##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937391_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976213 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.734894946082463 33.0 31.0 34.0 30.0 34.0 2 31.811734734120524 33.0 31.0 34.0 30.0 34.0 3 31.826520441747856 33.0 31.0 34.0 30.0 34.0 4 35.48097085369689 37.0 35.0 37.0 33.0 37.0 5 35.50027913990082 37.0 35.0 37.0 33.0 37.0 6 35.560367460789806 37.0 35.0 37.0 33.0 37.0 7 35.503135074005364 37.0 35.0 37.0 33.0 37.0 8 35.49621650193144 37.0 35.0 37.0 33.0 37.0 9 37.07535548082232 39.0 37.0 39.0 33.0 39.0 10-11 36.96063051813488 39.0 37.0 39.0 33.0 39.0 12-13 36.80824727800183 39.0 37.0 39.0 33.0 39.0 14-15 37.83005604309715 40.0 37.5 41.0 33.0 41.0 16-17 37.77287231372661 40.0 37.5 41.0 33.0 41.0 18-19 37.78400256911145 40.0 37.5 41.0 33.0 41.0 20-21 37.70642779803178 40.0 37.0 41.0 32.0 41.0 22-23 37.76555321430876 40.0 37.5 41.0 33.0 41.0 24-25 37.725398043254906 40.0 37.0 41.0 32.5 41.0 26-27 37.61844392565967 40.0 37.0 41.0 32.0 41.0 28-29 37.474506076030536 40.0 37.0 41.0 32.0 41.0 30-31 37.155115225877964 39.0 36.5 41.0 31.5 41.0 32-33 36.83971838113199 39.0 35.5 41.0 31.0 41.0 34-35 36.767453414367566 39.0 35.0 41.0 31.0 41.0 36-37 36.58612413479435 39.0 35.0 41.0 30.5 41.0 38-39 36.406399525513386 39.0 35.0 41.0 30.0 41.0 40-41 36.12188426091437 39.0 35.0 40.5 30.0 41.0 42-43 35.77779798056367 39.0 35.0 40.0 29.0 41.0 44-45 35.41036075118852 38.5 35.0 40.0 27.5 41.0 46-47 34.817519844542126 38.0 34.5 40.0 24.5 41.0 48-49 34.32568558296191 38.0 34.0 40.0 22.5 41.0 50-51 33.317583867455156 37.5 33.0 39.5 19.0 40.5 52-53 33.39642782876278 37.5 33.0 39.5 19.0 40.0 54-55 33.687975370129266 38.0 33.0 40.0 18.0 41.0 56-57 33.41738739393964 38.0 33.0 40.0 14.0 41.0 58-59 33.14111213433954 38.0 33.0 40.0 11.0 41.0 60-61 32.78878687335653 37.0 32.5 40.0 8.0 41.0 62-63 32.396684432598214 37.0 32.0 40.0 7.0 41.0 64-65 31.948540431237852 36.0 31.0 39.0 2.0 41.0 66-67 31.4988813916635 35.5 31.0 39.0 2.0 40.0 68-69 30.994761389164047 35.0 30.0 38.5 2.0 40.0 70-71 30.505755915973257 35.0 29.5 38.0 2.0 40.0 72-73 29.9881526879892 35.0 29.0 37.5 2.0 39.5 74-75 29.427840030812945 34.0 28.5 37.0 2.0 39.0 76-77 28.87098922059018 34.0 28.0 36.0 2.0 39.0 78-79 28.305535779589086 34.0 26.5 36.0 2.0 38.5 80-81 27.683650494308107 33.5 26.0 35.0 2.0 37.0 82-83 27.09949980178506 33.0 25.5 35.0 2.0 37.0 84-85 26.490131764276853 33.0 24.5 35.0 2.0 36.0 86-87 25.850753370422233 32.0 22.0 35.0 2.0 36.0 88-89 25.230974182888367 32.0 19.5 35.0 2.0 35.0 90-91 24.614083709190517 31.0 16.5 34.0 2.0 35.0 92-93 23.90490599899817 31.0 4.5 34.0 2.0 35.0 94-95 23.108040970566876 31.0 2.0 34.0 2.0 35.0 96-97 22.20215106744122 30.0 2.0 34.0 2.0 35.0 98-99 20.99095176974697 29.5 2.0 34.0 2.0 35.0 100-101 17.994187231679973 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23838827317043965 1101 2 0.11331219218752508 1101 3 0.25767111281161803 1101 4 0.02847031045365611 1101 5 0.01458962297726174 1101 6 0.07848679574050976 1101 7 0.050944550555072965 1101 8 -0.005928123765002624 1101 9 0.0928713821927829 1101 10-11 -0.0046595770701216566 1101 12-13 0.12136619103110036 1101 14-15 0.16543942716841542 1101 16-17 0.2807021102486118 1101 18-19 0.2587850139706589 1101 20-21 