##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937393_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2516447 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.644826614667426 33.0 31.0 34.0 30.0 34.0 2 31.74623864520095 33.0 31.0 34.0 30.0 34.0 3 31.80165050167955 33.0 31.0 34.0 30.0 34.0 4 35.41930984439569 37.0 35.0 37.0 33.0 37.0 5 35.400549663871324 37.0 35.0 37.0 33.0 37.0 6 35.443545602192295 37.0 35.0 37.0 33.0 37.0 7 35.38316284825391 37.0 35.0 37.0 33.0 37.0 8 35.36254012105162 37.0 35.0 37.0 33.0 37.0 9 36.98119412012254 39.0 37.0 39.0 33.0 39.0 10-11 36.88416127977263 39.0 37.0 39.0 33.0 39.0 12-13 36.76657843379972 39.0 37.0 39.0 33.0 39.0 14-15 37.94991748286374 40.0 38.0 41.0 33.0 41.0 16-17 37.88498188119996 40.0 38.0 41.0 33.0 41.0 18-19 37.81081858668193 40.0 38.0 41.0 33.0 41.0 20-21 37.721434625883234 40.0 37.5 41.0 32.0 41.0 22-23 37.58256442515976 40.0 37.0 41.0 32.0 41.0 24-25 37.441871217633434 40.0 37.0 41.0 32.0 41.0 26-27 37.27369859170489 39.5 37.0 41.0 31.5 41.0 28-29 37.09565589897184 39.0 36.5 41.0 31.0 41.0 30-31 37.1091813179455 39.0 37.0 41.0 31.0 41.0 32-33 36.981612765935466 39.0 36.0 41.0 31.0 41.0 34-35 36.79523808766884 39.0 36.0 41.0 30.0 41.0 36-37 36.860959320820186 39.0 36.0 41.0 30.0 41.0 38-39 36.85744583533848 39.5 36.0 41.0 30.0 41.0 40-41 36.72371601706692 39.5 36.0 41.0 30.0 41.0 42-43 36.522249822865334 39.0 36.0 41.0 30.0 41.0 44-45 36.35077412717216 39.0 36.0 41.0 30.0 41.0 46-47 36.09992342377964 39.0 35.0 41.0 29.0 41.0 48-49 35.846466466410774 39.0 35.0 41.0 28.0 41.0 50-51 34.93649538416665 38.0 34.0 39.5 27.0 40.5 52-53 35.07708646357344 38.0 34.5 39.5 27.0 40.5 54-55 35.44574493323324 39.0 35.0 40.0 27.0 41.0 56-57 35.23931638536396 38.5 35.0 40.0 26.5 41.0 58-59 34.996182911859464 38.0 34.0 40.0 26.0 41.0 60-61 34.721492842885226 38.0 34.0 40.0 26.0 41.0 62-63 34.406533696119965 38.0 34.0 40.0 25.5 41.0 64-65 34.053801649706905 37.0 34.0 40.0 25.0 41.0 66-67 33.66532158237388 36.5 33.0 39.5 23.5 41.0 68-69 33.176882723935776 36.0 32.5 39.0 22.0 41.0 70-71 32.75493443732374 35.5 32.0 39.0 22.0 40.0 72-73 32.3449919668485 35.0 32.0 38.0 20.5 40.0 74-75 31.887495941698752 35.0 31.0 37.0 19.5 39.5 76-77 31.443740718560733 35.0 31.0 37.0 18.0 39.0 78-79 30.969548732796678 35.0 31.0 36.0 16.0 39.0 80-81 30.5321377720254 34.0 30.5 36.0 11.5 37.5 82-83 30.10210288553663 34.0 30.0 35.0 7.5 37.0 84-85 29.647783164119886 34.0 29.5 35.0 6.0 36.5 86-87 29.217386060584623 34.0 29.0 35.0 2.0 36.0 88-89 28.84527053420954 34.0 29.0 35.0 2.0 36.0 90-91 28.43059162382518 34.0 29.0 35.0 2.0 35.5 92-93 27.99168490335779 34.0 27.5 35.0 2.0 35.0 94-95 27.467295953381893 33.0 26.5 35.0 2.0 35.0 96-97 26.859880021315767 33.0 25.5 35.0 2.0 35.0 98-99 26.15813843883857 33.0 25.0 35.0 2.0 35.0 100-101 24.343523030685724 30.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06348944064870565 1101 2 -0.0459613861499264 1101 3 -0.009701086399601877 1101 4 0.028707463456747462 1101 5 0.05401326720089372 1101 6 -0.07720481452597738 1101 7 0.0948258374172326 1101 8 -0.03737282581173673 1101 9 0.052294457561302465 1101 10-11 0.05105136855294745 1101 12-13 0.12755268276855247 1101 14-15 0.16658599751343672 1101 16-17 0.11552364971020523 1101 18-19 0.18662779970094334 1101 20-21 0.18529811553966624 1101 22-23 0.1255820925938238 1101 24-25 0.16232918293397347 1101 26-27 0.22817767703669034 1101 28-29 0.16223585199771406 1101 30-31 0.11775732552399631 1101 32-33 0.0491039290744979 1101 34-35 0.019051944338656313 1101 