##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2251085 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.283642776705456 33.0 31.0 34.0 31.0 34.0 2 32.492405217928244 34.0 31.0 34.0 31.0 34.0 3 32.56827618681658 34.0 31.0 34.0 31.0 34.0 4 36.16961909479207 37.0 35.0 37.0 35.0 37.0 5 36.08982823838282 37.0 35.0 37.0 35.0 37.0 6 36.122322791009665 37.0 35.0 37.0 35.0 37.0 7 36.06364841842934 37.0 35.0 37.0 35.0 37.0 8 36.03307338461231 37.0 35.0 37.0 35.0 37.0 9 37.75606207673189 39.0 37.0 39.0 35.0 39.0 10-11 37.66952469586888 39.0 37.0 39.0 35.0 39.0 12-13 37.56446691262214 39.0 37.0 39.0 35.0 39.0 14-15 38.84882778748914 40.0 38.0 41.0 35.0 41.0 16-17 38.79739570029564 40.0 38.0 41.0 35.0 41.0 18-19 38.74319650301966 40.0 38.0 41.0 35.0 41.0 20-21 38.612942869771686 40.0 38.0 41.0 34.0 41.0 22-23 38.55573978770238 40.0 38.0 41.0 34.0 41.0 24-25 38.59543664499564 40.0 38.0 41.0 34.0 41.0 26-27 38.45490441276095 40.0 38.0 41.0 34.0 41.0 28-29 38.31830539495399 40.0 38.0 41.0 34.0 41.0 30-31 38.414861278005944 40.0 38.0 41.0 34.0 41.0 32-33 38.355835297201125 40.0 38.0 41.0 34.0 41.0 34-35 38.24578614312654 40.0 38.0 41.0 34.0 41.0 36-37 38.10806455553655 40.0 38.0 41.0 33.5 41.0 38-39 38.00440187731694 40.0 38.0 41.0 33.0 41.0 40-41 37.845524713638085 40.0 37.0 41.0 33.0 41.0 42-43 37.67778271366919 40.0 37.0 41.0 33.0 41.0 44-45 37.43733399671714 40.0 37.0 41.0 32.5 41.0 46-47 37.172511033568256 40.0 37.0 41.0 31.5 41.0 48-49 36.91695404660419 39.5 36.0 41.0 31.0 41.0 50-51 36.70059615696431 39.0 36.0 41.0 31.0 41.0 52-53 36.46656279083197 39.0 36.0 40.5 30.5 41.0 54-55 36.25637903499868 39.0 35.0 40.0 30.0 41.0 56-57 35.95045189319817 38.5 35.0 40.0 29.0 41.0 58-59 35.68627683983502 38.0 35.0 40.0 29.0 41.0 60-61 35.33583405335649 38.0 34.0 40.0 28.0 41.0 62-63 34.95998174213768 37.5 34.0 40.0 28.0 41.0 64-65 34.52945424095492 37.0 34.0 39.5 27.0 41.0 66-67 34.085141609490535 36.0 33.0 39.0 26.0 40.5 68-69 33.62290051241956 36.0 33.0 39.0 26.0 40.0 70-71 33.180115144474776 35.0 32.0 38.5 25.5 40.0 72-73 32.6927266184973 35.0 32.0 37.5 24.5 39.5 74-75 32.20461532994089 35.0 31.0 37.0 23.5 39.0 76-77 30.893253253431123 33.5 29.5 35.5 22.5 38.0 78-79 31.543420395053943 34.0 31.0 36.0 23.0 38.0 80-81 31.364878047696997 34.0 31.0 36.0 22.5 37.0 82-83 30.96721447657463 34.0 31.0 35.0 20.5 37.0 84-85 30.54722700386702 34.0 30.5 35.0 19.5 36.5 86-87 30.16349760226735 34.0 30.0 35.0 18.0 36.0 88-89 29.7197200461111 34.0 30.0 35.0 14.5 36.0 90-91 29.176601505496237 34.0 29.0 35.0 7.0 35.0 92-93 28.66329103521191 33.5 29.0 35.0 2.0 35.0 94-95 28.16997958762108 33.0 28.5 35.0 2.0 35.0 96-97 27.48734499141525 33.0 27.0 35.0 2.0 35.0 98-99 26.490023477567483 32.5 25.5 34.0 2.0 35.0 100-101 23.481336111253018 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1478877076615177 1101 2 0.18871664605253358 1101 3 0.1462178025986276 1101 4 0.08712102962108048 1101 5 0.08051280831791985 1101 6 0.14218728141737813 1101 7 0.13046443219847248 1101 8 0.03738763894196495 1101 9 0.1433058071310498 1101 10-11 0.16854034398901518 1101 12-13 0.20148232092254403 1101 14-15 0.3143476614331391 1101 16-17 0.133886945441283 1101 18-19 0.14475875555275053 1101 20-21 0.23494612827506245 1101 22-23 0.14648069286933207 1101 24-25 0.11417137446346715 1101 26-27 0.11056941156149236 1101 28-29 -0.034695226751559005 1101 30-31 0.19790975238487363 1101 32-33 0.1815984464723499 1101 34-35 0.15024020571683394 