##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937392_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2968424 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25002492905326 33.0 31.0 34.0 31.0 34.0 2 32.45894723934317 34.0 31.0 34.0 31.0 34.0 3 32.52355020711327 34.0 31.0 34.0 31.0 34.0 4 36.142506596092744 37.0 35.0 37.0 35.0 37.0 5 36.05921121780447 37.0 35.0 37.0 35.0 37.0 6 36.091454926924186 37.0 35.0 37.0 35.0 37.0 7 36.03261629740226 37.0 35.0 37.0 35.0 37.0 8 36.004768186754994 37.0 35.0 37.0 35.0 37.0 9 37.73018645584324 39.0 37.0 39.0 35.0 39.0 10-11 37.645866796657074 39.0 37.0 39.0 35.0 39.0 12-13 37.552802092962466 39.0 37.0 39.0 35.0 39.0 14-15 38.80683200917389 40.0 38.0 41.0 35.0 41.0 16-17 38.76740536392375 40.0 38.0 41.0 35.0 41.0 18-19 38.724254014925094 40.0 38.0 41.0 34.5 41.0 20-21 38.60560368060628 40.0 38.0 41.0 34.0 41.0 22-23 38.458601096069835 40.0 38.0 41.0 34.0 41.0 24-25 38.40560108663722 40.0 38.0 41.0 34.0 41.0 26-27 38.36895857869361 40.0 38.0 41.0 34.0 41.0 28-29 38.25146576095598 40.0 38.0 41.0 34.0 41.0 30-31 38.37909257572368 40.0 38.0 41.0 34.0 41.0 32-33 38.36552140125535 40.0 38.0 41.0 34.0 41.0 34-35 38.26798614348894 40.0 38.0 41.0 34.0 41.0 36-37 38.133558581927645 40.0 38.0 41.0 33.5 41.0 38-39 38.02530938976373 40.0 38.0 41.0 33.0 41.0 40-41 37.878600058482206 40.0 37.0 41.0 33.0 41.0 42-43 37.70144477338817 40.0 37.0 41.0 33.0 41.0 44-45 37.47981875230762 40.0 37.0 41.0 32.5 41.0 46-47 37.22698408313637 40.0 37.0 41.0 32.0 41.0 48-49 36.981357110709254 40.0 36.5 41.0 31.0 41.0 50-51 36.77634040824357 39.0 36.0 41.0 31.0 41.0 52-53 36.55004911697251 39.0 36.0 41.0 30.5 41.0 54-55 36.32734659873387 39.0 35.0 40.0 30.0 41.0 56-57 36.05912632427173 39.0 35.0 40.0 29.5 41.0 58-59 35.79518862534463 38.0 35.0 40.0 29.0 41.0 60-61 35.47264828070384 38.0 34.5 40.0 28.0 41.0 62-63 35.10563197845052 38.0 34.0 40.0 28.0 41.0 64-65 34.71020312462101 37.0 34.0 40.0 27.5 41.0 66-67 34.29637056566043 36.5 33.5 39.0 26.5 41.0 68-69 33.85659983209946 36.0 33.0 39.0 26.0 40.0 70-71 33.4453460826351 35.5 33.0 38.5 26.0 40.0 72-73 32.96742918127599 35.0 32.0 38.0 25.5 40.0 74-75 32.488791695525975 35.0 32.0 37.0 24.5 39.0 76-77 31.224419591001826 34.0 30.5 36.0 23.0 38.5 78-79 31.83172232133954 34.5 31.0 36.0 24.0 38.5 80-81 31.646931839925834 34.5 31.0 36.0 24.0 37.5 82-83 31.311253042018258 34.0 31.0 35.0 23.0 37.0 84-85 30.963115444424382 34.0 31.0 35.0 21.5 36.5 86-87 30.55619463392022 34.0 31.0 35.0 20.0 36.0 88-89 30.110108259466976 34.0 30.0 35.0 18.0 36.0 90-91 29.631042600383232 34.0 30.0 35.0 14.5 35.0 92-93 29.28277311462244 34.0 29.0 35.0 7.0 35.0 94-95 28.86101261140592 34.0 29.0 35.0 2.0 35.0 96-97 28.38879789410138 34.0 29.0 35.0 2.0 35.0 98-99 27.651008919211 33.0 28.0 35.0 2.0 35.0 100-101 25.174247681598047 31.0 22.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13391040492306416 1101 2 -0.12435060739432657 1101 3 -0.11088722955798147 1101 4 -0.0634293753193802 1101 5 -0.10810432687501503 1101 6 -0.16273464412820715 1101 7 -0.10925057935393312 1101 8 -0.17327720213147302 1101 9 -0.1632803761814614 1101 10-11 -0.11487910699356263 1101 12-13 -0.2236124574123579 1101 14-15 -0.299705967074523 1101 16-17 -0.16223092105819603 1101 18-19 -0.14410794701753105 1101 20-21 -0.16894760150438515 1101 22-23 -0.1705042607660161 1101 24-25 -0.243872493839838 1101 26-27 -0.2544432069131233 1101 28-29 -0.3821756326983916 1101 30-31 -0.2792264396390749 1101 