36-37 0.13383029511204825 1101 38-39 0.2262039140821699 1101 40-41 0.1750799451922731 1101 42-43 0.2511894486183053 1101 44-45 0.11250524345579294 1101 46-47 0.30333544126420264 1101 48-49 0.4182948847541468 1101 50-51 0.3730748019625878 1101 52-53 0.30183472715681603 1101 54-55 0.36952433609737767 1101 56-57 0.4870112232356263 1101 58-59 0.5082733029025093 1101 60-61 0.4496920993187672 1101 62-63 0.5060102078067814 1101 64-65 0.33847415524472524 1101 66-67 0.49722353653954343 1101 68-69 0.36933820857134236 1101 70-71 0.32191225685861014 1101 72-73 0.4065831000460207 1101 74-75 0.43506100932530245 1101 76-77 0.4633837727598866 1101 78-79 0.5721922398453181 1101 80-81 0.6123987630243697 1101 82-83 0.5709606066377297 1101 84-85 0.4715795659903961 1101 86-87 0.5440823768467524 1101 88-89 0.6321524139310277 1101 90-91 0.7451958871066324 1101 92-93 0.7139960998394024 1101 94-95 0.6394057404881686 1101 96-97 0.6406064487894625 1101 98-99 0.7181271284006492 1101 100-101 1.0452788021776342 1102 1 0.0022676593191235384 1102 2 -0.11091167525446366 1102 3 -0.024013114940657942 1102 4 -0.24865697910238538 1102 5 -0.10919154800621556 1102 6 -0.14610325499248944 1102 7 0.05740583353629347 1102 8 -0.001769516307746244 1102 9 0.12718128485091285 1102 10-11 -0.09470233397721017 1102 12-13 -0.00882788730473294 1102 14-15 0.14904148753419122 1102 16-17 0.09987367862387941 1102 18-19 0.11309587796964848 1102 20-21 0.08209679608317799 1102 22-23 0.17208891261149262 1102 24-25 0.2213680433031513 1102 26-27 0.23482089257284855 1102 28-29 0.181254250379844 1102 30-31 0.19351486709425103 1102 32-33 0.1342419765728735 1102 34-35 0.15340211024579276 1102 36-37 0.24211153332657886 1102 38-39 0.3438519185871627 1102 40-41 0.23027702558777463 1102 42-43 0.1457484473476569 1102 44-45 0.21745339062737656 1102 46-47 0.2622509833489133 1102 48-49 0.3391974024682085 1102 50-51 0.26927453906409227 1102 52-53 0.20821960527131722 1102 54-55 0.2292632652533868 1102 56-57 0.17115141846903015 1102 58-59 0.06386371568151361 1102 60-61 0.17304131207379214 1102 62-63 0.2085644588264941 1102 64-65 0.17733935142901913 1102 66-67 0.28787332228177576 1102 68-69 0.32125531915052363 1102 70-71 0.23918573737556414 1102 72-73 0.1355926405925345 1102 74-75 0.10493813103157379 1102 76-77 0.19769657168766486 1102 78-79 0.2703583487605954 1102 80-81 0.06842370292453381 1102 82-83 0.18973921006521266 1102 84-85 0.1546531463030476 1102 86-87 0.08038773751345474 1102 88-89 0.24915985315911726 1102 90-91 0.02580441525540067 1102 92-93 -0.1660003334957132 1102 94-95 -0.09896182294302136 1102 96-97 0.023564156814828152 1102 98-99 0.3412649038461346 1102 100-101 0.5587868403088763 1103 1 0.0721546723507025 1103 2 0.08910567994509933 1103 3 0.05483855155443962 1103 4 -0.0059953249868911485 1103 5 -0.06777927029134645 1103 6 0.0036467113529425887 1103 7 0.06380020174103862 1103 8 0.07783013949179463 1103 9 0.1592669575067731 1103 10-11 0.09172187461248882 1103 12-13 0.1203002415803951 1103 14-15 0.14952123012292873 1103 16-17 0.1814507053548411 1103 18-19 0.05545797342193026 1103 20-21 0.1008260589293215 1103 22-23 0.14332596880010584 1103 24-25 0.058591448934414814 1103 26-27 -0.032068422167327526 1103 28-29 -0.1936711816524408 1103 30-31 -0.20328009607049324 1103 32-33 -0.19080474388831448 1103 34-35 0.0075027875604973815 1103 36-37 0.08230116032837032 1103 38-39 -0.032653463504381364 1103 40-41 0.1248695941309137 1103 42-43 0.043548263369345364 1103 44-45 -0.04455353693479225 1103 46-47 0.032053892951424245 1103 