0.3078138780946986 1101 22-23 0.30969170559551884 1101 24-25 0.1679588256949316 1101 26-27 0.31875210140556476 1101 28-29 0.22675565654518692 1101 30-31 0.2430249328063212 1101 32-33 0.29015947135120257 1101 34-35 0.42404733511936143 1101 36-37 0.4404683687673412 1101 38-39 0.49721147732791593 1101 40-41 0.6512034776007312 1101 42-43 0.7952674054594553 1101 44-45 0.6920728025182257 1101 46-47 0.765366688472362 1101 48-49 0.606474024920125 1101 50-51 0.5811041409136948 1101 52-53 0.7148064277288526 1101 54-55 0.6532282287652933 1101 56-57 0.8955193474391407 1101 58-59 0.8046398036965599 1101 60-61 1.110274715611169 1101 62-63 0.9272334374347153 1101 64-65 0.9412906199832101 1101 66-67 1.2944435345554517 1101 68-69 1.2852434162375843 1101 70-71 1.2602164268908993 1101 72-73 1.2730802830193078 1101 74-75 1.4159766624945824 1101 76-77 1.494964986090043 1101 78-79 1.3511815263850835 1101 80-81 1.4609386139784597 1101 82-83 1.3988082108227786 1101 84-85 1.6093381241479747 1101 86-87 1.8048128457498684 1101 88-89 1.7181679791327902 1101 90-91 1.874581900345433 1101 92-93 2.056935870762775 1101 94-95 2.2899163929780286 1101 96-97 2.2154819877462195 1101 98-99 2.6062350396020726 1101 100-101 2.439117186247824 1102 1 0.20676947797936762 1102 2 0.16219600914393695 1102 3 0.14660039309826445 1102 4 -0.023162166684301155 1102 5 0.16825537240130473 1102 6 0.03500059886158624 1102 7 0.007889365680064486 1102 8 0.02757884145033529 1102 9 0.14216473724543732 1102 10-11 0.14015892239456207 1102 12-13 0.30403168583594464 1102 14-15 0.4157285579966654 1102 16-17 0.4852203738070813 1102 18-19 0.5022937584526304 1102 20-21 0.44638193657735314 1102 22-23 0.36034328530820403 1102 24-25 0.42165463858970753 1102 26-27 0.4954217744193059 1102 28-29 0.559694023362475 1102 30-31 0.8165347203066631 1102 32-33 0.5614378130746971 1102 34-35 0.650776521534354 1102 36-37 0.809654691728781 1102 38-39 0.678889939305293 1102 40-41 0.7207689684175662 1102 42-43 0.880963635561578 1102 44-45 0.9545575790515457 1102 46-47 0.9423017176308619 1102 48-49 1.0125272127217215 1102 50-51 1.0245380334889376 1102 52-53 1.1825104375209605 1102 54-55 1.2231664452652282 1102 56-57 1.199704918813815 1102 58-59 1.3127485046171756 1102 60-61 1.4185779087812378 1102 62-63 1.4636301770819955 1102 64-65 1.4568937146800671 1102 66-67 1.557126177809426 1102 68-69 1.4688483945029276 1102 70-71 1.728635977963524 1102 72-73 1.7404253717832425 1102 74-75 1.5763859858517506 1102 76-77 1.4958576871088276 1102 78-79 1.588306384887371 1102 80-81 1.8692948783774774 1102 82-83 1.7545196567223371 1102 84-85 1.6041560100724404 1102 86-87 2.0437530163754047 1102 88-89 1.9703268737787312 1102 90-91 2.0830206859039215 1102 92-93 2.3442751615716375 1102 94-95 2.262482709006459 1102 96-97 1.9266893735433754 1102 98-99 1.9717600613060569 1102 100-101 1.4675754823852856 1103 1 0.30631500454313354 1103 2 0.3118161694187904 1103 3 0.36623614723230347 1103 4 0.17504162087229957 1103 5 0.19259930455550744 1103 6 0.19700828738467635 1103 7 0.2743408871237065 1103 8 0.2849692466405003 1103 9 0.43943539493938033 1103 10-11 0.2604843086969879 1103 12-13 0.24888571230381729 1103 14-15 0.2683378192480106 1103 16-17 0.33073859860882493 1103 18-19 0.34095193605583773 1103 20-21 0.23840571929736143 