32-33 -0.18276589922792397 1101 34-35 -0.16967896981658726 1101 36-37 -0.2174433663033568 1101 38-39 -0.24487348936264652 1101 40-41 -0.21432278160449414 1101 42-43 -0.26917936037537515 1101 44-45 -0.2783335763347168 1101 46-47 -0.35952336652989914 1101 48-49 -0.3574308180409318 1101 50-51 -0.2767909091212246 1101 52-53 -0.27794767542794574 1101 54-55 -0.2818204377940319 1101 56-57 -0.2267575551503498 1101 58-59 -0.18509540935331614 1101 60-61 -0.29728329308131407 1101 62-63 -0.33551481831629104 1101 64-65 -0.3132221564454838 1101 66-67 -0.2485514950287424 1101 68-69 -0.2603279478039511 1101 70-71 -0.16643274986772383 1101 72-73 -0.10980165365443639 1101 74-75 -0.17101748007377893 1101 76-77 -0.11842163470812395 1101 78-79 -0.33612422939146924 1101 80-81 -0.35302663809874346 1101 82-83 -0.4402583043753232 1101 84-85 -0.4844245787681096 1101 86-87 -0.4780432436735893 1101 88-89 -0.4197808904345379 1101 90-91 -0.4928794865207564 1101 92-93 -0.5324865625699751 1101 94-95 -0.5224267123888495 1101 96-97 -0.5994491452910786 1101 98-99 -0.33292583552334065 1101 100-101 0.4370424551501735 1102 1 -0.129819242450111 1102 2 -0.14042221029736623 1102 3 -0.13686959668000043 1102 4 -0.08290213537012647 1102 5 -0.09625435606079691 1102 6 -0.11646498789525594 1102 7 -0.15369785139508707 1102 8 -0.1677759598387496 1102 9 -0.24108658848315656 1102 10-11 -0.16745193497458644 1102 12-13 -0.24023396936041053 1102 14-15 -0.2537595402544994 1102 16-17 -0.2669390168111079 1102 18-19 -0.3272383899052471 1102 20-21 -0.2979615524663899 1102 22-23 -0.23735343200082326 1102 24-25 -0.28952179649559184 1102 26-27 -0.41663593794343967 1102 28-29 -0.4418375929454754 1102 30-31 -0.3498317937406341 1102 32-33 -0.30722233245590047 1102 34-35 -0.3062580915975204 1102 36-37 -0.35871554358981683 1102 38-39 -0.3661225162201376 1102 40-41 -0.23234030145410856 1102 42-43 -0.3294872698013691 1102 44-45 -0.27828617916957654 1102 46-47 -0.2518552130532328 1102 48-49 -0.11661074855226872 1102 50-51 -0.2179349838187008 1102 52-53 -0.18686266881046265 1102 54-55 -0.16762090399640073 1102 56-57 -0.2799106161691327 1102 58-59 -0.2099565815276847 1102 60-61 -0.21109205792138397 1102 62-63 -0.26311624315322746 1102 64-65 -0.31141874244523393 1102 66-67 -0.3036975870220502 1102 68-69 -0.15926510176783637 1102 70-71 -0.18215796273592844 1102 72-73 -0.3223275692011853 1102 74-75 -0.3690515566535524 1102 76-77 -0.36881428935935645 1102 78-79 -0.35419512060087044 1102 80-81 -0.3279975089449252 1102 82-83 -0.2921803302060404 1102 84-85 -0.37687287961654903 1102 86-87 -0.4343008416600078 1102 88-89 -0.34083221958242405 1102 90-91 -0.32224771005688524 1102 92-93 -0.2216976313766139 1102 94-95 -0.18667719955469408 1102 96-97 -0.13932315717060817 1102 98-99 -0.06026226094126841 1102 100-101 0.5308605284084074 1103 1 0.020135817317367355 1103 2 0.02626759170541959 1103 3 -0.044004610122918564 1103 4 -0.02204304434506099 1103 5 0.01517329584868321 1103 6 -0.09080528978550007 1103 7 -0.09659944188523895 1103 8 -0.08240821156670108 1103 9 -0.08637308057087267 1103 10-11 -0.10915950845211597 1103 12-13 -0.011324238600145975 1103 14-15 -0.042008503079912884 1103 16-17 -0.1763364514033725 1103 18-19 -0.14660281148858445 1103 20-21 -0.21992027464808928 1103 22-23 -0.12722993730283605 1103 24-25 -0.1477313210461162 1103 26-27 -0.17375403248768606 1103 28-29 -0.26478630752598775 1103 30-31 -0.23906270948818786 1103 32-33 -0.18098003437560095 1103 34-35 -0.11041009290303094 1103 