FastQCFastQC Report
Thu 26 May 2016
SRR937393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.2340999035544957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43840.17421388171497354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33720.13399845099062288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.11774537671566301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.047.5980071
GTATCAA66100.042.930531
ATCAACG80150.034.564373
TCAACGC81200.034.1201364
TATCAAC83950.033.2262272
CAACGCA83650.033.122785
AACGCAG85150.032.6910976
ACGCAGA100550.027.3068087
CGCAGAG100700.027.1235338
TACATGG75900.026.0444492
GTACATG78200.026.0153941
ACATGGG76300.025.5342393
GCAGAGT113450.023.0703939
CATGGGG50800.021.983784
GAGTACT65950.021.3554712-13
AGAGTAC100650.020.08138510-11
GTACTTT69900.019.67241314-15
CAGAGTA108100.019.11487410-11
CGTATAC1759.912916E-519.0074393
CCGTCCG2752.84017E-819.0006249
Produced by FastQC (version 0.11.5)