48-49 0.09407517039157653 1103 50-51 0.06813866434883664 1103 52-53 0.14183926640279765 1103 54-55 0.1749352216265052 1103 56-57 0.26394482032569755 1103 58-59 0.17639139821466898 1103 60-61 0.237414584802508 1103 62-63 0.24239653968192698 1103 64-65 0.2073551030873375 1103 66-67 0.37264604835470294 1103 68-69 0.34279341876277414 1103 70-71 0.2772290936995816 1103 72-73 0.1907445612786134 1103 74-75 0.36940959591599665 1103 76-77 0.30593527629028827 1103 78-79 0.4772920479388958 1103 80-81 0.30411655152648365 1103 82-83 0.3261865479227737 1103 84-85 0.3126433680008738 1103 86-87 0.41228283292085877 1103 88-89 0.45426581226155704 1103 90-91 0.5074382588184037 1103 92-93 0.44212371460788447 1103 94-95 0.5041772245402747 1103 96-97 0.6749562681837382 1103 98-99 0.7689686195745686 1103 100-101 1.021056108539753 1104 1 -0.02912065006495368 1104 2 0.08890525847958486 1104 3 0.024870591674240217 1104 4 -0.12959322831204645 1104 5 -0.014751916004925647 1104 6 -0.011192330065668443 1104 7 -0.08585727217003125 1104 8 0.05217619190593581 1104 9 -0.03522927101876405 1104 10-11 -0.030354088878709717 1104 12-13 0.08592318017562661 1104 14-15 0.1336309149524837 1104 16-17 0.05378018322107181 1104 18-19 0.002174885442713048 1104 20-21 -0.017767453560992408 1104 22-23 0.025661838301495266 1104 24-25 -0.01941910682372594 1104 26-27 0.062174223696480624 1104 28-29 0.08967167767558948 1104 30-31 0.10215129150159896 1104 32-33 0.07351292857315883 1104 34-35 0.0979942742575659 1104 36-37 -0.09350380329530594 1104 38-39 0.03687315260281565 1104 40-41 -0.01357469143314205 1104 42-43 0.08063545319407694 1104 44-45 0.10472784797412515 1104 46-47 0.07414308357648025 1104 48-49 0.13462398377358653 1104 50-51 -0.0027821954626645606 1104 52-53 0.039471015348112815 1104 54-55 0.024925729775169714 1104 56-57 0.112573520952715 1104 58-59 -0.017403013748072738 1104 60-61 0.11046175588936791 1104 62-63 0.10058913412584758 1104 64-65 0.03530266436440144 1104 66-67 0.07838482850839767 1104 68-69 -8.725459166143423E-4 1104 70-71 0.005770851699708146 1104 72-73 0.060650714866326894 1104 74-75 0.20118718749870723 1104 76-77 0.13160923187935225 1104 78-79 0.11290499618986871 1104 80-81 0.2029095158111751 1104 82-83 0.3113457993366495 1104 84-85 0.33253050374254656 1104 86-87 0.2590235770000575 1104 88-89 0.21974060286039432 1104 90-91 0.3924926218605478 1104 92-93 0.26948691244674805 1104 94-95 0.4248099827630156 1104 96-97 0.5669130508968223 1104 98-99 0.678151746750352 1104 100-101 0.7238824609237113 1105 1 0.15739315143554578 1105 2 0.20820916094509556 1105 3 0.25832869853507745 1105 4 0.23869227684479455 1105 5 0.27487398460657886 1105 6 0.22569609364552434 1105 7 0.19381117812466186 1105 8 0.1984525919131741 1105 9 0.3055115479218742 1105 10-11 0.28582549853415173 1105 12-13 0.3402941245332727 1105 14-15 0.3726793986675858 1105 16-17 0.32558362821716713 1105 18-19 0.4331210327458521 1105 20-21 0.32154641268902395 1105 22-23 0.4472018613458886 1105 24-25 0.37804022866927056 1105 26-27 0.3884185405186855 1105 28-29 0.4585259530540782 1105 30-31 0.3127935886214601 1105 32-33 0.27897375612025854 1105 34-35 0.3708628186602496 1105 36-37 0.34760263928172463 1105 38-39 0.31084188582474326 1105 40-41 0.35023761740999504 1105 42-43 0.37828365820472243 1105 44-45 0.39204041119036503 1105 46-47 0.20317957254392383 1105 48-49 0.2559155154616306 1105 50-51 0.22704968703908435 1105 52-53 0.2820105437222722 1105 54-55 0.3941024456876576 1105 56-57 0.42273964770492256 1105 58-59 0.4790907596406768 1105 