##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2251085 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.693828975805 33.0 31.0 34.0 30.0 34.0 2 31.814799085774194 33.0 31.0 34.0 30.0 34.0 3 31.85635016003394 33.0 31.0 34.0 30.0 34.0 4 35.46434319450398 37.0 35.0 37.0 33.0 37.0 5 35.464884711150404 37.0 35.0 37.0 33.0 37.0 6 35.48936846009813 37.0 35.0 37.0 33.0 37.0 7 35.41830006419127 37.0 35.0 37.0 33.0 37.0 8 35.39765313171204 37.0 35.0 37.0 33.0 37.0 9 37.00820670920911 39.0 37.0 39.0 33.0 39.0 10-11 36.882384272473054 39.0 37.0 39.0 33.0 39.0 12-13 36.73722915838363 39.0 37.0 39.0 33.0 39.0 14-15 37.869353000886235 40.0 38.0 41.0 33.0 41.0 16-17 37.778297132271774 40.0 38.0 41.0 33.0 41.0 18-19 37.670258119973255 40.0 37.0 41.0 32.0 41.0 20-21 37.51452921591144 40.0 37.0 41.0 32.0 41.0 22-23 37.58656336833127 40.0 37.0 41.0 32.0 41.0 24-25 37.558329427809255 40.0 37.0 41.0 32.0 41.0 26-27 37.4661347750085 40.0 37.0 41.0 32.0 41.0 28-29 37.32720954561911 40.0 37.0 41.0 32.0 41.0 30-31 37.09195210309695 39.0 36.5 41.0 31.0 41.0 32-33 36.86004904301703 39.0 36.0 41.0 30.5 41.0 34-35 36.906561724679435 39.0 36.0 41.0 30.5 41.0 36-37 36.79415748405769 39.0 36.0 41.0 30.0 41.0 38-39 36.65304864098868 39.0 36.0 41.0 30.0 41.0 40-41 36.445165553499756 39.0 35.5 41.0 30.0 41.0 42-43 36.19635486887434 39.0 35.0 41.0 30.0 41.0 44-45 35.992422542907086 39.0 35.0 40.0 29.0 41.0 46-47 35.65829122400976 39.0 35.0 40.0 27.5 41.0 48-49 35.35704871206551 39.0 35.0 40.0 26.5 41.0 50-51 34.40156568943421 37.5 33.5 39.5 25.0 40.5 52-53 34.51849374857014 38.0 34.0 39.5 26.0 40.5 54-55 34.823797857477615 38.0 34.0 40.0 26.0 41.0 56-57 34.544000337614975 38.0 34.0 40.0 24.5 41.0 58-59 34.258732344624924 38.0 34.0 40.0 23.5 41.0 60-61 33.90951874318384 37.5 33.0 40.0 22.5 41.0 62-63 33.50995364457584 37.0 33.0 40.0 22.0 41.0 64-65 33.06097948322698 36.5 32.0 39.0 20.5 41.0 66-67 32.60627030965068 36.0 31.5 39.0 18.5 40.5 68-69 32.078313568790165 35.5 31.0 39.0 17.0 40.0 70-71 31.59554281601983 35.0 31.0 38.0 13.0 40.0 72-73 31.08978981246821 35.0 30.0 37.5 8.0 39.5 74-75 30.516373215582707 34.5 29.5 37.0 7.0 39.0 76-77 29.967271338043652 34.0 29.0 36.0 2.0 39.0 78-79 29.392338805509343 34.0 29.0 36.0 2.0 38.0 80-81 28.803590935037995 34.0 28.0 35.0 2.0 37.0 82-83 28.218522623534874 33.5 26.5 35.0 2.0 37.0 84-85 27.605664601736496 33.0 26.0 35.0 2.0 36.0 86-87 26.96482029776752 32.5 25.5 35.0 2.0 36.0 88-89 26.37639049613853 32.0 24.5 35.0 2.0 35.0 90-91 25.779165824480195 32.0 22.5 34.0 2.0 35.0 92-93 25.060682071090163 31.5 19.5 34.0 2.0 35.0 94-95 24.230868669996912 31.0 12.0 34.0 2.0 35.0 96-97 23.303375483378016 31.0 2.0 34.0 2.0 35.0 98-99 22.049985007229846 30.0 2.0 34.0 2.0 35.0 100-101 18.972162312840254 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07164297113647677 1101 2 0.1785583891533271 1101 3 0.09289996977431514 1101 4 0.014696247624335967 1101 5 0.07391920786574957 1101 6 0.05141906507268601 1101 7 0.1638962504182686 1101 8 0.07359986330297374 1101 9 0.05302676839210818 1101 10-11 0.16397273025719983 1101 12-13 0.21524898954627503 1101 14-15 0.2544755395937841 1101 16-17 0.29670019317129004 1101 18-19 0.2846742505493225 1101 20-21 0.33962811846606655 1101 22-23 0.2952858256838766 1101 24-25 0.3135357782052921 1101 26-27 0.26968425352292513 1101 28-29 0.23984666311207548 1101 30-31 0.23419475479214924 1101 32-33 0.23957211787067223 1101 34-35 0.41315344635878404 1101 36-37 0.38343305620731627 1101 38-39 0.4100841030063478 1101 40-41 0.6110301072262914 1101 42-43 0.6525959183945247 1101 44-45 0.5620867368703273 1101 46-47 0.6496962886645079 1101 48-49 0.7599938168795504 1101 50-51 0.7235846200005867 1101 52-53 0.6921155063063722 1101 54-55 