1103 22-23 0.18523842285937775 1103 24-25 0.35095524983562854 1103 26-27 0.24648082261016668 1103 28-29 0.06587361125097146 1103 30-31 0.24463285321303374 1103 32-33 0.20109624918573843 1103 34-35 0.2827218346206877 1103 36-37 0.09988601453834178 1103 38-39 0.1884968406303571 1103 40-41 0.4755655394363387 1103 42-43 0.409467458975719 1103 44-45 0.4312418409283154 1103 46-47 0.2633063123989956 1103 48-49 0.3372568218745684 1103 50-51 0.22259505531199864 1103 52-53 0.282955738098849 1103 54-55 0.33844801032163474 1103 56-57 0.26502359225171546 1103 58-59 0.4558025661086802 1103 60-61 0.3827041106341582 1103 62-63 0.4374562596877567 1103 64-65 0.36002374411660654 1103 66-67 0.6948132832722784 1103 68-69 0.7009930087842271 1103 70-71 0.7860368068563552 1103 72-73 0.9583779064107816 1103 74-75 1.051459160244466 1103 76-77 0.9624380455174197 1103 78-79 1.0939556147842175 1103 80-81 1.2637482177148662 1103 82-83 0.9679658162133293 1103 84-85 1.293106885246516 1103 86-87 1.2397699111126848 1103 88-89 1.13558508974117 1103 90-91 1.348519480923894 1103 92-93 1.545597720965894 1103 94-95 1.5460695467886296 1103 96-97 1.6337541422622621 1103 98-99 1.9491502163273644 1103 100-101 1.7085302101598643 1104 1 0.21426968195980933 1104 2 0.22161394310447946 1104 3 0.15570262809628943 1104 4 0.3110566509822874 1104 5 0.24870839727436334 1104 6 0.2412766063680678 1104 7 0.2640495750581451 1104 8 0.279378576589572 1104 9 0.31341887177210737 1104 10-11 0.3177152680692359 1104 12-13 0.2545020961190687 1104 14-15 0.4512572439232656 1104 16-17 0.3965500752424802 1104 18-19 0.4629027812921649 1104 20-21 0.6192294721797893 1104 22-23 0.4675946331482024 1104 24-25 0.2836610843717011 1104 26-27 0.4613421341780466 1104 28-29 0.5062417328190136 1104 30-31 0.6163153628764348 1104 32-33 0.6885678396235377 1104 34-35 0.6263365263043639 1104 36-37 0.7086137638374339 1104 38-39 0.9173422926119699 1104 40-41 0.7949645009320463 1104 42-43 0.8105291885996309 1104 44-45 0.7990876081109093 1104 46-47 0.8364507739859448 1104 48-49 0.6948025674907896 1104 50-51 0.6467780211873517 1104 52-53 0.7147926962434781 1104 54-55 1.019629267475871 1104 56-57 1.1998296607000967 1104 58-59 1.2969761091336096 1104 60-61 1.1478432967520433 1104 62-63 1.219071747668643 1104 64-65 1.2143084037824998 1104 66-67 1.323493254738299 1104 68-69 1.0452704520065765 1104 70-71 1.1098589505548269 1104 72-73 1.0871536823858285 1104 74-75 1.0793805756627712 1104 76-77 1.1572368145746346 1104 78-79 1.2236160271998209 1104 80-81 1.226785510967904 1104 82-83 1.4930222205995989 1104 84-85 1.6098706006366186 1104 86-87 1.6941462279062627 1104 88-89 1.677040654697457 1104 90-91 1.9589252607145333 1104 92-93 2.036732278606202 1104 94-95 1.7683243950774177 1104 96-97 2.074423778453969 1104 98-99 1.9220508118913244 1104 100-101 1.8464754315470806 1105 1 -0.015644564733470645 1105 2 0.05584434666220872 1105 3 -0.00347907662618141 1105 4 0.05338406819426922 1105 5 0.15176287493483898 1105 6 0.039611555253642905 1105 7 -0.08375533849174133 1105 8 0.04650368846527897 1105 9 0.18261101306531913 1105 10-11 0.09084644058853542 1105 12-13 0.07754725209470337 1105 14-15 0.2284333618286425 1105 16-17 0.24162971216131268 1105 18-19 0.22273506838767076 1105 20-21 0.22763393110398056 1105 22-23 