96-97 26.880936662001282 33.0 25.5 35.0 2.0 35.0 98-99 25.924174995138557 32.5 24.5 34.0 2.0 35.0 100-101 23.004216551472478 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1819553325609249 1101 2 0.21090661391679077 1101 3 0.1550221904988831 1101 4 0.11790784790610331 1101 5 0.09147811199544975 1101 6 0.15771230886308274 1101 7 0.19271712243600092 1101 8 0.14741715768975894 1101 9 0.224216677118811 1101 10-11 0.2494578478171121 1101 12-13 0.2742851996967062 1101 14-15 0.4833773956542373 1101 16-17 0.28437207514303253 1101 18-19 0.27879244066974707 1101 20-21 0.3158106232146025 1101 22-23 0.20732276294998542 1101 24-25 0.2616195193780584 1101 26-27 0.29501457200597514 1101 28-29 0.2673259616174235 1101 30-31 0.4183213588841568 1101 32-33 0.4345914495615091 1101 34-35 0.37804691091061926 1101 36-37 0.34869080604175906 1101 38-39 0.35573200828017804 1101 40-41 0.41482758319245505 1101 42-43 0.42829546946840225 1101 44-45 0.47712487283907734 1101 46-47 0.4606538787645391 1101 48-49 0.38175730786363005 1101 50-51 0.4172141043390951 1101 52-53 0.25061220253668637 1101 54-55 0.2727682084745382 1101 56-57 0.4461770105982197 1101 58-59 0.5078404080778611 1101 60-61 0.4219681049318993 1101 62-63 0.4714746609100331 1101 64-65 0.46876834498818454 1101 66-67 0.4609457525357712 1101 68-69 0.5655554784239953 1101 70-71 0.5773820425728431 1101 72-73 0.517049051270412 1101 74-75 0.7364727598323313 1101 76-77 0.6230978128777807 1101 78-79 0.6307247390070678 1101 80-81 0.6361857515860869 1101 82-83 0.46906964816511376 1101 84-85 0.5877676988058269 1101 86-87 0.7573073859917372 1101 88-89 0.846364779294646 1101 90-91 0.987149376237241 1101 92-93 1.010631608265605 1101 94-95 1.1500121593734782 1101 96-97 1.5144021564035022 1101 98-99 1.9806740141531414 1101 100-101 2.5693414365617144 1102 1 0.16924952922157388 1102 2 0.2018511055393546 1102 3 0.2163506976719276 1102 4 0.1611511696906618 1102 5 0.10526076042086174 1102 6 0.1393462073651861 1102 7 0.1526446008748792 1102 8 0.16836639582589186 1102 9 0.20056160335411732 1102 10-11 0.11224243682392654 1102 12-13 0.11678490102153916 1102 14-15 0.26519275950518306 1102 16-17 0.24894274168915587 1102 18-19 0.24522619451619931 1102 20-21 0.249831662862519 1102 22-23 0.1651336838059123 1102 24-25 0.06172752677874627 1102 26-27 0.22503255709482062 1102 28-29 0.19949602241800335 1102 30-31 0.17519169511167831 1102 32-33 0.18383668932502673 1102 34-35 0.33031216029631594 1102 36-37 0.14826684874100948 1102 38-39 0.20021265251445897 1102 40-41 0.11573507138267303 1102 42-43 0.12974783804325796 1102 44-45 0.10904404649366484 1102 46-47 0.20290931223100728 1102 48-49 0.37378025903266376 1102 50-51 0.3083728348565984 1102 52-53 0.3569623596236866 1102 54-55 0.4532527726186615 1102 56-57 0.45292626229581856 1102 58-59 0.395393241294407 1102 60-61 0.5204114422152841 1102 62-63 0.4707513826513292 1102 64-65 0.4790258191326018 1102 66-67 0.44438381053364395 1102 68-69 0.3515934634482676 1102 70-71 0.3794693784225771 1102 72-73 0.5138992390321562 1102 74-75 0.34163063791474 1102 76-77 0.2805052800800176 1102 78-79 0.2469037310025719 1102 80-81 0.41255069713033876 1102 82-83 0.4534182618279665 1102 84-85 0.6064623429948988 1102 86-87 0.6797640458023295 1102 88-89 0.6223099651351802 1102 90-91 0.9108919537555593 1102 92-93 0.9554937494921418 