##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937398_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1667047 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750393960098304 33.0 31.0 34.0 30.0 34.0 2 31.852411479700333 33.0 31.0 34.0 30.0 34.0 3 31.87394836498311 33.0 31.0 34.0 30.0 34.0 4 35.50255571678543 37.0 35.0 37.0 33.0 37.0 5 35.51335685196638 37.0 35.0 37.0 33.0 37.0 6 35.558582931375064 37.0 35.0 37.0 33.0 37.0 7 35.50564321221897 37.0 35.0 37.0 33.0 37.0 8 35.49824809978363 37.0 35.0 37.0 33.0 37.0 9 37.10529037273694 39.0 37.0 39.0 33.0 39.0 10-11 36.990239327385495 39.0 37.0 39.0 33.0 39.0 12-13 36.864836144391845 39.0 37.0 39.0 33.0 39.0 14-15 38.01538558900859 40.0 38.0 41.0 33.0 41.0 16-17 37.97850000629856 40.0 38.0 41.0 33.0 41.0 18-19 37.984523231798505 40.0 38.0 41.0 33.0 41.0 20-21 37.941064349115535 40.0 38.0 41.0 33.0 41.0 22-23 37.79783443418212 40.0 38.0 41.0 33.0 41.0 24-25 37.64550999461923 40.0 37.0 41.0 32.0 41.0 26-27 37.47827925667363 40.0 37.0 41.0 32.0 41.0 28-29 37.51758708662683 40.0 37.0 41.0 32.0 41.0 30-31 37.342366771902654 39.5 37.0 41.0 32.0 41.0 32-33 37.12324097640918 39.0 36.0 41.0 31.5 41.0 34-35 36.993252739724795 39.0 36.0 41.0 31.0 41.0 36-37 37.00014876605159 40.0 36.0 41.0 31.0 41.0 38-39 36.90582449085119 40.0 36.0 41.0 31.0 41.0 40-41 36.71337490784603 39.5 36.0 41.0 30.5 41.0 42-43 36.45551115235503 39.0 35.0 41.0 30.0 41.0 44-45 36.19044274096652 39.0 35.0 41.0 29.5 41.0 46-47 35.77490946565994 39.0 35.0 41.0 28.0 41.0 48-49 35.402134732853966 39.0 35.0 41.0 26.5 41.0 50-51 34.50828740881331 38.0 34.0 40.0 25.0 40.5 52-53 34.59229283877419 38.0 34.5 40.0 24.5 40.5 54-55 34.91064948978644 39.0 35.0 40.0 25.0 41.0 56-57 34.722676685180446 38.5 34.5 40.0 24.0 41.0 58-59 34.491545529310216 38.0 34.0 40.0 23.0 41.0 60-61 34.21480978040811 38.0 34.0 40.0 23.0 41.0 62-63 33.88663786923824 38.0 34.0 40.0 22.0 41.0 64-65 33.5294964689058 37.0 33.0 40.0 21.0 41.0 66-67 33.16075971463312 36.5 33.0 39.5 19.5 41.0 68-69 32.697878344161865 36.0 32.5 39.0 18.0 41.0 70-71 32.26578104876467 35.5 32.0 39.0 14.5 40.0 72-73 31.87921246371578 35.0 31.5 38.0 12.0 40.0 74-75 31.426413292486657 35.0 31.0 37.5 8.0 39.5 76-77 30.99868480012861 35.0 31.0 37.0 7.0 39.0 78-79 30.52717319907597 35.0 30.5 36.0 2.0 39.0 80-81 30.04690809557259 34.0 30.0 36.0 2.0 37.5 82-83 29.586254616696472 34.0 29.5 35.5 2.0 37.0 84-85 29.156937386888313 34.0 29.0 35.0 2.0 37.0 86-87 28.729483931766772 34.0 29.0 35.0 2.0 36.0 88-89 28.334239526540046 34.0 29.0 35.0 2.0 36.0 90-91 27.923207024157087 34.0 27.5 35.0 2.0 35.5 92-93 27.491955835678297 33.5 26.5 35.0 2.0 35.0 94-95 26.9953801542488 33.0 25.5 35.0 2.0 35.0 96-97 26.396996605374653 33.0 25.0 35.0 2.0 35.0 98-99 25.665983322605783 32.5 23.5 35.0 2.0 35.0 100-101 23.799386579982446 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08725267224188826 1101 2 0.03807432042868797 1101 3 0.03650280410767692 1101 4 8.772609187630565E-4 1101 5 0.01163704096499174 1101 6 0.04248641326010727 1101 7 0.06913470095189211 1101 8 0.10483381761596178 1101 9 0.21280077579161372 1101 10-11 0.1280316702695643 1101 12-13 0.0404203444438096 1101 14-15 0.19663410263185455 1101 16-17 0.39606950776558136 1101 18-19 0.1770076380802692 1101 20-21 0.22937411990904621 1101 22-23 0.29850770108707536 1101 24-25 0.32483131604468696 1101 