##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937396_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048941 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24462978680206 33.0 31.0 34.0 31.0 34.0 2 32.44431977299493 34.0 31.0 34.0 31.0 34.0 3 32.4936950356306 34.0 31.0 34.0 31.0 34.0 4 36.12960744111226 37.0 35.0 37.0 35.0 37.0 5 36.059173006933825 37.0 35.0 37.0 35.0 37.0 6 36.102879487501106 37.0 35.0 37.0 35.0 37.0 7 36.04738057367196 37.0 35.0 37.0 35.0 37.0 8 36.0267455236632 37.0 35.0 37.0 35.0 37.0 9 37.73463852790295 39.0 37.0 39.0 35.0 39.0 10-11 37.662386569452224 39.0 37.0 39.0 35.0 39.0 12-13 37.566870641956015 39.0 37.0 39.0 35.0 39.0 14-15 38.76710920421817 40.0 38.0 41.0 35.0 41.0 16-17 38.741396653197924 40.0 38.0 41.0 35.0 41.0 18-19 38.7552699174842 40.0 38.0 41.0 35.0 41.0 20-21 38.672993756286786 40.0 38.0 41.0 34.0 41.0 22-23 38.52865651085122 40.0 38.0 41.0 34.0 41.0 24-25 38.466306496868384 40.0 38.0 41.0 34.0 41.0 26-27 38.411200468925166 40.0 38.0 41.0 34.0 41.0 28-29 38.28905541936054 40.0 38.0 41.0 34.0 41.0 30-31 38.34225460860025 40.0 38.0 41.0 34.0 41.0 32-33 38.2700785430132 40.0 38.0 41.0 34.0 41.0 34-35 38.144794798874145 40.0 37.5 41.0 34.0 41.0 36-37 37.982134673472785 40.0 37.0 41.0 33.0 41.0 38-39 37.85535454656821 40.0 37.0 41.0 33.0 41.0 40-41 37.671480779583206 40.0 37.0 41.0 33.0 41.0 42-43 37.44741673869575 40.0 37.0 41.0 33.0 41.0 44-45 37.134879432838716 40.0 36.5 41.0 32.0 41.0 46-47 36.72936189963498 40.0 36.0 41.0 31.0 41.0 48-49 36.36243771782594 39.5 36.0 41.0 30.0 41.0 50-51 36.10595302646587 39.0 36.0 41.0 29.5 41.0 52-53 35.859780491483164 39.0 35.0 41.0 29.0 41.0 54-55 35.63461100148808 39.0 35.0 40.0 28.0 41.0 56-57 35.37280819701495 39.0 35.0 40.0 28.0 41.0 58-59 35.110935112333635 38.0 35.0 40.0 27.0 41.0 60-61 34.79828970185086 38.0 34.0 40.0 26.0 41.0 62-63 34.434623056496015 37.5 34.0 40.0 26.0 41.0 64-65 34.0388059002187 37.0 34.0 40.0 25.5 41.0 66-67 33.62930655397105 36.5 33.0 39.0 24.5 41.0 68-69 33.20658964801817 36.0 33.0 39.0 23.0 40.0 70-71 32.81085692560205 35.5 32.5 38.5 22.5 40.0 72-73 32.34697070340239 35.0 32.0 38.0 21.5 40.0 74-75 31.87825393703381 35.0 31.5 37.0 20.0 39.0 76-77 30.613670671825105 34.0 30.0 36.0 19.5 38.5 78-79 31.199202173220215 34.5 31.0 36.0 20.0 39.0 80-81 31.007720817729744 34.5 31.0 36.0 18.5 37.5 82-83 30.66838332582539 34.0 31.0 35.5 17.5 37.0 84-85 30.31857554707529 34.0 31.0 35.0 13.0 37.0 86-87 29.916395835702442 34.0 30.0 35.0 7.0 36.0 88-89 29.47496682432535 34.0 30.0 35.0 4.5 36.0 90-91 28.985548876224353 34.0 29.0 35.0 2.0 35.0 92-93 28.656231194553673 34.0 29.0 35.0 2.0 35.0 94-95 28.254166176576092 34.0 29.0 35.0 2.0 35.0 96-97 27.81268274684337 33.5 28.5 35.0 2.0 35.0 98-99 27.110725491851646 33.0 27.0 35.0 2.0 35.0 100-101 24.699804435559635 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0710933333412811 1101 2 -0.04959473251844315 1101 3 -0.03922096066526137 1101 4 -0.05321131070696339 1101 5 -0.0666092825482636 1101 6 -0.1124417256450343 1101 7 -0.13072743113278307 1101 8 -0.0701651039860991 1101 9 -0.03620920126849114 1101 10-11 -0.06371571774393914 1101 12-13 -0.14656384553889978 1101 14-15 -0.2898741254366044 1101 16-17 -0.2043401274740404 1101 18-19 -0.06367178056969891 1101 20-21 -0.08364905279957213 1101 