1101 36-37 0.22916688003608243 1101 38-39 0.1727668582731141 1101 40-41 0.18836061041189822 1101 42-43 0.2624550219712134 1101 44-45 0.2840885728730811 1101 46-47 0.3050909294393378 1101 48-49 0.25775611369142126 1101 50-51 0.33078597709039315 1101 52-53 0.26092922081987524 1101 54-55 0.1889295306934713 1101 56-57 0.3964658787872821 1101 58-59 0.3421216484715188 1101 60-61 0.4406313540862925 1101 62-63 0.4961953627754596 1101 64-65 0.5164778669009422 1101 66-67 0.35762222648971687 1101 68-69 0.46161596188392195 1101 70-71 0.38909904627310254 1101 72-73 0.4005061058540136 1101 74-75 0.4436674643723606 1101 76-77 0.41839661058067534 1101 78-79 0.44503613532793906 1101 80-81 0.35400226186423467 1101 82-83 0.30475284692103344 1101 84-85 0.45059329058133457 1101 86-87 0.7073477899189733 1101 88-89 0.9127949285472212 1101 90-91 0.9074467382319078 1101 92-93 0.8881882834301855 1101 94-95 0.9727200545989199 1101 96-97 1.1983357026673964 1101 98-99 1.4413229351938632 1101 100-101 2.0598936774234566 1102 1 0.16376811644370548 1102 2 0.20856445031736115 1102 3 0.15500523296161361 1102 4 0.14686856333380405 1102 5 0.06745453264004908 1102 6 0.09830227728231478 1102 7 0.11213208917440909 1102 8 0.07290100354801865 1102 9 0.17903034500824333 1102 10-11 0.0654007258769056 1102 12-13 0.05798680532502232 1102 14-15 0.11404281106320724 1102 16-17 0.21625216951439796 1102 18-19 0.19041668147364277 1102 20-21 0.10603626906584651 1102 22-23 0.14333405220300222 1102 24-25 0.03897233421916013 1102 26-27 0.01928653691915372 1102 28-29 0.06348544499399367 1102 30-31 0.14288600383482475 1102 32-33 0.19568979252017726 1102 34-35 0.21537285650159532 1102 36-37 0.25027228768095 1102 38-39 0.17918921752950467 1102 40-41 0.1848124326107552 1102 42-43 0.21733838896711433 1102 44-45 0.28502112238209065 1102 46-47 0.2916203891039473 1102 48-49 0.32266661536004904 1102 50-51 0.2874413222474459 1102 52-53 0.2608382851991138 1102 54-55 0.2880890888864087 1102 56-57 0.2796230920232148 1102 58-59 0.21918488384071821 1102 60-61 0.3483975540858566 1102 62-63 0.28610929625806136 1102 64-65 0.44628723882237153 1102 66-67 0.3543037468021808 1102 68-69 0.3480184493977774 1102 70-71 0.3179469143070932 1102 72-73 0.5413915555785849 1102 74-75 0.3747029524080929 1102 76-77 0.30480649809862825 1102 78-79 0.29906072439925424 1102 80-81 0.2808609690666337 1102 82-83 0.36199729200118114 1102 84-85 0.5484484118112789 1102 86-87 0.5206432034461024 1102 88-89 0.6725053728434673 1102 90-91 0.9218235059140945 1102 92-93 0.9441031613321051 1102 94-95 0.9131834458350525 1102 96-97 1.0833931773568999 1102 98-99 1.505488867323212 1102 100-101 2.431454041293083 1103 1 0.2279453030691201 1103 2 0.2624979843489399 1103 3 0.2324790629780651 1103 4 0.14441707401614678 1103 5 0.10588481922282256 1103 6 0.09594450134584775 1103 7 0.14034278322252192 1103 8 0.07849732936823983 1103 9 0.14637139645699904 1103 10-11 0.2233568271453592 1103 12-13 0.17399254546188558 1103 14-15 0.1902743619965932 1103 16-17 0.25068431146831216 1103 18-19 0.30099550497410377 1103 20-21 0.2116784957661011 1103 22-23 0.22302206684382497 1103 24-25 0.16670850677131455 1103 26-27 0.12277049940655616 1103 28-29 0.13946315680986032 1103 30-31 0.15102105042015523 1103 32-33 0.17861555504278925 1103 34-35 0.23749415831120047 1103 36-37 0.1900778648246444 1103 38-39 0.1561995013920594 1103 40-41 0.04852730355106161 1103 42-43 0.17424160446767445 1103 44-45 0.07024768574211038 