26-27 0.33786032396056953 1101 28-29 0.268528432107054 1101 30-31 0.08247815455489871 1101 32-33 0.23719173663404547 1101 34-35 0.07077199748229646 1101 36-37 0.15867571416933401 1101 38-39 0.1908052301019012 1101 40-41 0.14953556484733355 1101 42-43 0.13866521492926864 1101 44-45 0.11450956209795748 1101 46-47 0.14068788945156996 1101 48-49 0.3084699797894572 1101 50-51 0.098261949280527 1101 52-53 0.11542034859655814 1101 54-55 0.34574249366862375 1101 56-57 0.26638149715849124 1101 58-59 0.32410529868409554 1101 60-61 0.23417027466926044 1101 62-63 0.3767172475842173 1101 64-65 0.33012833442990086 1101 66-67 0.37917492748051984 1101 68-69 0.39703244416782724 1101 70-71 0.3914062934035769 1101 72-73 0.3359835525263257 1101 74-75 0.4314017216970072 1101 76-77 0.3693778255136877 1101 78-79 0.30054893989112585 1101 80-81 0.3604080067207178 1101 82-83 0.38784306246527933 1101 84-85 0.34881533324278635 1101 86-87 0.4052868169553143 1101 88-89 0.46143529087034096 1101 90-91 0.610162566633278 1101 92-93 0.6820947562272828 1101 94-95 0.7340767338604195 1101 96-97 0.8295857058550098 1101 98-99 0.9373936661214337 1101 100-101 1.22439131343247 1102 1 0.09808635309076763 1102 2 0.0696266562700032 1102 3 0.1553151126430734 1102 4 0.008478080869345206 1102 5 0.06253586433676617 1102 6 -0.10685758761945863 1102 7 0.017098202585820843 1102 8 0.06443841006075246 1102 9 0.10889140841879197 1102 10-11 0.08628202576615962 1102 12-13 0.09790320952946985 1102 14-15 0.3675380968631714 1102 16-17 0.3228107968642959 1102 18-19 0.24912276872821337 1102 20-21 0.32286736193061216 1102 22-23 0.3320833934089009 1102 24-25 0.42622643885904665 1102 26-27 0.323236003655893 1102 28-29 0.2760766415680962 1102 30-31 0.27306652450155156 1102 32-33 0.31605936993077677 1102 34-35 0.23560237911664217 1102 36-37 0.36276522415308676 1102 38-39 0.47213638715043516 1102 40-41 0.3904785080198465 1102 42-43 0.38594364209465937 1102 44-45 0.3695015459772435 1102 46-47 0.4775660603072467 1102 48-49 0.444533482582969 1102 50-51 0.3604530881279828 1102 52-53 0.4573388190872194 1102 54-55 0.38488383894246425 1102 56-57 0.369355149395858 1102 58-59 0.30129854409849344 1102 60-61 0.3942280259552078 1102 62-63 0.2581667030763768 1102 64-65 0.3890410237793489 1102 66-67 0.2475923352393039 1102 68-69 0.2545591584859963 1102 70-71 0.3644864722191201 1102 72-73 0.2496167771026876 1102 74-75 0.37041381695592435 1102 76-77 0.47170962977861564 1102 78-79 0.45118995039046084 1102 80-81 0.30499026440217136 1102 82-83 0.2787104397958693 1102 84-85 0.3129943540692395 1102 86-87 0.2548761115581648 1102 88-89 0.1398651619467266 1102 90-91 0.3087233583760529 1102 92-93 0.42082698515549666 1102 94-95 0.28470917636574455 1102 96-97 0.292898049392484 1102 98-99 0.48417942289433924 1102 100-101 1.022258484186363 1103 1 0.18080109542739464 1103 2 0.0029737339882061065 1103 3 0.1605178194625303 1103 4 0.05258204327657978 1103 5 0.09634862489646423 1103 6 0.05911642650008986 1103 7 0.03560208657361841 1103 8 0.13334431730594076 1103 9 0.03568232462443888 1103 10-11 0.21334770226755495 1103 12-13 0.10277283609513432 1103 14-15 0.2870298004332099 1103 16-17 0.2779731136280077 1103 18-19 0.05466741396008956 1103 20-21 0.00624349845597294 1103 22-23 -0.007317741109879705 1103 24-25 -0.011309041551044174 1103 26-27 0.35076306881529007 1103 28-29 0.22224657837165296 