##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1667047 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24078925189272 33.0 31.0 34.0 31.0 34.0 2 32.459031449023335 34.0 31.0 34.0 31.0 34.0 3 32.53266164661224 34.0 31.0 34.0 31.0 34.0 4 36.13639867382263 37.0 35.0 37.0 35.0 37.0 5 36.01074174873294 37.0 35.0 37.0 35.0 37.0 6 36.09866608439954 37.0 35.0 37.0 35.0 37.0 7 36.06400659369532 37.0 35.0 37.0 35.0 37.0 8 36.04637301767737 37.0 35.0 37.0 35.0 37.0 9 37.75218875052713 39.0 37.0 39.0 35.0 39.0 10-11 37.67771424560915 39.0 37.0 39.0 35.0 39.0 12-13 37.59205679263992 39.0 37.0 39.0 35.0 39.0 14-15 38.813643826478796 40.0 38.0 41.0 35.0 41.0 16-17 38.80098761462634 40.0 38.0 41.0 35.0 41.0 18-19 38.82567348131157 40.0 38.0 41.0 35.0 41.0 20-21 38.74969961854705 40.0 38.0 41.0 34.5 41.0 22-23 38.623747260875064 40.0 38.0 41.0 34.0 41.0 24-25 38.48916857173193 40.0 38.0 41.0 34.0 41.0 26-27 38.274419677429606 40.0 38.0 41.0 34.0 41.0 28-29 38.25245209043297 40.0 38.0 41.0 34.0 41.0 30-31 38.34763297015621 40.0 38.0 41.0 34.0 41.0 32-33 38.29152327438878 40.0 38.0 41.0 34.0 41.0 34-35 38.17493118070456 40.0 38.0 41.0 34.0 41.0 36-37 38.025985170184164 40.0 37.0 41.0 33.5 41.0 38-39 37.88245712328447 40.0 37.0 41.0 33.0 41.0 40-41 37.71071421501613 40.0 37.0 41.0 33.0 41.0 42-43 37.48991960034721 40.0 37.0 41.0 33.0 41.0 44-45 37.17216671155643 40.0 37.0 41.0 32.5 41.0 46-47 36.77204331971444 40.0 36.0 41.0 31.0 41.0 48-49 36.40447719830335 40.0 36.0 41.0 30.0 41.0 50-51 36.17513663382016 39.0 36.0 41.0 30.0 41.0 52-53 35.938715285171924 39.0 35.5 41.0 29.0 41.0 54-55 35.732808073197695 39.0 35.0 40.5 28.5 41.0 56-57 35.49509731879185 39.0 35.0 40.0 28.0 41.0 58-59 35.25126316174649 38.5 35.0 40.0 28.0 41.0 60-61 34.932147683898535 38.0 34.5 40.0 27.0 41.0 62-63 34.56848487175226 38.0 34.0 40.0 26.0 41.0 64-65 34.22479660141556 37.0 34.0 40.0 26.0 41.0 66-67 33.84827452375368 36.5 34.0 39.5 25.5 41.0 68-69 33.41714810680203 36.0 33.0 39.0 24.5 40.5 70-71 33.03842993028991 35.5 33.0 39.0 23.5 40.0 72-73 32.615817970339165 35.0 32.5 38.0 22.5 40.0 74-75 32.17996073296074 35.0 32.0 37.0 21.5 39.0 76-77 30.86236680789444 34.0 30.5 36.0 20.0 38.5 78-79 31.463660592652758 35.0 31.5 36.0 20.0 39.0 80-81 31.29402980239909 35.0 32.0 36.0 20.0 37.5 82-83 30.9595101397861 34.5 31.0 35.5 18.5 37.0 84-85 30.63242728009468 34.0 31.0 35.0 17.5 37.0 86-87 30.312968980478654 34.0 31.0 35.0 13.5 36.0 88-89 29.96753120937802 34.0 31.0 35.0 7.5 36.0 90-91 29.545497217534958 34.0 30.0 35.0 4.0 35.5 92-93 29.160739319287337 34.0 29.5 35.0 2.0 35.0 94-95 28.797448122338484 34.0 29.0 35.0 2.0 35.0 96-97 28.413873154146223 34.0 29.0 35.0 2.0 35.0 98-99 27.82288321804964 34.0 29.0 35.0 2.0 35.0 100-101 26.28049839026734 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01564608365489306 1101 2 0.056047989238578566 1101 3 0.02496333490793745 1101 4 0.04957673276115315 1101 5 -0.044374994293242764 1101 6 0.057889655915197125 1101 7 0.015484661174902214 1101 8 -0.0414805308567594 1101 9 -0.021190280020221053 1101 10-11 0.048308901513642866 1101 12-13 0.07384255273082374 1101 14-15 0.07756084744717384 1101 16-17 