36-37 -0.10898082105849483 1103 38-39 -0.15130132100193805 1103 40-41 -0.17939712672350083 1103 42-43 -0.04195544976742838 1103 44-45 -0.1305228083136285 1103 46-47 -0.01846607914168885 1103 48-49 -0.12361437686345056 1103 50-51 -0.13610817347127835 1103 52-53 -0.007888129637308339 1103 54-55 -0.05800374475969505 1103 56-57 -0.2035053371190898 1103 58-59 -0.20759769297364983 1103 60-61 -0.12249604610808973 1103 62-63 -0.0637526943551805 1103 64-65 -0.22192774678541127 1103 66-67 -0.012270946836189012 1103 68-69 -0.09213152444196027 1103 70-71 0.009555326159038202 1103 72-73 0.1304656405545117 1103 74-75 0.12728539758091983 1103 76-77 0.20618872307613145 1103 78-79 0.09082591087219427 1103 80-81 -0.07202912903399294 1103 82-83 -0.16818084402522615 1103 84-85 -0.2018208219525981 1103 86-87 -0.17490213767669616 1103 88-89 -0.179986037190762 1103 90-91 -0.22212401705601792 1103 92-93 -0.09938757206510829 1103 94-95 -0.07970972046933866 1103 96-97 0.11043638479773321 1103 98-99 0.39213023805648106 1103 100-101 1.0479659833543735 1104 1 0.008066212688653707 1104 2 0.016820208444627838 1104 3 -0.03840748062885524 1104 4 -0.029034362808246783 1104 5 -0.01795348643790362 1104 6 0.01103580132466675 1104 7 -0.02418901327111911 1104 8 -0.011890657607388277 1104 9 -0.028259100187284503 1104 10-11 -0.02335698985543644 1104 12-13 -0.04085802903682634 1104 14-15 -0.030645927774017423 1104 16-17 -0.10302569606984946 1104 18-19 -0.02966288997524913 1104 20-21 -0.05583141054350449 1104 22-23 -0.13583357922735928 1104 24-25 -0.08974523370722665 1104 26-27 -0.1988994320852413 1104 28-29 -0.13911330322442694 1104 30-31 -0.1637352539795245 1104 32-33 -0.10625007299837108 1104 34-35 -0.02835264837236906 1104 36-37 -0.12656447689611383 1104 38-39 -0.18762622131339413 1104 40-41 -0.1635855436667768 1104 42-43 -0.09458190783993103 1104 44-45 -0.11197792087858716 1104 46-47 -0.10139113021791957 1104 48-49 -0.0077199170979938 1104 50-51 -0.1494600921513225 1104 52-53 -0.08738969304248911 1104 54-55 -0.0705590045978326 1104 56-57 -0.20537553442569134 1104 58-59 -0.28673245927061686 1104 60-61 -0.12624126415726522 1104 62-63 -0.025959705716097403 1104 64-65 -0.12129996579560753 1104 66-67 -0.1363329955164616 1104 68-69 -0.0761598321077841 1104 70-71 -0.0742391312522841 1104 72-73 -0.00781118190698038 1104 74-75 -0.031076572203780017 1104 76-77 0.13912392136578333 1104 78-79 -0.11339779202488032 1104 80-81 -0.19708278975912208 1104 82-83 -0.13633673574787153 1104 84-85 -0.03056026447714899 1104 86-87 -0.1453248643247349 1104 88-89 -0.012370341499750026 1104 90-91 -0.0721025239693347 1104 92-93 -0.006502989616638644 1104 94-95 0.04632090201379313 1104 96-97 0.010415564832335633 1104 98-99 0.11683878553777305 1104 100-101 0.7014302578559715 1105 1 0.012585485005637054 1105 2 0.014677467442972159 1105 3 0.008282533627642863 1105 4 -0.05482945199891276 1105 5 -0.032218513473139865 1105 6 -0.05714877309610955 1105 7 -0.13044435271848442 1105 8 -0.09642123989574003 1105 9 -0.09953347973339532 1105 10-11 -0.03337505077477232 1105 12-13 -0.06072380509939279 1105 14-15 -0.028724288178516133 1105 16-17 -0.017548835696345577 1105 18-19 -0.060342085646730936 1105 20-21 -0.0559498777773797 1105 22-23 -0.11662111346495152 1105 24-25 -0.058940384163982174 1105 26-27 0.023757319716132486 1105 28-29 -0.062360684368663044 1105 30-31 -0.10463863232816095 1105 32-33 -0.0943750522244855 1105 34-35 -0.07799367581005612 1105 36-37 -0.08986658428784011 1105 38-39 -0.07442971258208075 1105 40-41 -0.13414514470057526 1105 42-43 -0.015452083921537962 1105 44-45 0.045448975293361116 1105 46-47 0.0073531440529279735 1105 48-49 0.13187041759271523 1105 50-51 0.08548412606375422 1105 52-53 0.02074001139804693 1105 54-55 0.1504242711974868 1105 56-57 0.16279648583702766 1105 58-59 0.16761206545456275 1105 60-61 -0.003729076068580639 1105 62-63 0.12787813858000163 1105 64-65 0.0631762001690177 1105 66-67 0.05873762218404721 1105 68-69 0.2382807285577755 1105 70-71 0.19269191385554052 1105 72-73 0.29416898387079726 1105 74-75 0.2919603302834233 1105 76-77 0.4219752783377686 1105 78-79 0.18689429988862472 1105 80-81 0.10496037139134984 1105 82-83 0.1023895629449676 1105 84-85 0.12302020834972538 1105 86-87 0.1377063831767842 1105 88-89 0.18821186600183992 1105 90-91 0.4442741394004628 1105 92-93 0.5315429590510057 1105 94-95 0.6110412923919668 1105 96-97 0.42330273371922544 1105 98-99 0.5066563976515681 1105 100-101 1.161650195925116 1106 1 -0.11808888765958159 1106 2 0.0033991322515944944 1106 3 -0.05885646772188835 1106 4 0.01794529423121105 1106 5 -0.0617252513322768 1106 6 -0.040220024245165575 1106 7 -0.06009973420453463 1106 8 -0.10550903742747408 1106 9 -0.0675702103559388 1106 10-11 -0.14023759453662166 1106 12-13 -0.1491271574043438 1106 14-15 -0.12533482231765447 1106 16-17 0.004331603975856524 1106 18-19 -0.0023872068108445887 1106 20-21 0.05954915408321426 1106 22-23 -0.053427035739986195 1106 24-25 -0.014220166674888901 1106 26-27 -0.027780946238607385 1106 28-29 -0.08823787735111921 1106 30-31 0.05059812176443046 1106 32-33 -0.01026329568710338 1106 34-35 -0.021138955396978076 1106 36-37 -0.023940201999899102 1106 38-39 -0.05676165727101079 1106 40-41 -0.0115430011030071 1106 42-43 -0.16385612076951617 1106 44-45 -0.0029550615546156678 1106 46-47 -0.07203037606285534 1106 48-49 -0.014065841673065904 1106 50-51 0.09391525125689526 1106 52-53 0.10893848975671006 1106 54-55 0.05171316790971758 1106 56-57 -0.03652483865591449 1106 58-59 0.05665937715632907 1106 60-61 0.15976228891019417 1106 62-63 0.24453404814209279 1106 64-65 0.14732218385732665 1106 66-67 0.18589735540785313 1106 68-69 0.12182578126758159 1106 70-71 0.22352959892663193 1106 72-73 0.28937522690312534 1106 74-75 0.2542268522181459 1106 76-77 0.3475380704909483 1106 78-79 0.2964901261320456 1106 80-81 0.1741603361862829 1106 82-83 0.12932633072842137 1106 84-85 0.04516566396199906 1106 86-87 0.12965322352179598 1106 88-89 0.36074262154174974 1106 90-91 0.27295404708494786 1106 92-93 0.3194060113305319 1106 94-95 0.41089460460225524 1106 96-97 0.5283126106970641 1106 98-99 0.4759922931316325 1106 100-101 1.2404210500392203 1107 1 -0.015949187721531644 1107 2 -0.08411333533268817 1107 3 -0.08720381724470627 1107 4 -0.07912547965143091 1107 5 -0.036433641097836755 1107 6 -0.06731431592888981 1107 7 -0.056272317068035704 1107 8 -0.08824917622449391 1107 9 0.03138566226997597 1107 10-11 -0.043770514208638645 1107 12-13 -0.031361768468208595 1107 14-15 -0.10215902675454203 1107 16-17 -0.10092945411521725 1107 18-19 -0.08341495571632862 1107 20-21 -0.05640646656625847 1107 22-23 0.0031860334380482414 1107 24-25 -0.03632060028434836 1107 26-27 -0.1463471477987568 1107 28-29 -0.06430562751648239 1107 30-31 0.021625555699358756 1107 32-33 0.07503965348714559 1107 34-35 0.045015096699152934 1107 36-37 -0.01889542368667918 1107 38-39 -0.08348743341093723 1107 40-41 -0.04451989276364543 1107 42-43 0.012031308755830139 