FastQCFastQC Report
Thu 26 May 2016
SRR937398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667047
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104690.6279966911550784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87150.5227807014439305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55590.3334639035372128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.13784854296249596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63300.041.9659841
GTATCAA105000.036.840411
ATCAACG135350.028.3946323
TATCAAC136400.028.210882
TCAACGC137150.027.9873354
CAACGCA140650.027.2908865
AACGCAG143650.026.782266
TATCACG1201.7112592E-423.7500157
GTACATG97200.023.4673631
ACGCAGA164200.023.3739477
CGCAGAG166000.022.948818
TACATGG99950.022.4814512
AGTACTT94500.022.41800112-13
GAGTACT91250.022.20138412-13
GACCGGT1302.9422657E-421.923097
ACATGGG100500.021.5548383
GCAGAGT173950.021.4903859
GTACTTT95000.021.02375214-15
GTACCTG15950.020.8557931
CATGGGG36000.020.1899344
Produced by FastQC (version 0.11.5)