0.004274249639401262 1101 18-19 0.10446201113318665 1101 20-21 0.0011439335119902694 1101 22-23 0.00915163842329747 1101 24-25 0.08933831560891292 1101 26-27 0.021233523069646765 1101 28-29 0.08685372965015148 1101 30-31 0.037942491848014015 1101 32-33 0.11669204193368188 1101 34-35 0.3102078378115536 1101 36-37 0.2038916897565386 1101 38-39 0.12743450844737225 1101 40-41 0.1398302009118808 1101 42-43 0.05276658196311246 1101 44-45 0.13841075771747313 1101 46-47 0.027056462849408547 1101 48-49 0.030258550131463835 1101 50-51 0.07273210502038552 1101 52-53 0.12793792737677023 1101 54-55 -0.0019077497725916714 1101 56-57 -0.033750064888174336 1101 58-59 0.001418699234363885 1101 60-61 0.08107203483376679 1101 62-63 0.02076973216035327 1101 64-65 0.15776517280047386 1101 66-67 0.022526624986547006 1101 68-69 -0.14265186732334456 1101 70-71 -0.2892893879341827 1101 72-73 -0.19537950319545416 1101 74-75 -0.1863629297093894 1101 76-77 -0.07183035204270993 1101 78-79 -0.045854984894074136 1101 80-81 -0.1637557799705256 1101 82-83 -0.18886221987529694 1101 84-85 -0.28697699718518166 1101 86-87 -0.33775683583602145 1101 88-89 -0.2309431073813748 1101 90-91 -0.17128515122638532 1101 92-93 -0.00210360001740284 1101 94-95 -0.18665885927530113 1101 96-97 -0.2019561382399928 1101 98-99 -0.2549794115350501 1101 100-101 0.04040640504543802 1102 1 0.14212921630247166 1102 2 0.060475645091251806 1102 3 0.01318073261703745 1102 4 0.03314916108526944 1102 5 -0.06303110335886686 1102 6 0.029016375043468656 1102 7 0.011454912412538931 1102 8 0.08115344658644119 1102 9 0.12238374594488022 1102 10-11 0.09166960804889612 1102 12-13 0.05492285437574651 1102 14-15 0.01168209980873769 1102 16-17 0.07730995648602601 1102 18-19 0.058403245431101425 1102 20-21 0.10687791855686868 1102 22-23 0.10492162622195877 1102 24-25 0.09887155105094791 1102 26-27 0.14113014453137396 1102 28-29 0.047112732163853366 1102 30-31 0.04072547937654036 1102 32-33 0.04306054813506677 1102 34-35 0.12129409195758001 1102 36-37 0.07778574210848177 1102 38-39 -0.002134068359431751 1102 40-41 0.08624394783954159 1102 42-43 0.16987248537541433 1102 44-45 0.0010776242324155305 1102 46-47 0.11067460861524836 1102 48-49 0.08000681430740997 1102 50-51 0.09179084538353521 1102 52-53 0.114506362027889 1102 54-55 0.10926583778607579 1102 56-57 0.15644619585885522 1102 58-59 0.035134077774351624 1102 60-61 -0.024213902103220164 1102 62-63 0.07447462725503584 1102 64-65 0.15299028771681122 1102 66-67 0.2694306587294122 1102 68-69 0.1355802097860277 1102 70-71 0.1639974362948209 1102 72-73 0.20416892701490497 1102 74-75 0.18367058639349665 1102 76-77 0.12255746156412073 1102 78-79 0.2536319447276938 1102 80-81 0.26129177346992094 1102 82-83 0.32450750130628947 1102 84-85 0.25987210691186746 1102 86-87 0.14183609211166015 1102 88-89 0.28535036123034985 1102 90-91 0.22503782650155202 1102 92-93 0.15050461331749077 1102 94-95 0.09446722423236409 1102 96-97 0.27361079883743145 1102 98-99 0.4625528301055688 1102 100-101 0.44245340403574573 1103 1 0.008969508873512666 1103 2 0.06945858234423952 1103 3 0.02507607055017047 1103 4 0.039614043095269835 1103 5 -0.13721301081719162 