60-61 0.5570187203837094 1105 62-63 0.6236102018494236 1105 64-65 0.4908486516035211 1105 66-67 0.6214052024871322 1105 68-69 0.6213511676894328 1105 70-71 0.5345493982942315 1105 72-73 0.612914283610408 1105 74-75 0.6379132347015144 1105 76-77 0.4744669277215081 1105 78-79 0.6964346894742164 1105 80-81 0.6426119294399406 1105 82-83 0.6311486450980723 1105 84-85 0.689345993757648 1105 86-87 0.6678880775741582 1105 88-89 0.6992232501906805 1105 90-91 0.5998088146693306 1105 92-93 0.5261244921017258 1105 94-95 0.6938705718227602 1105 96-97 0.7136916115673237 1105 98-99 0.7962762327256421 1105 100-101 0.9776170703453673 1106 1 0.09292351643885866 1106 2 0.04163400533478878 1106 3 0.0021691359489821593 1106 4 0.06393042590755726 1106 5 0.06238122470847429 1106 6 0.006849541658233704 1106 7 0.06616800610750317 1106 8 0.10401011647843461 1106 9 0.07797342523320339 1106 10-11 -0.00255202256991538 1106 12-13 -0.11433944488965153 1106 14-15 -0.15854630667958958 1106 16-17 -0.1704666447514427 1106 18-19 -0.054770004598701405 1106 20-21 0.026071069267125324 1106 22-23 -0.048293725624425576 1106 24-25 0.019298496115283115 1106 26-27 0.13984564682157696 1106 28-29 0.2896937375860986 1106 30-31 0.2849775481956698 1106 32-33 0.29572928366265927 1106 34-35 0.24925477845924604 1106 36-37 0.032035222837450306 1106 38-39 0.24915602610295906 1106 40-41 0.41503031900412424 1106 42-43 0.34477164522331094 1106 44-45 0.3637216666224319 1106 46-47 0.3158195292214572 1106 48-49 0.5104038038983489 1106 50-51 0.35617734336621254 1106 52-53 0.48313185749663745 1106 54-55 0.5518359601273133 1106 56-57 0.3783214709142442 1106 58-59 0.32293758303200804 1106 60-61 0.3859146934502107 1106 62-63 0.5223261095107361 1106 64-65 0.3885720506549504 1106 66-67 0.4488993896511957 1106 68-69 0.3823715447047604 1106 70-71 0.34034761034827454 1106 72-73 0.5331718811673909 1106 74-75 0.4733371454613007 1106 76-77 0.29984997461182417 1106 78-79 0.3817452810647808 1106 80-81 0.4362936433851914 1106 82-83 0.27895119833955917 1106 84-85 0.3617816247017913 1106 86-87 0.5267132109931936 1106 88-89 0.6164180691194865 1106 90-91 0.7214812893824565 1106 92-93 0.5710041834802091 1106 94-95 0.6188767821857972 1106 96-97 0.6726211557170991 1106 98-99 0.819036941850122 1106 100-101 1.0138424212707946 1107 1 0.11067462974909859 1107 2 -0.04415594472510165 1107 3 0.027503027627343357 1107 4 -0.03109638276531257 1107 5 0.10619593121907656 1107 6 0.11538968571936437 1107 7 -0.04858119152500251 1107 8 0.1156436001458303 1107 9 0.08104702673438169 1107 10-11 0.11267094028189462 1107 12-13 0.10128836880426917 1107 14-15 0.1732342562890281 1107 16-17 0.14294058142291277 1107 18-19 0.15429717052696645 1107 20-21 0.14286488448060908 1107 22-23 0.10768267855248581 1107 24-25 0.23661123084325908 1107 26-27 0.22159038377753149 1107 28-29 0.21732460054686698 1107 30-31 0.17603377002492948 1107 32-33 0.12383970822119039 1107 34-35 0.18653314725357006 1107 36-37 0.23798384495547964 1107 38-39 0.3641843962492075 1107 40-41 0.38184757321739937 1107 42-43 0.2686385799767095 1107 44-45 0.34974048068678343 1107 46-47 0.4688020109772282 1107 48-49 0.33585287958722887 1107 50-51 0.2589812865258807 1107 52-53 0.2517426261200839 1107 54-55 0.36197304870273683 1107 56-57 0.4274625084626038 1107 58-59 0.3964303387418582 1107 60-61 0.4469317162139248 1107 62-63 0.5452438577174092 1107 64-65 0.455641810661227 1107 66-67 0.45625698801050163 1107 68-69 0.33313392901378336 1107 70-71 0.4463949736889319 1107 72-73 0.47147036187406854 1107 74-75 