1103 46-47 0.10877871593886823 1103 48-49 0.1112627955015526 1103 50-51 0.009247931666557463 1103 52-53 0.09646056512153223 1103 54-55 0.13243665588708353 1103 56-57 0.11835137177732236 1103 58-59 0.12335603018278363 1103 60-61 0.1478391080273056 1103 62-63 0.20567283799545777 1103 64-65 0.1705347357522342 1103 66-67 0.33287798944315483 1103 68-69 0.31700448379685753 1103 70-71 0.2968475058329858 1103 72-73 0.22134563712042876 1103 74-75 0.30132147478647653 1103 76-77 0.3129935649488971 1103 78-79 0.3556482807124297 1103 80-81 0.3904856848777847 1103 82-83 0.4222772983556773 1103 84-85 0.6293737616873862 1103 86-87 0.7162760939644848 1103 88-89 0.812046245390853 1103 90-91 1.0135250706529 1103 92-93 1.008947053296069 1103 94-95 0.8868132364510153 1103 96-97 1.0945496839769326 1103 98-99 1.4326434126638965 1103 100-101 2.2062124464385526 1104 1 0.090871520425722 1104 2 0.13604077990072483 1104 3 0.14100357174756795 1104 4 0.1022022469911903 1104 5 0.09220794029481993 1104 6 0.0714996622827968 1104 7 0.11008099836702456 1104 8 0.08237952924880432 1104 9 0.09713079760431498 1104 10-11 0.09650940732121427 1104 12-13 0.1425024823262575 1104 14-15 0.14210781689252627 1104 16-17 0.1369018887842941 1104 18-19 0.12596364843959407 1104 20-21 0.1761848931022243 1104 22-23 0.10614790770752336 1104 24-25 0.00693651829373465 1104 26-27 -0.029303973819409634 1104 28-29 -0.10300511791648148 1104 30-31 0.01154650404482993 1104 32-33 0.09908558147442648 1104 34-35 0.1168649355994944 1104 36-37 0.09935136862652882 1104 38-39 0.038088201778101904 1104 40-41 0.15188432626292325 1104 42-43 0.14453172828985572 1104 44-45 0.085933942547193 1104 46-47 0.1336096458330971 1104 48-49 0.2111708070061411 1104 50-51 0.16233420031019108 1104 52-53 0.21948074183709565 1104 54-55 0.2956332984630592 1104 56-57 0.25079763815580236 1104 58-59 0.19603948240891356 1104 60-61 0.22689208356863588 1104 62-63 0.23016527653444285 1104 64-65 0.06238811331406424 1104 66-67 0.1115131708958117 1104 68-69 0.20212543384226223 1104 70-71 0.15133345545980603 1104 72-73 0.24178727107778286 1104 74-75 0.2661717142385953 1104 76-77 0.3161228124636075 1104 78-79 0.2146773606480039 1104 80-81 0.30770172418447217 1104 82-83 0.20048848775510564 1104 84-85 0.1782910296736624 1104 86-87 0.16972492123936433 1104 88-89 0.2606721421588496 1104 90-91 0.44761846840493646 1104 92-93 0.4611009407296578 1104 94-95 0.6307831798901269 1104 96-97 0.7870058470333383 1104 98-99 0.9193501163979612 1104 100-101 1.5799886131914427 1105 1 0.04480731637998758 1105 2 0.0148957179096314 1105 3 0.03782823165845173 1105 4 -0.02577608514281593 1105 5 -0.035159121247488656 1105 6 -0.027709834162635616 1105 7 -0.1288908763078851 1105 8 0.01731134377675403 1105 9 0.10550589249741193 1105 10-11 0.0767005540424961 1105 12-13 0.03604709734184297 1105 14-15 -0.006909378639271324 1105 16-17 0.052871687632425335 1105 18-19 0.13241960299750843 1105 20-21 0.08493147888516006 1105 22-23 0.14625335188458877 1105 24-25 0.02282186980870904 1105 26-27 0.016525684152767894 1105 28-29 0.022334154456579824 1105 30-31 0.014344990655771994 1105 32-33 0.10104582782249594 1105 34-35 0.06980113004028254 1105 36-37 0.2414592075915465 1105 38-39 0.03221106286836317 1105 40-41 0.038204975946406705 1105 42-43 -0.04178194680478242 1105 44-45 -0.05658237734592575 1105 46-47 0.0063325876523379065 1105 48-49 0.014400621965677374 1105 50-51 0.034541087034277496 1105 52-53 0.02102623120175906 