##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937397_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1833917 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.680532434128697 33.0 31.0 34.0 30.0 34.0 2 31.764260323667866 33.0 31.0 34.0 30.0 34.0 3 31.79843962403969 33.0 31.0 34.0 30.0 34.0 4 35.43711138508449 37.0 35.0 37.0 33.0 37.0 5 35.43091808407905 37.0 35.0 37.0 33.0 37.0 6 35.49883718837875 37.0 35.0 37.0 33.0 37.0 7 35.439291418313914 37.0 35.0 37.0 33.0 37.0 8 35.428600640050774 37.0 35.0 37.0 33.0 37.0 9 37.03300203880546 39.0 37.0 39.0 33.0 39.0 10-11 36.943980289184296 39.0 37.0 39.0 33.0 39.0 12-13 36.83011199525387 39.0 37.0 39.0 33.0 39.0 14-15 37.96105194509893 40.0 38.0 41.0 33.0 41.0 16-17 37.90877313422581 40.0 38.0 41.0 33.0 41.0 18-19 37.89692963203896 40.0 38.0 41.0 33.0 41.0 20-21 37.840856211049896 40.0 38.0 41.0 33.0 41.0 22-23 37.71086423213264 40.0 37.5 41.0 32.0 41.0 24-25 37.56722632485548 40.0 37.0 41.0 32.0 41.0 26-27 37.39599474785391 40.0 37.0 41.0 32.0 41.0 28-29 37.21044845540992 39.0 36.5 41.0 31.5 41.0 30-31 37.173950620447926 39.0 36.5 41.0 31.5 41.0 32-33 37.00408878918729 39.0 36.0 41.0 31.0 41.0 34-35 36.798377189371166 39.0 36.0 41.0 30.5 41.0 36-37 36.80676688203447 39.0 36.0 41.0 30.5 41.0 38-39 36.77634756643839 39.0 36.0 41.0 30.5 41.0 40-41 36.60299048430218 39.0 35.5 41.0 30.0 41.0 42-43 36.34720927937306 39.0 35.0 41.0 30.0 41.0 44-45 36.086108586157394 39.0 35.0 41.0 29.5 41.0 46-47 35.70029914112798 39.0 35.0 41.0 27.5 41.0 48-49 35.34419278516967 39.0 35.0 41.0 26.0 41.0 50-51 34.40245223747858 38.0 34.0 39.5 24.5 40.5 52-53 34.52740200347125 38.0 34.5 39.5 24.5 40.5 54-55 34.88223703689971 39.0 35.0 40.0 25.0 41.0 56-57 34.67667129973712 38.5 34.0 40.0 23.5 41.0 58-59 34.441680839427306 38.0 34.0 40.0 23.0 41.0 60-61 34.16963826607202 38.0 34.0 40.0 22.5 41.0 62-63 33.861068412583556 37.5 34.0 40.0 22.0 41.0 64-65 33.50842486328443 37.0 33.0 40.0 21.0 41.0 66-67 33.1290131996159 36.5 33.0 39.5 19.5 41.0 68-69 32.64908853563166 36.0 32.0 39.0 18.0 41.0 70-71 32.23005348660817 35.5 32.0 39.0 15.5 40.0 72-73 31.825364234041125 35.0 31.5 38.0 12.0 40.0 74-75 31.379753282182346 35.0 31.0 37.0 8.0 39.5 76-77 30.943420830931828 35.0 31.0 37.0 7.0 39.0 78-79 30.47624810719351 35.0 30.5 36.0 3.5 39.0 80-81 30.036564904518578 34.0 30.0 36.0 2.0 37.5 82-83 29.60828870663176 34.0 29.5 35.5 2.0 37.0 84-85 29.15637539757797 34.0 29.0 35.0 2.0 37.0 86-87 28.733360888197232 34.0 29.0 35.0 2.0 36.0 88-89 28.362920731963335 34.0 29.0 35.0 2.0 36.0 90-91 27.937746364748243 34.0 27.5 35.0 2.0 35.5 92-93 27.51389266798879 34.0 26.5 35.0 2.0 35.0 94-95 27.005424727509478 33.0 25.5 35.0 2.0 35.0 96-97 26.417155465596316 33.0 25.0 35.0 2.0 35.0 98-99 25.736688192540882 33.0 23.5 35.0 2.0 35.0 100-101 23.9517982547738 30.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01711067692949797 1101 2 -0.014626943080394028 1101 3 0.07085965432423436 1101 4 -0.049839449438863426 1101 5 -0.009601682938502165 1101 6 -0.017401852935542195 1101 7 -7.119703151090562E-4 1101 8 0.012948100061905166 1101 9 0.006217782002728711 1101 10-11 0.023399704916705844 1101 12-13 0.03729099124775104 1101 14-15 0.01946637440019572 1101 16-17 0.07310089229196848 1101 18-19 0.18898957149363582 1101 20-21 0.17375955016555622 1101 22-23 -3.665705277242637E-4 1101 24-25 0.1203435510329669 1101 26-27 0.20705833587897615 1101 28-29 0.1363264274282443 1101 30-31 