1103 6 0.04298596098301033 1103 7 0.03959890110646569 1103 8 -0.037288227947271935 1103 9 -0.07294562130156379 1103 10-11 0.10494672879661238 1103 12-13 0.15198803850741172 1103 14-15 0.16317055149029613 1103 16-17 0.027198633713389597 1103 18-19 -0.033438098203177447 1103 20-21 0.0054715622210252945 1103 22-23 0.06665606716985195 1103 24-25 0.04441208645693706 1103 26-27 0.026064912286869912 1103 28-29 0.0635283164976812 1103 30-31 0.0387873637317 1103 32-33 0.2056802653619556 1103 34-35 0.26060178864073436 1103 36-37 0.20413770732694303 1103 38-39 0.19453211642940715 1103 40-41 0.3040800076046821 1103 42-43 0.3245940525410447 1103 44-45 0.2170178357085888 1103 46-47 0.16017800090779843 1103 48-49 0.16032794279185225 1103 50-51 0.09860859236709985 1103 52-53 0.3052863678598854 1103 54-55 0.3136546956614197 1103 56-57 0.31679786475617533 1103 58-59 0.19004629769410286 1103 60-61 0.19713327094135735 1103 62-63 0.15582197566417477 1103 64-65 0.28221800691857624 1103 66-67 0.22849034046071637 1103 68-69 0.1642796320047637 1103 70-71 0.12654484936402355 1103 72-73 0.20064241312974218 1103 74-75 0.20770913332274432 1103 76-77 0.06447177259385484 1103 78-79 0.24415562602553464 1103 80-81 0.3312596480312031 1103 82-83 0.16901815211789994 1103 84-85 0.11169883233615252 1103 86-87 0.42474982826038854 1103 88-89 0.42546113767492955 1103 90-91 0.26267004969477625 1103 92-93 0.3265724317396277 1103 94-95 0.18218064346585194 1103 96-97 0.18321239078792573 1103 98-99 0.14143277665085563 1103 100-101 0.49230808751732624 1104 1 -0.009681995218407735 1104 2 0.06315992466545595 1104 3 0.06592995758467168 1104 4 -0.0032887368596661304 1104 5 -0.1139693551770975 1104 6 -0.006269971948256625 1104 7 0.004312436044898504 1104 8 0.008274400176382812 1104 9 0.05490012965015012 1104 10-11 0.08330764485605613 1104 12-13 0.06444644513438647 1104 14-15 0.008141091462704253 1104 16-17 0.032461120844033076 1104 18-19 0.004449486031134597 1104 20-21 -0.03747941676203226 1104 22-23 -0.10408855570749864 1104 24-25 -0.13111965520842972 1104 26-27 -0.16340058220259124 1104 28-29 -0.11935258283539696 1104 30-31 -0.04370147341681019 1104 32-33 0.005608487890356173 1104 34-35 -0.07479451375562007 1104 36-37 0.06374533684228822 1104 38-39 -0.10839823250116609 1104 40-41 -0.09256933491280961 1104 42-43 0.0425422416254051 1104 44-45 0.06438367197691974 1104 46-47 0.16079199573443503 1104 48-49 0.071286968841477 1104 50-51 0.22481069132621911 1104 52-53 0.3041209066755144 1104 54-55 0.21401775158401648 1104 56-57 0.18194110922816265 1104 58-59 0.2325833512397466 1104 60-61 0.09273634716833357 1104 62-63 0.05092536692173866 1104 64-65 0.21861124759109174 1104 66-67 0.0622135720883179 1104 68-69 0.09741390190455235 1104 70-71 0.01806251517627544 1104 72-73 0.0037774993734416284 1104 74-75 -0.1493796627480357 1104 76-77 0.04101480368743893 1104 78-79 0.10198455942446216 1104 80-81 0.18533415938641085 1104 82-83 0.15933188436839174 1104 84-85 0.14488831400554147 1104 86-87 0.34518202590593106 1104 88-89 0.24995403830546792 1104 90-91 0.1432294927745481 1104 92-93 -0.018653324257787318 1104 94-95 -0.07895604593830541 1104 96-97 0.04170416937983035 