1103 30-31 0.14580277597612934 1103 32-33 0.16114681277148435 1103 34-35 0.1509595259082417 1103 36-37 0.25890533773986135 1103 38-39 0.20124220549928395 1103 40-41 0.29703100486272405 1103 42-43 0.37026649994346883 1103 44-45 0.3262424810345834 1103 46-47 0.3506538277972169 1103 48-49 0.5652595053574316 1103 50-51 0.42567465338260035 1103 52-53 0.39170840709516597 1103 54-55 0.2715217787955666 1103 56-57 0.3227967576058006 1103 58-59 0.4940557853621357 1103 60-61 0.40249545331437986 1103 62-63 0.18056777217069708 1103 64-65 0.22440607546580793 1103 66-67 0.4374552743448561 1103 68-69 0.5213581279718866 1103 70-71 0.4902100477586373 1103 72-73 0.45342063678675615 1103 74-75 0.4057938420780225 1103 76-77 0.47890856985721797 1103 78-79 0.5296215257240391 1103 80-81 0.47645127827463085 1103 82-83 0.35571448015040374 1103 84-85 0.5031395626710058 1103 86-87 0.5648842345345884 1103 88-89 0.5131620179524106 1103 90-91 0.5028786823654521 1103 92-93 0.5758519554952777 1103 94-95 0.7501266103460438 1103 96-97 0.7473944304749658 1103 98-99 0.998182058308938 1103 100-101 1.0742200154966923 1104 1 0.16556151803299812 1104 2 6.454169723433267E-4 1104 3 0.02846724608949458 1104 4 -0.022231967095329708 1104 5 -0.01846384831725345 1104 6 -0.015371263424704296 1104 7 0.01789002930448902 1104 8 0.07634874944540115 1104 9 0.16926028611818822 1104 10-11 0.1598120430199259 1104 12-13 0.17726931908587318 1104 14-15 0.31256594948718686 1104 16-17 0.3119433327077843 1104 18-19 0.241266803178533 1104 20-21 0.24538406201985197 1104 22-23 0.2659082198419611 1104 24-25 0.2287818244725912 1104 26-27 0.20006989297264255 1104 28-29 0.2359343424621585 1104 30-31 0.1799002231211233 1104 32-33 -0.03640439435363163 1104 34-35 0.12131679794746475 1104 36-37 0.22649561647354233 1104 38-39 0.11761363502601085 1104 40-41 0.19080349502860372 1104 42-43 0.3819844001506354 1104 44-45 0.29024877122486004 1104 46-47 0.297934946674701 1104 48-49 0.3956737217259061 1104 50-51 0.22880420325144257 1104 52-53 0.2431629104693087 1104 54-55 0.24599312013109653 1104 56-57 0.3912432855605843 1104 58-59 0.43305025376376705 1104 60-61 0.4292280021180659 1104 62-63 0.2652654057721051 1104 64-65 0.2168586938671524 1104 66-67 0.41501102781073484 1104 68-69 0.3684764557174631 1104 70-71 0.3863908609847968 1104 72-73 0.512699470769757 1104 74-75 0.4949645408901091 1104 76-77 0.4777008802450631 1104 78-79 0.4045122637380061 1104 80-81 0.4726907770742166 1104 82-83 0.3402310851498136 1104 84-85 0.5370112125671618 1104 86-87 0.48188507389243895 1104 88-89 0.2640948428366592 1104 90-91 0.43219144238509344 1104 92-93 0.7579929346129397 1104 94-95 0.7387245006181331 1104 96-97 0.7908634404539043 1104 98-99 1.0250341276755925 1104 100-101 1.0612885636334077 1105 1 0.08519531349501364 1105 2 0.09918424821681526 1105 3 0.06577014556486205 1105 4 0.11896249659082514 1105 5 0.08223406669796418 1105 6 0.15012309518105127 1105 7 0.20396993228353466 1105 8 0.12217516893881708 1105 9 0.08805373754553614 1105 10-11 0.12705486419991985 1105 12-13 0.18001331050850666 1105 14-15 0.3127657261646348 1105 16-17 0.1267797316338246 1105 18-19 0.16814693674473347 1105 20-21 0.2637588157176438 1105 22-23 0.2695595392440566 1105 24-25 0.364878671077868 1105 26-27 0.3865258461680625 1105 28-29 0.4406724256230987 1105 30-31 0.25664213686631143 1105 32-33 0.16986865741780122 1105 