##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937397_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1833917 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17422380620279 33.0 31.0 34.0 31.0 34.0 2 32.38787087965268 34.0 31.0 34.0 31.0 34.0 3 32.46419167279653 34.0 31.0 34.0 31.0 34.0 4 36.07804878846753 37.0 35.0 37.0 35.0 37.0 5 35.97710474356255 37.0 35.0 37.0 35.0 37.0 6 36.06428044453484 37.0 35.0 37.0 35.0 37.0 7 36.015217155411065 37.0 35.0 37.0 35.0 37.0 8 35.997874494865364 37.0 35.0 37.0 35.0 37.0 9 37.69261749577544 39.0 37.0 39.0 35.0 39.0 10-11 37.62205296095734 39.0 37.0 39.0 35.0 39.0 12-13 37.54340136440199 39.0 37.0 39.0 35.0 39.0 14-15 38.75027877488458 40.0 38.0 41.0 35.0 41.0 16-17 38.72897819257905 40.0 38.0 41.0 35.0 41.0 18-19 38.75373994570092 40.0 38.0 41.0 35.0 41.0 20-21 38.65760827780102 40.0 38.0 41.0 34.0 41.0 22-23 38.5366660541344 40.0 38.0 41.0 34.0 41.0 24-25 38.40143038098235 40.0 38.0 41.0 34.0 41.0 26-27 38.34535723263376 40.0 38.0 41.0 34.0 41.0 28-29 38.246028582536724 40.0 38.0 41.0 34.0 41.0 30-31 38.29907842067007 40.0 38.0 41.0 34.0 41.0 32-33 38.208840967175725 40.0 38.0 41.0 34.0 41.0 34-35 38.0955872048735 40.0 37.0 41.0 34.0 41.0 36-37 37.94036534914066 40.0 37.0 41.0 33.0 41.0 38-39 37.814100092861345 40.0 37.0 41.0 33.0 41.0 40-41 37.63477654659398 40.0 37.0 41.0 33.0 41.0 42-43 37.408288652103664 40.0 37.0 41.0 33.0 41.0 44-45 37.0874431612772 40.0 36.5 41.0 31.5 41.0 46-47 36.679387071497786 40.0 36.0 41.0 31.0 41.0 48-49 36.324317294621295 39.5 36.0 41.0 30.0 41.0 50-51 36.08428925627496 39.0 36.0 41.0 29.5 41.0 52-53 35.84708277419316 39.0 35.0 41.0 29.0 41.0 54-55 35.62062923240256 39.0 35.0 40.0 28.0 41.0 56-57 35.38009680917948 39.0 35.0 40.0 28.0 41.0 58-59 35.13806813503555 38.0 35.0 40.0 27.0 41.0 60-61 34.81606010522832 38.0 34.0 40.0 26.5 41.0 62-63 34.4503287226194 37.5 34.0 40.0 26.0 41.0 64-65 34.094758377832804 37.0 34.0 40.0 26.0 41.0 66-67 33.67450189948618 36.5 33.0 39.0 25.0 41.0 68-69 33.28271044981861 36.0 33.0 39.0 23.5 40.0 70-71 32.8815107226772 35.5 32.5 38.5 23.0 40.0 72-73 32.44090708576233 35.0 32.0 38.0 22.0 40.0 74-75 31.997355932683973 35.0 32.0 37.0 20.5 39.0 76-77 30.665259932701424 34.0 30.0 36.0 20.0 38.5 78-79 31.286789696589324 34.5 31.0 36.0 20.0 39.0 80-81 31.14562791009626 35.0 31.0 36.0 19.5 37.5 82-83 30.820036021259412 34.0 31.0 35.5 18.0 37.0 84-85 30.464580458112337 34.0 31.0 35.0 16.0 37.0 86-87 30.09119987436727 34.0 31.0 35.0 9.0 36.0 88-89 29.708975378929363 34.0 30.0 35.0 6.5 36.0 90-91 29.27593969628942 34.0 29.5 35.0 2.0 35.5 92-93 28.917788536776747 34.0 29.0 35.0 2.0 35.0 94-95 28.524491839052693 34.0 29.0 35.0 2.0 35.0 96-97 28.05867086678405 34.0 29.0 35.0 2.0 35.0 98-99 27.5853705483945 33.5 28.5 35.0 2.0 35.0 100-101 25.715041902114436 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15234925615479966 1101 2 0.12818808169068063 1101 3 0.169061732592084 1101 4 0.11715993335412378 1101 5 -0.04897725338057768 1101 6 0.033110378180019495 1101 7 0.03684723841750781 1101 8 0.050173249034074274 1101 9 0.11740086349861656 1101 10-11 0.08577980753561576 1101 12-13 0.16272186495692864 1101 14-15 0.07985465315611151 1101 16-17 