0.24642682114274095 1105 24-25 0.133072904639711 1105 26-27 0.11334869652029766 1105 28-29 0.11357659808815157 1105 30-31 0.12317733071198234 1105 32-33 0.10112052572431196 1105 34-35 0.035162861147945534 1105 36-37 0.11973722815953636 1105 38-39 0.12264117493886317 1105 40-41 5.440402983580839E-4 1105 42-43 0.09912359620810918 1105 44-45 0.08187570546522238 1105 46-47 0.3440912263818703 1105 48-49 0.12391130912526904 1105 50-51 0.22189926502837665 1105 52-53 0.2003533754567357 1105 54-55 0.38967809018733846 1105 56-57 0.16802483076664743 1105 58-59 0.3520685053021211 1105 60-61 0.3285875914783247 1105 62-63 0.2727008561005917 1105 64-65 0.3131457481242954 1105 66-67 0.15600332742266332 1105 68-69 0.21251572380646877 1105 70-71 0.42527139639769374 1105 72-73 0.5364298582033378 1105 74-75 0.5077836100105451 1105 76-77 0.46054036084085226 1105 78-79 0.5095827106930031 1105 80-81 0.45404377428537046 1105 82-83 0.6536977949981022 1105 84-85 0.9303368087548449 1105 86-87 0.7325021738095181 1105 88-89 0.6784776187392936 1105 90-91 0.7789540309644245 1105 92-93 0.8111056972489514 1105 94-95 0.8722828593840397 1105 96-97 0.9905266793251286 1105 98-99 1.0123585049136885 1105 100-101 0.8190538838996417 1106 1 0.08217381594814555 1106 2 -0.6072952470474888 1106 3 -0.060908018575549505 1106 4 0.12323525421708581 1106 5 0.0625065529237645 1106 6 -0.12279590361498549 1106 7 0.1386114699703782 1106 8 0.19233212308616743 1106 9 0.12182689843188399 1106 10-11 -0.15000320991059368 1106 12-13 -0.01971757408117014 1106 14-15 0.14014876623071615 1106 16-17 0.04064355681333609 1106 18-19 0.31493996995026663 1106 20-21 0.31253975113063603 1106 22-23 0.3415406672198529 1106 24-25 0.358415458240529 1106 26-27 0.2954044456492184 1106 28-29 0.4013496790398179 1106 30-31 0.4163078217232865 1106 32-33 0.41807924471991953 1106 34-35 0.24301612066214062 1106 36-37 0.10178255639108613 1106 38-39 0.2954575070680008 1106 40-41 0.4745822003775899 1106 42-43 0.32861179654585015 1106 44-45 0.4741824111790791 1106 46-47 0.3716553906080833 1106 48-49 0.39977829215968796 1106 50-51 0.5830840052719282 1106 52-53 0.6399665018684573 1106 54-55 0.7711644307957002 1106 56-57 0.49458101810766664 1106 58-59 0.32127119086534606 1106 60-61 0.37351769130517454 1106 62-63 0.5309235676002757 1106 64-65 0.5377089611096189 1106 66-67 0.6399667242433082 1106 68-69 0.8160050321438987 1106 70-71 0.8752644491327786 1106 72-73 1.0863780209189677 1106 74-75 0.7709384342625576 1106 76-77 0.9869270527314349 1106 78-79 1.103006055640737 1106 80-81 1.0877104872671381 1106 82-83 1.1102350822158442 1106 84-85 1.0288534786279406 1106 86-87 1.0805405512134527 1106 88-89 1.335164272152717 1106 90-91 1.3197407107418293 1106 92-93 1.4919162827430625 1106 94-95 1.5058743103292223 1106 96-97 1.4948771813095547 1106 98-99 1.3056800457101652 1106 100-101 0.987918736439493 1107 1 0.2070266651553716 1107 2 0.2618214803188792 1107 3 0.25604559213703126 1107 4 0.2993039368496113 1107 5 0.12187086149897652 1107 6 0.22073314027274193 1107 7 0.24191040680589992 1107 8 0.14556024360266662 1107 9 0.43536936982944496 1107 10-11 0.2775089300298106 1107 12-13 0.32890610616016147 1107 14-15 0.4202401836607308 1107 16-17 0.5275839239545661 1107 18-19 0.5313137145700679 1107 20-21 0.36758380439921723 