34-35 0.2779044285447938 1105 36-37 0.3173405884898486 1105 38-39 0.34850504924404646 1105 40-41 0.4155621925786761 1105 42-43 0.43789975552022753 1105 44-45 0.354369072253057 1105 46-47 0.4978979399147647 1105 48-49 0.5659613317686691 1105 50-51 0.47702768647639004 1105 52-53 0.5190504423961926 1105 54-55 0.6177908709234643 1105 56-57 0.6130445404572527 1105 58-59 0.623205921460162 1105 60-61 0.6925017200966366 1105 62-63 0.726175626774257 1105 64-65 0.7875201320113803 1105 66-67 0.8287184000615611 1105 68-69 0.9906214119653001 1105 70-71 0.8751138414562547 1105 72-73 1.024319956618097 1105 74-75 0.8005432672165753 1105 76-77 0.8284302039999432 1105 78-79 0.8262880497791052 1105 80-81 0.8756289044210597 1105 82-83 0.9526448873892797 1105 84-85 1.0388142237892701 1105 86-87 1.0311940175203738 1105 88-89 0.9005967980841163 1105 90-91 1.095440076104918 1105 92-93 1.2135490338269541 1105 94-95 1.255513006130471 1105 96-97 1.3829592067694598 1105 98-99 1.3264135909750152 1105 100-101 1.6769188772122838 1106 1 -0.05973525718415118 1106 2 -0.03783808601541594 1106 3 -0.001127745984458528 1106 4 0.0589497659173972 1106 5 -0.04456264704231927 1106 6 -0.06190540714882076 1106 7 -0.027548824918902426 1106 8 -0.09943938576421374 1106 9 -0.10371095927466456 1106 10-11 -0.38035817379299175 1106 12-13 -0.4493689857324412 1106 14-15 -0.34827997704633873 1106 16-17 -0.24703301169092384 1106 18-19 -0.06371051145783468 1106 20-21 -0.14763649083273123 1106 22-23 -0.13493321671332126 1106 24-25 -0.11595225862309633 1106 26-27 -0.055331226229881736 1106 28-29 0.0033138046066625293 1106 30-31 -0.13234564388132952 1106 32-33 0.13638878362279172 1106 34-35 -0.02522646747380719 1106 36-37 -0.15992416540130705 1106 38-39 -0.191924642495799 1106 40-41 -0.20276584564415856 1106 42-43 -0.1917039386625703 1106 44-45 -0.09028774459773814 1106 46-47 -0.05972131211265719 1106 48-49 -0.17694698987620683 1106 50-51 -0.19834224182428528 1106 52-53 -0.3718916391085969 1106 54-55 -0.3508638722327433 1106 56-57 -0.3908695464040335 1106 58-59 -0.36132691721158494 1106 60-61 -0.43046692169176026 1106 62-63 -0.3570387212128949 1106 64-65 -0.23307816295603345 1106 66-67 -0.1651346267179008 1106 68-69 -0.2721778283024676 1106 70-71 -0.4112565184361969 1106 72-73 -0.0743936478127587 1106 74-75 -0.3192378044148185 1106 76-77 -0.20488977776536288 1106 78-79 -0.25669533008630907 1106 80-81 -0.3113866489885986 1106 82-83 -0.24924727722435236 1106 84-85 -0.36279837486236133 1106 86-87 -0.09987368076500047 1106 88-89 -0.0925171073639035 1106 90-91 0.15222403816406072 1106 92-93 0.14723095512692908 1106 94-95 0.1953536883115703 1106 96-97 0.23125715027785532 1106 98-99 0.22648212279971247 1106 100-101 0.2116792877589937 1107 1 0.06535227803718158 1107 2 0.12481439501105385 1107 3 0.25009735236683994 1107 4 0.13440413983627764 1107 5 0.09671426584404941 1107 6 0.10614090145043775 1107 7 0.20211517404528223 1107 8 0.23810432263434933 1107 9 0.18665254775538642 1107 10-11 0.28931054634930575 1107 12-13 0.38979160774649557 1107 14-15 0.5255465378162896 1107 16-17 0.5413105732306818 1107 18-19 0.43447088757407926 1107 20-21 0.2269203065979397 1107 22-23 0.3946088842740991 1107 24-25 0.31141620564397954 1107 26-27 0.3848341571132039 1107 28-29 0.4057464745276249 1107 30-31 0.4904598570017171 1107 32-33 0.4899718656994523 1107 34-35 0.6557434740957007 