FastQCFastQC Report
Thu 26 May 2016
SRR937393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61220.24327951274157575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.2036204219679572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.132647339681702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.043.483831
GTATCAA71400.039.6137351
ATCAACG87100.031.9059123
TCAACGC88350.031.4545024
TATCAAC90000.031.194532
CAACGCA90000.030.9306165
AACGCAG93000.029.9791726
ACGCAGA110650.025.0274227
CGCAGAG111000.024.7773328
GTACATG78200.024.4369131
TACATGG77200.023.9983182
ACATGGG76000.023.5021573
GCAGAGT124800.021.428559
GAGTACT69900.020.31856212-13
CATGGGG48000.020.1893544
GTACTTT70350.019.78189814-15
AGAGTAC107300.019.34553110-11
TAAGGTG8700.019.110955
CAGAGTA116900.018.9352210-11
AGTACTT74650.017.94395812-13
Produced by FastQC (version 0.11.5)