FastQCFastQC Report
Thu 26 May 2016
SRR937395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251085
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.2345091367051888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45450.2019026380612016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27900.12394023326529208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.046.5418621
GTATCAA61450.038.7381741
ATCAACG76200.031.0422343
TCAACGC77800.030.2817274
TATCAAC78500.030.01172
CAACGCA79800.029.5227895
AACGCAG82050.028.7132036
TATAGCG1004.956359E-528.4990145
TACATGG68700.024.4751362
GTACATG71100.024.0578171
ACGCAGA96900.024.0198447
CGCAGAG96650.023.7870948
ACATGGG69200.023.2687033
CATGGGG42400.021.8447654
GCAGAGT110450.020.4710039
GAGTACT61300.020.2632512-13
GTACTTT63550.019.2086214-15
AGAGTAC95800.019.06464610-11
CCTACAC9050.018.8943753
AGTACTT65000.018.8905712-13
Produced by FastQC (version 0.11.5)