22-23 -0.05647988124294301 1101 24-25 -0.13078783923842963 1101 26-27 -0.2028406109552705 1101 28-29 -0.1760522346002631 1101 30-31 -0.14012225633071296 1101 32-33 -0.07752299520912231 1101 34-35 -0.13639930245521015 1101 36-37 -0.19195254338301027 1101 38-39 -0.24544084149736278 1101 40-41 -0.17848382296152465 1101 42-43 -0.23681529520348477 1101 44-45 -0.25494942648928287 1101 46-47 -0.3355389614566491 1101 48-49 -0.4826113399892833 1101 50-51 -0.3584512850347039 1101 52-53 -0.4454645454365078 1101 54-55 -0.37936992506464406 1101 56-57 -0.31896756997299747 1101 58-59 -0.3796188041512991 1101 60-61 -0.4295085510109473 1101 62-63 -0.5202306146539897 1101 64-65 -0.522286824481732 1101 66-67 -0.6222631990003364 1101 68-69 -0.5925903253683913 1101 70-71 -0.4310174455656153 1101 72-73 -0.3959124189129213 1101 74-75 -0.5192974986900758 1101 76-77 -0.5278251252067356 1101 78-79 -0.639569472555312 1101 80-81 -0.6970586586413496 1101 82-83 -0.8323507202276943 1101 84-85 -0.735628341774337 1101 86-87 -0.8021521263919311 1101 88-89 -0.8772033936996664 1101 90-91 -0.80264817349439 1101 92-93 -0.7495675848702277 1101 94-95 -0.7247327572780762 1101 96-97 -0.7842130086350032 1101 98-99 -0.8511704672357929 1101 100-101 -0.25162495453368194 1102 1 -0.11576913413149015 1102 2 -0.056894662340091884 1102 3 -0.09358931811772209 1102 4 -0.031643990443271264 1102 5 -0.12435616036022168 1102 6 -0.15997359519275278 1102 7 -0.13889897718078004 1102 8 -0.16833102540361722 1102 9 -0.11805505875981481 1102 10-11 -0.1697661966435149 1102 12-13 -0.18598778435765695 1102 14-15 -0.1518837189721367 1102 16-17 -0.18349733100218657 1102 18-19 -0.2265465712292425 1102 20-21 -0.14759309895262618 1102 22-23 -0.18350412933001792 1102 24-25 -0.17918772686095252 1102 26-27 -0.23786530578885134 1102 28-29 -0.32245670252576275 1102 30-31 -0.02464303723898098 1102 32-33 0.020182650664835933 1102 34-35 -0.08058808623969327 1102 36-37 -0.15305048046199232 1102 38-39 -0.11676446615431502 1102 40-41 0.003383306801630681 1102 42-43 0.03818005598473917 1102 44-45 -0.005767502220876963 1102 46-47 -0.006905245798130011 1102 48-49 0.20645050964482436 1102 50-51 0.09540386086889896 1102 52-53 0.20856426165248365 1102 54-55 0.22060241234640188 1102 56-57 0.28818768537198025 1102 58-59 0.12517393368506902 1102 60-61 0.10075032015968333 1102 62-63 0.21601336419923456 1102 64-65 0.2507924390543721 1102 66-67 0.22876051828743016 1102 68-69 0.3326881571673326 1102 70-71 0.27268600226953765 1102 72-73 0.43017308583289093 1102 74-75 0.35864730048413307 1102 76-77 0.17796856081453427 1102 78-79 0.13173690975951402 1102 80-81 0.2545535435252546 1102 82-83 0.241587983389973 1102 84-85 0.12250345189556455 1102 86-87 0.1806179091232636 1102 88-89 0.3246424004641888 1102 90-91 0.2067808545498906 1102 92-93 0.3329511269569849 1102 94-95 0.30082160273166636 1102 96-97 0.2998280091410166 1102 98-99 0.3471438131655624 1102 100-101 0.9118927538410304 1103 1 -0.0019182640532022788 1103 2 -0.010231194009527655 1103 3 -0.034243425729286514 1103 4 -0.1007575757429251 1103 5 -0.07816765451623553 1103 6 -0.14225937769504782 1103 7 -0.09607303337637063 1103 8 -0.11210287232142235 1103 9 -0.0770051171565811 1103 10-11 -0.1134119614496214 1103 12-13 -0.11428049850334077 1103 14-15 -0.16962905800374273 1103 16-17 -0.25910478344448507 