1107 36-37 0.5846518088548578 1107 38-39 0.6435296682635183 1107 40-41 0.5364099850818675 1107 42-43 0.5077539942982767 1107 44-45 0.556292993469242 1107 46-47 0.8188425655062019 1107 48-49 0.6894495906644877 1107 50-51 0.6863390352307093 1107 52-53 0.7998802948296486 1107 54-55 0.8610530760861437 1107 56-57 0.9192644861057744 1107 58-59 0.9544812134782035 1107 60-61 1.0067777261265078 1107 62-63 1.02959892362977 1107 64-65 1.0288781416720596 1107 66-67 1.0718073288446064 1107 68-69 1.067728048143195 1107 70-71 0.9359332358177994 1107 72-73 0.8870474325949083 1107 74-75 0.8995990646703831 1107 76-77 0.8465979065125602 1107 78-79 0.9427815507465773 1107 80-81 1.1210633977100102 1107 82-83 1.0813003241724246 1107 84-85 1.1925128320934029 1107 86-87 1.1237108145071097 1107 88-89 1.1350906527370874 1107 90-91 1.2863532610723531 1107 92-93 1.2619504516188087 1107 94-95 1.2010494975949229 1107 96-97 1.1557550119315465 1107 98-99 1.1198762630123902 1107 100-101 1.2258372956428865 1108 1 -0.009018332452004785 1108 2 0.0031592986827320146 1108 3 -0.020903992109314373 1108 4 0.08428570157524007 1108 5 0.02444718904894927 1108 6 0.16892724120118174 1108 7 0.18504747369808427 1108 8 0.11501805969238177 1108 9 0.1290276444758831 1108 10-11 0.13747220548086858 1108 12-13 0.15072305545911746 1108 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-1.8220550752780866 2316 66-67 -2.028275040946582 2316 68-69 -2.0311495097769097 2316 70-71 -2.173960769423541 2316 72-73 -1.8522829132967473 2316 74-75 -1.8392958687678558 2316 76-77 -2.001265908766012 2316 78-79 -1.961215638025724 2316 80-81 -2.2269172105560635 2316 82-83 -2.416598426552408 2316 84-85 -2.491098038260237 2316 86-87 -2.552278801162 2316 88-89 -2.8371525717328865 2316 90-91 -2.8578594414968883 2316 92-93 -2.802129967524589 2316 94-95 -2.849442979502385 2316 96-97 -2.620852786971419 2316 98-99 -3.028544060606638 2316 100-101 -3.5772394824278955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5912.0 3 1302.0 4 1145.0 5 1251.0 6 1414.0 7 1759.0 8 2045.0 9 2666.0 10 3201.0 11 4405.0 12 5964.0 13 7862.0 14 9049.0 15 11874.0 16 15084.0 17 21024.0 18 17396.0 19 10919.0 20 10291.0 21 10878.0 22 11505.0 23 12619.0 24 13989.0 25 15734.0 26 18171.0 27 21304.0 28 25256.0 29 30170.0 30 37276.0 31 46875.0 32 59912.0 33 77614.0 34 107153.0 35 151735.0 36 226733.0 37 333899.0 38 290405.0 39 41244.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13109132475033 19.016363855131754 15.001323547106244 21.85122127301167 2 15.037240840136516 20.289266759881528 41.227068392645045 23.446424007336912 3 18.900110532589075 25.564359674728692 30.300963762389816 25.234566030292427 4 12.159477789264587 16.76953674485514 38.02446148822121 33.04652397765906 5 13.027778947178504 37.80438665600173 34.377033504333134 14.790800892486633 6 30.49264986073863 36.64196785869269 18.2817058525837 14.583676427984985 7 27.747889382488122 33.57534519894613 20.995535971845612 17.681229446720128 8 26.532637223220206 34.487079552837194 20.034701333543282 18.945581890399318 9 26.666706730769228 16.522896634615382 20.79032451923077 36.020072115384615 10-11 24.37647138027304 27.1797316268503 27.483317218842203 20.96047977403446 12-13 25.6211142792574 24.667895592216297 28.348902261351235 21.362087867175067 14-15 22.667609835722708 25.4897547560121 27.284430378377465 24.558205029887723 