FastQCFastQC Report
Thu 26 May 2016
SRR937392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968424
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.2139182273152353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.16493600644651843No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41410.13950163453738415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.10746443230481897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.047.684051
GTATCAA74900.042.3782541
ATCAACG92600.034.062263
TCAACGC93250.033.776744
TATCAAC95150.033.3490942
CAACGCA95350.032.934865
AACGCAG98250.032.1067166
ACGCAGA114650.027.098767
CGCAGAG114800.027.0224278
GTACATG89250.024.9164431
TACATGG86950.024.5843852
ACATGGG88200.023.4280993
GCAGAGT128600.023.0140369
GAGTACT73150.021.33282912-13
TGGTATC23100.020.5638482
CATGGGG56050.020.4265254
AGAGTAC112050.020.18040310-11
GTACTTT78350.019.5515614-15
AGTACTT77600.019.49732812-13
CAGAGTA121650.019.32981910-11
Produced by FastQC (version 0.11.5)