1103 18-19 -0.23103038627603922 1103 20-21 -0.27606837680408347 1103 22-23 -0.07389560311499821 1103 24-25 -0.1260709291806137 1103 26-27 -0.26334937331906616 1103 28-29 -0.35445737985801884 1103 30-31 -0.3111821120005658 1103 32-33 -0.21320331796019332 1103 34-35 -0.24664197788376896 1103 36-37 -0.4377987902435052 1103 38-39 -0.2910141738512877 1103 40-41 -0.2773946134436116 1103 42-43 -0.38916313218179965 1103 44-45 -0.39451911056578837 1103 46-47 -0.34053415390469155 1103 48-49 -0.3538784242208024 1103 50-51 -0.4001891703333911 1103 52-53 -0.4621447730724455 1103 54-55 -0.48339174127395523 1103 56-57 -0.4606399105868775 1103 58-59 -0.4843532775477897 1103 60-61 -0.4835843285771517 1103 62-63 -0.3978200299407817 1103 64-65 -0.5966087053881708 1103 66-67 -0.451890809133765 1103 68-69 -0.38850998039239926 1103 70-71 -0.331228959016876 1103 72-73 -0.2718545060402704 1103 74-75 -0.2610594452918775 1103 76-77 -0.08065569502171144 1103 78-79 -0.24096644073694407 1103 80-81 -0.4319096904521835 1103 82-83 -0.3592263953332413 1103 84-85 -0.42687261720892167 1103 86-87 -0.3919349248674493 1103 88-89 -0.13363567219677108 1103 90-91 -0.21516673742541315 1103 92-93 -0.2344044906714018 1103 94-95 -0.2837500484556301 1103 96-97 -0.10947450461709707 1103 98-99 0.024457316176690114 1103 100-101 0.6173979776303966 1104 1 0.1103053487961887 1104 2 0.09573459656202488 1104 3 0.10092553547221428 1104 4 0.04150358404013588 1104 5 0.0252950071441731 1104 6 0.07676440202961032 1104 7 -0.024316671257054168 1104 8 0.03766767419505612 1104 9 0.07985762794145757 1104 10-11 -3.539722965157921E-4 1104 12-13 0.00983297667396954 1104 14-15 0.029578284507735475 1104 16-17 -0.08910061156296933 1104 18-19 -0.006374566501314405 1104 20-21 0.03369382594935644 1104 22-23 -0.07806748463356428 1104 24-25 -0.019270762305346523 1104 26-27 -0.09313660736161467 1104 28-29 0.04020261179822171 1104 30-31 -0.11885837399472621 1104 32-33 -0.019419421422853134 1104 34-35 0.06374600709745692 1104 36-37 -0.07734822703950783 1104 38-39 -0.09848184176951946 1104 40-41 -0.052113011441107915 1104 42-43 -0.02141177632030633 1104 44-45 -0.003613812276256567 1104 46-47 -0.10377987772614006 1104 48-49 -0.04641075630257063 1104 50-51 -0.13256297091744784 1104 52-53 -0.11587860703156139 1104 54-55 0.01222778313155004 1104 56-57 -0.06681429523435156 1104 58-59 -0.20379424542429803 1104 60-61 -0.10540326891924678 1104 62-63 -0.028817109623723525 1104 64-65 -0.10495246842529582 1104 66-67 0.04956934939773561 1104 68-69 0.1446564776801864 1104 70-71 0.21257579692805706 1104 72-73 0.12635158490675025 1104 74-75 0.11274113877153624 1104 76-77 0.2047010008481287 1104 78-79 0.10717707198729443 1104 80-81 0.1515279399191165 1104 82-83 0.1092758992015952 1104 84-85 0.13841732855661704 1104 86-87 0.1581276533553293 1104 88-89 0.24475398153706251 1104 90-91 0.06518370062214629 1104 92-93 0.3803222510891331 1104 94-95 0.632186538945291 1104 96-97 0.7207299175773123 1104 98-99 0.8673859770027228 1104 100-101 1.343096767784754 1105 1 0.020310659272240628 1105 2 -0.023006936253075594 1105 3 -0.015158268643268968 1105 4 0.018866545737992624 1105 5 -0.07456097843837739 1105 6 -0.14986874726059796 1105 7 -0.13111328002441525 1105 8 -0.05712565755491994 1105 9 0.021749634029376352 1105 10-11 -0.04835026830618716 1105 