FastQCFastQC Report
Thu 26 May 2016
SRR937395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251085
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.23628605761221813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.17640382304533148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33180.14739558923807852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.119808892156449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.051.339061
GTATCAA54900.041.2970161
GTACGGT550.002578218534.5507246
ATCAACG68450.032.831163
TCAACGC69400.032.1756444
TATCAAC70800.031.7414262
CAACGCA70150.031.6976015
AACGCAG71500.031.0956546
GTACATG64850.026.3121971
ACGCAGA84350.026.0774697
TACATGG62700.025.6880252
CGCAGAG84600.025.663478
ACATGGG65400.023.6830923
GCAGAGT96350.022.1886229
AGAGTAC84350.020.61254910-11
GAGTACT57500.020.44649512-13
CAGAGTA89200.019.91784910-11
CATGGGG42450.019.5861574
GTACTTT60500.019.11770614-15
AGTACTT60750.018.96168912-13
Produced by FastQC (version 0.11.5)