FastQCFastQC Report
Thu 26 May 2016
SRR937398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667047
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102590.6153995658190801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78990.47383187156690837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.3146282018443391No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21030.12615121229335466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.12567132180436424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.044.321791
GTATCAA93650.039.900241
TATCAAC117200.031.7505512
ATCAACG118200.031.3211023
TCAACGC119500.030.8657074
CAACGCA121950.030.169475
AACGCAG124800.029.5787546
GTACATG88250.026.0191541
ACGCAGA143300.025.5612227
CGCAGAG143250.025.4027928
TACATGG90800.024.8613572
GTACCGT1000.00165218723.7543666
CCGATCG1601.857903E-623.753659
GCAGAGT151700.023.6127269
ACATGGG93100.023.430423
GAGTACT82300.022.3669212-13
AGTACTT85400.022.2781412-13
TACCTGG14250.021.6778182
GTGTAGC10800.021.1289651
GTACTTT87850.020.735714-15
Produced by FastQC (version 0.11.5)