1105 54-55 0.064760597928057 1105 56-57 0.2397619364283372 1105 58-59 0.1403610386814833 1105 60-61 0.19427615494028316 1105 62-63 0.3045953698757202 1105 64-65 0.352683558617386 1105 66-67 0.28110900368105973 1105 68-69 0.21576145358518772 1105 70-71 0.25563300470147965 1105 72-73 0.22699464097214417 1105 74-75 0.21927012966359172 1105 76-77 0.2559926681401272 1105 78-79 0.18784518236676462 1105 80-81 0.23920669430443198 1105 82-83 0.22898290722428882 1105 84-85 0.14877041068266195 1105 86-87 0.3205963472193112 1105 88-89 0.39558051819945206 1105 90-91 0.6712702126754557 1105 92-93 0.6162566879198366 1105 94-95 0.690200551323418 1105 96-97 0.9752596184697246 1105 98-99 1.0816908185080045 1105 100-101 1.5711485098858553 1106 1 -0.07799092467981694 1106 2 0.05501404212830607 1106 3 -0.03079073228697382 1106 4 0.0527231809375408 1106 5 -0.0035488544461088622 1106 6 0.011115720594276013 1106 7 0.04217561714850859 1106 8 -0.0439032455814683 1106 9 0.0028474101468063395 1106 10-11 -0.17463699909519192 1106 12-13 -0.33829572499129057 1106 14-15 -0.058719396053739104 1106 16-17 -0.039528109067909156 1106 18-19 0.0496069470606173 1106 20-21 0.21614386480241876 1106 22-23 0.15255088986170762 1106 24-25 0.16080794439827173 1106 26-27 0.20163932395688988 1106 28-29 0.18174385654911873 1106 30-31 0.0742872049961818 1106 32-33 0.1801209741298493 1106 34-35 -0.24768268335910903 1106 36-37 0.23935794910718045 1106 38-39 0.31680368420058613 1106 40-41 0.10835317276919199 1106 42-43 0.10262326150169088 1106 44-45 0.16662002412438426 1106 46-47 0.24149040840998737 1106 48-49 0.27072381068674645 1106 50-51 0.1967456689579805 1106 52-53 0.24941632042710182 1106 54-55 0.23799766294469293 1106 56-57 0.21645921672060808 1106 58-59 0.34199459860105463 1106 60-61 0.2258719844376813 1106 62-63 0.2098023447475228 1106 64-65 0.1590429387159915 1106 66-67 0.1355610056725709 1106 68-69 0.24477482785504634 1106 70-71 0.3582762087401079 1106 72-73 0.445282542118953 1106 74-75 0.4003280784787222 1106 76-77 0.3082805883497777 1106 78-79 0.38962024188209554 1106 80-81 0.37705330173423235 1106 82-83 0.33708095922549575 1106 84-85 0.6168207270698751 1106 86-87 0.6756528237097719 1106 88-89 0.6675215057895372 1106 90-91 0.8244146855148209 1106 92-93 0.8073176559536819 1106 94-95 0.5609629923547388 1106 96-97 0.9284913739225367 1106 98-99 1.1997153580000592 1106 100-101 1.9881554577332672 1107 1 0.027866289802801703 1107 2 0.03553152277189042 1107 3 0.05723981480954876 1107 4 0.08089070071530813 1107 5 0.06856520397204235 1107 6 0.023490580623629853 1107 7 0.09401204413369868 1107 8 0.00876860987607131 1107 9 0.034606423589444546 1107 10-11 0.008252116266078247 1107 12-13 0.008473173546583723 1107 14-15 0.06384282608899383 1107 16-17 6.430112974484814E-4 1107 18-19 0.06589242169977894 1107 20-21 0.08892737242585014 1107 22-23 0.09706264618334615 1107 24-25 -0.05936777446125063 1107 26-27 0.016564029298606897 1107 28-29 0.0752546901406177 1107 30-31 0.04726794467170237 1107 32-33 -0.013422430865624335 1107 34-35 -0.06681886062514053 1107 36-37 -0.035289236959961556 1107 38-39 -0.10021001670510543 1107 40-41 -0.014485959355212685 1107 42-43 0.0034175702816341413 1107 44-45 -0.07509579014858758 1107 46-47 -0.008307024435936228 1107 48-49 0.06497128242434513 1107 50-51 0.09546823031620022 1107 52-53 0.005035834896446545 1107 54-55 -0.0068072056996300034 1107 56-57 -0.03337618325744529 1107 58-59 0.054398043864431145 