0.07287247857446033 1101 18-19 0.07805569851173288 1101 20-21 0.14715534691569587 1101 22-23 0.1524144386603581 1101 24-25 0.15351169949808252 1101 26-27 0.008395157839402145 1101 28-29 0.08638586302071616 1101 30-31 0.16002252826736196 1101 32-33 0.1705754967564843 1101 34-35 0.07761326784016376 1101 36-37 0.11253837455358706 1101 38-39 -0.013757739467997965 1101 40-41 0.07609691281118813 1101 42-43 0.1062729053660263 1101 44-45 0.11271138249230717 1101 46-47 0.11359058103001729 1101 48-49 0.2289895967887574 1101 50-51 0.27114190154673423 1101 52-53 0.17682022819612797 1101 54-55 0.13478266544645834 1101 56-57 0.08054011368974301 1101 58-59 0.0544757181791411 1101 60-61 -0.04680134893619936 1101 62-63 0.03411908343859693 1101 64-65 0.03499743861096505 1101 66-67 -0.018140376423332327 1101 68-69 0.042458870152849215 1101 70-71 -0.1349592089051086 1101 72-73 -0.17747253367162585 1101 74-75 -0.12535046449336917 1101 76-77 -0.11675737868317526 1101 78-79 -0.21807216953795105 1101 80-81 -0.20123307659191525 1101 82-83 -0.22783481388306726 1101 84-85 -0.14151901136591505 1101 86-87 -0.16515668477827106 1101 88-89 -0.1662709027186402 1101 90-91 0.008340578703617041 1101 92-93 -0.10425631750120345 1101 94-95 -0.09140568940836147 1101 96-97 -0.01096750144791514 1101 98-99 -0.23383327158887823 1101 100-101 -0.06426469542561009 1102 1 0.08691093059099586 1102 2 0.08376963060187137 1102 3 0.11384808606771912 1102 4 0.06003197174750596 1102 5 0.06946783598260708 1102 6 0.016350296177542134 1102 7 0.032193685211012735 1102 8 0.05868239191529767 1102 9 0.12281554822992291 1102 10-11 0.11661389301396241 1102 12-13 0.06600709012010242 1102 14-15 0.10977439355500707 1102 16-17 0.06459638357218722 1102 18-19 0.048924713642854556 1102 20-21 0.06296184066432176 1102 22-23 0.0945488876364422 1102 24-25 0.148974527839286 1102 26-27 0.12549360068086912 1102 28-29 -0.07869965368487897 1102 30-31 0.027139671895866968 1102 32-33 0.10414508020167546 1102 34-35 0.1513746776934468 1102 36-37 0.18266898388788633 1102 38-39 0.04287109148790336 1102 40-41 0.2715874410998751 1102 42-43 0.2632291804578273 1102 44-45 0.26453471872029866 1102 46-47 0.24053551034484144 1102 48-49 0.2120169713235569 1102 50-51 0.3320197031999186 1102 52-53 0.29400515031161234 1102 54-55 0.23130900849521652 1102 56-57 0.2976459988748772 1102 58-59 0.23922107801075754 1102 60-61 0.1430211321817012 1102 62-63 0.10117023322374052 1102 64-65 0.1997676999169684 1102 66-67 0.33132322425088745 1102 68-69 0.3219416576447074 1102 70-71 0.1397763315894025 1102 72-73 0.2489621774192443 1102 74-75 0.3253932762487928 1102 76-77 0.26350148197754564 1102 78-79 0.2230560441970475 1102 80-81 0.1999722793134886 1102 82-83 0.2922422371566 1102 84-85 0.2653465126984855 1102 86-87 0.22518227980186722 1102 88-89 0.22414732661869508 1102 90-91 0.22462486332162257 1102 92-93 0.503294471747953 1102 94-95 0.5752100642865194 1102 96-97 0.651480919503193 1102 98-99 0.5330774609161999 1102 100-101 1.1411004179809048 1103 1 0.08519605060222091 1103 2 0.06128120667867876 1103 3 0.11644127620501621 1103 4 0.13080766148650014 1103 5 -0.014496811348045924 1103 6 0.0585556267480456 1103 7 0.05569876978805155 1103 8 0.020848623770760355 1103 9 