FastQCFastQC Report
Thu 26 May 2016
SRR937396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048941
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123150.6010421969202627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107230.5233435223366607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.32904803017754053No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.13304433851438377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.041.154811
GTATCAA129300.035.2558251
TATCAAC160450.028.3291232
ATCAACG160250.028.3052023
TCAACGC162650.027.9167424
CAACGCA164900.027.5070255
AACGCAG168900.026.8837096
TACCTGG19500.025.3314272
GTACATG115750.024.7221531
GTACCTG21200.024.4400141
TACATGG117250.023.8596122
ACGCAGA191100.023.6646887
CGCAGAG191450.023.5221758
GAGTACT106400.022.85876812-13
ACATGGG117650.022.849963
ACCGTCG1252.255653E-422.8016248
AGTACTT109250.021.82763912-13
GCAGAGT203950.021.7544279
GTACTTT111500.021.5331314-15
GGACCGA5000.019.94856
Produced by FastQC (version 0.11.5)