0.082854645055086 1101 32-33 0.022554215068929295 1101 34-35 -0.056842161671781355 1101 36-37 -0.010949875311005997 1101 38-39 0.10461340569410993 1101 40-41 0.06847001345746406 1101 42-43 0.16480037485789012 1101 44-45 0.022168767369535658 1101 46-47 0.25795862624137555 1101 48-49 0.29063155658382556 1101 50-51 0.2339617644093579 1101 52-53 0.32133862384905143 1101 54-55 0.2841664371212289 1101 56-57 0.4797320159755927 1101 58-59 0.35493979056054314 1101 60-61 0.47694525622356565 1101 62-63 0.3452304278502396 1101 64-65 0.14539868669691458 1101 66-67 0.18236293252761016 1101 68-69 0.3410387729361588 1101 70-71 0.3458950293389549 1101 72-73 0.31819567042262875 1101 74-75 0.4119966287722967 1101 76-77 0.3546542005809883 1101 78-79 0.3532497049885386 1101 80-81 0.44059301964719566 1101 82-83 0.5812869389685318 1101 84-85 0.29189369576306845 1101 86-87 0.5731919671075509 1101 88-89 0.6416703182630812 1101 90-91 0.6656248396890732 1101 92-93 0.5105501425625505 1101 94-95 0.5506730028614122 1101 96-97 0.6119960808315419 1101 98-99 0.6327181284900121 1101 100-101 1.223895730738196 1102 1 0.10097368659253547 1102 2 0.07536618034594156 1102 3 0.05531028215806799 1102 4 -0.10090645749139782 1102 5 0.06366572236812118 1102 6 0.03916166765753104 1102 7 0.11218952229247492 1102 8 0.18964851421314677 1102 9 0.2243219284893172 1102 10-11 0.04617688380520946 1102 12-13 0.17763441743697683 1102 14-15 0.27376803005586936 1102 16-17 0.41777965453908195 1102 18-19 0.23622510154608278 1102 20-21 0.24096154030900863 1102 22-23 0.26898912375750683 1102 24-25 0.35612639635485976 1102 26-27 0.39316742924583536 1102 28-29 0.3173891561331672 1102 30-31 0.3017835247279592 1102 32-33 0.24733283382310844 1102 34-35 0.2830502433025899 1102 36-37 0.17519424643358406 1102 38-39 0.3889004918229091 1102 40-41 0.42158548863401535 1102 42-43 0.37022671672954033 1102 44-45 0.37146880735314625 1102 46-47 0.46762283225152856 1102 48-49 0.5315007311937379 1102 50-51 0.4639395816686829 1102 52-53 0.2803324493617865 1102 54-55 0.48865593601228596 1102 56-57 0.49064814949579727 1102 58-59 0.2511388413579567 1102 60-61 0.23563618594315727 1102 62-63 0.402586690943167 1102 64-65 0.4920460627581562 1102 66-67 0.5001677145371843 1102 68-69 0.3379032110259246 1102 70-71 0.3822224947386914 1102 72-73 0.30264117215240205 1102 74-75 0.3145266079375304 1102 76-77 0.21216939188528983 1102 78-79 0.29228362043212286 1102 80-81 0.3259903249871883 1102 82-83 0.4700580751296286 1102 84-85 0.35469445093312757 1102 86-87 0.5158842121474727 1102 88-89 0.5491656886316889 1102 90-91 0.44409038030888937 1102 92-93 0.2508640841606571 1102 94-95 0.4251067107547364 1102 96-97 0.7162736627837099 1102 98-99 0.8494149695568645 1102 100-101 0.9695604706225893 1103 1 0.06573448033250884 1103 2 -0.004879856235422864 1103 3 0.13707778237019852 1103 4 0.10168375399478435 1103 5 0.056543806766235605 1103 6 0.07741244695692728 1103 7 0.21768668169793415 1103 8 0.17431492782510816 1103 9 0.20868812542401116 1103 10-11 0.1842966416487144 1103 12-13 0.20606015525571308 1103 14-15 0.239268489493206 1103 16-17 0.2702950764072014 1103 18-19 0.1706687140149512 1103 20-21 0.23310084084726412 1103 22-23 0.24325602774950283 1103 24-25 0.10853464557233394 1103 26-27 0.022115097285364982 1103 28-29 -0.15960311563895857 1103 30-31 -0.17431142839846103 1103 32-33 0.04888137966327122 1103 34-35 0.08605872011251137 1103 36-37 0.2536500549110272 1103 38-39 0.15526999231683902 