0.038610845135792715 1103 10-11 4.5610210704438714E-4 1103 12-13 0.02835429462664507 1103 14-15 0.05235276098377284 1103 16-17 0.008179849785371118 1103 18-19 0.032504213546928895 1103 20-21 -0.004882817193504252 1103 22-23 -0.007637559853755249 1103 24-25 0.10705383481690944 1103 26-27 0.05631011753641246 1103 28-29 -0.060229077368802564 1103 30-31 -0.005463232171386778 1103 32-33 0.055538099301685406 1103 34-35 0.04906545882706581 1103 36-37 0.11352802490724656 1103 38-39 0.06403528943416603 1103 40-41 0.15546041652346076 1103 42-43 0.15868626720771317 1103 44-45 0.14102142325634048 1103 46-47 0.1672565778933972 1103 48-49 0.18913965706352087 1103 50-51 0.12612152329291604 1103 52-53 0.14658602411851263 1103 54-55 0.13531061069597428 1103 56-57 -0.026836102861338418 1103 58-59 0.15602411921353365 1103 60-61 0.07815296982719389 1103 62-63 -0.021228321660160532 1103 64-65 -0.050040794372939956 1103 66-67 0.14523454392853807 1103 68-69 0.24041754458215792 1103 70-71 0.13381893139528245 1103 72-73 0.17809252299568357 1103 74-75 0.18770846025594423 1103 76-77 0.22866625527378304 1103 78-79 0.23275694255429968 1103 80-81 0.2817526151603218 1103 82-83 0.13085519691482617 1103 84-85 0.13448937252884718 1103 86-87 0.23219367177060235 1103 88-89 0.16068471525487027 1103 90-91 0.15546097656601887 1103 92-93 0.2717411894605988 1103 94-95 0.49807750196656997 1103 96-97 0.2864980790810918 1103 98-99 0.4100087095334324 1103 100-101 0.4398261040063822 1104 1 0.156834410563782 1104 2 0.11709045321939726 1104 3 0.11795642028406661 1104 4 0.01392150047642815 1104 5 0.012052761131222667 1104 6 0.0173512854813751 1104 7 6.272676693441781E-4 1104 8 0.08058393489258009 1104 9 0.06856703787986618 1104 10-11 0.043300933331806846 1104 12-13 0.0655112498505872 1104 14-15 0.10124786208989889 1104 16-17 0.09114507980045516 1104 18-19 -0.004014429812144726 1104 20-21 -0.1422551278274895 1104 22-23 -0.007690662532347403 1104 24-25 -0.0382755343016683 1104 26-27 -0.04252623689109214 1104 28-29 0.03516139418494646 1104 30-31 0.039030628628921704 1104 32-33 0.13049601469272432 1104 34-35 0.11855860799258267 1104 36-37 -0.050125596430412145 1104 38-39 -0.022030606965550703 1104 40-41 0.042633518495328815 1104 42-43 0.09286907980433767 1104 44-45 0.10718520134014398 1104 46-47 0.05521434310495721 1104 48-49 0.03779947698556185 1104 50-51 -0.10881032172579097 1104 52-53 -0.0760176561621293 1104 54-55 -0.07017692784273777 1104 56-57 0.07824732527483746 1104 58-59 -0.03995273940474675 1104 60-61 -0.05535874662610496 1104 62-63 -0.059611742630906406 1104 64-65 -0.2543690062261206 1104 66-67 -0.17297653382026112 1104 68-69 -0.09333867976831556 1104 70-71 -0.137371344041739 1104 72-73 0.053621709346828084 1104 74-75 -0.09372415381911381 1104 76-77 -0.07591578934621168 1104 78-79 -0.10638817302990944 1104 80-81 -0.16831810661403424 1104 82-83 -0.060213246646643626 1104 84-85 -0.10160984801020945 1104 86-87 -0.15632407640098833 1104 88-89 -0.024850743298049593 1104 90-91 -0.038499158143995516 1104 92-93 -0.14526727457632305 1104 94-95 -0.18594683692471037 1104 96-97 -0.013673105893893478 1104 98-99 -0.03135523423858544 1104 100-101 0.34539364011080664 1105 1 -0.018196167841374233 1105 2 -0.014151423560797127 1105 3 -0.032212372512404386 1105 4 0.0025712219204550024 1105 5 -0.069979474357595 1105 6 -0.07745113015945293 1105 7 -0.0011544695244225522 1105 8 -0.06665315995685006 1105 9 -0.12863208656732894 1105 10-11 -0.20675969168015484 1105 12-13 -0.10472357102909058 1105 14-15 -0.06089415785040586 1105 16-17 2.1989957399881632E-4 1105 18-19 -0.06085303752151816 1105 20-21 -0.07623044812963542 1105 22-23 -0.10062869951573106 1105 24-25 -0.11139323403980939 1105 26-27 0.010199548734618702 1105 28-29 -0.06009911255506495 1105 30-31 -0.03646399701695202 1105 32-33 -0.04754230882360844 1105 34-35 -0.10742091138933318 1105 36-37 -0.19575676662078223 1105 38-39 -0.2817669838872092 1105 40-41 -0.09012951428685056 1105 42-43 -0.2542082628205762 1105 44-45 -0.23238485032938172 1105 46-47 -0.34478363606130813 1105 48-49 -0.29423095038885805 1105 50-51 -0.3216130479368502 1105 52-53 -0.24774342689747897 1105 54-55 -0.33024408333844946 1105 56-57 -0.4160900858882499 1105 58-59 -0.42881964729514976 1105 60-61 -0.4159853539866205 1105 62-63 -0.3426105748017392 1105 64-65 -0.47247684642284327 1105 66-67 -0.5876963930851389 1105 68-69 -0.4148868298545736 1105 70-71 -0.2659617806056431 1105 72-73 -0.26519725160428465 1105 74-75 -0.33033591762406544 1105 76-77 -0.3485120076217818 1105 78-79 -0.24898926257837317 1105 80-81 -0.19948414894943767 1105 82-83 -0.17065138467171437 1105 84-85 -0.18810855148583272 1105 86-87 -0.04776116571970945 1105 88-89 -0.009807231557005025 1105 90-91 -0.003536713743510944 1105 92-93 -0.2298446969709893 1105 94-95 -0.22762812804602106 1105 96-97 -0.206323759048626 1105 98-99 -0.031866079734147235 1105 100-101 0.34315795984499076 1106 1 -0.20402839615280755 1106 2 -0.004003767907256872 1106 3 -0.028254384981380554 1106 4 -0.04806041787022508 1106 5 -0.10249184596641214 1106 6 -0.15225106710365566 1106 7 -0.12467119012852379 1106 8 -0.11624468822531497 1106 9 -0.17858989482207477 1106 10-11 -0.3420700096482747 1106 12-13 -0.3338309297022022 1106 14-15 -0.2744778906916565 1106 16-17 -0.17633941769248196 1106 18-19 -0.2379022735881975 1106 20-21 -0.06389593095465784 1106 22-23 -0.2271173881097468 1106 24-25 -0.17966272338060207 1106 26-27 -0.07876630531594486 1106 28-29 -0.16606066706587086 1106 30-31 -0.08099097036969027 1106 32-33 -0.23143340775629895 1106 34-35 -0.40269358133861743 1106 36-37 -0.2393124025415645 1106 38-39 -0.13380146057047426 1106 40-41 -0.3265941551315521 1106 42-43 -0.6723261400657776 1106 44-45 -0.5310583604044012 1106 46-47 -0.29259260172990054 1106 48-49 -0.25248121389408595 1106 50-51 -0.29959374791799576 1106 52-53 -0.2742222243863779 1106 54-55 -0.3355991894669117 1106 56-57 -0.37013431149199505 1106 58-59 -0.39809849300318234 1106 60-61 -0.31373682760673205 1106 62-63 -0.2484032077830065 1106 64-65 -0.4342098607680427 1106 66-67 -0.536586425633061 1106 68-69 -0.3604641149316734 1106 70-71 -0.4985232986429935 1106 72-73 -0.3052508181204203 1106 74-75 -0.4349706731128542 1106 76-77 -0.29127882790967163 1106 78-79 -0.2662098311314587 1106 80-81 -0.33113707625290445 1106 82-83 -0.3523386217460498 1106 84-85 -0.419150084854401 1106 86-87 -0.3206374372676386 1106 88-89 -0.4298121461998612 1106 90-91 -0.642190688885929 1106 92-93 -0.5994351124901343 1106 94-95 -0.7821134351184682 1106 96-97 -0.5486216791346763 1106 98-99 -0.4108684999639287 1106 100-101 -0.0941177516191054 1107 1 0.0834021188759877 1107 2 0.14551197415981676 1107 3 0.16581721327611376 1107 4 0.09011077895129915 1107 5 0.030320976405533884 1107 6 0.106943349340483 1107 7 0.05895482009306363 1107 8 0.055299926578889824 1107 9 0.12003484801721243 1107 10-11 0.08584099064057682 1107 12-13 0.11592767818150662 1107 14-15 0.022870627650014796 1107 16-17 0.0052912022637841005 1107 18-19 0.006617034833702462 1107 20-21 0.08577897074953 1107 22-23 0.20509386978191912 1107 24-25 0.13810833432361136 1107 26-27 0.13534024911370324 1107 28-29 0.05761904479624036 1107 30-31 0.03374396988650119 1107 32-33 0.10551491252005007 1107 34-35 0.07979149007690722 1107 36-37 8.919112740883861E-4 1107 38-39 0.12757108498160363 1107 40-41 0.13225276277155018 1107 42-43 0.07744101527991631 1107 44-45 0.24430107510941923 1107 46-47 -0.1375313252696131 1107 48-49 -0.14244590403384905 1107 50-51 -0.037040768390646406 1107 52-53 -0.13102865679363873 1107 54-55 -0.03602980470733286 1107 56-57 -0.040713652571092496 1107 58-59 0.0460482639718407 1107 60-61 0.18940510152597057 1107 62-63 0.11919807964161322 1107 64-65 0.2094493961333015 1107 66-67 0.3464901891004075 1107 68-69 0.24388713270303697 1107 70-71 0.2766445558261452 1107 72-73 0.19330200814209775 1107 74-75 0.22414622669320394 1107 76-77 0.0889343870560495 1107 78-79 0.14054767442439342 1107 80-81 0.2104802214522472 1107 82-83 0.2268108763116139 1107 84-85 0.24153777951800848 1107 86-87 0.13789852986294804 1107 88-89 0.13043125014889867 1107 90-91 0.14319138266517228 1107 92-93 0.07134023073467333 1107 94-95 0.0952555381839737 1107 96-97 0.3431910312360458 1107 98-99 0.39431863050570826 1107 100-101 0.6947201472378914 1108 1 -0.11076720413554852 1108 2 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-0.24321801454427217 2316 96-97 -0.02514588362133452 2316 98-99 -0.018121579296352053 2316 100-101 -0.3804619877640256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 16.0 5 50.0 6 92.0 7 177.0 8 466.0 9 907.0 10 1514.0 11 2441.0 12 3888.0 13 5578.0 14 7228.0 15 10418.0 16 14547.0 17 21484.0 18 19557.0 19 10722.0 20 8593.0 21 8600.0 22 8889.0 23 9201.0 24 10794.0 25 12834.0 26 15573.0 27 18932.0 28 24012.0 29 30275.0 30 37511.0 31 47870.0 32 61856.0 33 82435.0 34 113742.0 35 164848.0 36 258158.0 37 414787.0 38 367173.0 39 38734.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2377578005097 20.02104905936675 15.34578999716836 21.395403142955193 2 15.618045963912216 21.24807175024824 39.789641515946464 23.344240769893077 3 19.312487969739088 25.87843397492907 30.258566772651108 24.55051128268073 4 12.852490052712309 17.574350420438876 37.18265330437528 32.39050622247353 5 13.128983983350711 38.456046928651524 33.77697982975493 14.637989258242836 6 30.849596792003126 37.19677608092405 17.516932336632465 14.436694790440352 7 27.896137066181293 33.98883373675036 20.48522370423525 17.6298054928331 8 26.918830023387102 35.04656971934935 19.355019883669762 18.679580373593787 9 26.962997780161263 16.682216261695597 20.205658162283243 36.1491277958599 10-11 