0.8559888386127881 1101 56-57 0.9186349555865831 1101 58-59 0.8515652522707242 1101 60-61 1.0517575588185437 1101 62-63 0.9533140625974568 1101 64-65 0.8453929604238724 1101 66-67 1.0548540892843477 1101 68-69 1.0427694317142375 1101 70-71 1.021871877213723 1101 72-73 0.9888803013810552 1101 74-75 1.1198427849357309 1101 76-77 1.1559313127777884 1101 78-79 1.2667879075394985 1101 80-81 1.362705461745005 1101 82-83 1.3908352630686274 1101 84-85 1.5689245792883035 1101 86-87 1.5332891389298595 1101 88-89 1.5941222932890184 1101 90-91 1.3782970060404836 1101 92-93 1.6011552971060077 1101 94-95 1.7173656533092831 1101 96-97 1.9396766632305535 1101 98-99 1.9569191003370712 1101 100-101 1.934800409590519 1102 1 0.1483030403766783 1102 2 0.13814334129963868 1102 3 0.0726551478402655 1102 4 0.10576635973291104 1102 5 0.10089117982877838 1102 6 0.06572312398983371 1102 7 -0.08553057208354886 1102 8 -0.0435899786865761 1102 9 0.10777646846845101 1102 10-11 0.08400038938658838 1102 12-13 0.0759484816378162 1102 14-15 0.25455813158576746 1102 16-17 0.3200884772464292 1102 18-19 0.36756055410523203 1102 20-21 0.2264073605506951 1102 22-23 0.25381794100633215 1102 24-25 0.390846749818877 1102 26-27 0.3897058138569989 1102 28-29 0.28681339646817605 1102 30-31 0.36395551514306845 1102 32-33 0.3799729914883372 1102 34-35 0.43778741434427815 1102 36-37 0.48719343036741236 1102 38-39 0.291053149931507 1102 40-41 0.4767494839731157 1102 42-43 0.5208108844409196 1102 44-45 0.49236712650188963 1102 46-47 0.5910561730138753 1102 48-49 0.5378741020965734 1102 50-51 0.398011656059559 1102 52-53 0.3986672017416737 1102 54-55 0.5999769064290135 1102 56-57 0.5848547414692007 1102 58-59 0.5971935881081407 1102 60-61 0.5994959785074414 1102 62-63 0.48585491012340043 1102 64-65 0.37569885772055756 1102 66-67 0.45165298657447295 1102 68-69 0.6566180476949519 1102 70-71 0.6771581322176665 1102 72-73 0.728341205619838 1102 74-75 0.8221824874376864 1102 76-77 0.7926796635701621 1102 78-79 0.7504210354010397 1102 80-81 0.6851282091787958 1102 82-83 0.8479772702397774 1102 84-85 0.9930471722259 1102 86-87 1.0917046719059655 1102 88-89 0.9749587361140009 1102 90-91 1.1085367105488864 1102 92-93 1.1111931791672234 1102 94-95 1.252979141460635 1102 96-97 1.1413100874895754 1102 98-99 1.3386904269005448 1102 100-101 1.4509265581059196 1103 1 0.37883004795790143 1103 2 0.333109911000971 1103 3 0.3666714880262134 1103 4 0.33272918265680573 1103 5 0.4091512947765352 1103 6 0.31803613063809877 1103 7 0.4055246889285442 1103 8 0.3646901094061974 1103 9 0.3775899272041343 1103 10-11 0.3757155881589327 1103 12-13 0.3241869337432419 1103 14-15 0.4259142575954371 1103 16-17 0.40982975141002953 1103 18-19 0.46371059307374196 1103 20-21 0.42303923790987596 1103 22-23 0.3050602091030612 1103 24-25 0.4015090913877799 1103 26-27 0.320341926665499 1103 28-29 0.22831070338949644 1103 30-31 0.3439652863835434 1103 32-33 0.3362426254304651 1103 34-35 0.6128767496736103 1103 36-37 0.5206368027659352 1103 38-39 0.5433236944205362 1103 40-41 0.7610358373492474 1103 42-43 0.4784400215900675 1103 44-45 0.7160291619916848 1103 46-47 0.7302048336376217 1103 48-49 0.6853825895845702 1103 50-51 0.5806208035841962 1103 52-53 0.5166011523748253 1103 54-55 0.8173484449390571 1103 56-57 0.7802083595198326 1103 58-59 0.6644944625539608 1103 60-61 0.6546011495305208 1103 62-63 0.7045883139010769 1103 64-65 0.6027764328445286 1103 66-67 0.8462861321953952 1103 68-69 0.937726330925134 1103 70-71 0.9346515222931835 1103 72-73 0.8245438141697043 1103 74-75 0.7698012666764278 1103 76-77 0.6533888947886481 1103 78-79 0.7401731530707671 1103 80-81 0.794034144454816 1103 82-83 0.9292108964905985 1103 84-85 1.1812645657370666 1103 86-87 