##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.709603946478303 33.0 31.0 34.0 30.0 34.0 2 31.824625075775547 33.0 31.0 34.0 30.0 34.0 3 31.8695189021551 33.0 31.0 34.0 30.0 34.0 4 35.478424835050916 37.0 35.0 37.0 33.0 37.0 5 35.47629213779289 37.0 35.0 37.0 33.0 37.0 6 35.502553086810224 37.0 35.0 37.0 33.0 37.0 7 35.44225332314207 37.0 35.0 37.0 33.0 37.0 8 35.42735870606116 37.0 35.0 37.0 33.0 37.0 9 37.05512242692602 39.0 37.0 39.0 33.0 39.0 10-11 36.929876430949804 39.0 37.0 39.0 33.0 39.0 12-13 36.793534259332475 39.0 37.0 39.0 33.0 39.0 14-15 37.999477258550556 40.0 38.0 41.0 33.0 41.0 16-17 37.95622874990116 40.0 38.0 41.0 33.0 41.0 18-19 37.897922871475885 40.0 38.0 41.0 33.0 41.0 20-21 37.81329454503923 40.0 38.0 41.0 33.0 41.0 22-23 37.662389411630336 40.0 37.5 41.0 32.0 41.0 24-25 37.51038959612732 40.0 37.0 41.0 32.0 41.0 26-27 37.352893088391625 40.0 37.0 41.0 32.0 41.0 28-29 37.409116566950445 40.0 37.0 41.0 32.0 41.0 30-31 37.27488600722174 40.0 37.0 41.0 31.5 41.0 32-33 37.10097080554897 39.0 36.5 41.0 31.0 41.0 34-35 37.00756613338253 39.0 36.5 41.0 31.0 41.0 36-37 37.05614067455611 40.0 37.0 41.0 31.0 41.0 38-39 36.98535181817383 40.0 37.0 41.0 31.0 41.0 40-41 36.83194982560643 40.0 36.0 41.0 30.5 41.0 42-43 36.62369358565492 39.0 36.0 41.0 30.0 41.0 44-45 36.44163745464449 39.0 36.0 41.0 30.0 41.0 46-47 36.162879207190116 39.0 35.5 41.0 29.5 41.0 48-49 35.89982231183504 39.0 35.0 41.0 28.5 41.0 50-51 35.04129701378456 38.0 34.0 40.0 27.0 40.5 52-53 35.13618205459353 38.5 34.5 40.0 27.5 40.5 54-55 35.471594273565096 39.0 35.0 40.0 27.5 41.0 56-57 35.27959946583731 39.0 35.0 40.0 26.5 41.0 58-59 35.047139418219516 38.0 34.5 40.0 26.0 41.0 60-61 34.766604069476294 38.0 34.0 40.0 26.0 41.0 62-63 34.44543721391986 38.0 34.0 40.0 26.0 41.0 64-65 34.07689329045975 37.0 34.0 40.0 25.0 41.0 66-67 33.705442441334355 37.0 33.0 39.5 23.5 41.0 68-69 33.22771803589784 36.0 33.0 39.0 22.5 41.0 70-71 32.79224739288193 35.5 32.0 39.0 22.0 40.0 72-73 32.39953392548079 35.0 32.0 38.0 20.5 40.0 74-75 31.940822153694775 35.0 31.5 37.0 19.5 39.5 76-77 31.504761998893017 35.0 31.0 37.0 18.0 39.0 78-79 31.024465398030276 35.0 31.0 36.0 16.5 39.0 80-81 30.554694569638826 34.0 30.5 36.0 11.5 37.5 82-83 30.084491930453424 34.0 30.0 35.0 7.5 37.0 84-85 29.650809590328844 34.0 29.5 35.0 6.0 36.5 86-87 29.219768192720277 34.0 29.0 35.0 2.0 36.0 88-89 28.832637516143485 34.0 29.0 35.0 2.0 36.0 90-91 28.43000272352688 34.0 29.0 35.0 2.0 35.5 92-93 27.980879743109917 34.0 27.5 35.0 2.0 35.0 94-95 27.46102413396238 33.0 26.5 35.0 2.0 35.0 96-97 26.841135798564437 33.0 25.5 35.0 2.0 35.0 98-99 26.088167813183627 32.5 24.5 35.0 2.0 35.0 100-101 24.18928973054655 30.5 11.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.007637615567038125 1101 2 0.10348299428105534 1101 3 0.079308689419193 1101 4 -0.01541455609497433 1101 5 0.0549277249883815 1101 6 0.047426763042800246 1101 7 0.032250917706882376 1101 8 0.0737836163109975 1101 9 0.13507622585466095 1101 10-11 0.03730772153485162 1101 12-13 0.0016583710055257939 1101 14-15 0.04595674262757399 1101 16-17 0.22702043253239168 1101 18-19 0.12247868462717548 1101 20-21 0.10712173476448328 1101 22-23 0.06331374860339167 1101 24-25 0.04646270587242185 1101 26-27 0.07546145245919433 1101 28-29 -0.06083946748040603 1101 30-31 -0.06125421271053 1101 32-33 0.041683862793696846 1101 34-35 0.06649062827564478 1101 36-37 0.0612454497418895 1101 38-39 0.1882549278059784 1101 40-41 0.16277199537221065 1101 