24.755782295491016 27.741468125329554 26.675034911612684 20.827714667566745 12-13 25.90144822270517 25.038436507306823 27.88487833233408 21.175236937653928 14-15 22.904199044994947 26.004066705308908 26.700445003781525 24.391289245914617 16-17 21.405957848692168 29.595123443427372 27.24458086162024 21.75433784626022 18-19 21.367597334012387 27.41977963015774 30.57602934047724 20.636593695352627 20-21 22.324510869357773 26.41368175331817 30.79097909011149 20.470828287212562 22-23 21.937317432127145 26.447006309480255 30.64334778153963 20.97232847685297 24-25 21.587450890581078 26.61322907550241 30.904294910069442 20.89502512384707 26-27 21.45443352238368 26.873040722437334 30.735431481381514 20.93709427379747 28-29 21.25260105394488 27.00401118507726 30.903192807349694 20.84019495362817 30-31 21.38550981314858 26.786435809254183 30.852050556268356 20.97600382132888 32-33 21.34368676445008 26.893474459313044 30.60160301693043 21.161235759306447 34-35 21.631158880145612 26.8767070701673 30.619542760114005 20.872591289573084 36-37 21.673281833365415 26.818280216607405 30.49759067613202 21.010847273895163 38-39 21.54189638898598 26.892002200753907 30.614253534920067 20.95184787534005 40-41 21.633094627510406 26.841291072605795 30.540177118157473 20.985437181726326 42-43 21.445600532933252 27.0285835357329 30.295090246072604 21.23072568526125 44-45 21.634348773690412 27.17693876004203 29.979273871173014 21.209438595094543 46-47 21.80507623845572 27.41743492208208 29.351055691179045 21.426433148283156 48-49 21.858922732053852 27.545357832442797 29.10409794990722 21.49162148559613 50-51 22.107898012832642 27.74364924912087 28.862320377639772 21.286132360406715 52-53 22.129681986698415 27.948129604556804 28.56773234557507 21.354456063169707 54-55 21.827814453980196 27.997532058430124 28.610127939268786 21.56452554832089 56-57 21.84381844979898 28.3391233081977 28.447852329194834 21.369205912808486 58-59 21.878225677607002 27.876561480154226 28.61127302925923 21.63393981297954 60-61 21.811728666019235 27.976157045275222 28.870854024473296 21.341260264232243 62-63 21.82732370112715 27.955818065921196 28.985117647090895 21.231740585860756 64-65 21.689585733705506 27.808401361675582 28.941849603880655 21.560163300738257 66-67 21.61957166000424 28.079078824177977 28.676515894666988 21.624833621150792 68-69 21.73383528262184 28.087476150774542 28.62250581678451 21.556182749819104 70-71 21.703190493353844 28.0783699589458 28.53046239279095 21.68797715490941 72-73 21.708921862036796 28.046478670103024 28.355216826938413 21.889382640921763 74-75 21.65092531450442 28.339259628434654 28.13826907106483 21.871545985996093 76-77 21.781547365556893 28.161334455158006 28.26054832361552 21.79656985566959 78-79 21.794906748778704 28.15195562285534 28.126572794733896 21.926564833632057 80-81 21.730863501456174 28.450878638455286 28.02037933014417 21.797878529944377 82-83 21.711571141791737 28.489270065649297 28.024471191766885 21.774687600792078 84-85 21.727387758429415 28.20135662407325 27.970290483537596 22.100965133959743 86-87 21.63129520038257 28.135160969662206 28.046879984208662 22.186663845746562 88-89 21.770559954458136 27.998513564136218 28.077415717287096 22.15351076411855 