1107 44-45 0.064404540322613 1107 46-47 -0.06302983011390495 1107 48-49 0.1274816611354268 1107 50-51 0.14838179613494873 1107 52-53 0.11616181437585027 1107 54-55 0.19446044603921564 1107 56-57 0.14529374880615364 1107 58-59 0.1683303484205183 1107 60-61 0.19202367247083174 1107 62-63 0.19857459535092659 1107 64-65 0.14751352846661092 1107 66-67 0.20907892543105078 1107 68-69 0.32356100652402375 1107 70-71 0.3879563000300834 1107 72-73 0.3782591287582946 1107 74-75 0.46764118547798006 1107 76-77 0.5012628597231554 1107 78-79 0.4067159075214839 1107 80-81 0.5065133236819506 1107 82-83 0.3234833645457549 1107 84-85 0.3376984996418635 1107 86-87 0.4237924090079517 1107 88-89 0.48551846750015315 1107 90-91 0.5975826443111067 1107 92-93 0.69363748318791 1107 94-95 0.694283057781881 1107 96-97 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2316 62-63 0.41997264463331163 2316 64-65 0.6834332949684452 2316 66-67 0.694230688741186 2316 68-69 0.737469056121391 2316 70-71 0.6537927568213746 2316 72-73 0.68718224444698 2316 74-75 0.8893145715163868 2316 76-77 0.6229766669821757 2316 78-79 0.7206129331495923 2316 80-81 0.6982831432038239 2316 82-83 0.5825427049974259 2316 84-85 0.46548730138890093 2316 86-87 0.2948579018843702 2316 88-89 0.6850116273897022 2316 90-91 0.9437142810030785 2316 92-93 0.986218876827607 2316 94-95 0.7731753063566416 2316 96-97 0.8290436048491117 2316 98-99 1.1919279656462507 2316 100-101 0.9515321611503325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 17.0 5 54.0 6 88.0 7 266.0 8 578.0 9 1156.0 10 1983.0 11 3084.0 12 4641.0 13 6311.0 14 8266.0 15 11109.0 16 14168.0 17 19455.0 18 18052.0 19 12716.0 20 12015.0 21 12657.0 22 13421.0 23 14972.0 24 17602.0 25 21081.0 26 25863.0 27 32971.0 28 41072.0 29 51901.0 30 65376.0 31 82924.0 32 107259.0 33 141276.0 34 195140.0 35 281513.0 36 444038.0 37 690624.0 38 563992.0 39 50771.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77974615267922 19.257707283361707 14.477522881655908 23.485023682303165 2 15.976491229015801 20.260919599086925 39.280843976466976 24.481745195430303 3 19.555764270872356 23.57655105874363 29.521928134255752 27.345756536128263 4 13.289071911559805 16.38862911767322 36.20540731377997 34.11689165698701 5 13.954734064396982 37.38352610473967 34.41559994731113 14.246139883552214 6 33.587351402629814 35.826216200920086 16.71607560105969 13.870356795390414 7 29.451587778565326 32.14150000134752 20.192836333353995 18.214075886733163 8 26.679443367928567 35.799670127987106 19.12829838324983 18.392588120834493 9 26.20612823504998 16.50872651615807 19.632640081066587 37.652505167725366 10-11 25.197592456998596 26.730714196320903 27.122841928897877 20.94885141778262 12-13 25.667141242922625 23.584710514098823 27.875470561560938 22.87267768141761 14-15 22.687442898992867 25.405939313251746 25.490108555920582 26.416509231834805 16-17 22.52178260248536 28.609103685996338 26.305810760187896 22.563302951330403 18-19 22.52781273834196 27.390729895729184 28.250108475069602 21.831348890859257 20-21 23.055415937884884 26.60866506941057 28.430490388165573 21.905428604538972 22-23 22.788601328292007 26.36933686632708 28.321483789996527 22.52057801538439 24-25 22.614542439155528 26.586620406187357 28.547165930834655 22.251671223822463 26-27 22.50545408729774 26.84505810506344 28.27706763472505 22.37242017291377 28-29 22.500462801614844 27.0613369214285 28.265614943329332 22.172585333627325 30-31 22.615731757273913 26.801331080279855 