42-43 0.21725475958707818 1101 44-45 0.317668439238723 1101 46-47 0.4097708115126082 1101 48-49 0.48548622300634037 1101 50-51 0.36644190736110005 1101 52-53 0.2947556955290409 1101 54-55 0.4973093646432005 1101 56-57 0.48645537907415815 1101 58-59 0.631366194764162 1101 60-61 0.5506299699060122 1101 62-63 0.5446140005674067 1101 64-65 0.4282415548799108 1101 66-67 0.38276876296355056 1101 68-69 0.46458834695102524 1101 70-71 0.5360806361838897 1101 72-73 0.5199521949720491 1101 74-75 0.4151166244834492 1101 76-77 0.4782215656219577 1101 78-79 0.44757967825053413 1101 80-81 0.3979012100424697 1101 82-83 0.43729341142269007 1101 84-85 0.4205972219140435 1101 86-87 0.4658670121966324 1101 88-89 0.47852431167499176 1101 90-91 0.5291317937644386 1101 92-93 0.6663916525987226 1101 94-95 0.5889169687742495 1101 96-97 0.5044988562200423 1101 98-99 0.5537405823320363 1101 100-101 0.9025178420603233 1102 1 0.12303993377755162 1102 2 0.030082105496440192 1102 3 0.14687382464770948 1102 4 0.05139266525711861 1102 5 0.034062823365964334 1102 6 -0.033865869612746735 1102 7 0.10088327265619057 1102 8 0.0520606315416714 1102 9 0.10359823880793328 1102 10-11 0.10225415892723078 1102 12-13 0.18089592980800262 1102 14-15 0.2595642752100744 1102 16-17 0.3073560499939205 1102 18-19 0.3676378877820028 1102 20-21 0.21620373334764054 1102 22-23 0.2243806658918075 1102 24-25 0.11994144808364382 1102 26-27 0.18163716750557057 1102 28-29 0.16656562603077418 1102 30-31 0.1718328062501442 1102 32-33 0.13753989764857266 1102 34-35 0.22259105598834594 1102 36-37 0.2720959904085092 1102 38-39 0.3342132856164781 1102 40-41 0.3470126105746658 1102 42-43 0.37877335505444165 1102 44-45 0.2086931626276609 1102 46-47 0.36896617641560425 1102 48-49 0.2399212105837023 1102 50-51 0.10450242682995281 1102 52-53 0.24191915473228676 1102 54-55 0.2892239724021479 1102 56-57 0.3278079666581135 1102 58-59 0.3253840822648115 1102 60-61 0.25495594435688673 1102 62-63 0.17483753475555375 1102 64-65 0.23095208218451546 1102 66-67 0.2850212426799601 1102 68-69 0.22680794401944837 1102 70-71 0.35473778142382884 1102 72-73 0.3339962382913626 1102 74-75 0.4492676097256698 1102 76-77 0.4911112795300916 1102 78-79 0.4417387466854841 1102 80-81 0.1150914207645215 1102 82-83 0.2210888095016017 1102 84-85 0.25173634722460747 1102 86-87 0.20473081039908436 1102 88-89 0.4454877439342617 1102 90-91 0.46302456590546726 1102 92-93 0.4522697100297144 1102 94-95 0.4096800514950054 1102 96-97 0.5757000534856864 1102 98-99 0.6616815860226275 1102 100-101 1.16698813174472 1103 1 0.028176161839343905 1103 2 -0.04866311769442078 1103 3 -0.047528213457525226 1103 4 -0.057141510264166584 1103 5 -0.10498889589518257 1103 6 -0.013653749050860142 1103 7 9.043403595967447E-4 1103 8 -0.024854432451476782 1103 9 0.0010699193279108954 1103 10-11 0.12417666489153589 1103 12-13 0.08512058955484036 1103 14-15 0.0036605390089405887 1103 16-17 -0.005244192284671101 1103 18-19 -0.07929646876547025 1103 20-21 -0.2091309602830833 1103 22-23 -0.3675316768563235 1103 24-25 -0.31343798710683757 1103 26-27 -8.396726510255803E-4 1103 28-29 -0.06535652880514675 1103 30-31 -0.21083500873640304 1103 32-33 -0.08511402825944003 1103 34-35 -0.06496144258275649 1103 36-37 -0.04970625408218865 1103 38-39 -0.11417396218067921 1103 40-41 0.024774479201923327 1103 42-43 -0.01592860521733286 1103 44-45 0.07616609160413645 1103 46-47 0.10216858437608067 1103 48-49 0.14482666926466692 1103 50-51 0.15703220934492634 1103 52-53 0.07067247338325444 