FastQCFastQC Report
Thu 26 May 2016
SRR937396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048941
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118600.5788356033677886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89900.43876324403679756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57770.2819505295662491No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28090.137095211623956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.11952515958243795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.042.426321
GTATCAA118900.038.927451
ATCGCGT500.001614861338.005988
ATCAACG142800.032.2055243
TCAACGC143750.032.0619244
TATCAAC143900.032.0576022
CAACGCA145800.031.5149025
AACGCAG150300.030.5683486
ACGCAGA171550.026.4752317
CGCAGAG172300.026.442068
GTACATG114250.025.5382461
TACATGG114250.025.2409132
GCAGAGT183350.024.4073899
CCGTTGC2151.6607373E-924.3055559
ACATGGG117500.023.694253
TACCTGG18850.023.691312
GAGTACT96950.023.17484512-13
GTACTTT102200.021.74983614-15
AGTACTT99500.021.45903212-13
GTACCTG21600.021.120121
Produced by FastQC (version 0.11.5)