28.263558746482975 22.31937841596326 32-33 22.611426130498877 26.67467652868997 28.2473460664649 22.46655127434625 34-35 22.755105065853126 26.79701417317742 28.262657221475102 22.18522353949436 36-37 22.865453665893696 26.844013328960642 28.135709072509364 22.154823932636297 38-39 22.552368927348674 26.99299389471337 28.210581351436847 22.244055826501114 40-41 22.859291185448424 26.81258824135795 28.03103063884806 22.297089934345564 42-43 22.560513062836552 26.825069932102057 28.07059836273387 22.543818642327516 44-45 22.693035805754544 27.02783417236007 27.96402260529551 22.31510741658987 46-47 22.918541960312947 27.139872875303528 27.60970130951643 22.331883854867094 48-49 22.863192724489494 27.184947298633887 27.555430086807004 22.396429890069612 50-51 22.905841618313286 27.39221216376097 27.459234260334775 22.242711957590963 52-53 23.019504626023775 27.348198909589733 27.2238736784233 22.408422785963193 54-55 22.9603318124365 27.322174999258863 27.249644929430566 22.467848258874067 56-57 22.861087230126152 27.543454034868336 27.219174215004326 22.376284520001185 58-59 22.932775102209117 27.249409114061873 27.36175829328964 22.45605749043937 60-61 22.84528760042366 27.34349944617076 27.49031135713766 22.32090159626792 62-63 22.65929665034375 27.339507428857875 27.664730510196655 22.33646541060172 64-65 22.72381282522524 27.279480168513693 27.516272526477437 22.48043447978363 66-67 22.65929665034375 27.45539383861605 27.30851455182952 22.576794959210677 68-69 22.64752272586396 27.292226447434732 27.452378770687748 22.607872056013562 70-71 22.750809857351914 27.243075787016952 27.396355776668024 22.609758578963113 72-73 22.672754970314212 27.24605716703544 27.298829277758163 22.782358584892183 74-75 22.595879160119985 27.60447968349535 27.036821559184265 22.7628195972004 76-77 22.78859084820767 27.366440912753703 27.205362171980823 22.639606067057805 78-79 22.754363931837233 27.382863768787747 27.14169202243345 22.721080276941567 80-81 22.74981454793153 27.372236831555618 27.0729532391284 22.80499538138445 82-83 22.6603683580738 27.379703518644288 27.12251247803542 22.83741564524649 84-85 22.692076411148708 27.32594667524588 27.134716675397474 22.847260238207934 86-87 22.68737552317324 27.30236650828857 27.17721592333171 22.83304204520648 88-89 22.604250605708618 27.337637749863227 26.97781718514606 23.080294459282097 90-91 22.657118980145636 27.163571316309593 27.148797995814615 23.030511707730152 92-93 22.411201389595416 27.110350343255135 27.29028219756053 23.188166069588924 94-95 22.409635550716477 27.196738067068587 27.21105542873929 23.182570953475647 96-97 22.51332693712219 27.207871921261923 27.02727103675216 23.251530104863726 98-99 22.212362877343125 27.20510480494836 26.989215471266014 23.593316846442498 100-101 22.10297356475696 27.08080297265509 27.306619238231868 23.50960422435608 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 152.0 2 142.0 3 183.0 4 173.5 5 193.0 6 345.0 7 719.5 8 1129.5 9 1408.0 10 1456.5 11 1479.5 12 1655.5 13 1812.5 14 2037.0 15 2242.5 16 2417.0 17 2678.5 18 2907.5 19 3204.0 20 3606.5 21 4276.5 22 5184.0 23 6311.0 24 7741.5 25 9801.5 26 13058.0 27 17436.0 28 21312.0 29 25381.0 30 30772.0 31 35981.0 32 41933.5 33 47183.5 34 53309.0 35 60833.0 36 68564.5 37 75679.5 38 80889.0 39 85003.0 40 88611.0 41 93748.0 42 98143.5 43 103400.0 44 110273.0 45 113796.5 46 115521.0 47 119564.5 48 121702.0 49 120330.5 50 118096.5 51 114032.5 52 110959.5 53 109330.5 54 107680.5 55 100112.5 56 86480.0 57 75800.5 58 69674.5 59 61948.5 60 50194.5 61 39930.0 62 31701.5 63 23798.0 64 16774.0 65 11709.0 66 8663.0 67 6871.0 68 5305.0 69 4163.5 70 3239.5 71 2055.0 72 1372.5 73 998.5 74 668.0 75 409.0 76 262.5 77 150.0 78 82.0 79 59.5 80 43.5 81 25.5 82 18.5 83 15.0 84 8.0 85 3.0 86 1.