1103 54-55 -0.01402737895447359 1103 56-57 -0.09275801329245326 1103 58-59 -0.0115752043983548 1103 60-61 -0.012010091774222076 1103 62-63 -0.09573068851528177 1103 64-65 -0.0948859930076793 1103 66-67 -0.02784868570935828 1103 68-69 0.16728778047164639 1103 70-71 0.35551660370020244 1103 72-73 0.22873516457975995 1103 74-75 0.249789132920629 1103 76-77 0.2681315845046015 1103 78-79 0.23526579234973255 1103 80-81 0.22667485374678975 1103 82-83 0.2830272723005258 1103 84-85 0.2831262499425371 1103 86-87 0.26967239628996253 1103 88-89 0.23449635243195743 1103 90-91 0.30814066260622397 1103 92-93 0.296443222074398 1103 94-95 0.3186709865303996 1103 96-97 0.4454102555210646 1103 98-99 0.6178984117049389 1103 100-101 0.8642430880194816 1104 1 0.14182641381347239 1104 2 0.017551637624038108 1104 3 0.11050152016705894 1104 4 0.011174497327480992 1104 5 0.012481761966832039 1104 6 -0.010066299462629047 1104 7 0.07662578385632202 1104 8 0.09309665543605661 1104 9 0.07672051778461508 1104 10-11 0.10767867458724112 1104 12-13 0.14510920789266635 1104 14-15 0.3184214708728419 1104 16-17 0.2071691265828548 1104 18-19 0.2688421552341609 1104 20-21 0.2867044256530136 1104 22-23 0.26123629692714445 1104 24-25 0.2217991453724224 1104 26-27 0.17472953143591496 1104 28-29 0.12559737255958936 1104 30-31 0.11897773582173699 1104 32-33 0.06563178031765204 1104 34-35 0.09605138017592196 1104 36-37 0.16892923366945212 1104 38-39 0.09221322144831134 1104 40-41 0.19137675301801238 1104 42-43 0.3320766036352012 1104 44-45 0.275310095761931 1104 46-47 0.27950451084434746 1104 48-49 0.42405455511124757 1104 50-51 0.3388307926376939 1104 52-53 0.4214746095741333 1104 54-55 0.6079282441770104 1104 56-57 0.654196472640642 1104 58-59 0.6133459417814819 1104 60-61 0.5827646993926052 1104 62-63 0.3210737162653956 1104 64-65 0.33941002312020885 1104 66-67 0.5742292902904325 1104 68-69 0.684854834043854 1104 70-71 0.558059137339491 1104 72-73 0.6659604585818357 1104 74-75 0.5555106441653415 1104 76-77 0.5060867179149113 1104 78-79 0.5901991525241748 1104 80-81 0.5519767920167595 1104 82-83 0.6424560986827537 1104 84-85 0.7483872587488065 1104 86-87 0.8484854224861778 1104 88-89 0.5518485480958937 1104 90-91 0.5397199359800062 1104 92-93 0.6200319104830037 1104 94-95 0.7717320342433744 1104 96-97 0.7774484247381217 1104 98-99 0.8508437264959703 1104 100-101 1.0741214707007742 1105 1 0.11466031684174993 1105 2 0.08635145653527232 1105 3 0.06155240438084775 1105 4 0.051875846639504175 1105 5 0.08071120691644751 1105 6 0.045216352136364435 1105 7 0.11079625193957554 1105 8 0.1844844756466273 1105 9 0.16381185204532045 1105 10-11 0.15429805122415274 1105 12-13 0.10999424425103399 1105 14-15 0.17034876349546124 1105 16-17 0.018789363252274427 1105 18-19 0.09262576568742986 1105 20-21 0.0301253756476072 1105 22-23 0.24635470367989143 1105 24-25 0.18778016424116828 1105 26-27 0.24337518045143014 1105 28-29 0.2415510204339597 1105 30-31 0.060840084313674936 1105 32-33 0.0398174895086143 1105 34-35 0.09289340636284749 1105 36-37 0.0011183577509328302 1105 38-39 0.24255172448297202 1105 40-41 0.12870795735268814 1105 42-43 0.12143615813935327 1105 44-45 0.10822157759844941 1105 46-47 0.27837071870114727 1105 48-49 0.129356091652987 1105 50-51 0.11114053789417966 1105 52-53 0.14232375530000496 1105 54-55 0.060221132597455096 1105 56-57 0.02070859512438261 1105 58-59 0.2576363444048866 1105 60-61 0.20823996548347878 1105 62-63 0.24372698265498371 1105 64-65 0.20462966230800106 