1107 60-61 0.13123485321903416 1107 62-63 0.13246306924224172 1107 64-65 0.2574673860934027 1107 66-67 0.3038914309711416 1107 68-69 0.30311310727253016 1107 70-71 0.33249455567087693 1107 72-73 0.315816819239906 1107 74-75 0.2188697404721225 1107 76-77 0.1638271695497231 1107 78-79 0.25001183836893404 1107 80-81 0.2737166198160459 1107 82-83 0.38616624689359114 1107 84-85 0.3221325789383407 1107 86-87 0.5016414241730764 1107 88-89 0.5894175828526613 1107 90-91 0.4876308395974185 1107 92-93 0.4554482028683253 1107 94-95 0.4750372339366429 1107 96-97 0.7898843629922467 1107 98-99 0.9218658982094468 1107 100-101 1.603484524399608 1108 1 0.14979699702883664 1108 2 0.1719327684957932 1108 3 0.16302848346890642 1108 4 0.05330775615562544 1108 5 0.06712115812188557 1108 6 0.12643318716656893 1108 7 0.048627304498729984 1108 8 0.0964058550099125 1108 9 0.12491069305346514 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2316 84-85 0.10445656845017126 2316 86-87 0.22990274152751056 2316 88-89 0.2810492593284266 2316 90-91 0.4097569319133605 2316 92-93 0.3694399845489187 2316 94-95 0.906730834568652 2316 96-97 1.2387355430731226 2316 98-99 1.6671656479602497 2316 100-101 2.1894042911783416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 4.0 4 7.0 5 49.0 6 71.0 7 198.0 8 443.0 9 828.0 10 1365.0 11 2355.0 12 3601.0 13 4848.0 14 6295.0 15 8340.0 16 11160.0 17 15536.0 18 14313.0 19 10056.0 20 9570.0 21 10215.0 22 11066.0 23 12247.0 24 14406.0 25 17014.0 26 21036.0 27 26423.0 28 32826.0 29 41438.0 30 52445.0 31 66263.0 32 86487.0 33 113300.0 34 159294.0 35 229751.0 36 354617.0 37 518570.0 38 368345.0 39 26296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78004114503915 19.379811198579254 14.463205677642085 23.376941978739506 2 15.90655172949933 20.373997427906986 39.40299899826084 24.316451844332843 3 19.5767818629683 23.74104043161409 29.555925253822046 27.126252451595562 4 13.270933794148155 16.535492884542343 36.32852602189611 33.86504729941339 5 13.87665149313504 37.490543381833255 34.40898524212274 14.223819882908964 6 33.41379823507331 35.987357207746484 16.73441918008427 13.864425377095934 7 29.355399729463795 32.335829166824 20.211009357709727 18.097761746002483 8 26.59943982568406 35.9152142189211 19.1579616051815 18.32738435021334 9 26.27395233853897 16.55957016283259 19.68775057361228 37.47872692501616 10-11 25.061159396468813 26.82610829888698 27.187933818580817 20.924798486063388 12-13 25.585807391420918 23.766017784741674 27.933501690045798 22.71467313379161 14-15 22.629998422982695 25.58053116608213 25.528511806528854 26.26095860440632 16-17 22.439090483033738 28.757288152157738 26.339476296985676 22.464145067822848 18-19 22.424675212175462 27.568150469662406 28.299842076154384 21.707332242007745 20-21 22.982715430750446 26.758742347572255 28.50798926561126 21.750552956066034 22-23 22.655232944649867 26.559592689756673 28.434175802916002 22.350998562677457 24-25 22.500663569638164 26.795308479364927 28.57276443666547 22.13126351433144 26-27 22.37542105678172 26.984883603413103 28.393592718008293 22.246102621796886 28-29 22.34825279642754 27.229489866046023 28.34055309713873 22.081704240387705 30-31 22.56041260149284 26.983706727763195 28.287212929779336 22.168667740964626 32-33 22.534420099125622 26.827287132460242 28.329230863544346 22.30906190486979 34-35 22.64170388945775 26.984654066816667 28.342887985127174 22.030754058598408 36-37 22.833056059633467 