1104 98-99 0.0861228720979561 1104 100-101 0.1155107739835195 1105 1 0.005864048925609211 1105 2 0.014958192526883352 1105 3 0.022299799553842092 1105 4 -0.023208287753149648 1105 5 -0.05263428289640615 1105 6 -0.014073013938109113 1105 7 -0.002671842064742691 1105 8 0.0025334380946375745 1105 9 0.06876035776733858 1105 10-11 0.00207983662627953 1105 12-13 0.023688584296046145 1105 14-15 -0.0029821961734057822 1105 16-17 0.018171319454097556 1105 18-19 -0.025985903200066218 1105 20-21 -0.02017768817029264 1105 22-23 -0.04947281706581208 1105 24-25 -0.10893055579314392 1105 26-27 -0.06446615040378134 1105 28-29 -0.21755670802080118 1105 30-31 -0.22065841951300058 1105 32-33 -0.13631735073366968 1105 34-35 -0.19078431852145883 1105 36-37 -0.300858316912894 1105 38-39 -0.0903252804685053 1105 40-41 -0.1332116030425965 1105 42-43 -0.19268592898003334 1105 44-45 -0.014870620444000338 1105 46-47 -0.07861887886863173 1105 48-49 0.03071926976028294 1105 50-51 -0.0037194052973248404 1105 52-53 -0.01267768381989498 1105 54-55 0.11879179454690103 1105 56-57 0.04331810256138624 1105 58-59 0.02637632192108441 1105 60-61 0.08165312170016392 1105 62-63 0.008621533730782005 1105 64-65 0.19917383153468649 1105 66-67 0.0700889396453519 1105 68-69 -0.02732152753838335 1105 70-71 0.09678328635271072 1105 72-73 0.074546197295291 1105 74-75 0.16539915348861456 1105 76-77 0.08747997438646138 1105 78-79 0.09926460530259007 1105 80-81 0.11747237237014474 1105 82-83 0.023185894837119747 1105 84-85 0.12711086052359377 1105 86-87 0.11241324508209516 1105 88-89 0.05889050846737831 1105 90-91 -0.0654494257221252 1105 92-93 0.19473081573887185 1105 94-95 0.21901588960497875 1105 96-97 0.20986527954844192 1105 98-99 0.2464153451537463 1105 100-101 0.06175343406018996 1106 1 -0.12354112237944292 1106 2 -0.0704619652130205 1106 3 -0.1048874242093305 1106 4 -0.04976366956714173 1106 5 -0.12414313379557029 1106 6 -0.07996276873376473 1106 7 -0.0573941306168706 1106 8 -0.043687496277037496 1106 9 -0.040220882579326656 1106 10-11 -0.1398098677508628 1106 12-13 -0.28455768957775973 1106 14-15 -0.09320565053683083 1106 16-17 -0.03342233724394106 1106 18-19 -0.056048055682907716 1106 20-21 -0.23781863416156312 1106 22-23 -0.1886576618223259 1106 24-25 -0.1070342596628322 1106 26-27 -0.08683948031985977 1106 28-29 -0.1151011632569805 1106 30-31 -0.0984795509836971 1106 32-33 0.022742190023734565 1106 34-35 -0.09180625033929601 1106 36-37 -0.041580202583787695 1106 38-39 -0.06618683591217689 1106 40-41 -0.056701632899226695 1106 42-43 -0.13612231447714862 1106 44-45 -0.012232868792033003 1106 46-47 -0.025831984132899777 1106 48-49 -0.20172382676712175 1106 50-51 0.02047015802470753 1106 52-53 0.1153911635873186 1106 54-55 0.061565386732588934 1106 56-57 0.10418561369139212 1106 58-59 0.04325566832914518 1106 60-61 0.18088783409641707 1106 62-63 0.13193026703343946 1106 64-65 0.1906100377659996 1106 66-67 0.2482240663837061 1106 68-69 0.16464881143972576 1106 70-71 0.10210512333239308 1106 72-73 0.1991289316170608 1106 74-75 0.23544645118493435 1106 76-77 0.3036518977662013 1106 78-79 0.1650104572715172 1106 80-81 0.17576632207908105 1106 82-83 