FastQCFastQC Report
Thu 26 May 2016
SRR937392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968424
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68000.22907778673127557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.19222321339539095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.1289910066755962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.046.5453451
GTATCAA82250.040.6347121
ATCAACG102950.031.743293
TCAACGC104050.031.4077054
TATCAAC106050.031.1737042
CAACGCA106800.030.5989865
AACGCAG107850.030.4762256
ACGCAGA124650.026.2180637
CGCAGAG125400.025.7960998
GTACATG91750.024.6648581
TACATGG88200.024.3960462
ACATGGG88800.023.4288483
GCAGAGT144600.021.943849
AGAGTAC123850.021.171310-11
GAGTACT82650.020.83385512-13
AGTACTT87950.019.9024312-13
TAAGGTG10150.019.6555
GTACTTT85350.019.61691914-15
CCTATAC7050.018.8650863
CATGGGG57350.018.8010564
Produced by FastQC (version 0.11.5)