90-91 21.770299180839686 28.001774547405883 28.031359426560513 22.196566845193917 92-93 21.51161696949736 27.872002172430687 28.196476289069604 22.419904569002348 94-95 21.36928770495066 27.842263308535774 28.270118004249923 22.518330982263645 96-97 21.336516319986128 28.00603844121626 28.158144561613202 22.499300677184408 98-99 21.19308757117291 27.962270858938858 27.96352500717046 22.88111656271777 100-101 21.086422365427673 28.289863150113316 27.958229794483845 22.66548468997517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 709.0 1 503.5 2 369.5 3 484.0 4 471.0 5 453.5 6 771.5 7 1541.0 8 2467.5 9 3110.0 10 3116.0 11 2955.0 12 3138.5 13 3419.0 14 3593.0 15 3803.0 16 4018.5 17 4186.0 18 4382.5 19 4632.5 20 4991.0 21 5483.0 22 6190.0 23 7040.5 24 8250.0 25 10053.5 26 12371.5 27 15456.5 28 18304.5 29 21829.0 30 26086.0 31 28629.0 32 31383.5 33 34998.0 34 39416.0 35 43394.5 36 47959.0 37 52302.0 38 54423.5 39 56067.0 40 57358.0 41 58953.5 42 60258.5 43 62978.5 44 66097.5 45 66619.0 46 65965.5 47 66705.0 48 70497.5 49 70372.0 50 66330.0 51 62911.0 52 59493.0 53 57266.0 54 55497.0 55 51252.5 56 44528.0 57 38993.0 58 35677.5 59 31905.5 60 26464.0 61 21445.0 62 16952.5 63 12872.5 64 9326.0 65 6857.5 66 5004.5 67 3722.5 68 2765.0 69 1829.5 70 1250.0 71 871.0 72 702.5 73 590.5 74 439.0 75 311.0 76 199.5 77 99.0 78 56.0 79 40.0 80 23.0 81 12.0 82 11.5 83 7.5 84 3.0 85 2.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05758166809075874 2 0.0 3 0.0 4 0.0 5 0.017721630804447528 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002644612596971401 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.452809478291548E-4 24-25 1.0905618956583095E-4 26-27 7.633933269608166E-4 28-29 0.002453764265231196 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01589493962921986 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.7264047391457737E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.999045213060351E-4 84-85 0.00144499451174726 86-87 0.0 88-89 0.0 90-91 0.01145089990441225 92-93 0.00144499451174726 94-95 0.0 96-97 0.0 98-99 1.635842843487464E-4 100-101 0.01447720916486406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1833917.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.560953328115424 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14505327750675 42.86252704646531 2 12.883071696365192 14.315914904890226 3 4.154766077220889 6.925282923171211 4 1.8345821780484075 4.077245390845596 5 1.000147711051601 2.7784580147479745 6 0.6081036879118609 2.027209237363546 7 0.4306993651735075 1.6751047128797936 8 0.3064400293260277 1.36208801337998 9 0.23856690078853537 1.1929503996310462 >10 1.262006917458115 12.937257507967075 >50 0.0943970322361777 3.576376065938743 >100 0.03990495558889799 3.83824597231891 >500 0.001474024776543812 0.5313010643149262 >1k 4.913415921812707E-4 0.41615216444439085 >5k 1.9653663687250824E-4 0.8617876887418349 >10k+ 9.826831843625412E-5 0.6220988928994213 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11394 0.6212931119565389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9542 0.5203070804185795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6242 0.3403643676349584 