1102 94-95 0.965617372143214 1102 96-97 1.1547423554987581 1102 98-99 1.4400380459128037 1102 100-101 2.190587412519168 1103 1 0.09074973086966764 1103 2 0.1761281820633016 1103 3 0.2051022953067232 1103 4 0.10280765363004463 1103 5 0.07605087986475922 1103 6 0.004455167563264695 1103 7 0.07798815505802992 1103 8 0.06696097881562224 1103 9 0.13034367292571858 1103 10-11 0.1320726711972 1103 12-13 0.08132167220411901 1103 14-15 0.14730925692789754 1103 16-17 0.17261881157777026 1103 18-19 0.2388074396380233 1103 20-21 0.11803983392431405 1103 22-23 0.12012678162228951 1103 24-25 0.10761826962625776 1103 26-27 0.04642200277322672 1103 28-29 0.05401381761175372 1103 30-31 0.1516275850619806 1103 32-33 0.18509636291594944 1103 34-35 0.2574015756773491 1103 36-37 0.2490449997182438 1103 38-39 0.16816314539858723 1103 40-41 0.29572618783093674 1103 42-43 0.39282938212593876 1103 44-45 0.39509190430868557 1103 46-47 0.4260761955941419 1103 48-49 0.42937343550183726 1103 50-51 0.3552963971859242 1103 52-53 0.21086931658334862 1103 54-55 0.20846414697584947 1103 56-57 0.21814743313890972 1103 58-59 0.12798253327884623 1103 60-61 0.2607781056716263 1103 62-63 0.2928608926163605 1103 64-65 0.32798901183394236 1103 66-67 0.4705140431011259 1103 68-69 0.48862249977100447 1103 70-71 0.5067601158526784 1103 72-73 0.3299103988999761 1103 74-75 0.4517782774281329 1103 76-77 0.31889980508337956 1103 78-79 0.4436177197624467 1103 80-81 0.5257659459400124 1103 82-83 0.46644726203463094 1103 84-85 0.540073218348283 1103 86-87 0.6367121770963067 1103 88-89 0.6886536938793668 1103 90-91 0.8845816424149078 1103 92-93 0.8214380193755488 1103 94-95 0.8770727898557418 1103 96-97 1.220534310843231 1103 98-99 1.4920558561549235 1103 100-101 2.0531445461466475 1104 1 0.21645845333454616 1104 2 0.2504671023194476 1104 3 0.23540671552538583 1104 4 0.1747294209283936 1104 5 0.16584014713351536 1104 6 0.07777859752353322 1104 7 0.1393052247643567 1104 8 0.19577486247663956 1104 9 0.20477136843842203 1104 10-11 0.20771313535369273 1104 12-13 0.1939276599965325 1104 14-15 0.1294045206693184 1104 16-17 0.27304458757215144 1104 18-19 0.2825275273085168 1104 20-21 0.287058551693967 1104 22-23 0.24902302899355533 1104 24-25 0.19533562104606972 1104 26-27 0.313890014089111 1104 28-29 0.2617876202308267 1104 30-31 0.30139711463203156 1104 32-33 0.41247618094639904 1104 34-35 0.36547016146779043 1104 36-37 0.4736360417030241 1104 38-39 0.4096693261292259 1104 40-41 0.43739842761093684 1104 42-43 0.4228206229672935 1104 44-45 0.3499684277907278 1104 46-47 0.5176722859318375 1104 48-49 0.7028160215842476 1104 50-51 0.6099575913512183 1104 52-53 0.5047933407459979 1104 54-55 0.5124185421401606 1104 56-57 0.504735168738577 1104 58-59 0.4772120626910805 1104 60-61 0.6333044817867446 1104 62-63 0.7644099024763307 1104 64-65 0.6532336863049366 1104 66-67 0.7797710336593795 1104 68-69 0.8350055492207957 1104 70-71 0.7342652056340526 1104 72-73 0.8499114642030534 1104 74-75 0.7123018442995743 1104 76-77 0.7300739959462348 1104 78-79 0.8026450783237351 1104 80-81 0.9068152398808103 1104 82-83 0.6438377330958716 1104 84-85 0.8320880812990161 1104 86-87 0.7386557633799384 1104 88-89 0.8112552823646197 1104 90-91 0.9983022528730281 1104 92-93 0.9353758826143093 1104 94-95 1.055771462961573 1104 96-97 