1.1743605332989304 1103 88-89 1.267837716653041 1103 90-91 1.2295781990997021 1103 92-93 1.2975431715097514 1103 94-95 1.36487907095038 1103 96-97 1.5240101578679948 1103 98-99 1.4144700002838348 1103 100-101 1.2645158113673745 1104 1 0.14903382387829822 1104 2 0.20753669682015996 1104 3 0.1443114837143895 1104 4 0.18408638640273978 1104 5 0.20525007740042867 1104 6 0.12793106956529954 1104 7 0.2719864214580596 1104 8 0.25004749619273525 1104 9 0.23881649769979418 1104 10-11 0.3429882730714695 1104 12-13 0.2827258394034189 1104 14-15 0.5150678530781434 1104 16-17 0.3656447296831544 1104 18-19 0.3227322599761706 1104 20-21 0.3516105390822375 1104 22-23 0.45045096903037063 1104 24-25 0.312771573291343 1104 26-27 0.3917055803076295 1104 28-29 0.39516570568754616 1104 30-31 0.4518204595715005 1104 32-33 0.3394997338325325 1104 34-35 0.4607477655723926 1104 36-37 0.6580140738712572 1104 38-39 0.6440211103486604 1104 40-41 0.652926224339673 1104 42-43 0.6728373960609417 1104 44-45 0.6752376930662933 1104 46-47 0.6783433652796234 1104 48-49 0.6506511359066067 1104 50-51 0.4648990645468203 1104 52-53 0.6643154801626565 1104 54-55 0.8064496141137312 1104 56-57 0.7519348281838205 1104 58-59 0.9303851908783329 1104 60-61 0.9883307178456349 1104 62-63 0.9255040587041137 1104 64-65 0.9195585811905786 1104 66-67 1.088456882328586 1104 68-69 0.9711355893241809 1104 70-71 0.9093617646636964 1104 72-73 0.9783459142764208 1104 74-75 0.8506985825676203 1104 76-77 0.9676848901789725 1104 78-79 1.0029787776567112 1104 80-81 0.9820570350093547 1104 82-83 1.034724944634295 1104 84-85 1.039595538604587 1104 86-87 1.0287659428288833 1104 88-89 1.1405136889615157 1104 90-91 1.2336669049033944 1104 92-93 1.3375797372711027 1104 94-95 1.2267990108613347 1104 96-97 1.3359075632729542 1104 98-99 1.7107246795519906 1104 100-101 1.6522310443255641 1105 1 0.061575596184731296 1105 2 -0.07470191622320144 1105 3 0.11214422856007289 1105 4 0.11315320575809551 1105 5 0.11119425403785499 1105 6 0.12528137060101585 1105 7 0.08231618517747563 1105 8 0.10833921841968674 1105 9 0.1752513414990915 1105 10-11 0.09822915538168075 1105 12-13 0.24114326542979114 1105 14-15 0.25996237881040685 1105 16-17 0.171906825060276 1105 18-19 0.19059706213857197 1105 20-21 0.1791952636794747 1105 22-23 0.2526472767324677 1105 24-25 0.14428237885313422 1105 26-27 0.15721356250426766 1105 28-29 0.12914134599563454 1105 30-31 0.1847296003451433 1105 32-33 0.24544192233600626 1105 34-35 0.2668782679749171 1105 36-37 0.3684114126518665 1105 38-39 0.409857833181718 1105 40-41 0.34582223483186425 1105 42-43 0.4052119974669566 1105 44-45 0.4253035164202643 1105 46-47 0.49042967221655687 1105 48-49 0.4332401441265219 1105 50-51 0.41956509673008924 1105 52-53 0.34631828977207135 1105 54-55 0.4896500863654012 1105 56-57 0.4389374185611672 1105 58-59 0.43140886493269903 1105 60-61 0.4669386114037408 1105 62-63 0.4561695204541465 1105 64-65 0.5342714924978793 1105 66-67 0.7494270616088272 1105 68-69 0.7117511134478782 1105 70-71 0.7312764386828015 1105 72-73 0.6956798524323453 1105 74-75 0.6522748611419367 1105 76-77 0.49719787903424617 1105 78-79 0.7210980824589264 1105 80-81 0.8900535569570636 1105 82-83 0.9431403180466873 1105 84-85 1.0553072776651184 1105 86-87 1.0106028345995988 1105 88-89 1.107681752265421 1105 90-91 1.246711105527389 1105 92-93 1.4097968763183992 1105 94-95 1.5351842183709365 1105 96-97 1.459515976969886 1105 98-99 1.6630703444742565 1105 100-101 1.5377404278879752 1106 1 -0.05233206898643772 1106 2 -0.5746412234793077 1106 3 -0.121149287716797 1106 4 0.01950416175991876 1106 5 -0.054417461539969736 1106 6 -0.09005704541088022 1106 7 0.06639043721299487 1106 8 0.04208755182217061 1106 9 0.06203975061472278 