16-17 21.186444497934183 29.102385071434345 27.772503929843097 21.938666500788372 18-19 21.18786520932508 26.964415247397294 31.080451674125857 20.767267869151766 20-21 22.071445548076817 25.977336625300733 31.221498386016528 20.72971944060592 22-23 21.6619330986381 26.00035937456806 31.08713210681237 21.250575419981466 24-25 21.29494954654232 26.172522732974496 31.347153139050842 21.185374581432345 26-27 21.18137293636956 26.442952657169716 31.15842726775886 21.217247138701865 28-29 20.998382474594514 26.487992660887887 31.354571605172353 21.159053259345242 30-31 21.22795028480037 26.270332258081964 31.319956701448554 21.18176075566911 32-33 20.96157012922543 26.407975412190776 31.16083523547491 21.46961922310889 34-35 21.337836786003756 26.36707627089356 31.087887950218796 21.207198992883892 36-37 21.337862382798825 26.33638257948497 30.972929364555927 21.352825673160275 38-39 21.237603755868545 26.442967136150237 31.008120187793427 21.311308920187795 40-41 21.3732657584032 26.380602934389664 30.987550236520267 21.25858107068687 42-43 21.082533764577633 26.429217882936644 30.86653902412348 21.621709328362247 44-45 21.29876248810085 26.676939201338474 30.509818604025043 21.51447970653564 46-47 21.4044739382863 26.900964015516323 29.9265201476869 21.76804189851048 48-49 21.496445727284886 26.971393025481678 29.72093211947645 21.811229127756985 50-51 21.750359111207274 27.19929319702133 29.488589579464247 21.561758112307146 52-53 21.75782217402699 27.316116094936056 29.24650337460117 21.679558356435784 54-55 21.482617848090044 27.367922841486376 29.2997104602435 21.849748850180077 56-57 21.49839335529607 27.664964629780687 29.144219459103795 21.692422555819448 58-59 21.61165051460746 27.303835481850154 29.272819321778766 21.811694681763623 60-61 21.555522172815767 27.226174738613146 29.590193486359812 21.628109602211275 62-63 21.50237100398242 27.27598053679458 29.695330044595668 21.52631841462733 64-65 21.352991792984678 27.152613950604078 29.707977353492936 21.786416902918305 66-67 21.34526634001017 27.395341584769707 29.368589962177825 21.890802113042295 68-69 21.291479346400987 27.40509006394426 29.47958314255808 21.82384744709667 70-71 21.382926109611777 27.350753242195502 29.308690183663817 21.957630464528897 72-73 21.402601070534168 27.33834197903099 29.12294617733971 22.13611077309513 74-75 21.275810688714703 27.59930735816272 29.0585064812731 22.06637547184948 76-77 21.434494918203907 27.420275624264985 29.01314061789208 22.13208883963903 78-79 21.46863356211408 27.401318962100795 29.018758658899955 22.111288816885168 80-81 21.354523471283763 27.644710938683087 28.90637206473743 22.094393525295718 82-83 21.380421203302372 27.62536978626159 28.944942633929777 22.04926637650626 84-85 21.391390350842933 27.39271587364631 28.856439150631907 22.35945462487885 86-87 21.41884600316776 27.367497302117204 28.970213931890775 22.243442762824255 88-89 21.574060582390096 27.071660870806134 29.02213442891371 22.332144117890056 90-91 21.45261165442336 26.993645500022534 29.206393558369765 22.34734928718434 92-93 21.38132495624079 26.908926158244604 29.25993603013061 22.449812855383993 94-95 21.140593093266766 26.962319016484358 29.335695523785287 22.561392366463583 96-97 21.17541273345769 27.01642521888325 29.15423026070112 22.653931786957944 98-99 21.02570789552434 27.209766139993917 28.96014080393809 