26.947649688927783 28.19460393543558 22.024690316003174 38-39 22.476139283945297 27.179959886010526 28.242114358187276 22.101786471856904 40-41 22.719488602162958 27.02179171377358 28.132811510893635 22.12590817316983 42-43 22.49972011046307 27.037040492463422 28.106351804265696 22.356887592807816 44-45 22.608142295826237 27.251258837405075 27.999386962287076 22.141211904481615 46-47 22.78605650164254 27.404140669943605 27.617415601809792 22.192387226604062 48-49 22.795296490359092 27.39456750855698 27.590317557977595 22.219818443106323 50-51 22.79605168174458 27.603555618734966 27.53292301268055 22.067469686839903 52-53 22.91858055941049 27.623136231255696 27.238987972017036 22.21929523731678 54-55 22.837142977719633 27.63807230735401 27.2141434019595 22.310641312966858 56-57 22.756270864938465 27.776516657522926 27.286686197988967 22.18052627954964 58-59 22.784701599450933 27.48621220433702 27.380907429084196 22.348178767127852 60-61 22.71497966536137 27.60164542875991 27.523261005248578 22.16011390063014 62-63 22.55938802843962 27.544406364042228 27.688758976227017 22.207446631291134 64-65 22.62095833786818 27.49791767081208 27.57019392870549 22.31093006261425 66-67 22.528203066521254 27.692468298620444 27.334218832252006 22.4451098026063 68-69 22.518963077804703 27.489055277788268 27.502226704011623 22.48975494039541 70-71 22.616071805373853 27.4737071234538 27.45225080350142 22.457970267670923 72-73 22.519341267449118 27.48775532460967 27.38089552994662 22.61200787799459 74-75 22.446931146536002 27.844128498035392 27.02916593553775 22.67977441989085 76-77 22.605765664113083 27.611240801657864 27.251347683450426 22.531645850778624 78-79 22.637905721018974 27.62525626531206 27.111526219578558 22.625311794090404 80-81 22.683483742284277 27.570238351728165 27.088248555696477 22.65802935029108 82-83 22.46361569269062 27.608250019379643 27.182542018285943 22.745592269643794 84-85 22.5189114486424 27.56243826583954 27.193882412624852 22.72476787289321 86-87 22.522916726822842 27.514976111519555 27.232445687301905 22.7296614743557 88-89 22.458236805806976 27.534033588247446 26.973326196034357 23.03440340991122 90-91 22.493592994488985 27.423360840639997 27.180320024843873 22.90272614002714 92-93 22.250014160042912 27.318398837876952 27.354981725215204 23.076605276864935 94-95 22.193675494261655 27.474506737861965 27.238331737806437 23.093486030069943 96-97 22.305177281177745 27.492942292272392 27.039627557377887 23.162252869171976 98-99 22.014687178903984 27.550410269919446 26.932306684927536 23.502595866249028 100-101 21.933741692387184 27.326809125834096 27.346424399890353 23.39302478188837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 127.5 2 98.0 3 137.5 4 157.0 5 164.0 6 262.0 7 542.0 8 902.5 9 1097.5 10 1146.0 11 1213.0 12 1335.0 13 1500.0 14 1672.0 15 1816.5 16 1965.0 17 2142.5 18 2339.5 19 2588.0 20 2862.5 21 3348.5 22 4130.5 23 5029.5 24 6161.5 25 7803.5 26 10310.5 27 13859.0 28 17080.5 29 20420.5 30 24515.5 31 28470.0 32 33183.5 33 37098.0 34 41477.5 35 47289.0 36 53310.5 37 58960.5 38 62901.0 39 65668.0 40 68561.0 41 72253.0 42 75590.0 43 79517.0 44 83788.5 45 86226.5 46 87716.0 47 90240.5 48 91382.0 49 90615.5 50 88713.5 51 85470.5 52 83191.5 53 81513.5 54 79831.0 55 74120.0 56 63941.0 57 55867.5 58 51185.5 59 45326.5 60 36798.0 61 29138.0 62 22981.0 63 17224.5 64 12039.0 65 8286.5 66 6123.5 67 4865.0 68 3732.0 69 2952.5 70 2351.0 71 1509.0 72 969.0 73 683.5 74 473.5 75 294.0 76 183.0 77 107.5 78 62.5 79 36.0 80 23.0 81 12.0 82 7.0 83 5.0 84 2.0 85 1.5 86 1.0 87 0.5 88 1.