##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937394_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25433084701686 33.0 31.0 34.0 31.0 34.0 2 32.485839856619485 34.0 31.0 34.0 31.0 34.0 3 32.574368537114644 34.0 31.0 34.0 31.0 34.0 4 36.15858394173409 37.0 35.0 37.0 35.0 37.0 5 36.01676901856391 37.0 35.0 37.0 35.0 37.0 6 36.09674670321464 37.0 35.0 37.0 35.0 37.0 7 36.05935092204563 37.0 35.0 37.0 35.0 37.0 8 36.0356518454091 37.0 35.0 37.0 35.0 37.0 9 37.75898632086661 39.0 37.0 39.0 35.0 39.0 10-11 37.678083295994654 39.0 37.0 39.0 35.0 39.0 12-13 37.593964093373046 39.0 37.0 39.0 35.0 39.0 14-15 38.87667057624557 40.0 38.0 41.0 35.0 41.0 16-17 38.84842518647373 40.0 38.0 41.0 35.0 41.0 18-19 38.8239521010692 40.0 38.0 41.0 35.0 41.0 20-21 38.71423196542 40.0 38.0 41.0 34.0 41.0 22-23 38.57961637806067 40.0 38.0 41.0 34.0 41.0 24-25 38.443379413651016 40.0 38.0 41.0 34.0 41.0 26-27 38.23899431573584 40.0 38.0 41.0 34.0 41.0 28-29 38.2334640186957 40.0 38.0 41.0 34.0 41.0 30-31 38.41457701870448 40.0 38.0 41.0 34.0 41.0 32-33 38.417372587262676 40.0 38.0 41.0 34.0 41.0 34-35 38.33018941690168 40.0 38.0 41.0 34.0 41.0 36-37 38.209615806998585 40.0 38.0 41.0 34.0 41.0 38-39 38.08480908955132 40.0 38.0 41.0 33.5 41.0 40-41 37.95263896576263 40.0 38.0 41.0 33.0 41.0 42-43 37.785804275058645 40.0 37.0 41.0 33.0 41.0 44-45 37.56546436133294 40.0 37.0 41.0 33.0 41.0 46-47 37.32002319390632 40.0 37.0 41.0 32.0 41.0 48-49 37.07797171924831 40.0 37.0 41.0 31.5 41.0 50-51 36.89893804415628 40.0 36.0 41.0 31.0 41.0 52-53 36.68652337401053 39.0 36.0 41.0 31.0 41.0 54-55 36.49441676989712 39.0 36.0 41.0 31.0 41.0 56-57 36.24936985495023 39.0 35.0 40.0 30.0 41.0 58-59 36.00198048724775 38.5 35.0 40.0 29.5 41.0 60-61 35.67552449856356 38.0 35.0 40.0 29.0 41.0 62-63 35.3098982630927 38.0 34.0 40.0 28.5 41.0 64-65 34.96836821204853 37.0 34.0 40.0 28.0 41.0 66-67 34.57930822417262 37.0 34.0 39.5 28.0 41.0 68-69 34.13922801191323 36.0 33.5 39.0 26.5 40.5 70-71 33.75765750331655 36.0 33.0 39.0 26.0 40.0 72-73 33.323250792897745 35.0 33.0 38.0 26.0 40.0 74-75 32.88464260298885 35.0 32.5 37.0 26.0 39.0 76-77 31.561884900239846 34.0 30.5 36.0 24.5 38.5 78-79 32.182441378280316 35.0 32.0 36.0 25.0 39.0 80-81 32.019213823216745 35.0 32.0 36.0 25.0 37.5 82-83 31.696134348945293 35.0 32.0 35.5 24.5 37.0 84-85 31.378732769299702 34.0 31.5 35.0 24.0 37.0 86-87 31.055908296214298 34.0 31.0 35.0 23.0 36.0 88-89 30.7192852059777 34.0 31.0 35.0 21.0 36.0 90-91 30.31021366507648 34.0 31.0 35.0 19.5 35.5 92-93 29.92201488275656 34.0 30.0 35.0 17.5 35.0 94-95 29.544531421593177 34.0 30.0 35.0 10.0 35.0 96-97 29.150773349850205 34.0 30.0 35.0 2.0 35.0 98-99 28.537283545504863 34.0 29.0 35.0 2.0 35.0 100-101 26.957658601512875 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.062571625735373 1101 2 0.11894830766955522 1101 3 0.074927788424489 1101 4 0.011514076108355198 1101 5 -0.06048726209834143 1101 6 0.014156720138750245 1101 7 0.031676085390415665 1101 8 -0.017294664675837623 1101 9 0.06989102059382901 1101 10-11 0.08084100540374095 1101 12-13 0.16696485032187525 1101 14-15 0.14055110708606122 1101 16-17 0.11168014958945349 1101 18-19 0.12819754232800307 1101 20-21 0.10005965553762053 1101 22-23 0.06951964121025611 1101 24-25 0.07642606243444305 1101 26-27 0.1303328808703057 1101 28-29 0.007848605668058894 1101 30-31 0.009178894569799922 1101 32-33 0.017973256910217117 1101 34-35 0.11340502275702846 1101 36-37 -0.013171509228911304 1101 38-39 0.04962591749931278 1101 40-41 0.026081181147532106 1101 42-43 0.02820055745574024 1101 44-45 -0.06658247052347122 1101 46-47 -0.054821978139159455 1101 48-49 -0.10355477144874214 1101 50-51 -0.134941741722038 1101 52-53 -0.05217813923493253 1101 54-55 -0.168015296306244 1101 56-57 -0.18453596870991618 1101 58-59 -0.048742787923828246 1101 60-61 -0.05084875138519607 1101 62-63 -0.17951852120805967 1101 64-65 -0.04588651160139534 1101 66-67 -0.09759114220800313 1101 68-69 -0.20031542685432413 1101 70-71 -0.265755519602223 1101 72-73 -0.3064441961509914 1101 74-75 -0.21875774784313506 1101 76-77 -0.17123226989897944 1101 78-79 -0.28275168849399535 1101 80-81 -0.3175765823572583 1101 82-83 -0.3142060040130161 1101 84-85 -0.2411057694514298 1101 86-87 -0.3808484993525134 1101 88-89 -0.338123560195168 1101 90-91 -0.1415931445669898 1101 92-93 -0.15073020513943192 1101 94-95 -0.31568665366831183 1101 96-97 -0.29738359868385444 1101 98-99 -0.4854609091165827 1101 100-101 -0.3010124193572672 1102 1 0.11112842757709274 1102 2 0.14168315786929497 1102 3 0.06463931375595422 1102 4 0.021417545191063425 1102 5 0.03333725197072113 1102 6 0.03686801727123168 1102 7 0.05627126131899729 1102 8 0.04584185238456229 1102 9 0.17045528610012894 1102 10-11 0.11027221257634068 1102 12-13 0.0998847524804205 1102 14-15 0.1122150717179693 1102 16-17 0.12680694665640857 1102 18-19 0.0431753227126066 1102 20-21 0.1360527747427227 1102 22-23 0.13385447784636284 1102 24-25 0.14738678013397788 1102 26-27 0.18242516307088152 1102 28-29 -0.007451540141005353 1102 30-31 0.11325508736769052 1102 32-33 0.06766225561159445 1102 34-35 0.10305432033491257 1102 36-37 -0.006379198709680622 1102 38-39 0.050003820506496766 1102 40-41 0.06380925411255589 1102 42-43 0.14065087377252183 1102 44-45 0.12481878118000367 1102 46-47 0.13498715036985232 1102 48-49 0.1773018087008822 1102 50-51 0.0221502976161716 1102 52-53 0.104584432100161 1102 54-55 0.1530078324392221 1102 56-57 0.1792948597806756 1102 58-59 0.077560993489044 1102 60-61 0.1259618792046524 1102 62-63 0.17902606419838918 1102 64-65 0.02358559725959708 1102 66-67 0.1434412336366293 1102 68-69 0.028956682556547264 1102 70-71 -0.035675549148947994 1102 72-73 0.01319328823478827 1102 74-75 -0.2081629458309564 1102 76-77 -0.22272028303737557 1102 78-79 -0.14968913906233894 1102 80-81 -0.013332035533853315 1102 82-83 0.08260470905838702 1102 84-85 -0.0032675836058153607 1102 86-87 -0.05624901605365551 1102 88-89 -0.003692366769495692 1102 90-91 -0.0315733764868007 1102 92-93 -0.024259810978396246 1102 94-95 -0.06708105023781386 1102 96-97 0.030850617782579803 1102 98-99 -0.16088804903188247 1102 100-101 -0.14536912277099745 1103 1 0.019844214504580293 1103 2 -0.007904753950661814 1103 3 -0.03337642069735125 1103 4 -0.051645835434065646 1103 5 -0.09163577941820478 1103 6 -0.05226494919795499 1103 7 -0.010440987014604275 1103 8 -0.03213947148651641 1103 9 -0.09332660074679211 1103 10-11 -0.06683675231667507 1103 12-13 0.027297443430860824 1103 14-15 0.012323628911907747 1103 16-17 -0.1398533151683239 1103 18-19 -0.11448347022502503 1103 20-21 -0.13561330996115117 1103 22-23 -0.09957353010474179 1103 24-25 -0.0882513859614491 1103 26-27 -0.1248801606207266 1103 28-29 -0.1457231691621601 1103 30-31 -0.11032493966919077 1103 32-33 -0.04844010221555095 1103 34-35 -0.012297305487074084 1103 36-37 0.05101517504309072 1103 38-39 -0.06740678318074345 1103 40-41 -0.06668731945957518 1103 42-43 -0.055529434557222146 1103 44-45 -0.09766104356791772 1103 46-47 -0.19701869006570405 1103 48-49 -0.21064707480837086 1103 50-51 -0.22459045063843064 1103 52-53 -0.12203100233344344 1103 54-55 -0.17260230190640158 1103 56-57 -0.18289631792345062 1103 58-59 -0.2848201836883888 1103 60-61 -0.3672445667323103 1103 62-63 -0.18063601374040417 1103 64-65 -0.10147443594154026 1103 66-67 -0.24148521471921214 1103 68-69 -0.20116837767172768 1103 70-71 -0.19141445007807079 1103 72-73 -0.15432509236107705 1103 74-75 -0.19962233295586174 1103 76-77 -0.30113564061287335 1103 78-79 -0.0869301087228962 1103 80-81 -0.11023127924231346 1103 82-83 -0.21706425489828263 1103 84-85 -0.33802822133300126 1103 86-87 -0.19101257985076003 1103 88-89 -0.19853302275535967 1103 90-91 -0.1641225881060322 1103 92-93 -0.21161704315033703 1103 94-95 -0.19706878025128916 1103 96-97 -0.23301778319823896 1103 98-99 -0.4394749718402089 1103 100-101 -0.14364010805676486 1104 1 -0.036178920465864906 1104 2 0.05110267993939743 1104 3 0.04644638927631206 1104 4 0.06522999508369054 1104 5 -0.06587547761058943 1104 6 -0.0507444907569905 1104 7 -0.016368161797643666 1104 8 -0.03637217227945655 1104 9 0.05300140985286106 1104 10-11 0.043500574599306674 1104 12-13 -0.020865763410519378 1104 14-15 0.05886581993994611 1104 16-17 -0.03722701597965283 1104 18-19 0.061574632386069084 1104 20-21 -0.07225006750367413 1104 22-23 -0.06745657965465313 1104 24-25 -0.1265771008721046 1104 26-27 -0.025533831932648354 1104 28-29 -0.12866741934193726 1104 30-31 -0.12054254621016725 1104 32-33 -0.14616193090797935 1104 34-35 -0.13619400528895653 1104 36-37 -0.05291749894101372 1104 38-39 -0.14899026660812353 1104 40-41 -0.09509264793570082 1104 42-43 -0.039981271060035795 1104 44-45 -0.0012776731828907373 1104 46-47 -0.04262726541259099 1104 48-49 -0.1067760514943572 1104 50-51 -0.010652076144971545 1104 52-53 0.014176910438116863 1104 54-55 -0.031122966494820048 1104 56-57 -0.02909962383174758 1104 58-59 -0.11123957664705131 1104 60-61 -0.13890621198254394 1104 62-63 -0.1448708340775866 1104 64-65 0.008927493521184715 1104 66-67 -0.014556550448240557 1104 68-69 -0.04993246078495872 1104 70-71 -0.004297843207069718 1104 72-73 -0.03957388717273602 1104 74-75 -0.175245299911289 1104 76-77 -0.09309405109019409 1104 78-79 -0.14295522774159508 1104 80-81 -0.05744011048304287 1104 82-83 -0.06599311079927972 1104 84-85 -0.16827915020632034 1104 86-87 -0.10629154187413903 1104 88-89 -0.08000138299270887 1104 90-91 0.014760363693209655 1104 92-93 -0.0582885844461245 1104 94-95 -0.133247029081911 1104 96-97 -0.0660670911525294 1104 98-99 0.11846523765593275 1104 100-101 0.24385351637354802 1105 1 0.012251691604021175 1105 2 -0.012938689860369834 1105 3 -0.03163134713003046 1105 4 -0.032811128083992 1105 5 -0.10168105083990042 1105 6 -0.040735719518387725 1105 7 0.027225400846560888 1105 8 -0.04764818726935971 1105 9 -0.05676914579372294 1105 10-11 -1.719858322530854E-4 1105 12-13 -0.07951829169247304 1105 14-15 -0.011583959676094935 1105 16-17 -0.08130708575209411 1105 18-19 -0.06803425503392901 1105 20-21 -0.0054259385387496195 1105 22-23 0.014755374290530199 1105 24-25 0.03034763152661668 1105 26-27 0.009106499090933085 1105 28-29 -0.17565043422143845 1105 30-31 -0.19217848505613944 1105 32-33 -0.08154675894073193 1105 34-35 -0.035027302369428526 1105 36-37 -0.08475166956807811 1105 38-39 0.0027682589175412886 1105 40-41 -0.12511757049550454 