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06986872495303906 2 0.0 3 0.0 4 0.0 5 0.0018528350397382584 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1790768434698007E-4 12-13 0.0054574413897745064 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.590417002422834E-4 24-25 5.221626021080547E-4 26-27 2.6950327850738305E-4 28-29 0.003015067928301348 30-31 2.6950327850738305E-4 32-33 0.0 34-35 0.0 36-37 1.684395490671144E-5 38-39 4.379428275744974E-4 40-41 1.0106372944026864E-4 42-43 0.012346618946619484 44-45 1.5159559416040298E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.684395490671144E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.737581962684576E-5 82-83 2.6950327850738305E-4 84-85 3.0319118832080596E-4 86-87 0.0 88-89 0.0 90-91 0.007933502761061088 92-93 0.0026276569654469844 94-95 0.0 96-97 0.0 98-99 1.684395490671144E-4 100-101 0.010207436673467133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2968424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67273582522704 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27185403622045 37.86157157038215 2 14.138552881395258 14.611554159826797 3 5.104082534823639 7.912257253562912 4 2.4169791229614237 4.995676948634984 5 1.3338319004612638 3.446137171389771 6 0.8358192153699932 2.5913439308077324 7 0.5443256429001804 1.9688756603934525 8 0.3840275357903254 1.5875002725205125 9 0.29404839029495394 1.3674856312389971 >10 1.5208159504515084 14.285732894570598 >50 0.10759119142153672 3.823117903258945 >100 0.04565948745243901 4.2180593157994855 >500 0.00208614958455778 0.6923602811199051 >1k 1.9557652350773788E-4 0.21704263564511433 >5k 1.3038434900515859E-4 0.42128437084864195 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6800 0.22907778673127557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5706 0.19222321339539095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3829 0.1289910066755962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.368790981342288E-5 3 0.0 0.0 0.0 0.0 3.368790981342288E-5 4 0.0 0.0 0.0 0.0 3.368790981342288E-5 5 0.0 0.0 0.0 0.0 3.368790981342288E-5 6 3.368790981342288E-5 0.0 0.0 0.0 3.368790981342288E-5 7 6.737581962684576E-5 0.0 0.0 0.0 3.368790981342288E-5 8 6.737581962684576E-5 0.0 0.0 0.0 6.737581962684576E-5 9 6.737581962684576E-5 0.0 0.0 3.368790981342288E-5 6.737581962684576E-5 10-11 6.737581962684576E-5 0.0 0.0 5.053186472013432E-5 6.737581962684576E-5 12-13 6.737581962684576E-5 0.0 0.0 6.737581962684576E-5 6.737581962684576E-5 14-15 6.737581962684576E-5 0.0 0.0 6.737581962684576E-5 1.3475163925369152E-4 16-17 8.42197745335572E-5 0.0 0.0 6.737581962684576E-5 1.684395490671144E-4 18-19 1.3475163925369152E-4 0.0 0.0 6.737581962684576E-5 1.684395490671144E-4 20-21 1.684395490671144E-4 0.0 0.0 8.42197745335572E-5 1.684395490671144E-4 22-23 2.0212745888053727E-4 0.0 0.0 2.1897141378724873E-4 2.0212745888053727E-4 24-25 2.189714137872487E-4 0.0 0.0 4.379428275744974E-4 2.0212745888053727E-4 26-27 2.3581536869396016E-4 3.368790981342288E-5 0.0 7.074461060818805E-4 2.0212745888053727E-4 28-29 3.5372305304094025E-4 6.737581962684576E-5 0.0 0.0017517713102979896 2.0212745888053727E-4 