0.03680554461054797 1106 84-85 0.012355883971704174 1106 86-87 0.27301008351481926 1106 88-89 0.20045214240986553 1106 90-91 0.28538519900194714 1106 92-93 0.437266017077949 1106 94-95 0.1577279908173317 1106 96-97 0.26291126939798204 1106 98-99 0.13334308632753178 1106 100-101 -3.476965982081026E-4 1107 1 0.012019849109542236 1107 2 0.10583298367903637 1107 3 0.12974960166951632 1107 4 0.15160089301728163 1107 5 -0.05090250292617071 1107 6 0.05481818202308375 1107 7 0.07931555561425796 1107 8 0.040342368727266376 1107 9 0.0999373163622792 1107 10-11 0.1288835896278684 1107 12-13 0.12225628100409125 1107 14-15 0.19986898118364138 1107 16-17 0.18263827384071618 1107 18-19 0.17055980942087245 1107 20-21 0.14603051551320334 1107 22-23 0.13775298751335896 1107 24-25 0.15124497354836564 1107 26-27 -0.03814818615651916 1107 28-29 0.0858935210754197 1107 30-31 0.20321557301828364 1107 32-33 0.2591776847566223 1107 34-35 0.2564335318233404 1107 36-37 0.28018190214583427 1107 38-39 0.2703301346704663 1107 40-41 0.20655496944731766 1107 42-43 0.1903633368520019 1107 44-45 0.2637207815549871 1107 46-47 0.2431618759670684 1107 48-49 0.23510988907637653 1107 50-51 0.32762266392025907 1107 52-53 0.22487709992405058 1107 54-55 0.23896997038072954 1107 56-57 0.3795274056705935 1107 58-59 0.38568832246021856 1107 60-61 0.22042020061458345 1107 62-63 0.3307356826510599 1107 64-65 0.4118047326776164 1107 66-67 0.5338612635828994 1107 68-69 0.4512164281053259 1107 70-71 0.22752148308301656 1107 72-73 0.3082714377731435 1107 74-75 0.43480727630159777 1107 76-77 0.5011846479787749 1107 78-79 0.415701462479813 1107 80-81 0.4639681927077497 1107 82-83 0.3007312436883396 1107 84-85 0.32482326797322614 1107 86-87 0.525595875926669 1107 88-89 0.500627094428328 1107 90-91 0.7202605733211058 1107 92-93 0.7374816280718228 1107 94-95 0.6497833059717628 1107 96-97 0.8797821388605662 1107 98-99 0.7591312980668441 1107 100-101 1.042675815342502 1108 1 -0.06361860935863461 1108 2 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76-77 -0.39299783184370085 2316 78-79 -0.1825706160050764 2316 80-81 -0.39568044480984454 2316 82-83 -0.3990718474327295 2316 84-85 -0.2950760031975541 2316 86-87 -0.19896015704583192 2316 88-89 -0.11284548893010538 2316 90-91 -0.07001200511973238 2316 92-93 -0.26572198308539186 2316 94-95 -0.15834937905804125 2316 96-97 -0.11259455745512881 2316 98-99 -0.28450829926372023 2316 100-101 -0.48338438836905695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 1.0 4 12.0 5 34.0 6 62.0 7 164.0 8 385.0 9 747.0 10 1330.0 11 2202.0 12 3429.0 13 4992.0 14 6522.0 15 9287.0 16 13053.0 17 19656.0 18 17448.0 19 9447.0 20 7476.0 21 7519.0 22 7653.0 23 8016.0 24 9239.0 25 10900.0 26 13177.0 27 16214.0 28 20289.0 29 25785.0 30 32485.0 31 41563.0 32 53535.0 33 71340.0 34 99729.0 35 146186.0 36 231754.0 37 383390.0 38 352666.0 39 39354.