1103 40-41 0.30328493058765105 1103 42-43 0.19291555292156914 1103 44-45 0.06071084058822862 1103 46-47 0.2186562968088097 1103 48-49 0.2536377526719704 1103 50-51 0.16196314867785588 1103 52-53 0.0692391687871563 1103 54-55 0.10771369852403723 1103 56-57 0.25790713007989297 1103 58-59 0.28895350302336453 1103 60-61 0.23414539066094875 1103 62-63 0.28091134078999147 1103 64-65 0.35307876109663994 1103 66-67 0.3442017213265487 1103 68-69 0.35515949710654837 1103 70-71 0.35748714886928923 1103 72-73 0.3857888460316481 1103 74-75 0.4379834658015902 1103 76-77 0.3578613903465815 1103 78-79 0.5695984879575953 1103 80-81 0.5552111470909793 1103 82-83 0.44367849425327677 1103 84-85 0.4491153394294791 1103 86-87 0.5943176807416144 1103 88-89 0.6027652415204834 1103 90-91 0.6707080181170646 1103 92-93 0.7603997849598549 1103 94-95 0.7734176294118917 1103 96-97 0.9748790740129465 1103 98-99 1.0987712771739204 1103 100-101 1.1489728572256332 1104 1 0.0804799457465677 1104 2 0.09372504511236457 1104 3 0.11602026362966456 1104 4 0.0626857750375791 1104 5 0.11080689176684189 1104 6 0.07481502906240678 1104 7 0.09665517443581706 1104 8 0.03880592245496928 1104 9 0.053673652244789594 1104 10-11 0.0732687563622818 1104 12-13 0.30431611016653193 1104 14-15 0.2527238837339638 1104 16-17 0.1813112810674795 1104 18-19 0.1285856755601742 1104 20-21 0.18090238013784443 1104 22-23 0.17401258877685422 1104 24-25 0.2701830273148289 1104 26-27 0.23458549082430125 1104 28-29 0.16993218193726278 1104 30-31 0.08385563596571899 1104 32-33 0.06553206317620663 1104 34-35 0.09627730487213881 1104 36-37 0.05590549548167445 1104 38-39 0.172829899696346 1104 40-41 0.01614441658120569 1104 42-43 0.1762685011999494 1104 44-45 0.31018683199364716 1104 46-47 0.16641170253388538 1104 48-49 0.18762287803257038 1104 50-51 -0.028248879226083545 1104 52-53 0.06398239623251101 1104 54-55 0.17844283461904098 1104 56-57 0.14085655689488163 1104 58-59 -0.026883561075791818 1104 60-61 0.06533619614318553 1104 62-63 0.15764961918348064 1104 64-65 0.18182929727664288 1104 66-67 0.28149393623829866 1104 68-69 0.15414779783064603 1104 70-71 -0.023557059846815775 1104 72-73 0.12048864903151113 1104 74-75 0.24899221056616483 1104 76-77 0.24944554827650123 1104 78-79 0.14825445032831297 1104 80-81 0.09506809377529635 1104 82-83 0.1804683440836108 1104 84-85 0.16585338224731672 1104 86-87 0.26666679208154065 1104 88-89 0.22140467433283462 1104 90-91 0.3011331045684251 1104 92-93 0.19588533092115412 1104 94-95 0.1815149535669356 1104 96-97 0.11290834625104296 1104 98-99 0.34529118675213155 1104 100-101 0.37289350356670425 1105 1 -0.08005657497335861 1105 2 -0.06474533265591376 1105 3 -0.16740273090557523 1105 4 -0.2088330197797248 1105 5 -0.0017654593987543876 1105 6 -0.0171326930325435 1105 7 0.0192376723139418 1105 8 0.0031094899710240043 1105 9 -0.005541243214288727 1105 10-11 0.10829416568747519 1105 12-13 0.02774927268623628 1105 14-15 0.01411702523699887 1105 16-17 -0.07423112134220133 1105 18-19 -0.006591189935264197 1105 20-21 -0.1363171440940789 1105 22-23 -0.08684418090979307 1105 24-25 0.05136949070689667 1105 26-27 -0.002433098718839233 1105 28-29 0.029621654244394335 1105 30-31 -0.09034363525781686 1105 32-33 -0.19191300737215755 1105 34-35 0.018029559165348985 1105 36-37 -0.05176410033030976 1105 38-39 -0.07082542690229587 1105 40-41 0.11793732154033165 1105 42-43 -0.13581555272015322 1105 44-45 0.048451212352844664 