1106 10-11 -0.4045623604175006 1106 12-13 -0.28506695909691615 1106 14-15 -0.14418296363123062 1106 16-17 -0.11314533705915153 1106 18-19 0.050189024681202454 1106 20-21 -0.007165380734491578 1106 22-23 -0.029355250216234197 1106 24-25 0.03339028802875532 1106 26-27 0.03929709254002489 1106 28-29 0.25079595634541363 1106 30-31 0.18973696196848522 1106 32-33 0.20324203323692558 1106 34-35 0.2626266922062612 1106 36-37 0.0332636980069978 1106 38-39 0.28382689754349855 1106 40-41 0.4391366277306119 1106 42-43 0.21390987038952147 1106 44-45 0.27477591005238367 1106 46-47 0.38646650612420075 1106 48-49 0.49024485769601966 1106 50-51 0.5510564169668655 1106 52-53 0.4732664848007033 1106 54-55 0.47104804069753925 1106 56-57 0.3377616333119988 1106 58-59 0.008460405827548811 1106 60-61 0.2540365955636261 1106 62-63 0.3442394155075519 1106 64-65 0.520018439358509 1106 66-67 0.7877320324146382 1106 68-69 0.806384767560349 1106 70-71 0.6396190417931926 1106 72-73 0.6400354693004964 1106 74-75 0.5454822638064769 1106 76-77 0.8398561455261309 1106 78-79 0.8019050044032916 1106 80-81 0.9619279927808648 1106 82-83 0.9287571189195418 1106 84-85 0.7630630253941177 1106 86-87 0.6867987059231311 1106 88-89 1.195047345170451 1106 90-91 0.8948161182714642 1106 92-93 1.0746095642136027 1106 94-95 1.2682888582681322 1106 96-97 1.360568302244868 1106 98-99 1.291146821816941 1106 100-101 1.1092203608764564 1107 1 0.10360705143712323 1107 2 0.16746767565270915 1107 3 0.08797549486263989 1107 4 0.1171222812213415 1107 5 0.016456337228468954 1107 6 0.11021525698270551 1107 7 0.20867684973884337 1107 8 0.021148943689659916 1107 9 0.09517679665362522 1107 10-11 0.13219330949246455 1107 12-13 0.2101290998389871 1107 14-15 0.2674472101294043 1107 16-17 0.3054408548481078 1107 18-19 0.36382231705146495 1107 20-21 0.310648540001651 1107 22-23 0.25175498754137493 1107 24-25 0.40836186951667486 1107 26-27 0.33470547944162377 1107 28-29 0.3504154211013102 1107 30-31 0.27161781883437186 1107 32-33 0.1842141342910324 1107 34-35 0.3387958828409765 1107 36-37 0.3649438822862692 1107 38-39 0.5569446371820348 1107 40-41 0.4975553883742947 1107 42-43 0.41545744403969564 1107 44-45 0.43846578178560947 1107 46-47 0.5502744473022787 1107 48-49 0.5081145696550706 1107 50-51 0.39879237852503735 1107 52-53 0.5058364489039491 1107 54-55 0.5971917531888948 1107 56-57 0.724800245506465 1107 58-59 0.8539114432320716 1107 60-61 0.8080946681489038 1107 62-63 0.7776032336945562 1107 64-65 0.6728553192211066 1107 66-67 0.9177241419200328 1107 68-69 1.079318506806036 1107 70-71 1.0135324694567807 1107 72-73 1.1227339760175745 1107 74-75 0.9513030116543995 1107 76-77 0.9593916824586763 1107 78-79 1.027037063334344 1107 80-81 1.078931629744151 1107 82-83 1.0291063710655557 1107 84-85 0.9156034433387354 1107 86-87 0.8810985738187327 1107 88-89 0.9847053408817565 1107 90-91 1.1949023438262394 1107 92-93 1.3610696058152705 1107 94-95 1.3482793914933886 1107 96-97 1.3335401753446128 1107 98-99 1.4379309375207505 1107 100-101 1.287680204836846 1108 1 0.31460071321104977 1108 2 0.3502112762365108 1108 3 0.35958096727928535 1108 4 0.28242257435708495 1108 5 0.30431530902002635 1108 6 0.363830625306079 1108 7 0.32175847531129165 1108 8 0.19461307143981088 1108 9 0.30471899559200466 1108 10-11 0.446541838580643 1108 12-13 0.45728746731105474 1108 14-15 0.5891623509161477 1108 16-17 0.5578468424980016 1108 18-19 0.4269106797189721 1108 20-21 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88-89 2.383565192047957 2315 90-91 2.2108875143455045 2315 92-93 2.289206182925337 2315 94-95 2.7436137824498132 2315 96-97 3.0831543465358244 2315 98-99 3.220474407267101 2315 100-101 3.605601795799025 2316 1 -0.37396624217513974 