22.80438516054365 100-101 21.11831266200485 27.05410436327061 29.28002249591721 22.547560478807334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2221.0 1 1292.0 2 423.0 3 506.5 4 432.0 5 403.5 6 740.0 7 1495.5 8 2549.0 9 3247.5 10 3047.0 11 2691.0 12 2762.0 13 2983.5 14 3185.5 15 3392.0 16 3538.0 17 3624.5 18 3786.5 19 4069.0 20 4445.5 21 4963.0 22 5714.0 23 6609.0 24 7514.0 25 9018.5 26 11340.5 27 14205.5 28 16887.5 29 20152.0 30 23724.0 31 26242.5 32 29099.5 33 32372.5 34 36247.5 35 39718.0 36 43667.0 37 47612.0 38 49624.0 39 51106.0 40 52237.0 41 53756.5 42 55281.5 43 57557.5 44 60150.5 45 60997.5 46 60466.0 47 61106.5 48 64214.5 49 63683.0 50 59700.5 51 56752.5 52 54074.0 53 51612.5 54 49550.5 55 45649.5 56 39685.0 57 35044.0 58 31902.0 59 28085.0 60 23548.0 61 19264.0 62 15061.0 63 11271.0 64 8375.0 65 6254.0 66 4531.5 67 3304.5 68 2410.0 69 1676.0 70 1157.5 71 830.5 72 640.0 73 460.0 74 335.5 75 261.0 76 172.5 77 105.5 78 78.5 79 55.0 80 31.5 81 26.5 82 26.5 83 15.5 84 10.5 85 9.5 86 10.5 87 9.0 88 7.0 89 6.5 90 4.5 91 3.0 92 3.0 93 4.0 94 4.0 95 4.0 96 5.5 97 9.5 98 9.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2907536500170661 2 0.25326220556469015 3 0.2512826572976047 4 0.25614154849863263 5 0.2565614526764992 6 0.1960952510637073 7 0.18469785195018498 8 0.18799709906199405 9 0.18277828999422333 10-11 0.2057830403102012 12-13 0.19573533319696446 14-15 0.18913683897334627 16-17 0.19054651728475563 18-19 0.18619750972828 20-21 0.17974898128247133 22-23 0.18265831737197571 24-25 0.17333044599222455 26-27 0.1738703227923388 28-29 0.18508776297248966 30-31 0.19495551115235504 32-33 0.18793711275087024 34-35 0.19714501150837377 36-37 0.17842928243774772 38-39 0.17902914554898572 40-41 0.17510004217037672 42-43 0.18145859114949967 44-45 0.18373807097220413 46-47 0.18724727017294654 48-49 0.19222613399622207 50-51 0.19297596288526958 52-53 0.2065328691992487 54-55 0.19192620244060304 56-57 0.17183078821412953 58-59 0.1748301037703196 60-61 0.170781027769463 62-63 0.17899915239342382 64-65 0.1716208361251962 66-67 0.16865151372456805 68-69 0.16706187647978732 70-71 0.1783992892821858 72-73 0.17542996688155763 74-75 0.1789691592378619 76-77 0.1781593440376906 78-79 0.17608981630391946 80-81 0.1782193303488144 82-83 0.1745601653702625 84-85 0.16784169852439673 86-87 0.1667019586130445 88-89 0.16703188332422542 90-91 0.17228068554755804 92-93 0.16952131523586317 94-95 0.16631204759073978 96-97 0.16475240350152096 98-99 0.17330045283666268 100-101 0.16466242403483525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1667047.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.49386286864722 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0641797107133 46.24769285820543 2 12.03414672424495 14.078474564583285 3 3.7054602726503907 6.502400551608963 4 1.6686463471623285 3.9042228242872934 5 0.886351311653252 2.592305603864546 6 0.5496090372910499 1.9289253395203136 7 0.3705091097277898 1.517075633920133 8 0.262386348626023 1.2278392876108521 9 0.1961645514815546 1.0326980136646569 >10 1.1497045920832771 12.415963193307682 >50 0.07826076445520996 3.1549814679813104 >100 0.032842111519006886 3.217933479137811 >500 0.001125311900659049 0.44197716568351875 >1k 3.069032456342861E-4 0.33707790066277565 >5k 2.0460216375619073E-4 0.7865350481646587 >10k+ 1.0230108187809536E-4 