12-13 -0.0055089619433346115 1105 14-15 -0.0623807474378566 1105 16-17 -0.12580551899434766 1105 18-19 -0.18871754497565263 1105 20-21 -0.06829595694379265 1105 22-23 -0.18368431377233208 1105 24-25 -0.18955326717773602 1105 26-27 0.0013406754536973153 1105 28-29 -0.14180626034384147 1105 30-31 -0.13278053860525318 1105 32-33 -0.17778466653646063 1105 34-35 -0.07226640358291547 1105 36-37 -0.04082860807727684 1105 38-39 0.007303819589331795 1105 40-41 0.012046434490336821 1105 42-43 -0.06558301737945271 1105 44-45 0.02375444210710498 1105 46-47 -0.020635969162391632 1105 48-49 0.16233925370460867 1105 50-51 0.14964729013202316 1105 52-53 -0.03137656868049277 1105 54-55 0.1765524142224777 1105 56-57 0.08341177804665278 1105 58-59 0.1301440552055695 1105 60-61 0.14178204534618288 1105 62-63 0.22902865173575293 1105 64-65 0.23827276069461334 1105 66-67 0.30680044761663083 1105 68-69 0.16877039762098178 1105 70-71 0.24738432179879055 1105 72-73 0.23335963423635775 1105 74-75 0.3619889102840794 1105 76-77 0.38842986235144394 1105 78-79 0.19016495751940354 1105 80-81 0.1636263073702544 1105 82-83 0.19866126274682117 1105 84-85 0.10943840767737356 1105 86-87 0.16054174061614646 1105 88-89 0.3142978232083742 1105 90-91 0.5451921155473407 1105 92-93 0.6017003418618039 1105 94-95 0.6989454495593925 1105 96-97 0.5261072860540814 1105 98-99 0.7005951323701751 1105 100-101 1.1908711268138532 1106 1 -0.07685626706670234 1106 2 0.008170467600848497 1106 3 0.08450398311938301 1106 4 -0.015827888593122452 1106 5 -0.0253839605281172 1106 6 -0.0671193834497501 1106 7 0.025449615959963978 1106 8 -0.03277983682099972 1106 9 -0.053639232731647724 1106 10-11 -0.08951943045671129 1106 12-13 -0.08941111856992023 1106 14-15 -0.006346860443301239 1106 16-17 -0.05257023689899398 1106 18-19 0.014437877200130345 1106 20-21 -0.01889345667524367 1106 22-23 0.040760274318358825 1106 24-25 -2.725197778659094E-4 1106 26-27 0.013396376820757894 1106 28-29 -0.104760041213396 1106 30-31 -0.05486193469467082 1106 32-33 -0.03909980284777248 1106 34-35 0.08723650858068055 1106 36-37 0.020317416465317706 1106 38-39 -0.16774539773401642 1106 40-41 -0.18848685220022077 1106 42-43 -0.3259794936947742 1106 44-45 -0.044977561843580816 1106 46-47 -0.13315537328819715 1106 48-49 -0.046900389744280346 1106 50-51 0.017841634631430736 1106 52-53 0.052853354881747805 1106 54-55 -0.03226487757469698 1106 56-57 -0.10061053444033519 1106 58-59 0.030457087838726693 1106 60-61 0.037931829512423576 1106 62-63 0.13884844646315742 1106 64-65 0.20274778700246543 1106 66-67 -0.007172424155960755 1106 68-69 -0.03237925821422749 1106 70-71 0.03236902188918833 1106 72-73 0.09816832120087327 1106 74-75 0.06967422740904539 1106 76-77 0.2793177894442138 1106 78-79 0.3107798627398175 1106 80-81 0.13580452208611504 1106 82-83 0.02778936277277566 1106 84-85 0.17013860074426645 1106 86-87 0.19680181341310643 1106 88-89 0.4156048984303169 1106 90-91 0.4105525308455391 1106 92-93 0.5224162008193574 1106 94-95 0.48138957565840457 1106 96-97 0.6151358392311259 1106 98-99 0.7989664691136262 1106 100-101 1.169160369727365 1107 1 -0.0354894173475131 1107 2 -0.03572775012511897 1107 3 0.014833914594028386 1107 4 -0.02469758847814063 1107 5 -0.05684865852381904 1107 6 -0.07367678769105623 1107 7 -0.018510298045534057 1107 8 -9.618216805051816E-5 1107 9 -0.036921361524356655 1107 10-11 -0.03655479274130613 1107 12-13 -0.03926509055617089 1107 14-15 -0.06227200930327115 1107 16-17 -0.03119095891942436 1107 18-19 0.007968263730354863 1107 20-21 0.017097725409286113 1107 22-23 0.049270112953259115 1107 24-25 -0.001517267502791242 1107 26-27 -0.04708832937953389 1107 28-29 0.0333358538195867 1107 30-31 0.02068718242145451 1107 32-33 -0.04121750235735533 1107 34-35 -0.06432687514266888 1107 36-37 -0.16892358174407462 1107 38-39 -0.1789866621476861 1107 40-41 -0.18008311093782936 1107 42-43 -0.27190250240182934 1107 44-45 -0.17745454407425143 1107 46-47 -0.3727952531159744 1107 48-49 -0.28658052386592914 1107 50-51 -0.30330034529725935 1107 52-53 -0.39499215035211677 1107 54-55 -0.3521284296783236 1107 56-57 -0.2567960820565105 1107 58-59 -0.3566460028009928 1107 60-61 -0.1527091685351465 1107 62-63 -0.30102215722178727 1107 64-65 -0.2296942812024625 1107 66-67 -0.3445398176115404 1107 68-69 -0.2944568612106053 1107 70-71 -0.19083132797928926 1107 72-73 -0.12858927431913258 1107 74-75 -0.1195567577976 1107 76-77 -0.10411315886603845 1107 78-79 -0.04271584845848153 1107 80-81 -0.18892787788702137 1107 82-83 -0.40209787261087016 1107 84-85 -0.3162972193469571 1107 86-87 -0.20876575999333014 1107 88-89 -0.16205008464669746 1107 90-91 -0.0702509246315941 1107 92-93 0.07255929573129549 1107 94-95 0.032163692026085045 1107 96-97 0.31855656416443523 1107 98-99 0.4172120413435856 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2316 22-23 0.2394397550645948 2316 24-25 0.486443318929382 2316 26-27 0.3930961836271223 2316 28-29 0.49529990042434235 2316 30-31 0.4597877020801491 2316 32-33 0.38381736514612896 2316 34-35 0.31526158706753904 2316 36-37 0.3016532444651503 2316 38-39 0.2866482807860464 2316 40-41 0.19127700013194016 2316 42-43 0.2799769889249504 2316 44-45 0.26937124750034513 2316 46-47 0.16340556851681498 2316 48-49 -0.48317882238237786 2316 50-51 -0.26576905313667964 2316 52-53 0.16596428235423133 2316 54-55 -0.020565998495762017 2316 56-57 0.0316901114245951 2316 58-59 0.04428345979648185 2316 60-61 0.25285400176255024 2316 62-63 0.21206802748105957 2316 64-65 -0.04921508400299501 2316 66-67 -0.13067862444684408 2316 68-69 0.02871395538506505 2316 70-71 0.04477364535435413 2316 72-73 0.36905384697421795 2316 74-75 0.31638132359112703 2316 76-77 0.3195551682784732 2316 78-79 0.21250573420977403 2316 80-81 0.33757901841158144 2316 82-83 0.41371153440110575 2316 84-85 0.5586148208892574 2316 86-87 0.2195625362197191 2316 88-89 -0.020589901943509403 2316 90-91 0.16547206665849146 2316 92-93 0.4160782431503378 2316 94-95 0.27469818617517916 2316 96-97 0.36917645901771223 2316 98-99 0.6355114057551923 2316 100-101 0.24315751317255518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 25.0 5 53.0 6 89.0 7 224.0 8 513.0 9 998.0 10 1635.0 11 2686.0 12 4336.0 13 6131.0 14 7795.0 15 11264.0 16 15931.0 17 23804.0 18 21658.0 19 12447.0 20 10128.0 21 9946.0 22 10288.0 23 11048.0 24 12481.0 25 14708.0 26 17987.0 27 22245.0 28 27993.0 29 34723.0 30 43769.0 31 55281.0 32 70582.0 33 93794.0 34 129597.0 35 186515.0 36 291926.0 37 460207.0 38 396660.0 39 39465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.214225358579185 19.91622488382748 15.302097406155948 21.567452351437392 2 