FastQCFastQC Report
Thu 26 May 2016
SRR937394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276460
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54430.2390993033042531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.20009136993402038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.1346827969742495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.047.19561
GTATCAA57650.038.173041
ATCAACG73950.029.4830783
TCAACGC74250.029.2999824
CAACGCA75750.028.782495
TATCAAC77000.028.5619982
AACGCAG79100.027.621746
CGCGTAA350.00839638827.14136580-81
ACGCAGA91300.023.621757
GTACATG69350.023.576921
CGCAGAG92100.023.1586728
TACATGG68550.022.5203042
ACATGGG68450.021.9980473
AGAGTAC92100.019.72871410-11
GCAGAGT106850.019.5616329
CATGGGG43600.019.501364
AGTACTT63500.019.26328812-13
GAGTACT61550.019.10179112-13
GAGTACA53550.018.8170451
TGGTATC20250.018.2964952
Produced by FastQC (version 0.11.5)