1.1550941236655383 1104 98-99 1.394763846717126 1104 100-101 2.2615595033964304 1105 1 0.06401358276886526 1105 2 0.10137793645175464 1105 3 0.04844602520899599 1105 4 0.03810430108180185 1105 5 0.0646896198084903 1105 6 0.06675271065871868 1105 7 0.023293719491107368 1105 8 0.08168090274509154 1105 9 0.16066782097917098 1105 10-11 0.16408897427987057 1105 12-13 0.0985297692966256 1105 14-15 0.07787048423595166 1105 16-17 0.16805896916371665 1105 18-19 0.13247958866998033 1105 20-21 0.1959751384628987 1105 22-23 0.1473931152831014 1105 24-25 0.15818317617550548 1105 26-27 0.19668004285230012 1105 28-29 0.12252587526437964 1105 30-31 0.16621015394171224 1105 32-33 0.264106094656853 1105 34-35 0.3618747635770845 1105 36-37 0.4283340282802044 1105 38-39 0.3789885974619196 1105 40-41 0.3283380249263743 1105 42-43 0.20781582910343843 1105 44-45 0.25289268474183046 1105 46-47 0.3400596301312575 1105 48-49 0.44970001007897054 1105 50-51 0.3362279652725988 1105 52-53 0.333892094341401 1105 54-55 0.387538388736516 1105 56-57 0.4126929827366581 1105 58-59 0.2995994068905006 1105 60-61 0.3955288346151278 1105 62-63 0.4401050127352022 1105 64-65 0.4061100046175028 1105 66-67 0.40513057344845294 1105 68-69 0.4757054533435152 1105 70-71 0.4732393187692381 1105 72-73 0.4187034105117746 1105 74-75 0.5035290734462734 1105 76-77 0.4563443698676153 1105 78-79 0.49559809845796465 1105 80-81 0.5254633702739042 1105 82-83 0.4631090657900252 1105 84-85 0.6436892318263396 1105 86-87 0.634149531356929 1105 88-89 0.8160491462188872 1105 90-91 0.716902444627582 1105 92-93 0.8164880590284973 1105 94-95 1.032281530075668 1105 96-97 1.3221602789867255 1105 98-99 1.4067485063527947 1105 100-101 1.770907537912251 1106 1 0.034098763910421326 1106 2 0.12727533113915968 1106 3 0.08247747073906453 1106 4 0.11360239184931942 1106 5 0.09580872377160432 1106 6 0.07478866740864731 1106 7 0.08767558815586085 1106 8 -0.040533252731272285 1106 9 -0.056381154020691326 1106 10-11 -0.22900038001714762 1106 12-13 -0.2296437408647023 1106 14-15 -0.09145266652413397 1106 16-17 -0.09621399843197764 1106 18-19 0.05682493529681665 1106 20-21 0.0928316938638929 1106 22-23 0.1743936528609069 1106 24-25 0.13926210035486974 1106 26-27 0.22176275778643628 1106 28-29 0.17987046263186102 1106 30-31 0.08072586285165073 1106 32-33 0.19935484908355505 1106 34-35 -0.1784823654249621 1106 36-37 0.20938718197758277 1106 38-39 0.3129798632082412 1106 40-41 0.2797282454441117 1106 42-43 0.2625753323150519 1106 44-45 0.38027590012209345 1106 46-47 0.437596747938386 1106 48-49 0.4765082388986812 1106 50-51 0.5104874102928036 1106 52-53 0.4196706677096884 1106 54-55 0.5572370126481943 1106 56-57 0.5487469954501023 1106 58-59 0.6300526348512037 1106 60-61 0.654410506537495 1106 62-63 0.5517008983253433 1106 64-65 0.38142659360325837 1106 66-67 0.41484133442584437 1106 68-69 0.6678670702888496 1106 70-71 0.7796772285337639 1106 72-73 0.8372963976154537 1106 74-75 0.7875156284593245 1106 76-77 0.5951971123456836 1106 78-79 0.4513753971572463 1106 80-81 0.45316996363786544 1106 82-83 0.5297102440275445 1106 84-85 0.5971089470763715 1106 86-87 0.5730712722222613 1106 88-89 0.7413369166011208 1106 90-91 0.8600585804962151 1106 92-93 0.7911509644979375 1106 94-95 0.3960266184434822 1106 96-97 0.8522560454230295 1106 98-99 