FastQCFastQC Report
Thu 26 May 2016
SRR937397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833917
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113940.6212931119565389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95420.5203070804185795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.3403643676349584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.1343026974503208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.040.377741
GTATCAA114750.034.7538761
CCGCGTG856.409301E-427.940699
TATCAAC145600.027.3418462
ATCAACG146150.027.141443
TCAACGC147100.026.9661544
CAACGCA150300.026.4552385
AACGCAG153250.026.0347236
GTACATG102900.024.0204941
TACATGG103800.023.3409232
ACGCAGA174850.022.7919967
CGCAGAG175750.022.6482548
ACATGGG103900.022.4497343
GAGTACT99400.022.22043412-13
AGTACTT103000.021.42074212-13
GCAGAGT185800.021.1419859
GTACTTT104400.020.85993814-15
TATCACG2108.6036016E-720.3595622
GTGTAGG6800.020.271371
AGAGTAC172000.019.41390210-11
Produced by FastQC (version 0.11.5)