1105 66-67 0.15778982092900407 1105 68-69 0.1907064336695541 1105 70-71 0.27233427600048543 1105 72-73 0.34604212540136103 1105 74-75 0.3049230049640279 1105 76-77 0.24684887068113426 1105 78-79 0.23319200406904628 1105 80-81 0.29582944752986506 1105 82-83 0.2653498263876166 1105 84-85 0.3712690622593513 1105 86-87 0.5373736533361928 1105 88-89 0.40168622503230367 1105 90-91 0.5067896834742633 1105 92-93 0.6416504747841749 1105 94-95 0.6562788050572763 1105 96-97 0.8463078646209503 1105 98-99 0.9130560242554289 1105 100-101 1.1764966907145826 1106 1 -0.09774890613897114 1106 2 -0.06759183138880331 1106 3 -0.18155785546725767 1106 4 -0.13644167999346024 1106 5 -0.14812590034624407 1106 6 -0.11542118527248135 1106 7 -0.1398313408957108 1106 8 -0.12542813480170878 1106 9 -0.12848592680727933 1106 10-11 -0.5306500120512609 1106 12-13 -0.4572892201058707 1106 14-15 -0.43938640499771253 1106 16-17 -0.4370606421712324 1106 18-19 -0.291045784652475 1106 20-21 -0.28149452736946046 1106 22-23 -0.16051399816427647 1106 24-25 -0.31198716963510975 1106 26-27 -0.2256366036681925 1106 28-29 -0.15686140419266792 1106 30-31 -0.23209912397913257 1106 32-33 -0.2035857284967122 1106 34-35 -0.3505702814957914 1106 36-37 -0.3143685707139312 1106 38-39 -0.3107998346412373 1106 40-41 -0.26657517181384804 1106 42-43 -0.2998143886565998 1106 44-45 -0.11760620701551971 1106 46-47 0.05405173527928042 1106 48-49 0.14257710657740574 1106 50-51 0.02337417491413163 1106 52-53 -0.07642307728440301 1106 54-55 -0.04409836650188481 1106 56-57 -0.10823228111836158 1106 58-59 -0.05550391809273236 1106 60-61 -0.04838899078529124 1106 62-63 -0.13550168385638983 1106 64-65 0.08204467776943147 1106 66-67 0.3049263883307205 1106 68-69 0.02902876143772204 1106 70-71 0.07764657650887585 1106 72-73 0.132382829069968 1106 74-75 0.048341671066420844 1106 76-77 0.2921578059493797 1106 78-79 0.3643145072564664 1106 80-81 0.2624421644033639 1106 82-83 0.08960882896066735 1106 84-85 0.040936179106534354 1106 86-87 0.14893608720944584 1106 88-89 0.23089739851866042 1106 90-91 0.20851649176848852 1106 92-93 0.33973653335446485 1106 94-95 0.2222316214565332 1106 96-97 0.3651858837792581 1106 98-99 0.45990143392562643 1106 100-101 0.46739347164233536 1107 1 0.07724100633231856 1107 2 0.10581071791076013 1107 3 0.17724959171547638 1107 4 0.09780547997922184 1107 5 -0.05021919188099844 1107 6 0.06743415439271416 1107 7 0.017184147340678635 1107 8 0.03228339291571558 1107 9 -0.10931600109287842 1107 10-11 0.11067238536633539 1107 12-13 0.1627202983827587 1107 14-15 0.2862635939676679 1107 16-17 0.15414199817474383 1107 18-19 -0.036232352202709706 1107 20-21 -0.06171159029494078 1107 22-23 0.20576326402869682 1107 24-25 0.14014530806885972 1107 26-27 0.16885612296495367 1107 28-29 0.11381233926337586 1107 30-31 0.1485740763022818 1107 32-33 0.1676785233707747 1107 34-35 0.02691215585807072 1107 36-37 0.029294133364537345 1107 38-39 0.2045426498912164 1107 40-41 0.08560244111473736 1107 42-43 0.06319917001660258 1107 44-45 0.0639650419757345 1107 46-47 0.3027697138419896 1107 48-49 0.17957066090521323 1107 50-51 0.050415088895960025 1107 52-53 0.13725871550223445 1107 54-55 0.21833968572564544 1107 56-57 0.21286759576206293 1107 58-59 0.44289863120682327 1107 60-61 0.45529644903096056 1107 62-63 0.2622084471262909 1107 64-65 0.3445232094391031 1107 66-67 0.44429154652386416 1107 68-69 0.4269809931094599 1107 70-71 0.4826331782015032 1107 72-73 0.38983492624132055 1107 74-75 0.426624515025285 