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044245330585029 2 0.0 3 0.0 4 0.0 5 7.107683628117109E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004908744005668378 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.884604535146386E-5 22-23 0.0010883640555554323 24-25 1.1105755668932982E-4 26-27 0.002332208690475926 28-29 0.003909225995464409 30-31 1.1105755668932982E-4 32-33 8.884604535146386E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00875133546711919 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.442302267573193E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.9990360204079366E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.108647607709172E-4 84-85 6.66345340135979E-5 86-87 0.0 88-89 0.0 90-91 0.010150660681404744 92-93 0.0014437482369612875 94-95 0.0 96-97 0.0 98-99 2.665381360543916E-4 100-101 0.012793830530610794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2251085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.22420894280952 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49119242988837 41.68941384089995 2 13.468208233327958 14.875422911251409 3 4.569387875322312 7.570224923026195 4 2.0789297498604173 4.592290035348577 5 1.1825311901949427 3.265217476435737 6 0.7280702006711529 2.41242605321382 7 0.45891989142263206 1.7740441580354427 8 0.31815478600758984 1.4055877102910392 9 0.24831717051568233 1.234180737778077 >10 1.3341057452450715 13.372225057492068 >50 0.08233563910668407 3.0965194687462825 >100 0.03831607428371004 3.676677320444431 >500 0.0011281156921348677 0.3755757600702356 >1k 3.223187691812673E-4 0.4252816989324704 >5k 8.057969229531682E-5 0.2349128480343739 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5279 0.2345091367051888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4545 0.2019026380612016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2790 0.12394023326529208 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.442302267573192E-5 0.0 0.0 0.0 4 0.0 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 5 0.0 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 6 0.0 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 7 0.0 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 8 4.442302267573192E-5 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 9 4.442302267573192E-5 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 10-11 8.884604535146385E-5 4.442302267573192E-5 0.0 4.442302267573192E-5 0.0 12-13 1.3326906802719576E-4 4.442302267573192E-5 0.0 4.442302267573192E-5 2.221151133786596E-5 14-15 1.776920907029277E-4 4.442302267573192E-5 0.0 4.442302267573192E-5 4.442302267573192E-5 16-17 1.9990360204079366E-4 4.442302267573192E-5 0.0 8.884604535146385E-5 4.442302267573192E-5 18-19 2.887496473922575E-4 4.442302267573192E-5 0.0 1.9990360204079366E-4 4.442302267573192E-5 20-21 3.553841814058554E-4 4.442302267573192E-5 0.0 2.8874964739225746E-4 4.442302267573192E-5 22-23 3.553841814058554E-4 4.442302267573192E-5 0.0 3.553841814058554E-4 8.884604535146385E-5 24-25 5.33076272108783E-4 4.442302267573192E-5 0.0 5.997108061223809E-4 1.3326906802719576E-4 26-27 5.33076272108783E-4 8.884604535146385E-5 0.0 8.440374308389066E-4 1.3326906802719576E-4 28-29 5.774992947845149E-4 8.884604535146385E-5 0.0 0.0020434590430836687 1.3326906802719576E-4 30-31 6.219223174602469E-4 