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28338591745743 20.091476570606865 15.255497626662521 21.369639885273184 2 15.640410858242149 21.301978888417665 39.786220784417 23.271389468923193 3 19.263704022742008 25.944499465222037 30.24551797279861 24.546278539237345 4 12.822913811068315 17.577308858118577 37.2558782085928 32.34389912222031 5 13.15682750246569 38.48612614255649 33.815352791689364 14.541693563288453 6 30.84538108403662 37.159360233994605 17.543776510200374 14.4514821717684 7 27.86256176340559 34.071744827830294 20.49114392095724 17.57454948780688 8 26.826658156608662 35.074595977198 19.39009518027986 18.708650685913476 9 26.94465123058918 16.7665938632804 20.25305825210687 36.03569665402355 10-11 24.709081387627343 27.810253700105637 26.668174322619574 20.812490589647442 12-13 25.90314231527054 25.076103580655097 27.853715555424913 21.16703854864945 14-15 22.853764770879284 26.04839575608846 26.728130640587818 24.369708832444438 16-17 21.372222858743633 29.635937079158538 27.249561650031463 21.742278412066366 18-19 21.392618204525725 27.421812342423458 30.615603519276902 20.56996593377391 20-21 22.291513076715894 26.42672942034628 30.828195005899655 20.453562497038174 22-23 21.89330264390394 26.487269075690893 30.637780451205398 20.98164782919977 24-25 21.54625656628309 26.64307793638901 30.904705888704463 20.905959608623434 26-27 21.415650499472857 26.925883656792188 30.767528040515714 20.89093780321924 28-29 21.255548890221956 27.03128374325135 30.85989802039592 20.853269346130773 30-31 21.434833636717286 26.830868919836153 30.871499274607917 20.862798168838644 32-33 21.26859650627727 26.950889806946055 30.63566894034781 21.144844746428866 34-35 21.56387912278418 26.896512215912328 30.655314457240856 20.884294204062634 36-37 21.65721878299261 26.80079662155677 30.53349049812842 21.0084940973222 38-39 21.49344257279815 26.981130038193424 30.598857496606264 20.926569892402167 40-41 21.63028396919823 26.923386083295792 30.542990089661537 20.90333985784444 42-43 21.34684376612313 27.108125457446935 30.30515460206616 21.239876174363772 44-45 21.584154495944023 27.288432779639688 29.934368976999448 21.19304374741684 46-47 21.748666954201052 27.53386677160272 29.343953709763433 21.373512564432797 48-49 21.823709829416927 27.66292731998558 29.09510049806634 21.418262352531155 50-51 22.07004361604682 27.839527019934053 28.86361332343959 21.22681604057954 52-53 22.11609291656369 28.036332574305177 28.510345257833624 21.337229251297508 54-55 21.77479099269547 28.076352976250817 28.616979605254084 21.53187642579963 56-57 21.78648832336461 28.44955781090755 28.398029569652206 21.365924296075637 58-59 21.8656102677369 27.95976958058171 28.628496976989855 21.546123174691537 60-61 21.798275633500435 28.0523584518013 28.843127998190816 21.306237916507452 62-63 21.748876906289986 28.112914632880777 28.97734736933032 21.16086109149892 64-65 21.58325470127717 27.910970716482495 28.946574391723807 21.559200190516524 66-67 21.564269033806486 28.180069308183874 28.687073609802244 21.5685880482074 68-69 21.642521176667483 28.189127241163565 28.679785273000704 21.48856630916825 70-71 21.717654031350044 28.10763583750188 28.558223013508314 21.616487117639753 72-73 21.660337111071254 28.180519205517303 28.344581766440896 21.814561916970547 74-75 21.627164681019792 28.42982231454782 28.160393798135264 21.782619206297124 76-77 21.721943052595396 28.284745421094904 28.294103285630218 