1105 42-43 -0.18121942772665278 1105 44-45 -0.04458416084884931 1105 46-47 -0.07778580204764296 1105 48-49 -0.1608672773442592 1105 50-51 -0.047712372721541385 1105 52-53 -0.013551312927482684 1105 54-55 -0.012981695993779852 1105 56-57 -0.07444957061506585 1105 58-59 0.04062735967864484 1105 60-61 0.027566371860189065 1105 62-63 0.029163427908692086 1105 64-65 0.00953478280104747 1105 66-67 -0.021280156467696543 1105 68-69 0.004796361672035232 1105 70-71 -0.03303848035453427 1105 72-73 -0.056254046396503554 1105 74-75 -0.05078756506900817 1105 76-77 -0.11777599059617572 1105 78-79 -0.08606882169078744 1105 80-81 -0.05652910203703598 1105 82-83 -0.04822899262773106 1105 84-85 -0.08537181699574248 1105 86-87 -0.04877056994896023 1105 88-89 -0.006072222842533392 1105 90-91 -0.11187621955609828 1105 92-93 -0.0465970609683275 1105 94-95 -0.09232930532713723 1105 96-97 -0.04470057450879139 1105 98-99 0.010102531885326727 1105 100-101 -0.04679245996110026 1106 1 -0.16532373956459168 1106 2 -0.06824676714798272 1106 3 -0.13671774978168116 1106 4 0.005548608529288401 1106 5 -0.09944852297108753 1106 6 -0.09112841236842684 1106 7 -0.017627204302748112 1106 8 -0.09420497314050635 1106 9 -0.1761092519877252 1106 10-11 -0.23147612085440272 1106 12-13 -0.296078329443624 1106 14-15 -0.16395432013465694 1106 16-17 -0.14760176075969156 1106 18-19 -0.11794105521957476 1106 20-21 -0.43409440592748183 1106 22-23 -0.2486276789469528 1106 24-25 -0.09556425074955399 1106 26-27 0.0022964444469053547 1106 28-29 -0.057312064882559355 1106 30-31 -0.06886466152097626 1106 32-33 -0.12488139406335108 1106 34-35 -0.1461686922447214 1106 36-37 -0.127221155686577 1106 38-39 -0.1497640287166888 1106 40-41 -0.1580101255351849 1106 42-43 -0.330792904655425 1106 44-45 -0.1912779013243764 1106 46-47 -0.14537606116668655 1106 48-49 -0.22899288526577521 1106 50-51 -0.13176530212529514 1106 52-53 -0.05558230647795881 1106 54-55 -0.028441184637308936 1106 56-57 -0.14694498953189594 1106 58-59 -0.2533510943380435 1106 60-61 -0.07391845699712007 1106 62-63 -0.023543645497106525 1106 64-65 -0.12393683137617018 1106 66-67 -0.026233716598362378 1106 68-69 0.13597982371728534 1106 70-71 -0.014937978369175653 1106 72-73 0.008287158457264354 1106 74-75 -0.12978229849041867 1106 76-77 -0.09346307527575348 1106 78-79 -0.033825080059429524 1106 80-81 -0.10365102153089012 1106 82-83 -0.1434857377781391 1106 84-85 -0.18139957778184623 1106 86-87 -0.05736915859302982 1106 88-89 0.05525016538437555 1106 90-91 -0.028560186609642102 1106 92-93 -0.16458156886086428 1106 94-95 -0.42628560077121946 1106 96-97 -0.15136023803812648 1106 98-99 -0.11541075614544383 1106 100-101 0.02588644678484897 1107 1 -0.07431121033662436 1107 2 -0.006208206922849513 1107 3 -0.02719253613299344 1107 4 -0.06433882499648291 1107 5 -0.1720497603746125 1107 6 -0.08282275507540504 1107 7 -0.038637716024972235 1107 8 -0.05636898083806585 1107 9 -0.11001498791173958 1107 10-11 0.042859395523606736 1107 12-13 0.031140797545432974 1107 14-15 -0.07754170399875449 1107 16-17 -0.046781567079143827 1107 18-19 -0.036830340712157295 1107 20-21 -0.017169084678485547 1107 22-23 -0.05399864134891885 1107 24-25 -0.029658287209500145 1107 26-27 0.12865851947741902 1107 28-29 -0.06630069960021245 1107 30-31 -0.013521375159314175 1107 32-33 -0.010418076456211622 1107 34-35 -0.016430657605702947 1107 36-37 -0.09445815570727234 1107 38-39 -0.1309210423943341 1107 40-41 -0.03498184941624771 