2316 2 -0.27056252873093456 2316 3 -0.4405810244601831 2316 4 -0.2623865259672229 2316 5 -0.06261691680273884 2316 6 -0.011614017811595545 2316 7 -0.06177201557732559 2316 8 -0.14748129332816262 2316 9 -0.342579975341728 2316 10-11 -0.6914056423365835 2316 12-13 -0.38106787164488054 2316 14-15 -0.45242130806899894 2316 16-17 -0.3111338329866129 2316 18-19 -0.1756531208846397 2316 20-21 -0.3471795021255133 2316 22-23 -0.48124313195463486 2316 24-25 -0.27802127832421775 2316 26-27 -0.3957156306696419 2316 28-29 -0.13145383055827153 2316 30-31 -0.2954904458514136 2316 32-33 -0.34326662850859435 2316 34-35 -0.33037354507546013 2316 36-37 -0.2607988683223894 2316 38-39 -0.17238981142156007 2316 40-41 -0.2981619415039134 2316 42-43 -0.3750428604920941 2316 44-45 -0.3713395763583094 2316 46-47 -0.10355611024130695 2316 48-49 -0.3189279339354911 2316 50-51 -0.5266641768246245 2316 52-53 -0.3761520305857857 2316 54-55 -0.4117121666279644 2316 56-57 -0.4802223480057961 2316 58-59 -0.43564699375246363 2316 60-61 -0.38592641158995633 2316 62-63 -0.4423846335005024 2316 64-65 -0.28281753447600266 2316 66-67 -0.11958322354880835 2316 68-69 -0.1896906986164133 2316 70-71 0.13564830300956388 2316 72-73 -0.029278276338306597 2316 74-75 0.05522549417814915 2316 76-77 -0.130332510289751 2316 78-79 0.010027307008240172 2316 80-81 0.11126531257550099 2316 82-83 0.2882675677381634 2316 84-85 0.44341398094639217 2316 86-87 0.9226562579554525 2316 88-89 0.9793921901167124 2316 90-91 1.3241855793916741 2316 92-93 1.214526607057632 2316 94-95 1.321526839323134 2316 96-97 1.3277086339291024 2316 98-99 1.8695154883080924 2316 100-101 2.182385915656049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9581.0 3 2067.0 4 1894.0 5 2168.0 6 2406.0 7 2902.0 8 3598.0 9 4464.0 10 5471.0 11 6947.0 12 8519.0 13 10575.0 14 11897.0 15 14296.0 16 16658.0 17 20635.0 18 19155.0 19 16225.0 20 16864.0 21 18309.0 22 20582.0 23 23072.0 24 26140.0 25 30002.0 26 34759.0 27 40594.0 28 47713.0 29 56539.0 30 68826.0 31 84056.0 32 104547.0 33 133212.0 34 174518.0 35 233869.0 36 318524.0 37 387367.0 38 249636.0 39 22497.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.397271061789745 18.39799487672652 14.310645335580649 23.89408872590309 2 15.174792168527167 19.342820630418423 40.95235126747143 24.530035933582983 3 19.124363532735625 23.207505582655585 29.78455290888082 27.88357797572797 4 12.538938867980987 15.731169083295782 37.152088227593566 34.57780382112966 5 13.695840739804005 36.74850297268126 35.10106047598522 14.454595811529513 6 33.08417680186647 35.36669457825238 17.479953293722918 14.06917532615823 7 29.035935724893424 31.739706830298474 20.87609911928004 18.348258325528057 8 26.204511682527983 35.17450116767408 19.95449498875062 18.66649216104732 9 25.83412061057691 16.360882545952208 20.28130061465502 37.523696228815865 10-11 24.74759539125469 26.1842496318369 28.066328353623582 21.001826623284828 12-13 25.290884045298395 23.23276867650542 28.496062480299045 22.98028479789714 14-15 22.407418990294882 24.89641250604608 26.241475108172835 26.4546933954862 16-17 22.14301119952208 28.05460582209549 27.01800955799982 22.784373420382607 18-19 22.140818689710994 26.860784380492262 29.059569981926238 21.938826947870506 20-21 22.67983529935455 26.131560204762966 29.17102158913866 22.017582906743822 22-23 22.362795198613703 25.88767401887197 29.121151698853293 22.628379083661038 24-25 22.183861895702446 26.04322925893844 29.317361767059836 22.455547078299283 26-27 22.077805597284815 26.349368497190227 29.052289545429254 22.5205363600957 28-29 22.10751587253009 26.519483843684093 29.04464340364915 22.328356880136663 30-31 22.231042138671942 26.2449475308958 29.023822233223623 22.500188097208635 