FastQCFastQC Report
Thu 26 May 2016
SRR937397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833917
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111150.6060797735121055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85820.4679601094269806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54390.2965783075242773No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24280.13239421413291877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.12001633661719696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59300.046.4171831
GTATCAA105000.039.5257231
TATCAAC129050.031.999162
ATCAACG128850.031.975073
TCAACGC130350.031.5741144
CAACGCA132250.031.084565
AACGCAG136800.030.1392546
ACGCAGA155100.026.4628357
CGCAGAG155450.026.2191838
TACCTGG16250.026.0263632
GTACATG102550.025.6820091
TACATGG103750.024.7791272
GCAGAGT163900.024.2291439
GAGTACT87100.023.80417312-13
GTACCTG18450.023.7053491
ACATGGG105000.023.307453
GTACTTT92150.022.34410514-15
CATGGGA62350.021.799874
AGTACTT89700.020.54595212-13
CAGAGTA159550.020.51290710-11
Produced by FastQC (version 0.11.5)