15.621728492914144 21.177525365542493 39.8087597446681 23.391986396875264 3 19.219147842714847 25.802792759771997 30.24045104275819 24.73760835475497 4 12.81720654718706 17.506165380067067 37.21215008143231 32.464477991313565 5 13.146744952767223 38.39630630168945 33.823060722975065 14.63388802256826 6 30.891567887996775 37.10277650747386 17.483958786514595 14.521696818014771 7 27.993094969547684 33.892630388088286 20.4353370838887 17.67893755847533 8 26.89008614694127 35.037075250092606 19.370006261771326 18.702832341194792 9 26.944797336770556 16.657775894962324 20.21712679867307 36.180299969594046 10-11 24.761327692039636 27.677910372148705 26.66655766716335 20.894204268648313 12-13 25.92777882172639 24.984174010235037 27.83141233231406 21.256634835724515 14-15 22.8866277750311 25.9567503407858 26.651572690477664 24.50504919370543 16-17 21.420406932166422 29.579353431846013 27.178991488773956 21.82124814721361 18-19 21.48949140067967 27.38568362876237 30.466738671342902 20.658086299215057 20-21 22.388736425304582 26.360861537740714 30.65478703388726 20.59561500306744 22-23 21.980818149694763 26.411478069379125 30.491781995943224 21.115921784982888 24-25 21.637541637688056 26.58225654916603 30.810546994155473 20.969654818990445 26-27 21.482817499178232 26.839831804934327 30.63212734643757 21.04522334944987 28-29 21.323837688012265 26.95689670740296 30.731125318407376 20.988140286177398 30-31 21.499132596672947 26.712850087960483 30.708176432447836 21.07984088291873 32-33 21.39524759375697 26.823002712132755 30.490848687199872 21.2909010069104 34-35 21.699185091225175 26.75462592627118 30.50712538818834 21.03906359431531 36-37 21.745650247677027 26.74577592250817 30.35932996524786 21.149243864566944 38-39 21.58894464709804 26.865660350837462 30.463008424901634 21.082386577162865 40-41 21.742342067535002 26.81670377939786 30.37923313912699 21.06172101394015 42-43 21.47925874764179 26.962798269142063 30.18497140862842 21.372971574587726 44-45 21.669257443501134 27.108253800144954 29.869151534821746 21.35333722153216 46-47 21.874129123288565 27.347712794072642 29.247157434011033 21.531000648627753 48-49 21.939089510142068 27.430340844367894 29.032265936403245 21.598303709086792 50-51 22.13318977950073 27.6686834808811 28.79026287238139 21.40786386723678 52-53 22.17118501704051 27.81876100873573 28.519684070942013 21.490369903281746 54-55 21.8911135069287 27.881110290633064 28.589036970805893 21.63873923163234 56-57 21.928132630466177 28.181924223293887 28.392301193641007 21.49764195259893 58-59 22.007881144454622 27.703286722262867 28.5791782193826 21.709653913899913 60-61 21.9339405087799 27.80416810440125 28.813006328635133 21.448885058183713 62-63 21.868418856375076 27.84321266449351 28.93404446492115 21.35432401421027 64-65 21.735648253328975 27.66317518737123 28.900595210061002 21.70058134923879 66-67 21.675465520969126 28.00846876508401 28.573638772419507 21.742426941527356 68-69 21.78418021797602 27.923498041183226 28.60797358245064 21.684348158390115 70-71 21.82324918091834 27.914883835112867 28.520489364993917 21.741377618974873 72-73 21.810510893188237 27.91837344267112 28.271507085855575 21.999608578285077 74-75 21.745038046483526 28.219333792432288 28.053125980689536 21.982502180394654 76-77 21.90116748115246 28.01505753459958 28.234829602218902 