32-33 22.0765125842334 26.146692708768065 29.071554568775078 22.705240138223463 34-35 22.307540438170793 26.28755434065265 28.972429415301143 22.432475805875413 36-37 22.29521733275911 26.28727608198985 28.922934479116936 22.494572106134104 38-39 22.094260739053976 26.486914264273153 28.97139916935322 22.447425827319652 40-41 22.359491800396572 26.290850582284225 28.860667941097272 22.48898967622193 42-43 22.086718394240318 26.232498988432297 28.89342282906887 22.787359788258517 44-45 22.22968526366087 26.406636795212734 28.793704694572064 22.569973246554333 46-47 22.36793490115428 26.552685817051945 28.450652813575317 22.628726468218463 48-49 22.391370831573095 26.534256655482753 28.43257666442157 22.641795848522577 50-51 22.40840577329281 26.71961010974011 28.344664841782937 22.52731927518414 52-53 22.4157360150703 26.776586181610295 28.111443998170976 22.696233805148424 54-55 22.406234474800875 26.680082516155114 28.126919782687278 22.786763226356737 56-57 22.382025372514853 26.85006715337564 28.140969790439186 22.626937683670324 58-59 22.51240335177007 26.589403715609137 28.263263714340713 22.634929218280075 60-61 22.3603396742789 26.63646667181473 28.398840216939696 22.604353436966672 62-63 22.19340498129278 26.590736921191098 28.615060314430867 22.600797783085252 64-65 22.28147062842229 26.596669380096305 28.472518624972125 22.649341366509276 66-67 22.185295465975905 26.761846901579588 28.206085125890716 22.846772506553794 68-69 22.17664845026303 26.604420673188727 28.40926666531659 22.80966421123165 70-71 22.294061302681992 26.428992365651126 28.358387991568033 22.918558340098848 72-73 22.120494905288552 26.530377432603707 28.289986279831353 23.05914138227639 74-75 22.192912235395024 26.822688377866932 28.018166405502885 22.966232981235155 76-77 22.38446575147339 26.596669515517334 28.07495593184125 22.94390880116803 78-79 22.25907949842945 26.575097701828643 28.155331162404153 23.010491637337758 80-81 22.27520079693387 26.558074931101768 28.084062635690522 23.082661636273844 82-83 22.29884612371633 26.45990453503112 28.191342100015664 23.049907241236877 84-85 22.319268475480257 26.353561580186955 28.216886730481317 23.11028321385147 86-87 22.311948793175272 26.4394798494629 28.248359508796167 23.00021184856566 88-89 22.27921078786352 26.319763640498905 28.07162978297067 23.329395788666904 90-91 22.25054684069728 26.18877904475737 28.37458404166516 23.186090072880194 92-93 22.218495713538257 26.057516769845456 28.471693506802186 23.252294009814094 94-95 22.1108571657472 26.164418619659823 28.367697556264226 23.357026658328753 96-97 22.197926438301135 26.136313813989254 28.222205159534276 23.443554588175335 98-99 22.10930296375775 26.18024958264535 28.193745144030473 23.51670230956643 100-101 22.611123078812952 25.213660567886244 28.812019647603606 23.363196705697202 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2975.0 1 1658.5 2 321.5 3 287.0 4 246.5 5 230.5 6 341.0 7 619.0 8 993.0 9 1234.5 10 1263.5 11 1273.5 12 1347.0 13 1457.0 14 1598.5 15 1775.5 16 1947.0 17 2110.0 18 2324.5 19 2604.0 20 2961.0 21 3394.5 22 4029.0 23 4976.5 24 6203.0 25 7899.5 26 10516.5 27 13843.0 28 16865.5 29 20258.5 30 24229.0 31 28616.0 32 33169.5 33 37027.0 34 42002.0 35 47580.5 36 53355.0 37 58908.5 38 62541.0 39 65526.0 40 68709.0 41 72247.0 42 75366.0 43 78974.5 44 82890.5 45 85704.0 46 87827.5 47 90156.0 48 90819.0 49 90351.0 50 88796.0 51 85169.0 52 82749.0 53 80743.5 54 78359.5 55 72535.5 56 63557.0 57 56197.0 58 50955.5 59 44555.0 60 36738.5 61 29857.0 62 23444.0 63 17663.5 64 12803.0 65 9211.0 66 6708.0 67 5024.0 68 3753.5 69 2869.0 70 2239.5 71 1540.0 72 1099.5 73 820.0 74 552.0 75 351.0 76 234.0 77 155.5 78 93.5 79 57.5 80 41.5 81 33.5 82 26.5 83 16.5 84 13.0 85 10.5 86 6.0 87 5.5 88 6.0 89 6.0 90 7.0 91 6.0 92 3.0 93 3.5 94 3.