1107 76-77 0.4754114736713575 1107 78-79 0.5015872792058964 1107 80-81 0.5632410190808734 1107 82-83 0.4465808632272257 1107 84-85 0.4629220772587068 1107 86-87 0.4989416482829654 1107 88-89 0.39888941126515576 1107 90-91 0.6474295217517287 1107 92-93 0.6795628726678942 1107 94-95 0.8994195805684164 1107 96-97 0.9812602314524064 1107 98-99 1.0211764458237376 1107 100-101 1.1601273471156723 1108 1 0.0290134443029757 1108 2 0.06468728212021091 1108 3 0.002935767577735504 1108 4 0.13855198820854042 1108 5 0.05340075014242984 1108 6 0.08788646746619122 1108 7 0.09950448785320987 1108 8 0.15026571245765297 1108 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-1.901263471536197 2316 86-87 -2.373101603300398 2316 88-89 -2.521936922926738 2316 90-91 -2.6826045718439033 2316 92-93 -2.551462921501358 2316 94-95 -2.787649116782486 2316 96-97 -2.697076648112592 2316 98-99 -2.9732621919255884 2316 100-101 -3.5747389537291987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8525.0 3 2026.0 4 1815.0 5 2034.0 6 2200.0 7 2458.0 8 3090.0 9 3713.0 10 4682.0 11 5858.0 12 7517.0 13 9072.0 14 10099.0 15 11898.0 16 14391.0 17 17786.0 18 15800.0 19 12242.0 20 12618.0 21 13656.0 22 14957.0 23 16772.0 24 18935.0 25 21645.0 26 25475.0 27 29486.0 28 35593.0 29 42984.0 30 52297.0 31 65535.0 32 83767.0 33 109654.0 34 150453.0 35 213240.0 36 321683.0 37 468233.0 38 392281.0 39 51979.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.461658254553754 18.33853831443196 14.307464379030115 23.89233905198417 2 15.230056905406322 19.39553088666215 40.8808668011869 24.493545406744623 3 19.118935126734872 23.25973217875476 29.70751390562888 27.913818788881485 4 12.495931955431407 15.69364618779635 37.23206504291319 34.57835681385905 5 13.698679002319981 36.82644355857292 35.00071343387173 14.474164005235371 6 33.18716982826118 35.27320164093951 17.4493962228111 14.090232307988204 7 29.012606733889086 31.688726727358425 20.94868896040543 18.349977578347065 8 26.211892095525457 35.156533311739295 19.96618303060603 18.66539156212922 9 25.850727510200635 16.3661631185949 20.203062126167485 37.58004724503699 10-11 24.77930735785924 26.186850169326803 28.046892124697067 20.986950348116896 12-13 25.331881556678375 23.20454316897347 28.489253720636125 22.97432155371203 14-15 22.397027991416504 24.923776596774676 26.197525996053283 26.481669415755537 16-17 22.18683126213699 28.062427453116147 27.027082336243463 22.723658948503402 18-19 22.17668205600098 26.8413842391565 29.03970025783846 21.942233447004053 20-21 22.670876194298348 26.147344908434196 29.130274450992406 22.051504446275054 22-23 22.403812133981802 25.887090812118142 29.09379307067155 22.61530398322851 24-25 22.17587545556251 26.067383099332154 29.280395493712604 22.47634595139273 26-27 22.089593351727906 26.33402278149099 29.015368144854957 22.561015721926143 28-29 22.098470472676578 26.536546531850924 29.020510171031045 22.344472824441457 30-31 22.277131311699833 26.202792566213475 28.97727010159367 22.54280602049302 32-33 22.08733890431167 26.190206896066105 29.051575265419505 22.67087893420272 34-35 22.348657730674688 26.255069682692326 28.972309574685806 22.423963011947176 36-37 22.304957446841282 26.27668827052724 28.937798087181555 22.48055619544992 38-39 22.127414178258046 26.456658818435226 28.96085411288472 22.455072890422013 40-41 22.40972279670996 26.310088728286562 28.83114871536719 22.449039759636285 42-43 22.117115214178906 26.20121666257416 28.916660725931443 22.765007397315493 44-45 22.278856406562102 