8.884604535146385E-5 0.0 0.004686628892289718 1.5548057936506173E-4 32-33 6.441338287981129E-4 8.884604535146385E-5 0.0 0.007818451990928819 1.776920907029277E-4 34-35 6.885568514738449E-4 1.3326906802719576E-4 0.0 0.01106133264625725 1.776920907029277E-4 36-37 7.996144081631746E-4 1.3326906802719576E-4 0.0 0.015437000379816844 1.776920907029277E-4 38-39 8.884604535146385E-4 1.3326906802719576E-4 0.0 0.021811704133784374 1.776920907029277E-4 40-41 8.884604535146385E-4 1.776920907029277E-4 0.0 0.03620476348072152 1.776920907029277E-4 42-43 0.0010883640555554323 2.2211511337865962E-4 0.0 0.049975900510198415 1.776920907029277E-4 44-45 0.00115499858956903 2.2211511337865962E-4 0.0 0.06501309368593367 1.776920907029277E-4 46-47 0.0013104791689340918 2.2211511337865962E-4 0.0 0.09688661245577132 1.776920907029277E-4 48-49 0.0013326906802719578 2.2211511337865962E-4 0.0 0.12029754540588206 2.2211511337865962E-4 50-51 0.0013326906802719578 3.1096115873012345E-4 0.0 0.14966116339454086 2.2211511337865962E-4 52-53 0.0013326906802719578 3.998072040815873E-4 0.0 0.18537727362582934 2.665381360543915E-4 54-55 0.0013326906802719578 3.998072040815873E-4 0.0 0.23397606043308006 3.1096115873012345E-4 56-57 0.0013771137029476898 4.4423022675731924E-4 0.0 0.34356765737411066 3.1096115873012345E-4 58-59 0.0013771137029476898 4.4423022675731924E-4 0.0 0.44574060952829414 3.1096115873012345E-4 60-61 0.0013771137029476898 4.4423022675731924E-4 0.0 0.5263017611507339 3.1096115873012345E-4 62-63 0.0013771137029476898 4.886532494330512E-4 0.0 0.620922799450043 3.1096115873012345E-4 64-65 0.0013771137029476898 4.886532494330512E-4 0.0 0.7220962335940224 3.1096115873012345E-4 66-67 0.0014215367256234215 4.886532494330512E-4 0.0 0.8786651770146396 3.1096115873012345E-4 68-69 0.0014215367256234215 4.886532494330512E-4 0.0 1.0699062896336655 3.1096115873012345E-4 70-71 0.0014659597482991535 4.886532494330512E-4 0.0 1.1747668346597306 3.1096115873012345E-4 72-73 0.0014881712596370195 4.886532494330512E-4 0.0 1.307325134324115 3.553841814058554E-4 74-75 0.0015548057936506173 4.886532494330512E-4 0.0 1.5403683112810045 3.553841814058554E-4 76-77 0.0015992288163263492 4.886532494330512E-4 0.0 1.8166350893013812 3.553841814058554E-4 78-79 0.0015992288163263492 4.886532494330512E-4 0.0 2.093723693241259 3.553841814058554E-4 80-81 0.0017102863730156792 4.886532494330512E-4 0.0 2.338672240275245 3.553841814058554E-4 82-83 0.001732497884353545 4.886532494330512E-4 0.0 2.6760428859860914 3.553841814058554E-4 84-85 0.0018213439297050087 4.886532494330512E-4 0.0 3.169249495243405 3.553841814058554E-4 86-87 0.0019768245090700707 4.886532494330512E-4 0.0 3.5691677568816815 3.553841814058554E-4 88-89 0.0020434590430836687 4.886532494330512E-4 0.0 4.09740191951881 3.998072040815873E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3665 0.0 46.541862 1 GTATCAA 6145 0.0 38.738174 1 ATCAACG 7620 0.0 31.042234 3 TCAACGC 7780 0.0 30.281727 4 TATCAAC 7850 0.0 30.0117 2 CAACGCA 7980 0.0 29.522789 5 AACGCAG 8205 0.0 28.713203 6 TATAGCG 100 4.956359E-5 28.499014 5 TACATGG 6870 0.0 24.475136 2 GTACATG 7110 0.0 24.057817 1 ACGCAGA 9690 0.0 24.019844 7 CGCAGAG 9665 0.0 23.787094 8 ACATGGG 6920 0.0 23.268703 3 CATGGGG 4240 0.0 21.844765 4 GCAGAGT 11045 0.0 20.471003 9 GAGTACT 6130 0.0 20.26325 12-13 GTACTTT 6355 0.0 19.20862 14-15 AGAGTAC 9580 0.0 19.064646 10-11 CCTACAC 905 0.0 18.894375 3 AGTACTT 6500 0.0 18.89057 12-13 >>END_MODULE