1105 46-47 0.0038641331714259763 1105 48-49 0.02434982936026131 1105 50-51 -0.035974420224548 1105 52-53 -0.04093184596548127 1105 54-55 0.08752127062135884 1105 56-57 -0.10226367420341376 1105 58-59 -0.11244108783056816 1105 60-61 0.09862102946694762 1105 62-63 0.113370174040611 1105 64-65 0.0689707322387747 1105 66-67 0.1412842375126928 1105 68-69 0.0799889432817551 1105 70-71 -0.011299533304757858 1105 72-73 0.17996635923544346 1105 74-75 0.12591248123025522 1105 76-77 0.07160952085272498 1105 78-79 0.18452926669834113 1105 80-81 0.15972201004471032 1105 82-83 0.26232327800234856 1105 84-85 0.18880728809348213 1105 86-87 0.1415489987468881 1105 88-89 0.22423267160151994 1105 90-91 0.2249454393120871 1105 92-93 0.21776574861685205 1105 94-95 0.19398902839734333 1105 96-97 0.1514997825958453 1105 98-99 0.20901700854012262 1105 100-101 0.3697887584363819 1106 1 -0.03796064979956881 1106 2 -0.03922628146017004 1106 3 -0.11714200755669424 1106 4 -0.03355181875228652 1106 5 0.009136393019772981 1106 6 -0.07862291165687196 1106 7 -0.09391502471223134 1106 8 -0.10814535126128533 1106 9 -0.07106981459870099 1106 10-11 -0.17154219834228712 1106 12-13 -0.31337716963779627 1106 14-15 -0.18084670222596344 1106 16-17 -0.18985814013220903 1106 18-19 -0.13863357950262412 1106 20-21 0.007147926395496995 1106 22-23 0.0225197592119315 1106 24-25 0.051254352061413044 1106 26-27 0.11033322701379689 1106 28-29 0.1960085880945428 1106 30-31 0.22600095229855555 1106 32-33 0.36715775421203034 1106 34-35 0.1477923581008227 1106 36-37 -0.04214422786375138 1106 38-39 -0.027968350280225707 1106 40-41 0.12722105098151104 1106 42-43 0.0456182247856205 1106 44-45 0.13517920387121762 1106 46-47 0.11419786523993736 1106 48-49 0.13084225802838745 1106 50-51 0.095489957887672 1106 52-53 0.21259804217230993 1106 54-55 0.296271498580424 1106 56-57 0.24820754422618307 1106 58-59 0.2417246129078663 1106 60-61 0.2945259574009782 1106 62-63 0.4731676227536852 1106 64-65 0.4108065522577462 1106 66-67 0.41016774811317447 1106 68-69 0.41480362415452277 1106 70-71 0.3595465889183984 1106 72-73 0.47830140805732313 1106 74-75 0.5176326989582059 1106 76-77 0.6958618923997228 1106 78-79 0.6073500553285136 1106 80-81 0.6987571018310774 1106 82-83 0.5691770411041972 1106 84-85 0.7102717589348124 1106 86-87 0.5450404043038368 1106 88-89 0.6911191062599507 1106 90-91 0.706908854587045 1106 92-93 0.6880561143713742 1106 94-95 0.7213472069503375 1106 96-97 0.6866379335155628 1106 98-99 0.6965202154218595 1106 100-101 0.7867328396918118 1107 1 0.1545429312922657 1107 2 0.18584312982601503 1107 3 0.1808313597306963 1107 4 0.03468345456018085 1107 5 0.10355932143919944 1107 6 0.23909509283496533 1107 7 0.12546885064809032 1107 8 0.23939740078568406 1107 9 0.33278597498046736 1107 10-11 0.33190773416223607 1107 12-13 0.3658616346606891 1107 14-15 0.3182499194633692 1107 16-17 0.295945704457246 1107 18-19 0.26664285720784164 1107 20-21 0.32059809469208034 1107 22-23 0.27478188923945623 1107 24-25 0.4568240551402667 1107 26-27 0.30271449097710956 1107 28-29 0.20587943299642575 1107 30-31 0.22659190613636326 1107 32-33 0.2652557965484874 1107 34-35 0.2695774808011109 1107 36-37 0.2601131863226769 1107 38-39 0.3229860180623305 1107 40-41 0.15176847760597667 1107 42-43 0.1385040837466036 1107 44-45 0.2934516733803605 1107 46-47 0.14266386107820495 1107 48-49 0.16515589683033483 1107 50-51 0.2566258271645552 1107 52-53 0.23359090356784407 