26.345626019580344 28.821597258558313 22.55392031529924 46-47 22.42287935839006 26.538606089731744 28.415176095945753 22.623338455932444 48-49 22.36276223622341 26.530067054310646 28.483712615329367 22.623458094136577 50-51 22.409335831408946 26.73561460978131 28.36695568820531 22.488093870604438 52-53 22.498120989564477 26.745774014743766 28.088801537982423 22.667303457709334 54-55 22.415959077753104 26.70184971777495 28.126166952328163 22.75602425214378 56-57 22.40032473389861 26.869860030009285 28.13680625530773 22.593008980784376 58-59 22.48909329765567 26.621682363649445 28.238243874062434 22.65098046463245 60-61 22.40872114448521 26.6965585140613 28.343037549983226 22.551682791470263 62-63 22.20069408797487 26.664938744194938 28.580204389172437 22.55416277865775 64-65 22.25479307874677 26.63507215358973 28.439747924451257 22.67038684321225 66-67 22.17953670841741 26.796700303692443 28.180828567330224 22.842934420559917 68-69 22.21038373208121 26.627912783743348 28.37405952386976 22.787643960305687 70-71 22.251639222167878 26.503748852307957 28.36744891244009 22.877163013084072 72-73 22.11840370895232 26.593607944888475 28.29581059736495 22.992177748794255 74-75 22.17789985619311 26.8429547826791 28.031209790837547 22.947935570290237 76-77 22.35674706620597 26.67530332446785 28.09993381691576 22.86801579241042 78-79 22.250237950339997 26.584454439165462 28.14396974653393 23.02133786396061 80-81 22.282787465062956 26.570943072998865 28.101627274689484 23.044642187248694 82-83 22.307226214515442 26.510259516415974 28.157467328882284 23.0250469401863 84-85 22.248891407672453 26.510470796435254 28.20734966572828 23.033288130164006 86-87 22.295696808724784 26.514408687121975 28.246280321558505 22.943614182594736 88-89 22.199325173849026 26.429099584267142 28.070691086306425 23.300884155577414 90-91 22.196424890198777 26.285058783228855 28.425288855926585 23.09322747064579 92-93 22.148819385975546 26.168365347828313 28.48306272576451 23.199752540431636 94-95 22.029694856518567 26.307771134579006 28.389939854814685 23.272594154087745 96-97 22.10805963792392 26.318538249598948 28.2548216238953 23.318580488581837 98-99 21.955640157775512 26.357236393974116 28.255572041841138 23.431551406409234 100-101 22.05073748337401 25.97893696621388 28.624751792615005 23.345573757797112 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2631.0 1 1459.5 2 302.0 3 283.0 4 221.5 5 233.0 6 354.5 7 665.0 8 1081.0 9 1333.0 10 1322.5 11 1328.0 12 1472.0 13 1588.5 14 1736.5 15 1890.0 16 2035.5 17 2158.0 18 2349.5 19 2621.0 20 2956.0 21 3508.5 22 4189.5 23 5139.0 24 6297.5 25 7856.5 26 10513.0 27 13988.0 28 17012.5 29 20269.0 30 24408.0 31 28805.5 32 33343.5 33 37430.5 34 42262.5 35 47900.5 36 53767.5 37 59353.5 38 63330.0 39 66474.0 40 69373.0 41 72671.0 42 76147.0 43 79904.0 44 83995.0 45 87039.0 46 89424.5 47 91715.5 48 92485.0 49 91991.0 50 90080.0 51 86124.5 52 83355.0 53 81961.5 54 79964.0 55 73688.5 56 64337.5 57 56832.0 58 51509.0 59 45066.5 60 36896.5 61 29830.0 62 23368.0 63 17572.5 64 12766.5 65 9069.0 66 6524.5 67 4924.5 68 3705.5 69 2741.0 70 2101.5 71 1447.0 72 966.0 73 731.5 74 515.0 75 326.0 76 217.0 77 128.0 78 77.0 79 53.0 80 41.5 81 32.0 82 29.0 83 23.5 84 13.5 85 15.0 86 13.0 87 9.5 88 10.5 89 8.5 90 6.5 91 7.0 92 8.0 93 9.5 94 6.0 95 5.5 96 10.5 97 9.0 98 8.5 99 9.