0.4252281336644117 1107 76-77 0.4261448652816462 1107 78-79 0.5665095763197918 1107 80-81 0.455954350766433 1107 82-83 0.5349192712786177 1107 84-85 0.5542516390051588 1107 86-87 0.3782976164030529 1107 88-89 0.5350651369293402 1107 90-91 0.6670739969637829 1107 92-93 0.4976699409773069 1107 94-95 0.5496668403996878 1107 96-97 0.8897169064816133 1107 98-99 1.0747659775490526 1107 100-101 0.9299944269867311 1108 1 -0.08290242684356741 1108 2 -0.15019682686885716 1108 3 -0.09211386322057535 1108 4 -0.13281737241797487 1108 5 -0.09306494508326324 1108 6 -0.08808448271422264 1108 7 -0.04099946736938875 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-1.503342049646566 2316 94-95 -1.3770085412567603 2316 96-97 -1.5407216979179559 2316 98-99 -1.5646250249752889 2316 100-101 -1.9278317840963304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9777.0 3 2101.0 4 1875.0 5 2166.0 6 2447.0 7 2867.0 8 3335.0 9 4309.0 10 5384.0 11 6550.0 12 8414.0 13 10050.0 14 11342.0 15 13154.0 16 15800.0 17 19537.0 18 17454.0 19 13854.0 20 14213.0 21 15279.0 22 17099.0 23 18702.0 24 21157.0 25 24549.0 26 28435.0 27 33419.0 28 40302.0 29 48761.0 30 59723.0 31 74518.0 32 95097.0 33 123613.0 34 168219.0 35 237456.0 36 353996.0 37 508159.0 38 425369.0 39 57955.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35278772058522 18.40537513283825 14.272974791911638 23.96886235466489 2 15.221030172853355 19.306379703752803 40.91798866765245 24.55460145574138 3 19.139069523607688 23.226774341070573 29.723330109262534 27.910826026059205 4 12.550068936285822 15.64955270090416 37.13552503317347 34.66485332963655 5 13.777490632482214 36.720789217605635 35.005658152020864 14.496061997891289 6 33.16651437967437 35.24896434891836 17.469686925813342 14.11483434559393 7 29.075246811213063 31.76503612332697 20.86174391358273 18.29797315187724 8 26.28592792377421 35.18192253436169 19.866889155121196 18.665260386742904 9 25.905847878710663 16.371631736526947 20.19222193910052 37.53029844566187 10-11 24.848813292604092 26.165956864777414 27.96715672230603 21.018073120312465 12-13 25.303575492248125 23.270135848676443 28.435423747407036 22.990864911668396 14-15 22.427280172343433 24.94021050837889 26.174240931246963 26.458268388030714 16-17 22.219010339210683 28.054530606524292 26.95375724614743 22.772701808117596 18-19 22.195806865723398 26.927320744335777 28.924894990497833 21.95197739944299 20-21 22.73028771407356 26.157594771658182 29.050800355934935 22.061317158333317 22-23 22.42738227831974 25.969468855226395 28.98310803749257 22.62004082896129 24-25 22.25655059624345 26.09262530050045 29.15755569148893 22.493268411767176 26-27 22.109951643159125 26.390659809793636 28.931290126911076 22.568098420136163 28-29 22.205133482938322 26.533293546607702 28.915958372043445 22.345614598410535 30-31 22.301739499329816 26.25350198189331 28.913525110451904 22.531233408324965 32-33 22.16444521598425 26.191823288362826 28.943665560851926 22.700065934800993 34-35 22.38566381761391 26.317193390080824 28.85787137001569 22.439271422289575 36-37 22.40754041212766 26.319818962586183 28.779482479518233 22.493158145767925 38-39 22.16725882095575 26.497015076595222 28.865486259558853 22.470239842890177 40-41 22.486235827515447 26.321731796547226 28.707767195337492 22.484265180599838 42-43 22.15579470346975 26.266721471857768 28.795655925579062 22.781827899093425 44-45 22.272640585813875 26.396720722743726 28.730165934678027 22.600472756764376 46-47 22.440380785856526 26.58285221537205 28.320231478053298 22.65653552071813 