FastQCFastQC Report
Thu 26 May 2016
SRR937394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276460
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.24252567583001677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.1792695676620718No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28670.12594115424826266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.12321762736880948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.049.073371
GTATCAA57900.042.3751871
ATCAACG73700.032.8919873
TCAACGC73550.032.833434
CAACGCA75400.032.026435
TATCAAC77050.031.4625952
AACGCAG78050.030.9158956
ACGCAGA90750.026.1183017
CGCAGAG90950.025.7997348
GTACCGT2151.6643753E-924.302186
GTACATG69300.024.289051
TACATGG66000.024.2707372
ACATGGG68550.022.396623
GCAGAGT101300.022.3197089
AGAGTAC89300.020.82434310-11
GAGTACT57600.019.87357512-13
TATTCCG2401.4036777E-719.806375
GGACCGT2902.4429028E-919.6550566
CAGAGTA96050.019.28671510-11
AGTACTT62350.019.19753612-13
Produced by FastQC (version 0.11.5)