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2863656730186342 2 0.24924663732286095 3 0.24674274970787977 4 0.25095982358574276 5 0.2526730098486246 6 0.19095437653198385 7 0.18023598042574876 8 0.18572696203754951 9 0.17799565992813404 10-11 0.19864175078850493 12-13 0.18895565922528837 14-15 0.18414555933335092 16-17 0.18548535884663028 18-19 0.18041169183732636 20-21 0.1755357001660473 22-23 0.1771171028702459 24-25 0.169451692540172 26-27 0.1699788267749049 28-29 0.18115846533653127 30-31 0.1897902884302821 32-33 0.1848484049796614 34-35 0.19218435641302722 36-37 0.1734930550064574 38-39 0.17454732347592314 40-41 0.1714943376997619 42-43 0.1763264015181466 44-45 0.179181711956283 46-47 0.1834427136870404 48-49 0.1876597875649034 50-51 0.18937297382778526 52-53 0.20437433559122495 54-55 0.18662748302188487 56-57 0.16824367658557585 58-59 0.17085738383279303 60-61 0.1679142176888678 62-63 0.1748987462990784 64-65 0.1672772638218989 66-67 0.16543229400033385 68-69 0.1643560616044209 70-71 0.1741519727998735 72-73 0.17151630162620912 74-75 0.1743057202850039 76-77 0.17465714310815916 78-79 0.17250467831633326 80-81 0.17481089059328958 82-83 0.170747564200557 84-85 0.1650808711771786 86-87 0.16325786528206074 88-89 0.1636971438110048 90-91 0.16826564051202306 92-93 0.16541033007388664 94-95 0.1629503703117999 96-97 0.16156664294562612 98-99 0.16973722358398566 100-101 0.16090772515221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34532946209282 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04714253480665 44.18293772771313 2 12.777090786407271 14.654129614271916 3 4.2754506257133675 7.355313741913318 4 1.9286319156772374 4.423921304624736 5 1.0507724775194551 3.0128446956526336 6 0.6531037878927413 2.247147113379002 7 0.4244083118631444 1.703648412916986 8 0.3014943986993098 1.3831436499509993 9 0.22581863824364573 1.1654679787886715 >10 1.2030445772025313 12.58434651396346 >50 0.0789604124391137 3.071950510369017 >100 0.03323819532992759 3.3403330352664753 >500 4.600026576414702E-4 0.15761454863640922 >1k 3.066684384273296E-4 0.47447035115275527 >5k 7.66671096068324E-5 0.24273080140051534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5521 0.24252567583001677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4081 0.1792695676620718 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2867 0.12594115424826266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2805 0.12321762736880948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.3927852894406225E-5 0.0 0.0 0.0 6 4.3927852894406225E-5 4.3927852894406225E-5 0.0 0.0 0.0 7 8.785570578881245E-5 4.3927852894406225E-5 0.0 0.0 0.0 8 8.785570578881245E-5 4.3927852894406225E-5 0.0 0.0 0.0 9 8.785570578881245E-5 4.3927852894406225E-5 0.0 0.0 0.0 10-11 8.785570578881245E-5 4.3927852894406225E-5 0.0 0.0 0.0 12-13 8.785570578881245E-5 4.3927852894406225E-5 0.0 0.0 0.0 14-15 1.537474851304218E-4 4.3927852894406225E-5 0.0 4.3927852894406225E-5 6.589177934160934E-5 16-17 1.757114115776249E-4 4.3927852894406225E-5 0.0 4.3927852894406225E-5 8.785570578881245E-5 18-19 1.97675338024828E-4 4.3927852894406225E-5 0.0 6.589177934160934E-5 1.3178355868321867E-4 20-21 2.1963926447203112E-4 4.3927852894406225E-5 0.0 8.785570578881245E-5 1.3178355868321867E-4 22-23 2.1963926447203112E-4 4.3927852894406225E-5 0.0 1.0981963223601556E-4 1.537474851304218E-4 24-25 3.074949702608436E-4 1.3178355868321867E-4 0.0 3.733867496024529E-4 1.757114115776249E-4 26-27 3.514228231552498E-4 2.6356711736643735E-4 0.0 6.589177934160934E-4 2.1963926447203112E-4 28-29 4.612424553912654E-4 2.8553104381364043E-4 0.0 0.001361763439726593 2.4160319091923424E-4 30-31 