48-49 22.45558736631221 26.540900710227895 28.355805901034703 22.64770602242519 50-51 22.469489665584756 26.781866437756207 28.27356020525496 22.47508369140407 52-53 22.529490824512745 26.78246459339164 28.016785974445963 22.671258607649648 54-55 22.48671097006932 26.71459344527309 28.03567233532553 22.763023249332065 56-57 22.415968878807448 26.905006636183483 28.028201590295488 22.650822894713585 58-59 22.57868115678671 26.679035892981645 28.12717878740051 22.615104162831138 60-61 22.4550845760687 26.713824328751723 28.2562214395786 22.574869655600978 62-63 22.25575918356298 26.663781833352708 28.507102740936663 22.573356242147653 64-65 22.324908077350177 26.654473343377 28.34979511594306 22.670823463329764 66-67 22.25585007128334 26.808767552602568 28.092663966618453 22.842718409495635 68-69 22.208313594980954 26.679319116411826 28.29946298412041 22.812904304486807 70-71 22.303902346392114 26.513207736457556 28.300341693674785 22.882548223475546 72-73 22.217528585013703 26.573885270563522 28.17520741640686 23.03337872801591 74-75 22.224153014274922 26.895316236866385 27.983227985736026 22.897302763122667 76-77 22.420449211588554 26.695392151437424 27.981649184675835 22.90250945229819 78-79 22.331160582020797 26.658575745789292 28.002256487101835 23.008007185088076 80-81 22.313756454629647 26.6445957460699 27.99339265664525 23.0482551426552 82-83 22.34588505932007 26.560299683920896 28.061844645467062 23.031970611291975 84-85 22.349557938701974 26.513327721688977 28.091269301804093 23.045845037804956 86-87 22.346668726104813 26.60220174107448 28.11877491031094 22.932354622509767 88-89 22.312438754506264 26.42598618307633 27.94633745229495 23.31523761012246 90-91 22.244782959448994 26.307586690921063 28.294342580537418 23.153287769092522 92-93 22.18648211954225 26.211911626154322 28.418570459149624 23.183035795153806 94-95 22.061069082172242 26.331505646785057 28.340461277695223 23.266963993347474 96-97 22.142875909514682 26.322543225583527 28.185361461178783 23.34921940372301 98-99 22.050650503326214 26.317094818565494 28.223569874991018 23.40868480311727 100-101 22.05566002735551 26.02889684679162 28.570746162187294 23.344696963665577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3015.0 1 1697.5 2 356.0 3 336.0 4 283.5 5 265.0 6 408.0 7 710.5 8 1165.5 9 1473.5 10 1467.0 11 1468.5 12 1549.0 13 1632.5 14 1844.5 15 2041.0 16 2152.0 17 2341.5 18 2548.0 19 2763.0 20 3110.5 21 3734.0 22 4557.0 23 5551.5 24 6781.5 25 8587.5 26 11503.5 27 15255.5 28 18535.0 29 22232.0 30 26757.5 31 31330.5 32 36649.0 33 41380.5 34 46470.0 35 52629.5 36 59215.0 37 65140.5 38 69361.0 39 72914.0 40 76215.0 41 80245.5 42 83963.0 43 88002.5 44 92938.0 45 96194.5 46 98842.0 47 101952.0 48 102553.5 49 101643.5 50 99624.0 51 95530.5 52 92764.5 53 91185.5 54 88806.0 55 81928.5 56 71700.5 57 63274.5 58 57549.0 59 50327.0 60 40902.5 61 32993.0 62 26041.5 63 19605.0 64 14021.0 65 10060.5 66 7306.0 67 5483.5 68 4258.5 69 3247.5 70 2439.0 71 1610.0 72 1095.5 73 820.5 74 540.5 75 334.0 76 224.5 77 147.0 78 98.5 79 61.0 80 47.0 81 32.0 82 20.5 83 16.5 84 10.5 85 9.5 86 12.5 87 10.5 88 10.0 89 9.5 90 8.0 91 6.0 92 5.5 93 5.5 94 3.0 95 2.5 96 1.