FastQCFastQC Report
Thu 26 May 2016
SRR937391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976213
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97751.0013183598251612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74660.7647921099186346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53050.5434264858181566No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22670.23222391015075605No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19830.20313189846887922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.1799812131163998No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16160.16553764393631307No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15230.15601103447710696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14580.14935265152174781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.14730391830471423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.10714874725085613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113050.029.4232161
TACCTGG10100.029.1622772
GGTATCA78250.027.9341931
GTACCTG11500.026.4450761
CCATGCG1301.0008729E-525.580319
GTACATG81200.024.6955721
TACATGG80850.024.38482
TATCAAC136050.024.3031082
ATCAACG137800.023.9599953
TCAACGC138350.023.8684234
GAGTACT73050.023.86266312-13
ATGGGAG18350.023.8227635
CAACGCA139650.023.6134195
ACCTGGG11950.023.454993
ACATGGG81050.023.2695853
AACGCAG142000.023.2501246
AGTACTT75000.022.45060512-13
GTACTTT77600.022.43116814-15
ACGGTTC550.002880582621.5876792-93
ACGCAGA154050.021.3400547
Produced by FastQC (version 0.11.5)