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.1343026974503208 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.4528094782915475E-5 7 0.0 0.0 0.0 0.0 5.4528094782915475E-5 8 0.0 0.0 0.0 0.0 5.4528094782915475E-5 9 0.0 0.0 0.0 0.0 5.4528094782915475E-5 10-11 0.0 0.0 0.0 0.0 5.4528094782915475E-5 12-13 0.0 0.0 0.0 0.0 5.4528094782915475E-5 14-15 0.0 0.0 0.0 0.0 5.4528094782915475E-5 16-17 0.0 0.0 0.0 0.0 5.4528094782915475E-5 18-19 0.0 0.0 0.0 0.0 5.4528094782915475E-5 20-21 0.0 0.0 0.0 0.0 5.4528094782915475E-5 22-23 0.0 0.0 0.0 5.4528094782915475E-5 5.4528094782915475E-5 24-25 0.0 0.0 0.0 1.0905618956583095E-4 5.4528094782915475E-5 26-27 0.0 0.0 0.0 4.08960710871866E-4 5.4528094782915475E-5 28-29 0.0 0.0 0.0 0.0011450899904412249 5.4528094782915475E-5 30-31 0.0 0.0 0.0 0.003298949734366386 5.4528094782915475E-5 32-33 0.0 0.0 0.0 0.006925068037430265 5.4528094782915475E-5 34-35 0.0 0.0 0.0 0.010033169440056448 1.0905618956583095E-4 36-37 0.0 0.0 0.0 0.014231832738340939 1.0905618956583095E-4 38-39 0.0 0.0 0.0 0.017585310567490242 1.0905618956583095E-4 40-41 0.0 0.0 0.0 0.023092648140564706 1.0905618956583095E-4 42-43 0.0 0.0 0.0 0.031735351163656805 1.0905618956583095E-4 44-45 0.0 0.0 0.0 0.04588539175982337 1.0905618956583095E-4 46-47 0.0 0.0 0.0 0.07429452914172233 1.0905618956583095E-4 48-49 0.0 0.0 0.0 0.13615665267293992 1.0905618956583095E-4 50-51 0.0 0.0 0.0 0.1817966680062402 1.0905618956583095E-4 52-53 0.0 0.0 0.0 0.2230199076621243 1.0905618956583095E-4 54-55 0.0 0.0 0.0 0.27940195766765885 1.0905618956583095E-4 56-57 0.0 0.0 0.0 0.3197800118544078 1.0905618956583095E-4 58-59 0.0 0.0 0.0 0.37531687639080724 1.0905618956583095E-4 60-61 0.0 0.0 0.0 0.4627526763752122 1.0905618956583095E-4 62-63 0.0 0.0 0.0 0.5226245244468534 1.0905618956583095E-4 64-65 0.0 0.0 0.0 0.5977097109629279 1.0905618956583095E-4 66-67 0.0 0.0 0.0 0.7343025883941312 1.0905618956583095E-4 68-69 0.0 0.0 0.0 0.8732129098536083 1.0905618956583095E-4 70-71 0.0 0.0 0.0 1.0051436351808725 1.0905618956583095E-4 72-73 0.0 0.0 0.0 1.1675555654917862 1.0905618956583095E-4 74-75 0.0 0.0 0.0 1.3139907640313058 1.0905618956583095E-4 76-77 0.0 0.0 0.0 1.4334072916058904 1.0905618956583095E-4 78-79 0.0 0.0 0.0 1.5976186490446405 1.0905618956583095E-4 80-81 0.0 0.0 0.0 1.8380057548951234 1.0905618956583095E-4 82-83 0.0 0.0 0.0 2.051265133591106 1.0905618956583095E-4 84-85 0.0 0.0 0.0 2.297704857962492 1.0905618956583095E-4 86-87 0.0 0.0 0.0 2.7382373357136665 1.0905618956583095E-4 88-89 0.0 0.0 0.0 3.350206143462327 1.0905618956583095E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7075 0.0 40.37774 1 GTATCAA 11475 0.0 34.753876 1 CCGCGTG 85 6.409301E-4 27.94069 9 TATCAAC 14560 0.0 27.341846 2 ATCAACG 14615 0.0 27.14144 3 TCAACGC 14710 0.0 26.966154 4 CAACGCA 15030 0.0 26.455238 5 AACGCAG 15325 0.0 26.034723 6 GTACATG 10290 0.0 24.020494 1 TACATGG 10380 0.0 23.340923 2 ACGCAGA 17485 0.0 22.791996 7 CGCAGAG 17575 0.0 22.648254 8 ACATGGG 10390 0.0 22.449734 3 GAGTACT 9940 0.0 22.220434 12-13 AGTACTT 10300 0.0 21.420742 12-13 GCAGAGT 18580 0.0 21.141985 9 GTACTTT 10440 0.0 20.859938 14-15 TATCACG 210 8.6036016E-7 20.359562 2 GTGTAGG 680 0.0 20.27137 1 AGAGTAC 17200 0.0 19.413902 10-11 >>END_MODULE