1107 42-43 -0.02088876204363288 1107 44-45 0.03720035617143225 1107 46-47 0.11835707455454525 1107 48-49 0.047417687498928274 1107 50-51 0.07002756211208805 1107 52-53 -0.0037077073925573245 1107 54-55 -0.10065638007671396 1107 56-57 0.019555750220213497 1107 58-59 0.04981755362273077 1107 60-61 0.061374831433781196 1107 62-63 0.06936339921909251 1107 64-65 0.07171014133059828 1107 66-67 0.12118840250690255 1107 68-69 -0.01177091603482694 1107 70-71 -0.10232753286253171 1107 72-73 0.15787654430782538 1107 74-75 0.13848246551314247 1107 76-77 0.11862062017399566 1107 78-79 0.13653918318885871 1107 80-81 0.1079059238619493 1107 82-83 0.10330487616437622 1107 84-85 -0.03931971041991744 1107 86-87 0.11061587047974442 1107 88-89 0.1129324651339978 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-0.027420690080830923 2316 38-39 -0.7946822876815816 2316 40-41 -0.18591320183701754 2316 42-43 0.06858141850310773 2316 44-45 0.06029814506488407 2316 46-47 -0.005905993620203276 2316 48-49 -0.03606496085175337 2316 50-51 -4.8272195973453336E-4 2316 52-53 0.005383710726817981 2316 54-55 -0.023820116436162664 2316 56-57 0.15241874124886579 2316 58-59 0.02499766486241839 2316 60-61 0.060194620650747765 2316 62-63 0.2040026168084026 2316 64-65 0.11444845431975637 2316 66-67 0.055197386293784234 2316 68-69 0.14728192903840664 2316 70-71 0.3604948942530797 2316 72-73 0.4082616526234233 2316 74-75 0.12840805686282408 2316 76-77 0.14558566801701645 2316 78-79 0.26751831641518464 2316 80-81 0.13384194237701763 2316 82-83 0.1591748227842622 2316 84-85 0.17825466712310245 2316 86-87 0.21494787242084 2316 88-89 0.24596841272442305 2316 90-91 0.11840274542170803 2316 92-93 0.08088149012606394 2316 94-95 0.07230412163965383 2316 96-97 0.13166733120619867 2316 98-99 -0.06774333539325639 2316 100-101 -0.2521911934322283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 14.0 5 42.0 6 65.0 7 178.0 8 413.0 9 909.0 10 1449.0 11 2344.0 12 3339.0 13 4628.0 14 6056.0 15 8122.0 16 10754.0 17 14485.0 18 13472.0 19 8947.0 20 8349.0 21 8535.0 22 9027.0 23 9921.0 24 11747.0 25 14152.0 26 17409.0 27 22104.0 28 27823.0 29 35923.0 30 44723.0 31 57608.0 32 75276.0 33 100266.0 34 140706.0 35 205676.0 36 332896.0 37 545439.0 38 484188.0 39 49461.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.756396817841605 19.390887663268888 14.480569908904762 23.372145609984745 2 15.961492844152763 20.401017369073035 39.332999481651335 24.304490305122865 3 19.588923152614147 23.62211503826116 29.630698540716725 27.158263268407968 4 13.304384878275918 16.541428358064714 36.293060277799746 33.86112648585962 5 13.896105950070336 37.47268524172737 34.43711169550514 14.194097112697158 6 33.55591576394929 35.96122927703539 16.68125071382761 13.801604245187704 7 29.327069221510595 32.270323221141595 20.261502508280397 18.141105049067413 8 26.651204062447835 35.89533749769379 19.143187229294607 18.31027121056377 9 26.268065329502825 16.513709882888346 19.651520342988675 37.56670444462016 10-11 25.106854502165643 26.827222968995724 27.217587833741863 20.848334695096774 12-13 25.568167840474583 23.750566696187583 27.93164238219748 22.749623081140353 14-15 22.632244801138608 25.55074545566362 25.56183723851946 26.255172504678313 16-17 22.4222477003769 28.771535629881484 26.33900881192729 22.467207857814326 18-19 22.440741326445448 27.478431424228845 28.361491087038647 21.71933616228706 20-21 23.035392671077023 26.70009576271931 28.539728350157702 