1107 54-55 0.22561640930365456 1107 56-57 0.21638593270538564 1107 58-59 0.25030449630869356 1107 60-61 0.22201547939153699 1107 62-63 0.34566454271996605 1107 64-65 0.36380435349683893 1107 66-67 0.43437769735893994 1107 68-69 0.45818199377035995 1107 70-71 0.5353781980223218 1107 72-73 0.37256930320059567 1107 74-75 0.47085892375056204 1107 76-77 0.38055928277115214 1107 78-79 0.5627779884343092 1107 80-81 0.5302880996441033 1107 82-83 0.592154976862652 1107 84-85 0.6085171713056425 1107 86-87 0.6150264336245783 1107 88-89 0.5911005376356187 1107 90-91 0.8452244740422472 1107 92-93 0.9058071297509116 1107 94-95 0.8613811610062534 1107 96-97 0.9993350409365256 1107 98-99 0.8741187912378514 1107 100-101 1.238164097465578 1108 1 -0.18396783503612468 1108 2 -0.11961809838111392 1108 3 -0.11995253306139375 1108 4 -0.06838625009763888 1108 5 -0.01127988837951932 1108 6 -0.04718027796909041 1108 7 -0.16067560030169403 1108 8 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-0.4230441236799649 2316 56-57 -0.6877561702207728 2316 58-59 -0.7098521257196069 2316 60-61 -0.49558063153863685 2316 62-63 -0.5192206675674811 2316 64-65 -0.43805637318204305 2316 66-67 -0.6643965425508966 2316 68-69 -0.7043947849718819 2316 70-71 -0.8496410939560377 2316 72-73 -0.9290139774294808 2316 74-75 -1.1194063962400875 2316 76-77 -1.0128813297010524 2316 78-79 -0.7606540249336504 2316 80-81 -0.9046453448974177 2316 82-83 -0.7649712220288265 2316 84-85 -1.127390091634517 2316 86-87 -1.0129201381934543 2316 88-89 -0.9559394921424413 2316 90-91 -1.102595563996008 2316 92-93 -0.8549407453167994 2316 94-95 -0.7029173916214688 2316 96-97 -0.6303965486799825 2316 98-99 -0.964541448790321 2316 100-101 -1.2661704944273673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6687.0 3 1334.0 4 1210.0 5 1429.0 6 1566.0 7 1868.0 8 2268.0 9 2778.0 10 3766.0 11 4955.0 12 6817.0 13 8625.0 14 10166.0 15 13066.0 16 16611.0 17 22481.0 18 19026.0 19 12106.0 20 11506.0 21 11936.0 22 12764.0 23 14014.0 24 15650.0 25 17677.0 26 20694.0 27 24171.0 28 28633.0 29 34985.0 30 42581.0 31 52831.0 32 66856.0 33 88003.0 34 119844.0 35 168500.0 36 249064.0 37 360386.0 38 311620.0 39 45432.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20017202659467 19.08691389429168 14.882979811208195 21.82993426790545 2 15.101035639218175 20.319574920529927 41.136770522138185 23.442618918113713 3 18.996218654383263 25.640442521851938 30.197464196938295 25.165874626826508 4 12.181789854339609 16.750535053888058 38.049697827804216 33.01797726396812 5 13.047119182528483 37.8021543133823 34.35302271835299 14.797703785736227 6 30.522393115324437 36.57259897376725 18.2821200360143 14.622887874894012 7 27.790250221690176 33.56168013563123 20.980332270276797 17.6677373724018 8 26.530032447576595 34.427976924468005 20.065485208461613 18.976505419493787 9 26.795497706457105 16.561204486995972 20.629811905443532 36.013485901103394 10-11 24.423085486918936 27.17942371248625 27.41396790583044 20.983522894764377 12-13 25.648802823827687 24.67723780694373 28.240407837651766 21.433551531576818 14-15 22.747629439448783 25.532233969061224 27.208596489934152 24.51154010155584 16-17 21.252723998973917 29.08049377697534 27.68905174375546 21.97773048029528 18-19 21.266456502006726 27.021073008864665 30.967792689252676 20.744677799875934 20-21 22.17403537526463 26.013139384006013 31.056668715427165 20.756156525302192 22-23 21.74786343230865 26.037224874056825 