1.0445859472608028 1106 100-101 1.5532655647246365 1107 1 0.05795692078843473 1107 2 0.09699589779284423 1107 3 0.09035731140514969 1107 4 -0.007527321529337883 1107 5 -0.05460009350354511 1107 6 -0.004102584201163495 1107 7 0.04207374323687674 1107 8 0.034479476011725296 1107 9 -0.026700720344926765 1107 10-11 -0.04651962288156142 1107 12-13 0.0456924477003966 1107 14-15 0.0537089136369957 1107 16-17 0.0561011352947034 1107 18-19 0.10880013671045674 1107 20-21 0.03254387452219021 1107 22-23 -0.005379625763794138 1107 24-25 -0.13074448864151123 1107 26-27 -0.13106152566021478 1107 28-29 -0.0343348145552369 1107 30-31 0.07419976144100104 1107 32-33 -0.1825508683683026 1107 34-35 -0.282524115701996 1107 36-37 -0.16498461711793055 1107 38-39 -0.15003340463446335 1107 40-41 -0.17856190999630428 1107 42-43 -0.16466118270486874 1107 44-45 -0.19507526343476655 1107 46-47 -0.28973410952963974 1107 48-49 -0.29522197164970265 1107 50-51 -0.2338670178122655 1107 52-53 -0.23975009960889082 1107 54-55 -0.062103189214688825 1107 56-57 -0.16429622237613017 1107 58-59 -0.14508139132508546 1107 60-61 -0.006492784965693943 1107 62-63 -0.0388588668667893 1107 64-65 -0.10857759939444378 1107 66-67 0.061145506458551324 1107 68-69 0.11528538170878733 1107 70-71 0.046211116940121144 1107 72-73 0.07449876965414859 1107 74-75 -0.18653570581244594 1107 76-77 -0.1275678701335785 1107 78-79 -0.014442686642805569 1107 80-81 0.016942463562990895 1107 82-83 -0.021896572588705254 1107 84-85 0.07448173452133489 1107 86-87 0.18301167175503963 1107 88-89 0.38226656408981796 1107 90-91 0.549709363688283 1107 92-93 0.595069919842512 1107 94-95 0.5965732056428585 1107 96-97 0.6224138818362199 1107 98-99 0.9036128722959909 1107 100-101 1.4525003426455072 1108 1 0.02712251188231818 1108 2 0.0502618574951299 1108 3 0.043977373224237226 1108 4 0.10293468502452185 1108 5 0.003947269514355867 1108 6 0.07293173617942017 1108 7 0.053813046712065216 1108 8 0.14778322644412611 1108 9 0.0725691300171718 1108 10-11 0.10100389403685739 1108 12-13 0.015498361056963006 1108 14-15 0.013276180020724837 1108 16-17 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76-77 0.32005330602592963 2316 78-79 0.2945176442582955 2316 80-81 0.23581031088008686 2316 82-83 0.23553799952519583 2316 84-85 0.32751090242090797 2316 86-87 0.2956121665957312 2316 88-89 0.26166645958147683 2316 90-91 0.37926822517696124 2316 92-93 0.5955940354564362 2316 94-95 0.8914135785991242 2316 96-97 0.9864176844287798 2316 98-99 1.1522668552598745 2316 100-101 1.8388368217218058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 13.0 5 39.0 6 73.0 7 192.0 8 390.0 9 731.0 10 1361.0 11 2306.0 12 3545.0 13 5038.0 14 6532.0 15 9454.0 16 13498.0 17 19997.0 18 18272.0 19 10142.0 20 8497.0 21 8515.0 22 8884.0 23 9711.0 24 10935.0 25 13051.0 26 15667.0 27 19450.0 28 24099.0 29 30261.0 30 37974.0 31 47461.0 32 61502.0 33 82069.0 34 113684.0 35 163938.0 36 251525.0 37 372214.0 38 273702.0 39 21440.