4.832063818384685E-4 3.953506760496561E-4 0.0 0.003294588967080467 2.6356711736643735E-4 32-33 5.051703082856716E-4 4.832063818384685E-4 0.0 0.00641346652258331 3.074949702608436E-4 34-35 5.271342347328747E-4 6.149899405216871E-4 0.0 0.00931270481361412 3.514228231552498E-4 36-37 5.71062087627281E-4 6.589177934160934E-4 0.0 0.013112464088980258 3.514228231552498E-4 38-39 6.589177934160934E-4 6.589177934160934E-4 0.0 0.019921281287613224 3.514228231552498E-4 40-41 6.808817198632965E-4 6.589177934160934E-4 0.0 0.03327534856751272 3.514228231552498E-4 42-43 7.90701352099312E-4 6.589177934160934E-4 0.0 0.04520176062834401 3.95350676049656E-4 44-45 0.0010103406165713432 6.589177934160934E-4 0.0 0.058709575393373924 4.3927852894406225E-4 46-47 0.0011860520281489681 6.589177934160934E-4 0.0 0.08697714873092433 4.3927852894406225E-4 48-49 0.001361763439726593 6.589177934160934E-4 0.0 0.10663486290117112 4.3927852894406225E-4 50-51 0.001361763439726593 7.028456463104996E-4 0.0 0.13206908972703232 4.3927852894406225E-4 52-53 0.001361763439726593 7.467734992049059E-4 0.0 0.1631480456498247 4.3927852894406225E-4 54-55 0.001361763439726593 7.467734992049059E-4 0.0 0.2039350570622809 4.3927852894406225E-4 56-57 0.001361763439726593 7.90701352099312E-4 0.0 0.3011254315911547 4.612424553912654E-4 58-59 0.001361763439726593 7.90701352099312E-4 0.0 0.39113360217179305 4.832063818384685E-4 60-61 0.001383727366173796 8.126652785465152E-4 0.0 0.45827732532089294 4.832063818384685E-4 62-63 0.0014056912926209992 8.346292049937183E-4 0.0 0.5471214077998297 4.832063818384685E-4 64-65 0.0014056912926209992 8.346292049937183E-4 0.0 0.6360753099110021 4.832063818384685E-4 66-67 0.0014935469984098117 8.346292049937183E-4 0.0 0.7850126951494865 4.832063818384685E-4 68-69 0.0014935469984098117 8.346292049937183E-4 0.0 0.9529488767648014 4.832063818384685E-4 70-71 0.0015594387777514212 8.346292049937183E-4 0.0 1.0444286304174024 4.832063818384685E-4 72-73 0.0016472944835402336 8.346292049937183E-4 0.0 1.1616940337190198 5.271342347328747E-4 74-75 0.0016692584099874366 8.346292049937183E-4 0.0 1.3834418351299824 5.271342347328747E-4 76-77 0.0016692584099874366 8.346292049937183E-4 0.0 1.6413642233994887 5.271342347328747E-4 78-79 0.0016912223364346397 8.346292049937183E-4 0.0 1.8994403591541253 5.271342347328747E-4 80-81 0.001954789453801077 8.346292049937183E-4 0.0 2.118750164729448 5.930260140744841E-4 82-83 0.0019767533802482803 8.346292049937183E-4 0.0 2.447659963276315 7.028456463104996E-4 84-85 0.0020426451595898895 8.346292049937183E-4 2.1963926447203112E-5 2.9152060655579275 7.028456463104996E-4 86-87 0.002108536938931499 8.346292049937183E-4 4.3927852894406225E-5 3.2919972237596973 7.028456463104996E-4 88-89 0.002108536938931499 8.346292049937183E-4 4.3927852894406225E-5 3.8127399558964354 7.028456463104996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3275 0.0 49.07337 1 GTATCAA 5790 0.0 42.375187 1 ATCAACG 7370 0.0 32.891987 3 TCAACGC 7355 0.0 32.83343 4 CAACGCA 7540 0.0 32.02643 5 TATCAAC 7705 0.0 31.462595 2 AACGCAG 7805 0.0 30.915895 6 ACGCAGA 9075 0.0 26.118301 7 CGCAGAG 9095 0.0 25.799734 8 GTACCGT 215 1.6643753E-9 24.30218 6 GTACATG 6930 0.0 24.28905 1 TACATGG 6600 0.0 24.270737 2 ACATGGG 6855 0.0 22.39662 3 GCAGAGT 10130 0.0 22.319708 9 AGAGTAC 8930 0.0 20.824343 10-11 GAGTACT 5760 0.0 19.873575 12-13 TATTCCG 240 1.4036777E-7 19.80637 5 GGACCGT 290 2.4429028E-9 19.655056 6 CAGAGTA 9605 0.0 19.286715 10-11 AGTACTT 6235 0.0 19.197536 12-13 >>END_MODULE