21.848945382029058 78-79 21.829349893432752 28.03855747920511 28.08960824152574 22.042484385836392 80-81 21.80990614417549 28.30470186908795 27.94888382556741 21.93650816116915 82-83 21.84444826757348 28.342758973270225 27.919172544727562 21.893620214428733 84-85 21.833312102750167 28.016731651773537 27.90377030754086 22.24618593793544 86-87 21.716584323316287 27.994388320600738 28.015252757400045 22.27377459868293 88-89 21.86605177991948 27.815222595477373 28.089315407325056 22.229410217278094 90-91 21.81101122745449 27.87076163898139 28.01892352616018 22.299303607403946 92-93 21.622176967200414 27.726846219829586 28.12054864053942 22.53042817243058 94-95 21.519384892000307 27.72844606067232 28.198322938532637 22.55384610879474 96-97 21.510307031778854 27.848508082956023 28.03797181080373 22.603213074461394 98-99 21.323778464746127 27.789347457072882 27.93056695712608 22.95630712105491 100-101 21.324464359366722 28.02097900391221 27.93048325413735 22.72407338258372 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 699.0 1 492.0 2 361.5 3 479.5 4 456.0 5 448.0 6 758.5 7 1585.0 8 2647.0 9 3343.0 10 3358.5 11 3147.5 12 3302.0 13 3595.0 14 3920.5 15 4214.0 16 4354.5 17 4469.5 18 4686.5 19 4922.5 20 5193.0 21 5785.5 22 6630.5 23 7648.0 24 8895.5 25 10712.5 26 13262.5 27 16770.0 28 20128.0 29 23923.0 30 28500.0 31 31591.5 32 34709.0 33 38668.5 34 43330.0 35 47688.5 36 53104.5 37 57946.5 38 60261.0 39 62543.0 40 64049.5 41 65583.0 42 67588.5 43 70572.0 44 73492.5 45 74212.5 46 73871.0 47 75041.0 48 79052.0 49 78988.5 50 74588.5 51 70746.5 52 67371.0 53 64800.5 54 62681.5 55 58110.5 56 50641.5 57 44497.0 58 40673.5 59 36240.5 60 30286.0 61 24466.5 62 19196.5 63 14544.5 64 10633.0 65 7995.5 66 5771.5 67 4329.0 68 3229.0 69 2101.0 70 1456.5 71 1011.0 72 800.5 73 678.0 74 497.5 75 350.5 76 249.5 77 144.0 78 70.5 79 42.0 80 31.0 81 23.5 82 18.0 83 11.0 84 5.0 85 3.5 86 4.5 87 3.5 88 1.5 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07589286367933484 2 0.0 3 0.0 4 0.0 5 0.002245062205305082 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.761140023065573E-5 12-13 0.004685347211071476 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001220142502883197 24-25 7.808912018452458E-4 26-27 5.124598512109426E-4 28-29 0.0035140104083036068 30-31 4.6365415109561473E-4 32-33 0.0 34-35 0.0 36-37 1.2201425028831968E-4 38-39 7.320855017299181E-4 40-41 1.7081995040364755E-4 42-43 0.012006202228370656 44-45 2.9283420069196723E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.4402850057663933E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.4402850057663933E-5 82-83 4.148484509802869E-4 84-85 4.880570011532787E-4 86-87 0.0 88-89 0.0 90-91 0.008223760469432745 92-93 0.0028551334567466806 94-95 0.0 96-97 0.0 98-99 1.4641710034598362E-4 100-101 0.011030088226064098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2048941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.26224439965892 #Duplication Level Percentage of deduplicated Percentage of total 1 76.16933121707132 41.331188662592936 2 13.291411082180222 14.424435931151963 3 4.351251900948963 7.083260822813194 4 1.9854516556813089 4.309402519371465 5 1.0656651790246223 2.891269219622016 6 0.6662018439156013 2.168976436443107 7 0.43484481457708246 1.6516958923154208 8 0.3220598676468197 