21.724783216045964 22-23 22.660611273451213 26.532225047310966 28.436041025677067 22.371122653560754 24-25 22.600720991175073 26.710322368231758 28.559891181420895 22.129065459172274 26-27 22.42798701904861 26.945182386759868 28.37706259300544 22.249768001186077 28-29 22.358362778512 27.18564420969814 28.401552359630127 22.05444065215973 30-31 22.540873065728125 26.92267481038359 28.324711676751136 22.211740447137153 32-33 22.49622220465108 26.789313231947848 28.36113966421549 22.35332489918558 34-35 22.638746123366982 26.929311299122322 28.408559781415004 22.023382796095692 36-37 22.818197592309094 26.91627991767902 28.228517585456338 22.03700490455555 38-39 22.532185494755545 27.123188381923192 28.249634024869835 22.094992098451428 40-41 22.7223897460069 26.983919546998308 28.182117889643983 22.1115728173508 42-43 22.506749764573748 26.974148253408494 28.162888826748024 22.356213155269735 44-45 22.655004404866418 27.18271508893525 28.031489248985103 22.130791257213232 46-47 22.829876211310545 27.36540505873154 27.62903806787732 22.175680662080598 48-49 22.811090120904908 27.377635041447057 27.57337768292284 22.2378971547252 50-51 22.812414889785018 27.546980838670564 27.499319118280134 22.141285153264278 52-53 22.93248300593488 27.58796401336902 27.236957965153298 22.242595015542797 54-55 22.842571360797027 27.58179366208938 27.26340458431073 22.31223039280286 56-57 22.761722147544873 27.7246031118491 27.243153844126404 22.270520896479624 58-59 22.85379492721155 27.425674072902666 27.421940205406642 22.298590794479146 60-61 22.74729184786906 27.544718554246504 27.53549370513868 22.172495892745754 62-63 22.548166890698717 27.52894845505741 27.701277421962168 22.221607232281702 64-65 22.660204879505898 27.489764810275602 27.580014584047163 22.270015726171337 66-67 22.557281920174304 27.639954139321578 27.372126020224385 22.430637920279732 68-69 22.605426846946575 27.510213225797948 27.462353830069492 22.42200609718598 70-71 22.63986628361579 27.450361526229322 27.456313750296513 22.453458439858377 72-73 22.50401939853984 27.46892104407721 27.366547182906793 22.66051237447616 74-75 22.443157358354636 27.811031162418843 27.09195417446386 22.65385730476266 76-77 22.612016024880734 27.613355824394013 27.23175456630031 22.542873584424942 78-79 22.633804239916362 27.579729053003348 27.14323994271808 22.64322676436221 80-81 22.717179304709944 27.53180376549555 27.081235778357627 22.66978115143688 82-83 22.485331866721342 27.562165364162645 27.20946685154529 22.743035917570726 84-85 22.516658547331623 27.566980121319993 27.207540307117327 22.708821024231053 86-87 22.493125291022025 27.55209843353277 27.207660138987727 22.747116136457482 88-89 22.442871827310825 27.54774957609622 27.01501454011931 22.99436405647365 90-91 22.4491956620489 27.44640163213521 27.191775887170394 22.912626818645492 92-93 22.23227217277051 27.340848978236316 27.359936074700176 23.066942774292997 94-95 22.130193370408442 27.46936032260615 27.29534013336496 23.105106173620445 96-97 22.295164421953384 27.47818982103793 27.097840506751712 23.128805250256978 98-99 22.041803192631967 27.51183981578165 26.974045753238958 23.47231123834743 100-101 21.726356402176094 27.550921782788496 27.271594996368925 23.45112681866648 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 111.0 2 113.5 3 154.0 4 152.5 5 161.5 6 273.0 7 551.0 8 875.5 9 1160.5 10 1266.5 11 1277.0 12 1439.5 13 1594.5 14 1705.5 15 1857.5 16 2010.5 17 2133.0 18 2361.5 19 2656.5 