21.699208240679475 78-79 21.739549034910233 28.234176960817543 28.16933175849271 21.85694224577951 80-81 21.71522458574953 28.482820220425697 28.047409581133586 21.754545612691185 82-83 21.674036002670597 28.63246478945054 27.9712640885688 21.72223511931006 84-85 21.647172585974758 28.356231642286694 27.960021463134304 22.03657430860424 86-87 21.602420327681223 28.253462559843847 28.070774249316305 22.073342863158626 88-89 21.730791033486156 28.086520655986302 28.0802220933183 22.102466217209233 90-91 21.674271026773088 28.071022153779147 28.074861578249422 22.179845241198347 92-93 21.429674773703912 27.947727921003402 28.253995105603842 22.368602199688844 94-95 21.338630524514308 27.975456000940586 28.29497308714151 22.390940387403592 96-97 21.336830935180593 28.05598762362429 28.192246529342 22.414934911853116 98-99 21.133626990901735 28.129699625859544 27.935160984774637 22.801512398464084 100-101 21.05541967156269 28.540257957612592 27.831711424394207 22.572610946430512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 591.0 1 428.5 2 331.0 3 414.5 4 402.5 5 415.5 6 654.5 7 1301.0 8 2194.5 9 2861.5 10 2934.5 11 2813.5 12 2922.5 13 3156.5 14 3448.0 15 3610.5 16 3702.5 17 3831.5 18 4010.0 19 4255.0 20 4614.5 21 5063.5 22 5656.5 23 6518.5 24 7574.0 25 9069.5 26 11207.5 27 14136.0 28 16880.0 29 20076.0 30 23949.0 31 26408.0 32 28821.5 33 31791.5 34 35980.0 35 39855.0 36 44106.5 37 48002.5 38 49528.0 39 51088.0 40 52456.5 41 54019.5 42 55459.5 43 57779.0 44 60205.5 45 60545.0 46 59805.0 47 60501.5 48 64001.0 49 63789.5 50 60254.0 51 57073.0 52 53696.5 53 51394.5 54 49721.0 55 46266.5 56 40281.0 57 35279.5 58 32077.5 59 28516.5 60 23822.0 61 19133.5 62 15070.5 63 11450.5 64 8278.5 65 6029.0 66 4318.5 67 3332.5 68 2525.0 69 1661.0 70 1109.0 71 757.5 72 589.5 73 489.0 74 376.5 75 267.0 76 172.5 77 86.5 78 54.0 79 29.0 80 18.0 81 14.0 82 10.0 83 7.0 84 3.5 85 1.5 86 2.0 87 2.0 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04966866561050768 2 0.0 3 0.0 4 0.0 5 0.010257659202170066 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004528966489847017 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014096783114093364 24-25 4.798904889904124E-4 26-27 0.002369459289390161 28-29 0.002819356622818673 30-31 0.0010497604446665272 32-33 0.0 34-35 0.0 36-37 4.199041778666108E-4 38-39 3.599178667428093E-4 40-41 0.0 42-43 0.01121744018015089 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.7995893337140466E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.399452444952062E-4 84-85 1.4996577780950388E-4 86-87 0.0 88-89 0.0 90-91 0.007828213601656102 92-93 0.0023094729782663598 94-95 0.0 96-97 0.0 98-99 0.0015596440892188404 100-101 0.011037481246779484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1667047.