30.916105811387634 21.29880588224689 24-25 21.392841993014567 26.186443386277205 31.18523198418376 21.23548263652447 26-27 21.22066746014987 26.46281961169223 30.984881659091972 21.33163126906593 28-29 21.083284687703248 26.492442012451278 31.207658156117095 21.216615143728372 30-31 21.259619245867516 26.305308102703634 31.192035250762846 21.243037400666008 32-33 21.04779338949764 26.41772358341012 30.95032493582282 21.58415809126942 34-35 21.461854024384447 26.393976494035005 30.896855327357684 21.247314154222863 36-37 21.436789709868915 26.32958991790928 30.823737143618875 21.409883228602926 38-39 21.305219512328364 26.488758750856988 30.86851658121806 21.33750515559659 40-41 21.47033967055065 26.423716026525955 30.783842089823104 21.32210221310029 42-43 21.20879883270757 26.4988984251276 30.628561408224037 21.6637413339408 44-45 21.367308432502043 26.70068765893559 30.355171619706827 21.576832288855545 46-47 21.51400777593677 26.916956338612163 29.739243351202582 21.829792534248487 48-49 21.539027197835665 27.001239617723527 29.64019399006667 21.819539194374133 50-51 21.764718737707067 27.218956248087768 29.368525722278072 21.647799291927093 52-53 21.733363893429043 27.36828676151406 29.17427368095533 21.724075664101566 54-55 21.533842071516755 27.38804659235297 29.199883401645 21.878227934485274 56-57 21.601130769073194 27.700209629290747 28.99791736372389 21.700742237912173 58-59 21.657704061985356 27.262058549614775 29.18956933068597 21.890668057713896 60-61 21.60440196139216 27.24333613654471 29.47386936306649 21.67839253899664 62-63 21.583202458176853 27.27300785250939 29.585606008876752 21.55818368043701 64-65 21.392212735402396 27.164858083263027 29.573104032761805 21.869825148572776 66-67 21.41495866515704 27.386788419961462 29.262528493248347 21.935724421633154 68-69 21.38902052148358 27.418242502290862 29.311430276528593 21.881306699696964 70-71 21.462138724297606 27.367567409587696 29.192133815921984 21.978160050192717 72-73 21.442282555516794 27.363791846400414 29.067081810530603 22.126843787552186 74-75 21.349139255546525 27.633249796849285 28.881435097614773 22.136175849989414 76-77 21.5374756654627 27.412919437009364 28.8989822037947 22.150622693733236 78-79 21.493966478432665 27.45065208139969 28.891251298126274 22.164130142041373 80-81 21.402335970249208 27.7070357693396 28.781639986147177 22.108988274264014 82-83 21.481897300575287 27.66462338627287 28.82281918061178 22.03066013254006 84-85 21.493225584340635 27.408832099862646 28.735419649970545 22.362522665826177 86-87 21.531461552453948 27.41747115662468 28.768976048568195 22.28209124235318 88-89 21.63799602307491 27.100504763569617 28.91178109430994 22.34971811904554 90-91 21.556570572655556 27.08608911568509 29.017182971566196 22.340157340093153 92-93 21.415024891250162 26.994898830213675 29.105949998975667 22.484126279560492 94-95 21.2261842067885 27.003187093418873 29.170918673941877 22.599710025850747 96-97 21.234470586500123 27.068854099579436 29.050757686250538 22.645917627669903 98-99 21.143263574418082 27.17199839503236 28.908482701939537 22.776255328610024 100-101 21.16290572393607 27.07944313849517 29.185248790847556 22.5724023467212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2474.0 1 1475.5 2 509.5 3 558.0 4 486.0 5 474.5 6 784.0 7 1567.5 8 2732.0 9 3429.5 10 3260.0 11 2985.0 12 3077.5 13 3341.5 14 3483.0 15 3588.0 16 3698.0 17 3851.0 18 4081.5 19 4332.5 20 4774.5 21 5318.0 22 6050.5 23 