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26813736487071793 2 0.23433144461448588 3 0.2349977899546219 4 0.2335318302063227 5 0.23548644320405496 6 0.20905474471199442 7 0.2106095505056451 8 0.21052070446029358 9 0.21976069317684582 10-11 0.22822327899657277 12-13 0.22215953640133534 14-15 0.21303060524147244 16-17 0.20854387995122353 18-19 0.1980600465997508 20-21 0.20367955896823087 22-23 0.20103638911902483 24-25 0.202169176197256 26-27 0.1981266811337644 28-29 0.20756657345235743 30-31 0.2179393492471408 32-33 0.20978772458614403 34-35 0.20983214760881974 36-37 0.20379061652492023 38-39 0.19555014581857194 40-41 0.19235168818591925 42-43 0.20310205967344636 44-45 0.1980600465997508 46-47 0.20678917055553214 48-49 0.20896589866664297 50-51 0.21831694493988454 52-53 0.2255801091473667 54-55 0.2104318584149421 56-57 0.19379543642288052 58-59 0.18713198302152073 60-61 0.19037486367684917 62-63 0.19363995584351545 64-65 0.18817592405440045 66-67 0.19079688239226864 68-69 0.19104120901698515 70-71 0.19532803070519328 72-73 0.19512812710315247 74-75 0.19592774151131567 76-77 0.20279109851471622 78-79 0.20352407838886583 80-81 0.19877081496256252 82-83 0.19532803070519328 84-85 0.18484419735372054 86-87 0.1864434261700469 88-89 0.19270707236732507 90-91 0.1917075543571211 92-93 0.19628312569272152 94-95 0.1919074579591619 96-97 0.19845985380383238 98-99 0.19965927541607714 100-101 0.19088572843762008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2251085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.54826133535762 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17830448948077 44.41477236180443 2 12.76337067250011 14.690195819621508 3 4.216807298629486 7.280097852671193 4 1.8758530323360758 4.318083221263981 5 1.0595830127417578 3.0488580061884116 6 0.6318637035329273 2.1817594523543895 7 0.4176961044309177 1.6826379203585897 8 0.3073982167762567 1.4152186330450347 9 0.23234407862652287 1.2033897980869814 >10 1.2041600641602481 12.500883342803812 >50 0.07873953514410781 3.0599111053050247 >100 0.033107172142122464 3.3366766725041046 >500 3.863097493179071E-4 0.1370346849841225 >1k 3.0904779945429097E-4 0.4939829169933309 >5k 7.726194986357274E-5 0.23649821201507898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5319 0.23628605761221813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3971 0.17640382304533148 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3318 0.14739558923807852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2697 0.119808892156449 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.442302267573192E-5 2 0.0 0.0 0.0 0.0 4.442302267573192E-5 3 0.0 0.0 0.0 0.0 4.442302267573192E-5 4 0.0 0.0 0.0 4.442302267573192E-5 4.442302267573192E-5 5 0.0 0.0 0.0 4.442302267573192E-5 4.442302267573192E-5 6 8.884604535146385E-5 0.0 0.0 4.442302267573192E-5 4.442302267573192E-5 7 1.776920907029277E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 8 1.776920907029277E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 9 1.776920907029277E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 10-11 1.776920907029277E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 12-13 2.443266247165256E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 14-15 2.665381360543915E-4 0.0 0.0 4.442302267573192E-5 8.884604535146385E-5 16-17 2.8874964739225746E-4 0.0 0.0 8.884604535146385E-5 8.884604535146385E-5 18-19 3.3317267006798944E-4 0.0 0.0 1.9990360204079366E-4 8.884604535146385E-5 20-21 3.553841814058554E-4 0.0 0.0 3.1096115873012345E-4 8.884604535146385E-5 22-23 3.553841814058554E-4 0.0 0.0 3.998072040815873E-4 1.110575566893298E-4 24-25 