20 2965.5 21 3472.0 22 4179.5 23 5109.5 24 6199.5 25 7868.5 26 10475.5 27 13862.5 28 17027.0 29 20447.5 30 24709.5 31 28913.0 32 33178.0 33 36929.0 34 42022.5 35 47893.0 36 53961.5 37 59594.5 38 63174.0 39 66397.0 40 69369.0 41 72860.5 42 76095.0 43 80032.0 44 84744.5 45 87510.5 46 89096.5 47 91505.0 48 92902.5 49 91658.0 50 89363.5 51 86036.0 52 83617.5 53 82376.0 54 81003.5 55 75160.0 56 64781.5 57 56664.5 58 51973.5 59 45954.0 60 37357.0 61 29809.0 62 23373.5 63 17412.0 64 12237.0 65 8452.0 66 6181.0 67 4948.5 68 3848.0 69 3056.0 70 2362.5 71 1457.5 72 995.0 73 734.0 74 480.5 75 301.0 76 191.0 77 106.0 78 63.5 79 33.5 80 21.0 81 12.5 82 6.5 83 5.5 84 4.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05047310297567276 2 0.0 3 0.0 4 0.0 5 0.009576271930980557 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047442081125958725 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014056912926209992 24-25 4.612424553912654E-4 26-27 0.0020865730124842957 28-29 0.0023940679827451393 30-31 9.664127636769369E-4 32-33 0.0 34-35 0.0 36-37 2.1963926447203112E-4 38-39 3.294588967080467E-4 40-41 2.1963926447203112E-5 42-43 0.011838556355042478 44-45 2.1963926447203112E-5 46-47 0.0 48-49 6.589177934160934E-5 50-51 0.0 52-53 1.537474851304218E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.051703082856716E-4 84-85 4.3927852894406225E-5 86-87 0.0 88-89 0.0 90-91 0.007819157815204309 92-93 0.00232817620340353 94-95 0.0 96-97 0.0 98-99 0.001361763439726593 100-101 0.011992303840172901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.19207671271539 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52185327529696 41.68207919456625 2 13.5552503232394 14.96284831400578 3 4.484259774973357 7.4248682850022005 4 2.077562563016253 4.586599694138345 5 1.1584355524307572 3.196823193824759 6 0.701125212716024 2.3217933915265014 7 0.4734272776798453 1.8290604239318622 8 0.33239846878571083 1.4676609430728067 9 0.24798801976685575 1.2318276429726014 >10 1.3195792488685223 13.238895020186265 >50 0.08782791737989978 3.268111820981649 >100 0.0388577631777411 3.7618245072831282 >500 0.0010361019273530134 0.35348890974405534 >1k 3.18800593031511E-4 0.4346910659642948 >5k 7.970014825787775E-5 0.23942759279939854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5443 0.2390993033042531 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.20009136993402038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 0.1346827969742495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.3927852894406225E-5 0.0 0.0 0.0 0.0 6 8.785570578881245E-5 0.0 0.0 0.0 0.0 7 8.785570578881245E-5 0.0 0.0 0.0 0.0 8 8.785570578881245E-5 0.0 0.0 0.0 0.0 9 8.785570578881245E-5 0.0 0.0 0.0 0.0 10-11 1.3178355868321867E-4 0.0 0.0 4.3927852894406225E-5 0.0 12-13 1.3178355868321867E-4 0.0 0.0 4.3927852894406225E-5 0.0 14-15 2.1963926447203112E-4 0.0 0.0 4.3927852894406225E-5 6.589177934160934E-5 16-17 2.4160319091923424E-4 0.0 0.0 4.3927852894406225E-5 8.785570578881245E-5 18-19 2.8553104381364043E-4 0.0 0.0 4.3927852894406225E-5 8.785570578881245E-5 20-21 3.074949702608436E-4 0.0 0.0 4.3927852894406225E-5 1.0981963223601556E-4 22-23 3.514228231552498E-4 0.0 0.0 6.589177934160934E-5 1.537474851304218E-4 24-25 5.71062087627281E-4 0.0 0.0 3.294588967080467E-4 2.4160319091923424E-4 26-27 5.71062087627281E-4 0.0 0.0 6.149899405216873E-4 2.6356711736643735E-4 28-29 6.589177934160934E-4 0.0 0.0 0.0013178355868321869 2.6356711736643735E-4 30-31 