7041.0 24 8185.5 25 9821.5 26 12285.5 27 15575.5 28 18442.5 29 21750.5 30 25628.5 31 28658.5 32 31802.5 33 35246.0 34 39451.5 35 43540.0 36 47948.0 37 51911.0 38 54244.0 39 55733.0 40 57003.5 41 59211.5 42 61131.0 43 63354.5 44 65729.5 45 66628.0 46 66357.5 47 67087.0 48 70396.0 49 70161.0 50 66265.5 51 62895.5 52 59661.5 53 57287.5 54 55135.5 55 50516.5 56 44284.5 57 39116.5 58 35332.5 59 31346.0 60 26350.0 61 21619.0 62 17037.5 63 12847.0 64 9521.5 65 7081.0 66 5080.0 67 3728.5 68 2716.0 69 1872.5 70 1328.0 71 945.0 72 697.5 73 526.0 74 387.5 75 282.5 76 210.0 77 128.0 78 78.0 79 55.0 80 36.5 81 29.5 82 21.5 83 13.0 84 11.0 85 9.0 86 9.5 87 7.5 88 5.5 89 3.5 90 3.0 91 5.0 92 4.0 93 2.0 94 0.5 95 0.5 96 1.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2797291262363564 2 0.25148357313880615 3 0.2548643150153469 4 0.25420997787795196 5 0.2437951117744151 6 0.19264775886804036 7 0.19957282690547065 8 0.1952105793228374 9 0.194174545521962 10-11 0.2056527094737657 12-13 0.20595261399507175 14-15 0.19060295531368104 16-17 0.19943650666851337 18-19 0.1879856067641011 20-21 0.19313851172108662 22-23 0.1923751183941258 24-25 0.18504108964582366 26-27 0.195783124318058 28-29 0.19098465197716147 30-31 0.19641019740806154 32-33 0.19807330429894046 34-35 0.1901939946028092 36-37 0.19101191602455292 38-39 0.18441401655582015 40-41 0.18583174702017594 42-43 0.18299628609146432 44-45 0.1950197309910972 46-47 0.18528646607234678 48-49 0.19161172506716498 50-51 0.19613755693414697 52-53 0.19867311334155255 54-55 0.20189027093374454 56-57 0.18018808921014418 58-59 0.17241783570357871 60-61 0.1787703587457884 62-63 0.17950648802535774 64-65 0.17713451590230092 66-67 0.18754938200583776 68-69 0.17855224636665673 70-71 0.18389599965538242 72-73 0.19343841624239264 74-75 0.18427769631886284 76-77 0.18771296629018652 78-79 0.18506835369321512 80-81 0.1768346113809949 82-83 0.19232059029934287 84-85 0.17680734733360343 86-87 0.17743442042360696 88-89 0.18305081418624725 90-91 0.17975186445188088 92-93 0.1883673034275815 94-95 0.18588627511495884 96-97 0.17874309469839694 98-99 0.1826963815701583 100-101 0.17408094259445767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1833917.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.56456029356002 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43132691983044 45.14864847380496 2 12.390164567829657 14.264687506239229 3 3.8141120228429757 6.586730445160099 4 1.6661865873220156 3.8365319306487677 5 0.9443238006935031 2.717979218083246 6 0.5816612006162989 2.008984275198087 7 0.3975581918738707 1.6019683754425493 8 0.27042425397433134 1.245348261819711 9 0.20984087735729787 1.0871458053018936 >10 1.1724862393122202 12.461937620150444 >50 0.08327313053652836 3.2859095685578112 >100 0.036748412850255895 3.5354455888720953 >500 0.0013256564723860122 0.5203308583103201 >1k 2.8406924408271684E-4 0.3282455927870048 >5k 1.893794960551446E-4 0.764252981811125 >10k+ 9.46897480275723E-5 0.6058534978126136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11115 0.6060797735121055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8582 0.4679601094269806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5439 0.2965783075242773 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2428 0.13239421413291877 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.12001633661719696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.4528094782915475E-5 