0.6138970677968073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10259 0.6153995658190801 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7899 0.47383187156690837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.3146282018443391 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2103 0.12615121229335466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2095 0.12567132180436424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9993155561900776E-5 2.9993155561900776E-5 16-17 0.0 0.0 0.0 8.997946668570233E-5 5.998631112380155E-5 18-19 0.0 0.0 0.0 1.7995893337140463E-4 5.998631112380155E-5 20-21 0.0 0.0 0.0 2.0995208893330542E-4 5.998631112380155E-5 22-23 0.0 0.0 0.0 4.1990417786661084E-4 1.1997262224760309E-4 24-25 0.0 0.0 0.0 5.998631112380155E-4 1.7995893337140463E-4 26-27 0.0 0.0 0.0 0.0010197672891046263 1.7995893337140463E-4 28-29 0.0 0.0 0.0 0.002129514044894955 1.7995893337140463E-4 30-31 0.0 0.0 0.0 0.0046189459565327195 1.7995893337140463E-4 32-33 0.0 0.0 0.0 0.007498288890475193 1.7995893337140463E-4 34-35 0.0 0.0 0.0 0.010407624979979569 1.7995893337140463E-4 36-37 0.0 0.0 0.0 0.014216755736340967 1.7995893337140463E-4 38-39 0.0 0.0 0.0 0.017246064448092943 1.7995893337140463E-4 40-41 0.0 0.0 0.0 0.02261483929367318 1.7995893337140463E-4 42-43 0.0 0.0 0.0 0.030443052895329285 1.7995893337140463E-4 44-45 0.0 0.0 0.0 0.043460082409194224 1.7995893337140463E-4 46-47 0.0 0.0 0.0 0.06862433992562897 1.7995893337140463E-4 48-49 0.0 0.0 0.0 0.12378175300396449 1.7995893337140463E-4 50-51 0.0 0.0 0.0 0.16466242403483525 1.7995893337140463E-4 52-53 0.0 0.0 0.0 0.20332360155412535 1.7995893337140463E-4 54-55 0.0 0.0 0.0 0.2560815621875088 1.7995893337140463E-4 56-57 0.0 0.0 0.0 0.29306312299533244 1.7995893337140463E-4 58-59 0.0 0.0 0.0 0.34501126842854457 1.7995893337140463E-4 60-61 0.0 0.0 0.0 0.4301018507576571 1.7995893337140463E-4 62-63 0.0 0.0 0.0 0.487568736814259 1.7995893337140463E-4 64-65 0.0 0.0 0.0 0.5576627413624211 1.7995893337140463E-4 66-67 0.0 0.0 0.0 0.6986905588144785 1.7995893337140463E-4 68-69 0.0 0.0 0.0 0.8414579792891261 1.7995893337140463E-4 70-71 0.0 0.0 0.0 0.9706684934497949 1.7995893337140463E-4 72-73 0.0 0.0 0.0 1.1364406642404203 1.7995893337140463E-4 74-75 0.0 0.0 0.0 1.283437119649296 1.7995893337140463E-4 76-77 0.0 0.0 0.0 1.402839871941223 1.7995893337140463E-4 78-79 0.0 0.0 0.0 1.56816214539842 1.7995893337140463E-4 80-81 0.0 0.0 0.0 1.8034284576259698 1.7995893337140463E-4 82-83 0.0 0.0 0.0 2.0183294172269886 1.7995893337140463E-4 84-85 0.0 0.0 0.0 2.2655629985237367 1.7995893337140463E-4 86-87 0.0 0.0 0.0 2.7195094079531055 1.7995893337140463E-4 88-89 0.0 0.0 0.0 3.3559041826655154 1.7995893337140463E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 44.32179 1 GTATCAA 9365 0.0 39.90024 1 TATCAAC 11720 0.0 31.750551 2 ATCAACG 11820 0.0 31.321102 3 TCAACGC 11950 0.0 30.865707 4 CAACGCA 12195 0.0 30.16947 5 AACGCAG 12480 0.0 29.578754 6 GTACATG 8825 0.0 26.019154 1 ACGCAGA 14330 0.0 25.561222 7 CGCAGAG 14325 0.0 25.402792 8 TACATGG 9080 0.0 24.861357 2 GTACCGT 100 0.001652187 23.754366 6 CCGATCG 160 1.857903E-6 23.75365 9 GCAGAGT 15170 0.0 23.612726 9 ACATGGG 9310 0.0 23.43042 3 GAGTACT 8230 0.0 22.36692 12-13 AGTACTT 8540 0.0 22.27814 12-13 TACCTGG 1425 0.0 21.677818 2 GTGTAGC 1080 0.0 21.128965 1 GTACTTT 8785 0.0 20.7357 14-15 >>END_MODULE