7.028456463104996E-4 4.3927852894406225E-5 0.0 0.003294588967080467 2.6356711736643735E-4 32-33 7.028456463104996E-4 8.785570578881245E-5 0.0 0.006435430449030512 2.6356711736643735E-4 34-35 7.248095727577027E-4 8.785570578881245E-5 0.0 0.009466452298744541 2.6356711736643735E-4 36-37 7.467734992049059E-4 2.6356711736643735E-4 0.0 0.013705490103054743 2.6356711736643735E-4 38-39 8.785570578881245E-4 2.6356711736643735E-4 0.0 0.020931621904184565 2.6356711736643735E-4 40-41 8.785570578881245E-4 3.294588967080467E-4 0.0 0.03474693163947533 2.6356711736643735E-4 42-43 9.224849107825308E-4 3.514228231552498E-4 0.0 0.047068694376356274 2.6356711736643735E-4 44-45 0.0010323045430185463 3.514228231552498E-4 0.0 0.060862040185199834 2.6356711736643735E-4 46-47 0.0012080159545961714 3.514228231552498E-4 0.0 0.08945907241945827 2.6356711736643735E-4 48-49 0.0014056912926209992 3.514228231552498E-4 0.0 0.10916071444259948 2.6356711736643735E-4 50-51 0.0014276552190682023 3.95350676049656E-4 0.0 0.1347706526800383 2.8553104381364043E-4 52-53 0.0014496191455154056 4.3927852894406225E-4 0.0 0.16628888713177475 3.294588967080467E-4 54-55 0.0014935469984098117 4.3927852894406225E-4 0.0 0.2081301670136967 3.514228231552498E-4 56-57 0.001537474851304218 4.3927852894406225E-4 0.0 0.3065724853500611 3.733867496024529E-4 58-59 0.001537474851304218 4.3927852894406225E-4 0.0 0.3980302750762148 4.1731460249685916E-4 60-61 0.001537474851304218 4.832063818384685E-4 0.0 0.46596469957741404 4.3927852894406225E-4 62-63 0.001537474851304218 5.051703082856716E-4 0.0 0.5557532308935804 4.3927852894406225E-4 64-65 0.001537474851304218 5.271342347328747E-4 0.0 0.6455417622097467 4.3927852894406225E-4 66-67 0.001581402704198624 5.271342347328747E-4 0.0 0.7960385862259824 4.3927852894406225E-4 68-69 0.0016253305570930305 5.271342347328747E-4 0.0 0.9659075933686513 4.3927852894406225E-4 70-71 0.0016692584099874366 5.271342347328747E-4 0.0 1.058002336961774 4.3927852894406225E-4 72-73 0.0016912223364346397 5.271342347328747E-4 0.0 1.175992549836149 4.3927852894406225E-4 74-75 0.0017131862628818428 5.271342347328747E-4 0.0 1.3998488881860434 4.3927852894406225E-4 76-77 0.0017131862628818428 5.271342347328747E-4 0.0 1.6614172882457852 4.3927852894406225E-4 78-79 0.0017131862628818428 5.271342347328747E-4 0.0 1.92256837370303 4.3927852894406225E-4 80-81 0.001932825527353874 5.271342347328747E-4 0.0 2.1444040308197816 4.3927852894406225E-4 82-83 0.0020206812331426864 5.271342347328747E-4 0.0 2.4773332279064864 4.832063818384685E-4 84-85 0.0020865730124842957 5.271342347328747E-4 0.0 2.951139049225552 4.832063818384685E-4 86-87 0.002152464791825905 5.271342347328747E-4 0.0 3.333289405480439 4.832063818384685E-4 88-89 0.0022183565711675146 5.271342347328747E-4 0.0 3.86178540365304 4.832063818384685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3545 0.0 47.1956 1 GTATCAA 5765 0.0 38.17304 1 ATCAACG 7395 0.0 29.483078 3 TCAACGC 7425 0.0 29.299982 4 CAACGCA 7575 0.0 28.78249 5 TATCAAC 7700 0.0 28.561998 2 AACGCAG 7910 0.0 27.62174 6 CGCGTAA 35 0.008396388 27.141365 80-81 ACGCAGA 9130 0.0 23.62175 7 GTACATG 6935 0.0 23.57692 1 CGCAGAG 9210 0.0 23.158672 8 TACATGG 6855 0.0 22.520304 2 ACATGGG 6845 0.0 21.998047 3 AGAGTAC 9210 0.0 19.728714 10-11 GCAGAGT 10685 0.0 19.561632 9 CATGGGG 4360 0.0 19.50136 4 AGTACTT 6350 0.0 19.263288 12-13 GAGTACT 6155 0.0 19.101791 12-13 GAGTACA 5355 0.0 18.817045 1 TGGTATC 2025 0.0 18.296495 2 >>END_MODULE