0.0 10-11 0.0 0.0 0.0 5.4528094782915475E-5 0.0 12-13 0.0 0.0 0.0 5.4528094782915475E-5 0.0 14-15 0.0 0.0 0.0 5.4528094782915475E-5 0.0 16-17 0.0 0.0 0.0 5.4528094782915475E-5 0.0 18-19 0.0 0.0 0.0 5.4528094782915475E-5 0.0 20-21 0.0 0.0 0.0 5.4528094782915475E-5 0.0 22-23 0.0 2.7264047391457737E-5 0.0 1.0905618956583095E-4 0.0 24-25 0.0 5.4528094782915475E-5 0.0 1.6358428434874644E-4 0.0 26-27 0.0 5.4528094782915475E-5 0.0 4.634888056547815E-4 0.0 28-29 0.0 5.4528094782915475E-5 0.0 0.0011996180852241404 0.0 30-31 0.0 5.4528094782915475E-5 0.0 0.0034625340187151325 0.0 32-33 0.0 5.4528094782915475E-5 0.0 0.006952332084821723 0.0 34-35 0.0 5.4528094782915475E-5 0.0 0.010060433487447906 0.0 36-37 0.0 5.4528094782915475E-5 0.0 0.014150040596166567 2.7264047391457737E-5 38-39 0.0 5.4528094782915475E-5 0.0 0.017530782472707326 5.4528094782915475E-5 40-41 0.0 5.4528094782915475E-5 0.0 0.02279274361925867 5.4528094782915475E-5 42-43 0.0 5.4528094782915475E-5 0.0 0.031353654500176395 5.4528094782915475E-5 44-45 0.0 5.4528094782915475E-5 0.0 0.04544916700156005 5.4528094782915475E-5 46-47 0.0 5.4528094782915475E-5 0.0 0.07358566390954444 5.4528094782915475E-5 48-49 0.0 5.4528094782915475E-5 0.0 0.1342481693555379 5.4528094782915475E-5 50-51 0.0 5.4528094782915475E-5 0.0 0.17945195993057483 5.4528094782915475E-5 52-53 0.0 5.4528094782915475E-5 0.0 0.2205661433968931 8.17921421743732E-5 54-55 0.0 5.4528094782915475E-5 0.0 0.2764574405493815 1.0905618956583095E-4 56-57 0.0 5.4528094782915475E-5 0.0 0.31648106212004146 1.0905618956583095E-4 58-59 0.0 5.4528094782915475E-5 0.0 0.3712817973768715 1.0905618956583095E-4 60-61 0.0 5.4528094782915475E-5 0.0 0.45749071522866086 1.0905618956583095E-4 62-63 0.0 5.4528094782915475E-5 0.0 0.517117186873779 1.0905618956583095E-4 64-65 0.0 5.4528094782915475E-5 0.0 0.5919842610107219 1.0905618956583095E-4 66-67 0.0 5.4528094782915475E-5 0.0 0.7276501608306156 1.0905618956583095E-4 68-69 0.0 5.4528094782915475E-5 0.0 0.8650882237309541 1.0905618956583095E-4 70-71 0.0 5.4528094782915475E-5 0.0 0.995410370262122 1.0905618956583095E-4 72-73 0.0 5.4528094782915475E-5 0.0 1.1560228734451996 1.0905618956583095E-4 74-75 0.0 5.4528094782915475E-5 0.0 1.3021581674634128 1.0905618956583095E-4 76-77 0.0 5.4528094782915475E-5 0.0 1.4203205488579909 1.363202369572887E-4 78-79 0.0 5.4528094782915475E-5 0.0 1.5826234229793386 1.6358428434874644E-4 80-81 0.0 5.4528094782915475E-5 0.0 1.8209657252754623 1.6358428434874644E-4 82-83 0.0 5.4528094782915475E-5 0.0 2.0321803004170853 1.6358428434874644E-4 84-85 0.0 5.4528094782915475E-5 0.0 2.276547957186721 1.6358428434874644E-4 86-87 0.0 5.4528094782915475E-5 0.0 2.7111641366539487 1.6358428434874644E-4 88-89 0.0 5.4528094782915475E-5 0.0 3.3128543985360297 1.6358428434874644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5930 0.0 46.417183 1 GTATCAA 10500 0.0 39.525723 1 TATCAAC 12905 0.0 31.99916 2 ATCAACG 12885 0.0 31.97507 3 TCAACGC 13035 0.0 31.574114 4 CAACGCA 13225 0.0 31.08456 5 AACGCAG 13680 0.0 30.139254 6 ACGCAGA 15510 0.0 26.462835 7 CGCAGAG 15545 0.0 26.219183 8 TACCTGG 1625 0.0 26.026363 2 GTACATG 10255 0.0 25.682009 1 TACATGG 10375 0.0 24.779127 2 GCAGAGT 16390 0.0 24.229143 9 GAGTACT 8710 0.0 23.804173 12-13 GTACCTG 1845 0.0 23.705349 1 ACATGGG 10500 0.0 23.30745 3 GTACTTT 9215 0.0 22.344105 14-15 CATGGGA 6235 0.0 21.79987 4 AGTACTT 8970 0.0 20.545952 12-13 CAGAGTA 15955 0.0 20.512907 10-11 >>END_MODULE