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27085807887072527 2 0.2428423884945719 3 0.24490879402586266 4 0.24471010118631548 5 0.234854936344775 6 0.1884402890265521 7 0.1951958455711565 8 0.19297048576822798 9 0.18943375322428804 10-11 0.20129571574525512 12-13 0.20213022567135328 14-15 0.18748656339672562 16-17 0.19630852547262073 18-19 0.1848042100628386 20-21 0.1888376747056465 22-23 0.1887383282858729 24-25 0.18261858882781956 26-27 0.19100342665671083 28-29 0.18762564838440865 30-31 0.19207636799026565 32-33 0.19372551855850728 34-35 0.18583741282848396 36-37 0.1868110077422652 38-39 0.18174434033381193 40-41 0.1820622488770874 42-43 0.1795984576667023 44-45 0.19058617169366174 46-47 0.1823602881364082 48-49 0.18748656339672562 50-51 0.19229493011376755 52-53 0.1965270875961226 54-55 0.19777885248526988 56-57 0.1772737514440002 58-59 0.17081623415871663 60-61 0.17675715006117754 62-63 0.17719427430818135 64-65 0.17550538517203024 66-67 0.1843869550997895 68-69 0.17552525445598496 70-71 0.18073100685212126 72-73 0.18965231534778998 74-75 0.17979715050624948 76-77 0.1837511380132385 78-79 0.18192316388940438 80-81 0.1745913981101132 82-83 0.18812238048327662 84-85 0.17518747662875475 86-87 0.17624054867835484 88-89 0.17991636620997778 90-91 0.17733335929586438 92-93 0.1844068243837442 94-95 0.18357231445764605 96-97 0.17457152882615845 98-99 0.17912159485178905 100-101 0.17242564615904885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2516447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.07888755736177 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35890537913215 42.82122468759579 2 13.051194723724166 14.637929628019213 3 4.30163518210118 7.236927470695304 4 2.058239897406444 4.616952150909272 5 1.1110082914385497 3.1152054525439525 6 0.6956205562273393 2.3405776173157444 7 0.4666876052719864 1.831992521832351 8 0.3245559057242885 1.4560587314552065 9 0.23358285801245085 1.178916014682669 >10 1.2766785824712648 12.995282397797189 >50 0.08471761792280913 3.238545299510556 >100 0.03618044781901186 3.6010273161753843 >500 6.383267668459397E-4 0.22285406429807972 >1k 2.8370078526163324E-4 0.47219765327895186 >5k 7.092519631540831E-5 0.23430899389035592 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.2340999035544957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4384 0.17421388171497354 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3372 0.13399845099062288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2963 0.11774537671566301 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.973856790943739E-5 0.0 0.0 0.0 3 0.0 3.973856790943739E-5 0.0 0.0 0.0 4 0.0 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 5 0.0 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 6 0.0 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 7 1.1921570372831219E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 8 1.5895427163774956E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 9 1.5895427163774956E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 10-11 1.5895427163774956E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 12-13 2.1856212350190567E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 14-15 2.781699753660618E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 16-17 2.781699753660618E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 18-19 3.179085432754991E-4 3.973856790943739E-5 0.0 3.973856790943739E-5 0.0 20-21 3.5764711118493653E-4 3.973856790943739E-5 0.0 5.9607851864156086E-5 0.0 22-23 4.3712424700381134E-4 3.973856790943739E-5 0.0 1.788235555924683E-4 1.9869283954718695E-5 24-25 4.7686281491324874E-4 1.1921570372831219E-4 0.0 4.1725496304909266E-4 3.973856790943739E-5 26-27 4.7686281491324874E-4 1.1921570372831219E-4 0.0 5.762092346868422E-4 3.973856790943739E-5 28-29 5.166013828226861E-4 1.1921570372831219E-4 