30-31 4.0425491776107454E-4 8.42197745335572E-5 0.0 0.003604606350036248 2.0212745888053727E-4 32-33 4.0425491776107454E-4 1.0106372944026864E-4 0.0 0.005828008397722158 2.0212745888053727E-4 34-35 4.0425491776107454E-4 1.3475163925369152E-4 0.0 0.008927296100557064 2.0212745888053727E-4 36-37 4.0425491776107454E-4 2.3581536869396016E-4 0.0 0.012936157368354385 2.0212745888053727E-4 38-39 5.053186472013432E-4 2.3581536869396016E-4 0.0 0.019252640458371176 2.0212745888053727E-4 40-41 5.221626021080547E-4 2.3581536869396016E-4 0.0 0.032929931842620865 2.3581536869396016E-4 42-43 6.90602151175169E-4 2.3581536869396016E-4 0.0 0.04574818152662827 2.6950327850738305E-4 44-45 8.085098355221491E-4 2.3581536869396016E-4 0.0 0.06161518704875045 3.031911883208059E-4 46-47 0.0010443252042161093 2.3581536869396016E-4 0.0 0.09048572575885386 3.031911883208059E-4 48-49 0.001111701023842955 2.3581536869396016E-4 0.0 0.11194492431000423 3.200351432275174E-4 50-51 0.0011285449787496664 2.3581536869396016E-4 0.0 0.1381035862801271 3.368790981342288E-4 52-53 0.0011622328885630894 2.3581536869396016E-4 0.0 0.1693154347222634 3.5372305304094025E-4 54-55 0.0012127647532832236 2.3581536869396016E-4 0.0 0.21447407782715677 3.874109628543631E-4 56-57 0.0012464526630966465 2.3581536869396016E-4 0.0 0.30426920143483543 4.21098872667786E-4 58-59 0.0012464526630966465 2.3581536869396016E-4 0.0 0.3935926943051262 4.3794282757449746E-4 60-61 0.0012464526630966465 2.526593236006716E-4 0.0 0.46327613575419146 4.3794282757449746E-4 62-63 0.0012464526630966465 3.031911883208059E-4 0.0 0.5474453784230285 4.3794282757449746E-4 64-65 0.0012464526630966465 3.368790981342288E-4 0.0 0.639935534815781 4.3794282757449746E-4 66-67 0.0013138284827234924 3.368790981342288E-4 0.0 0.7768768882073451 4.3794282757449746E-4 68-69 0.0013138284827234924 3.368790981342288E-4 0.0 0.9407685694496474 4.3794282757449746E-4 70-71 0.001330672437630204 3.368790981342288E-4 0.0 1.0393057056539092 4.716307373879203E-4 72-73 0.0013643603474436266 3.368790981342288E-4 0.0 1.1594199480936687 4.716307373879203E-4 74-75 0.0013812043023503382 3.368790981342288E-4 0.0 1.3638887167062386 4.716307373879203E-4 76-77 0.0013812043023503382 3.368790981342288E-4 0.0 1.6027191533285001 4.716307373879203E-4 78-79 0.0013812043023503382 3.368790981342288E-4 0.0 1.8434024249904999 4.716307373879203E-4 80-81 0.0016001757161375869 3.368790981342288E-4 0.0 2.0604367839634765 4.716307373879203E-4 82-83 0.0016170196710442982 3.368790981342288E-4 0.0 2.350843410510089 5.053186472013432E-4 84-85 0.0017349273553912783 3.368790981342288E-4 0.0 2.7519821966134215 5.053186472013432E-4 86-87 0.0018696789946449697 3.368790981342288E-4 0.0 3.102100643304326 5.053186472013432E-4 88-89 0.0018865229495516813 3.368790981342288E-4 0.0 3.5527943447432038 5.053186472013432E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 46.545345 1 GTATCAA 8225 0.0 40.634712 1 ATCAACG 10295 0.0 31.74329 3 TCAACGC 10405 0.0 31.407705 4 TATCAAC 10605 0.0 31.173704 2 CAACGCA 10680 0.0 30.598986 5 AACGCAG 10785 0.0 30.476225 6 ACGCAGA 12465 0.0 26.218063 7 CGCAGAG 12540 0.0 25.796099 8 GTACATG 9175 0.0 24.664858 1 TACATGG 8820 0.0 24.396046 2 ACATGGG 8880 0.0 23.428848 3 GCAGAGT 14460 0.0 21.94384 9 AGAGTAC 12385 0.0 21.1713 10-11 GAGTACT 8265 0.0 20.833855 12-13 AGTACTT 8795 0.0 19.90243 12-13 TAAGGTG 1015 0.0 19.655 5 GTACTTT 8535 0.0 19.616919 14-15 CCTATAC 705 0.0 18.865086 3 CATGGGG 5735 0.0 18.801056 4 >>END_MODULE