5.77499294784515E-4 0.0 0.0 6.441338287981129E-4 1.3326906802719576E-4 26-27 5.77499294784515E-4 2.221151133786596E-5 0.0 8.884604535146386E-4 1.776920907029277E-4 28-29 6.885568514738449E-4 6.663453401359788E-5 0.0 0.0019990360204079367 1.776920907029277E-4 30-31 7.107683628117108E-4 8.884604535146385E-5 0.0 0.004375667733559595 1.9990360204079366E-4 32-33 7.107683628117108E-4 8.884604535146385E-5 0.0 0.007396433275509366 2.2211511337865962E-4 34-35 7.329798741495768E-4 8.884604535146385E-5 0.0 0.010461621840134869 2.2211511337865962E-4 36-37 7.551913854874427E-4 1.776920907029277E-4 0.0 0.014615174460315804 2.2211511337865962E-4 38-39 8.440374308389066E-4 1.776920907029277E-4 0.0 0.020612282521539613 2.665381360543915E-4 40-41 8.440374308389066E-4 1.9990360204079366E-4 0.0 0.03436120803967864 2.665381360543915E-4 42-43 8.440374308389066E-4 2.2211511337865962E-4 0.0 0.04748821124035743 2.665381360543915E-4 44-45 8.884604535146385E-4 2.2211511337865962E-4 0.0 0.062258866280038294 2.8874964739225746E-4 46-47 0.001132787078231164 2.2211511337865962E-4 0.0 0.09322171308502344 3.553841814058554E-4 48-49 0.001199421612244762 2.2211511337865962E-4 0.0 0.11603293522901179 4.886532494330512E-4 50-51 0.001199421612244762 3.1096115873012345E-4 0.0 0.1449301114795754 4.886532494330512E-4 52-53 0.001199421612244762 3.998072040815873E-4 0.0 0.17995766485939002 5.108647607709172E-4 54-55 0.0012882676575962258 3.998072040815873E-4 0.0 0.227357030054396 5.33076272108783E-4 56-57 0.0013771137029476898 3.998072040815873E-4 0.0 0.33601574351923624 5.33076272108783E-4 58-59 0.0013771137029476898 3.998072040815873E-4 0.0 0.43685600499314775 5.33076272108783E-4 60-61 0.0014215367256234215 4.220187154194533E-4 0.0 0.5157512932652477 5.77499294784515E-4 62-63 0.0014215367256234215 4.4423022675731924E-4 0.0 0.609150698440974 5.77499294784515E-4 64-65 0.0014659597482991535 4.4423022675731924E-4 0.0 0.7087249037686272 5.77499294784515E-4 66-67 0.0015103827709748855 4.4423022675731924E-4 0.0 0.863339234191512 5.77499294784515E-4 68-69 0.0015548057936506173 4.4423022675731924E-4 0.0 1.0526479453241437 5.77499294784515E-4 70-71 0.0015770173049884833 4.4423022675731924E-4 0.0 1.1564423378059914 5.77499294784515E-4 72-73 0.0016214403276642152 4.4423022675731924E-4 0.0 1.287090447495319 6.663453401359789E-4 74-75 0.0016658633503399472 4.4423022675731924E-4 0.0 1.5167130517061773 6.663453401359789E-4 76-77 0.0016880748616778132 4.4423022675731924E-4 0.0 1.787671278516804 6.885568514738449E-4 78-79 0.0016880748616778132 4.4423022675731924E-4 0.0 2.0599399844963653 7.107683628117108E-4 80-81 0.0019101899750564727 4.4423022675731924E-4 0.0 2.3004684407741154 7.107683628117108E-4 82-83 0.0019101899750564727 4.4423022675731924E-4 0.0 2.6310201525042367 7.551913854874427E-4 84-85 0.0019546129977322047 4.4423022675731924E-4 0.0 3.1126767758658604 7.551913854874427E-4 86-87 0.0019990360204079367 4.4423022675731924E-4 0.0 3.5047765855132083 7.551913854874427E-4 88-89 0.0019990360204079367 4.4423022675731924E-4 0.0 4.020572301801131 7.551913854874427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3120 0.0 51.33906 1 GTATCAA 5490 0.0 41.297016 1 GTACGGT 55 0.0025782185 34.550724 6 ATCAACG 6845 0.0 32.83116 3 TCAACGC 6940 0.0 32.175644 4 TATCAAC 7080 0.0 31.741426 2 CAACGCA 7015 0.0 31.697601 5 AACGCAG 7150 0.0 31.095654 6 GTACATG 6485 0.0 26.312197 1 ACGCAGA 8435 0.0 26.077469 7 TACATGG 6270 0.0 25.688025 2 CGCAGAG 8460 0.0 25.66347 8 ACATGGG 6540 0.0 23.683092 3 GCAGAGT 9635 0.0 22.188622 9 AGAGTAC 8435 0.0 20.612549 10-11 GAGTACT 5750 0.0 20.446495 12-13 CAGAGTA 8920 0.0 19.917849 10-11 CATGGGG 4245 0.0 19.586157 4 GTACTTT 6050 0.0 19.117706 14-15 AGTACTT 6075 0.0 18.961689 12-13 >>END_MODULE