0.0 0.001450457728694465 3.973856790943739E-5 30-31 5.960785186415609E-4 1.788235555924683E-4 0.0 0.003477124692075772 5.9607851864156086E-5 32-33 6.159478025962795E-4 2.3843140745662437E-4 0.0 0.005980654470370328 7.947713581887478E-5 34-35 6.55686370505717E-4 2.3843140745662437E-4 0.0 0.00884183135984982 1.1921570372831219E-4 36-37 7.152942223698731E-4 2.781699753660618E-4 0.0 0.012418302471699186 1.1921570372831219E-4 38-39 7.550327902793105E-4 2.781699753660618E-4 0.0 0.018061179114839294 1.1921570372831219E-4 40-41 7.749020742340291E-4 3.179085432754991E-4 0.0 0.03107556010518004 1.1921570372831219E-4 42-43 8.742484940076226E-4 3.179085432754991E-4 0.0 0.044586673194388755 1.1921570372831219E-4 44-45 9.537256298264975E-4 3.179085432754991E-4 0.0 0.05956811329624665 1.1921570372831219E-4 46-47 0.001112679901464247 3.179085432754991E-4 0.0 0.08992837917905683 1.390849876830309E-4 48-49 0.0011524184693736843 3.179085432754991E-4 0.0 0.11091034303523976 2.3843140745662437E-4 50-51 0.0011524184693736843 4.3712424700381134E-4 0.0 0.13588603296632118 2.3843140745662437E-4 52-53 0.0011524184693736843 5.563399507321235E-4 0.0 0.16702120092336536 2.3843140745662437E-4 54-55 0.0011524184693736843 5.563399507321235E-4 0.0 0.21027663209278796 2.3843140745662437E-4 56-57 0.0011921570372831218 5.960785186415609E-4 0.0 0.3103780846566608 2.3843140745662437E-4 58-59 0.0011921570372831218 5.960785186415609E-4 0.0 0.40100188877413273 2.3843140745662437E-4 60-61 0.0012120263212378405 5.960785186415609E-4 0.0 0.47088216044287834 2.3843140745662437E-4 62-63 0.0012318956051925593 5.960785186415609E-4 0.0 0.5592209969055577 2.781699753660618E-4 64-65 0.0012318956051925593 6.159478025962795E-4 0.0 0.649685846751392 2.781699753660618E-4 66-67 0.001311372741011434 6.358170865509982E-4 0.0 0.7942746260898799 2.781699753660618E-4 68-69 0.0013511113089208714 6.358170865509982E-4 0.0 0.9650312523967324 2.781699753660618E-4 70-71 0.0014305884447397463 6.358170865509982E-4 0.0 1.0556351872302496 2.781699753660618E-4 72-73 0.0014703270126491836 6.358170865509982E-4 0.0 1.174135596736192 2.781699753660618E-4 74-75 0.0014703270126491836 6.358170865509982E-4 0.0 1.3900749747560748 2.781699753660618E-4 76-77 0.0014901962966039023 6.358170865509982E-4 0.0 1.6411035082399907 2.781699753660618E-4 78-79 0.001510065580558621 6.358170865509982E-4 0.0 1.89260890453882 2.781699753660618E-4 80-81 0.00182797412383412 6.358170865509982E-4 0.0 2.111508805867956 2.9803925932078045E-4 82-83 0.0018677126917435575 6.358170865509982E-4 0.0 2.424350681735002 3.179085432754991E-4 84-85 0.0018875819756982762 6.358170865509982E-4 0.0 2.879794408545064 3.179085432754991E-4 86-87 0.001907451259652995 6.358170865509982E-4 0.0 3.2555821759806585 3.3777782723021785E-4 88-89 0.001907451259652995 6.358170865509982E-4 0.0 3.7692230354940914 3.5764711118493653E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3645 0.0 47.598007 1 GTATCAA 6610 0.0 42.93053 1 ATCAACG 8015 0.0 34.56437 3 TCAACGC 8120 0.0 34.120136 4 TATCAAC 8395 0.0 33.226227 2 CAACGCA 8365 0.0 33.12278 5 AACGCAG 8515 0.0 32.691097 6 ACGCAGA 10055 0.0 27.306808 7 CGCAGAG 10070 0.0 27.123533 8 TACATGG 7590 0.0 26.044449 2 GTACATG 7820 0.0 26.015394 1 ACATGGG 7630 0.0 25.534239 3 GCAGAGT 11345 0.0 23.070393 9 CATGGGG 5080 0.0 21.98378 4 GAGTACT 6595 0.0 21.35547 12-13 AGAGTAC 10065 0.0 20.081385 10-11 GTACTTT 6990 0.0 19.672413 14-15 CAGAGTA 10810 0.0 19.114874 10-11 CGTATAC 175 9.912916E-5 19.007439 3 CCGTCCG 275 2.84017E-8 19.000624 9 >>END_MODULE