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.6100273264775 #Duplication Level Percentage of deduplicated Percentage of total 1 77.95461649116614 44.130129697899875 2 12.409778510390002 14.050358011774225 3 3.995253154070999 6.78514170684464 4 1.7845416182648455 4.040917991008372 5 0.9720482592639489 2.751383925979352 6 0.6148135190031433 2.088276606687344 7 0.41379626563943056 1.6397512533809753 8 0.2801431709513216 1.2687130050304296 9 0.2199193701545252 1.1204677398662453 >10 1.2272197734370138 12.761619989975573 >50 0.08865485534895738 3.4020631103226617 >100 0.036983452097444004 3.564902284008885 >500 0.0013814420361442953 0.48744825298803207 >1k 5.313238600554983E-4 0.4203737371196308 >5k 2.1252954402219926E-4 0.8586741161484844 >10k+ 1.0626477201109963E-4 0.6297785709652854 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10469 0.6279966911550784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8715 0.5227807014439305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5559 0.3334639035372128 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2298 0.13784854296249596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9993155561900776E-5 0.0 16-17 0.0 0.0 0.0 1.1997262224760309E-4 0.0 18-19 0.0 0.0 0.0 1.7995893337140463E-4 0.0 20-21 0.0 0.0 0.0 2.0995208893330542E-4 0.0 22-23 0.0 0.0 0.0 3.5991786674280927E-4 0.0 24-25 0.0 0.0 0.0 5.398768001142139E-4 0.0 26-27 0.0 0.0 0.0 9.597809779808247E-4 0.0 28-29 0.0 0.0 0.0 0.002129514044894955 0.0 30-31 0.0 0.0 0.0 0.0046489391120946195 0.0 32-33 0.0 0.0 0.0 0.007588268357160896 0.0 34-35 0.0 0.0 0.0 0.010497604446665271 0.0 36-37 0.0 0.0 0.0 0.014216755736340967 0.0 38-39 0.0 0.0 0.0 0.017336043914778648 0.0 40-41 0.0 0.0 0.0 0.022704818760358885 0.0 42-43 0.0 0.0 0.0 0.030682998139824492 0.0 44-45 0.0 0.0 0.0 0.04376001396481323 0.0 46-47 0.0 0.0 0.0 0.06937416881467649 0.0 48-49 0.0 0.0 0.0 0.12525141762649764 0.0 50-51 0.0 0.0 0.0 0.1663120475907398 0.0 52-53 0.0 0.0 0.0 0.2053331429767727 0.0 54-55 0.0 0.0 0.0 0.2585110077880228 0.0 56-57 0.0 0.0 0.0 0.2955525549069702 0.0 58-59 0.0 0.0 0.0 0.3477106524291157 0.0 60-61 0.0 0.0 0.0 0.4334010978694662 0.0 62-63 0.0 0.0 0.0 0.49089797708162997 0.0 64-65 0.0 0.0 0.0 0.5613818926520968 0.0 66-67 0.0 0.0 0.0 0.7033095047710112 0.0 68-69 0.0 0.0 0.0 0.8475165967126301 0.0 70-71 0.0 0.0 0.0 0.9773569671400986 0.0 72-73 0.0 0.0 0.0 1.1442088915309525 5.998631112380155E-5 74-75 0.0 0.0 0.0 1.2928849636512947 5.998631112380155E-5 76-77 0.0 0.0 0.0 1.4132774900767644 5.998631112380155E-5 78-79 0.0 0.0 0.0 1.5813291406900944 5.998631112380155E-5 80-81 0.0 0.0 0.0 1.8193248300737772 5.998631112380155E-5 82-83 0.0 0.0 0.0 2.0354255158972725 5.998631112380155E-5 84-85 0.0 0.0 0.0 2.285088542794534 5.998631112380155E-5 86-87 0.0 0.0 0.0 2.7442537612916733 5.998631112380155E-5 88-89 0.0 0.0 0.0 3.3906362568061965 5.998631112380155E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6330 0.0 41.965984 1 GTATCAA 10500 0.0 36.84041 1 ATCAACG 13535 0.0 28.394632 3 TATCAAC 13640 0.0 28.21088 2 TCAACGC 13715 0.0 27.987335 4 CAACGCA 14065 0.0 27.290886 5 AACGCAG 14365 0.0 26.78226 6 TATCACG 120 1.7112592E-4 23.750015 7 GTACATG 9720 0.0 23.467363 1 ACGCAGA 16420 0.0 23.373947 7 CGCAGAG 16600 0.0 22.94881 8 TACATGG 9995 0.0 22.481451 2 AGTACTT 9450 0.0 22.418001 12-13 GAGTACT 9125 0.0 22.201384 12-13 GACCGGT 130 2.9422657E-4 21.92309 7 ACATGGG 10050 0.0 21.554838 3 GCAGAGT 17395 0.0 21.490385 9 GTACTTT 9500 0.0 21.023752 14-15 GTACCTG 1595 0.0 20.855793 1 CATGGGG 3600 0.0 20.189934 4 >>END_MODULE