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22838952799606 20.0477233821155 15.265718003327667 21.458169086560776 2 15.63085923902214 21.254828433078938 39.89408056311113 23.220231764787787 3 19.268358848906356 25.965866669227427 30.256960265806892 24.508814216059328 4 12.753065109724565 17.588100148123964 37.204142189401814 32.454692552749655 5 13.12901485274197 38.455199600037695 33.846870372960055 14.568915174260288 6 30.812245074338062 37.23583159481734 17.50983691959774 14.442086411246857 7 27.82197756294068 34.0489457270918 20.56078244022826 17.56829426973926 8 26.891101278859566 35.09127509044684 19.339239886398282 18.678383744295306 9 26.970624881464815 16.722283173645938 20.26507467416329 36.04201727072595 10-11 24.649646014937233 27.81672600427807 26.719210261605646 20.81441771917905 12-13 25.837081639243113 25.123853150437615 27.83082625986894 21.208238950450337 14-15 22.81760226435206 26.09250425526296 26.696883635062886 24.39300984532209 16-17 21.37060279490953 29.693693088108553 27.225040391988344 21.71066372499358 18-19 21.386177417457503 27.479605947045084 30.53934407732215 20.59487255817527 20-21 22.29644102835876 26.481939562571515 30.775320278038937 20.446299131030788 22-23 21.898994154826575 26.55784765097731 30.579577843917843 20.963580350278274 24-25 21.558237887419086 26.74474190966651 30.869709912640992 20.827310290273417 26-27 21.447372330174442 26.986481056403605 30.6695671771369 20.89657943628505 28-29 21.249909219842834 27.087238380590033 30.816067097489785 20.84678530207735 30-31 21.428528558627608 26.841304813617345 30.834987327215906 20.89517930053914 32-33 21.304458728701153 27.004867450500246 30.5394407533445 21.1512330674541 34-35 21.603351874836452 26.91711900091947 30.6045834403651 20.874945683878977 36-37 21.67768393659238 26.86862460778359 30.48691851741597 20.966772938208063 38-39 21.498470746117448 27.050118475163426 30.514496702621337 20.936914076097786 40-41 21.681254996482956 26.950638949640254 30.46777283497742 20.90033321889937 42-43 21.40839296373737 27.14344579753083 30.272837796997827 21.17532344173397 44-45 21.554647419354065 27.344775929495874 29.93802560599986 21.162551045150202 46-47 21.7117140367261 27.550787073603445 29.372357715770043 21.365141173900415 48-49 21.802250908069514 27.67520400054736 29.10347791224435 21.419067179138768 50-51 22.068309874370716 27.89621959797668 28.823464804353932 21.212005723298674 52-53 22.054475768408064 28.102950955843692 28.526106548423574 21.31646672732467 54-55 21.7988599016188 28.1315194349683 28.586664522030137 21.48295614138276 56-57 21.789046989146378 28.449403783042808 28.410302177686336 21.351247050124478 58-59 21.835710838977008 27.96881114314729 28.626246269892903 21.5692317479828 60-61 21.807082342039116 28.03474071104304 28.863706748162855 21.294470198754993 62-63 21.761078688154644 28.102260750990894 28.93875902367823 21.197901537176232 64-65 21.60797324645955 27.906722715301903 28.979511118900092 21.50579291933846 66-67 21.543454097176042 28.215814453729866 28.616553393047056 21.624178056047036 68-69 21.650045733573727 28.170951138297845 28.648212789555938 21.53079033857249 70-71 21.672372360116483 28.173501895362545 28.524306014024965 21.62981973049601 72-73 21.659147421420037 28.168271045734254 28.29553904795972 21.877042484885987 74-75 21.61124421728369 28.517794081283327 28.04203287535741 21.82892882607558 76-77 21.682335317122124 28.263318552946515 28.2970485640138 21.757297565917565 78-79 21.70235125785333 28.25812701209719 28.11141346751716 21.92810826253232 80-81 21.709013235128186 28.532768525698426 27.972502238664436 21.785716000508952 82-83 21.709601564309917 28.626319346331634 