1.398055299965883 9 0.23361215703890953 1.1408687963979127 >10 1.3349676488460482 13.373719362264309 >50 0.09929486240862365 3.6923460186694004 >100 0.04357309538063673 4.105504070539139 >500 0.0016163136552846992 0.5832856887131522 >1k 4.4897601535685833E-4 0.394963979008336 >5k 8.979520307137165E-5 0.3285032255704666 >10k+ 1.795904061427433E-4 1.1225240745613183 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12315 0.6010421969202627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10723 0.5233435223366607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6742 0.32904803017754053 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 0.13304433851438377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.320855017299181E-5 0.0 18-19 0.0 0.0 0.0 9.761140023065574E-5 0.0 20-21 0.0 0.0 0.0 9.761140023065574E-5 0.0 22-23 0.0 0.0 0.0 1.952228004613115E-4 0.0 24-25 0.0 0.0 0.0 4.636541510956148E-4 0.0 26-27 0.0 0.0 0.0 7.320855017299181E-4 0.0 28-29 0.0 0.0 0.0 0.0017326023540941392 0.0 30-31 0.0 0.0 0.0 0.003758038908880246 0.0 32-33 0.0 0.0 0.0 0.006661978065742254 0.0 34-35 0.0 0.0 0.0 0.010468822674737827 0.0 36-37 0.0 0.0 0.0 0.014397681534021722 0.0 38-39 0.0 0.0 0.0 0.018863403094574224 0.0 40-41 0.0 0.0 0.0 0.024939712758932545 0.0 42-43 0.0 0.0 0.0 0.03352951597923025 0.0 44-45 0.0 0.0 0.0 0.046511832209907455 0.0 46-47 0.0 0.0 0.0 0.07569764087887351 4.880570011532787E-5 48-49 0.0 0.0 0.0 0.13485014941865092 4.880570011532787E-5 50-51 0.0 0.0 0.0 0.17804319402071606 9.761140023065574E-5 52-53 0.0 0.0 0.0 0.22052855597110899 9.761140023065574E-5 54-55 0.0 0.0 0.0 0.2788025619088105 9.761140023065574E-5 56-57 0.0 0.0 0.0 0.32611968817062087 9.761140023065574E-5 58-59 0.0 0.0 0.0 0.384198471307861 9.761140023065574E-5 60-61 0.0 0.0 0.0 0.4747818507219095 9.761140023065574E-5 62-63 0.0 0.0 0.0 0.5414504370794474 9.761140023065574E-5 64-65 0.0 0.0 0.0 0.6191979173631648 9.761140023065574E-5 66-67 0.0 0.0 0.0 0.7519006159767412 1.2201425028831968E-4 68-69 0.0 0.0 0.0 0.8942912460632102 1.4641710034598362E-4 70-71 0.0 0.0 0.0 1.0276528216283436 1.4641710034598362E-4 72-73 0.0 0.0 0.0 1.1881503664575992 1.4641710034598362E-4 74-75 0.0 0.0 0.0 1.3366905147585997 1.4641710034598362E-4 76-77 0.0 0.0 0.0 1.467733819568255 1.4641710034598362E-4 78-79 0.0 0.0 0.0 1.638016907270634 1.4641710034598362E-4 80-81 0.0 0.0 0.0 1.8865843379579987 1.4641710034598362E-4 82-83 0.0 0.0 0.0 2.118655442006383 1.4641710034598362E-4 84-85 0.0 0.0 0.0 2.3719814284549923 1.4641710034598362E-4 86-87 0.0 0.0 0.0 2.783364674727091 1.4641710034598362E-4 88-89 0.0 0.0 0.0 3.335918408582775 1.4641710034598362E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7935 0.0 41.15481 1 GTATCAA 12930 0.0 35.255825 1 TATCAAC 16045 0.0 28.329123 2 ATCAACG 16025 0.0 28.305202 3 TCAACGC 16265 0.0 27.916742 4 CAACGCA 16490 0.0 27.507025 5 AACGCAG 16890 0.0 26.883709 6 TACCTGG 1950 0.0 25.331427 2 GTACATG 11575 0.0 24.722153 1 GTACCTG 2120 0.0 24.440014 1 TACATGG 11725 0.0 23.859612 2 ACGCAGA 19110 0.0 23.664688 7 CGCAGAG 19145 0.0 23.522175 8 GAGTACT 10640 0.0 22.858768 12-13 ACATGGG 11765 0.0 22.84996 3 ACCGTCG 125 2.255653E-4 22.801624 8 AGTACTT 10925 0.0 21.827639 12-13 GCAGAGT 20395 0.0 21.754427 9 GTACTTT 11150 0.0 21.53313 14-15 GGACCGA 500 0.0 19.9485 6 >>END_MODULE