27.98697728797731 21.677101801381134 84-85 21.665712882152746 28.352997699213677 27.940554667457302 22.040734751176274 86-87 21.592968793137803 28.261848116520984 28.054936705214107 22.090246385127106 88-89 21.719066218853754 28.0173955630031 28.11462442052801 22.148913797615133 90-91 21.675034445999387 28.085156572860903 28.074202279873482 22.165606701266228 92-93 21.422443091022853 27.87316249011567 28.24965976901805 22.454734649843427 94-95 21.311545266635136 27.95941835536787 28.264368864679035 22.464667513317956 96-97 21.27118328719964 28.103731187416425 28.180974113116775 22.444111412267162 98-99 21.101486225570426 28.080228452490058 27.949479253880316 22.868806068059204 100-101 21.168773950039856 28.247691432248402 28.000357749968785 22.583176867742957 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 617.0 1 430.5 2 289.5 3 374.0 4 355.5 5 346.0 6 616.5 7 1303.0 8 2155.5 9 2774.5 10 2733.0 11 2553.0 12 2821.0 13 3074.0 14 3289.5 15 3478.0 16 3664.0 17 3847.5 18 3956.0 19 4194.5 20 4462.0 21 5011.5 22 5758.0 23 6497.5 24 7516.5 25 9044.0 26 11260.5 27 14225.0 28 16823.5 29 19993.5 30 24163.5 31 26772.5 32 29007.0 33 32202.0 34 36337.5 35 39842.5 36 43906.0 37 47754.0 38 49952.0 39 51736.5 40 52735.0 41 54143.0 42 55462.0 43 57860.0 44 60543.5 45 60748.0 46 59887.0 47 60520.5 48 63886.0 49 63562.0 50 59670.0 51 56744.0 52 53987.0 53 51781.5 54 49638.5 55 45815.5 56 40012.5 57 35032.5 58 31799.0 59 28286.5 60 23585.0 61 18942.5 62 14984.0 63 11465.5 64 8230.0 65 6052.0 66 4429.0 67 3334.0 68 2455.5 69 1581.0 70 1111.5 71 817.0 72 635.0 73 504.0 74 373.5 75 268.0 76 185.0 77 113.5 78 60.5 79 34.0 80 23.5 81 13.5 82 8.0 83 8.5 84 5.0 85 1.5 86 2.0 87 2.0 88 1.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04447319964565483 2 0.0 3 0.0 4 0.0 5 9.002671993047536E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005161531942680588 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5004453321745893E-4 22-23 6.601959461568194E-4 24-25 9.002671993047536E-5 26-27 0.0017105076786790318 28-29 0.0035410509839320314 30-31 1.8005343986095073E-4 32-33 1.2003562657396716E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00873259183325611 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.8005343986095073E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6010687972190146E-4 84-85 1.5004453321745893E-4 86-87 0.0 88-89 0.0 90-91 0.009512823405986897 92-93 0.0014704364255310977 94-95 0.0 96-97 0.0 98-99 3.0008906643491786E-4 100-101 0.012243633910544649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1666172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.780393452365544 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90216549110612 44.233156073762984 2 12.529452700639283 14.228545081702048 3 3.985717898759954 6.789318914451775 4 1.7729123970347582 4.026666538408405 5 0.9791641487694446 2.779866281078983 6 0.5764289191588096 1.9637916496295413 7 0.40002422441251206 1.5899472996833863 8 0.2935000078831346 1.3332036740701416 9 0.2178497969937785 1.1132637468142232 >10 1.21788914449003 12.73281545240455 >50 0.08675142920791265 3.389648397878335 >100 0.036342746850688015 3.616707982702706 >500 9.535207201012284E-4 0.3525199005362864 >1k 5.297337333895713E-4 0.4152034732937625 >5k 2.1189349335582855E-4 0.8332343246293691 >10k+ 1.0594674667791427E-4 0.6021112089535308 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10009 0.6007182931894186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.5124921076575527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5312 0.31881462418045675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2304 0.13828104181321016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 6.0017813286983574E-5 16-17 0.0 0.0 0.0 3.0008906643491787E-5 6.0017813286983574E-5 18-19 0.0 0.0 0.0 6.0017813286983574E-5 6.0017813286983574E-5 20-21 0.0 0.0 0.0 6.0017813286983574E-5 6.0017813286983574E-5 22-23 0.0 0.0 0.0 2.400712531479343E-4 6.0017813286983574E-5 24-25 0.0 0.0 0.0 3.0008906643491786E-4 6.0017813286983574E-5 26-27 0.0 0.0 0.0 7.802315727307866E-4 6.0017813286983574E-5 28-29 0.0 0.0 0.0 0.0024007125314793433 6.0017813286983574E-5 30-31 0.0 0.0 0.0 0.005461621009115505 6.0017813286983574E-5 32-33 0.0 0.0 0.0 0.008132413700386275 6.0017813286983574E-5 34-35 0.0 0.0 0.0 0.01143339343117037 6.0017813286983574E-5 36-37 0.0 0.0 0.0 0.016384863027346517 6.0017813286983574E-5 38-39 0.0 0.0 0.0 0.019835887291348074 6.0017813286983574E-5 40-41 0.0 0.0 0.0 0.025597597366898497 6.0017813286983574E-5 42-43 0.0 0.0 0.0 0.03499038514631142 6.0017813286983574E-5 44-45 0.0 0.0 0.0 0.04891451782889161 6.0017813286983574E-5 46-47 0.0 0.0 0.0 0.07901345119231387 6.0017813286983574E-5 48-49 0.0 0.0 0.0 0.148033936472345 6.0017813286983574E-5 50-51 0.0 0.0 0.0 0.19508790208934013 6.0017813286983574E-5 52-53 0.0 0.0 0.0 0.2440324288248752 9.002671993047536E-5 54-55 0.0 0.0 0.0 0.306931097149634 1.2003562657396715E-4 56-57 0.0 0.0 0.0 0.35437517855299455 1.2003562657396715E-4 58-59 0.0 0.0 0.0 0.41454303637319556 1.2003562657396715E-4 60-61 0.0 0.0 0.0 0.5066703797687153 1.2003562657396715E-4 62-63 0.0 0.0 0.0 0.5707093865459268 1.2003562657396715E-4 64-65 0.0 0.0 0.0 0.6463618401941696 1.2003562657396715E-4 66-67 0.0 0.0 0.0 0.7896243605101994 1.2003562657396715E-4 68-69 0.0 0.0 0.0 0.9478613252413317 1.2003562657396715E-4 70-71 0.0 0.0 0.0 1.0925342641696054 1.2003562657396715E-4 72-73 0.0 0.0 0.0 1.2765188708008537 1.2003562657396715E-4 74-75 0.0 0.0 0.0 1.444898845977486 1.2003562657396715E-4 76-77 0.0 0.0 0.0 1.5766979639557022 1.2003562657396715E-4 78-79 0.0 0.0 0.0 1.7516498896872592 1.2003562657396715E-4 80-81 0.0 0.0 0.0 2.0094864155681407 1.2003562657396715E-4 82-83 0.0 0.0 0.0 2.2453264128793426 1.2003562657396715E-4 84-85 0.0 0.0 0.0 2.501902564681197 1.2003562657396715E-4 86-87 0.0 0.0 0.0 2.9625992994720836 1.2003562657396715E-4 88-89 0.0 0.0 0.0 3.600828725965867 1.2003562657396715E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6685 0.0 36.039574 1 GTATCAA 10665 0.0 33.9967 1 TATCAAC 13410 0.0 26.918406 2 ATCAACG 13415 0.0 26.872969 3 TCAACGC 13515 0.0 26.67413 4 CAACGCA 13790 0.0 26.142195 5 AACGCAG 14215 0.0 25.427422 6 ATTAGCG 140 1.7810278E-5 23.74841 3 GTACATG 9275 0.0 23.465223 1 ACGCAGA 15785 0.0 22.719877 7 CGCAGAG 15930 0.0 22.513075 8 TACATGG 9535 0.0 22.465723 2 ATACCGT 150 3.0358393E-5 22.165182 6 ACATGGG 9460 0.0 21.890713 3 AGTACTT 8895 0.0 21.707922 12-13 GAGTACT 8745 0.0 21.374136 12-13 GCAGAGT 16895 0.0 20.833567 9 ATACGTC 160 4.9935894E-5 20.77986 5 GTACTTT 8960 0.0 20.72747 14-15 TACCTGG 1625 0.0 20.460167 2 >>END_MODULE