1107 22-23 0.3160628869895632 1107 24-25 0.4827229298220317 1107 26-27 0.45743402295856583 1107 28-29 0.4072306524423155 1107 30-31 0.31089589147276797 1107 32-33 0.5010856273855637 1107 34-35 0.45175503836805575 1107 36-37 0.38076252548941625 1107 38-39 0.6270815919740116 1107 40-41 0.392974669935775 1107 42-43 0.4753629829491217 1107 44-45 0.5597358820984013 1107 46-47 0.7575790377404843 1107 48-49 0.5917806465723814 1107 50-51 0.29959248483636003 1107 52-53 0.386671243802752 1107 54-55 0.41856400037963226 1107 56-57 0.4241445320685955 1107 58-59 0.599617491862297 1107 60-61 0.6743702462108203 1107 62-63 0.7886894506889703 1107 64-65 0.38324937239609014 1107 66-67 0.5070389752825584 1107 68-69 0.8253785376436582 1107 70-71 0.6739704418488124 1107 72-73 1.0356854925004733 1107 74-75 0.8998117190361263 1107 76-77 1.0968675486296071 1107 78-79 1.2572233053924222 1107 80-81 1.4422560672251983 1107 82-83 1.5260606901325602 1107 84-85 1.5927160029997935 1107 86-87 1.430985682361598 1107 88-89 1.3784716701588238 1107 90-91 1.578872337125965 1107 92-93 1.151048828819654 1107 94-95 1.0562431070036133 1107 96-97 1.2144054710494672 1107 98-99 1.5938849899768002 1107 100-101 1.6879812755264219 1108 1 0.12019375728557335 1108 2 0.2783521082154081 1108 3 0.20206467773737202 1108 4 0.313659770447714 1108 5 0.13842340168063316 1108 6 0.35676570192843116 1108 7 0.25405419522087413 1108 8 0.24202798324089514 1108 9 0.3068330009142173 1108 10-11 0.3929565432341491 1108 12-13 0.2548486083239965 1108 14-15 0.553230224527141 1108 16-17 0.550884900146869 1108 18-19 0.33404836775337543 1108 20-21 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623.0 5 671.0 6 846.0 7 1024.0 8 1271.0 9 1668.0 10 2269.0 11 3137.0 12 4532.0 13 6367.0 14 7107.0 15 9597.0 16 12368.0 17 17345.0 18 14185.0 19 8698.0 20 8641.0 21 8921.0 22 9690.0 23 10509.0 24 11982.0 25 13592.0 26 15599.0 27 18176.0 28 21158.0 29 24842.0 30 29792.0 31 36500.0 32 44989.0 33 56853.0 34 74440.0 35 98945.0 36 128063.0 37 154968.0 38 102847.0 39 9623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.46068052813552 19.130429423402067 15.04902736205469 20.35986268640772 2 14.63643794205351 21.101200989049808 41.552105838186854 22.710255230709834 3 18.851417886616304 27.730036277607383 30.64210235563145 22.776443480144863 4 11.505805064427129 17.065256789517512 38.86184388191286 32.5670942641425 5 12.085108043389864 39.029108667704456 33.94577088476056 14.94001240414512 6 28.56933149651079 37.291535355069975 18.911846889480632 15.2272862589386 7 26.553152087773114 34.996940916575305 21.221416047664217 17.228490947987368 8 26.283431215847624 33.80165009285237 20.552761600900087 19.36215709039992 9 27.51056420449179 16.40716435807828 21.072354020968252 35.009917416461676 10-11 23.980395669880494 27.824513831812837 27.285019998080017 20.91007050022665 12-13 26.000301838442514 25.59071528524246 28.295403329052153 20.11357954726287 14-15 22.947598589488923 25.79798073122783 28.57403899950211 22.680381679781135 16-17 20.08758323846518 29.55759788699791 28.80587255263258 21.54894632190433 18-19 19.894442365932104 27.116733871898973 33.14606193761486 19.842761824554056 20-21 21.45406469056371 25.856209203998738 33.00756410085331 19.682162004584242 22-23 20.746257396290268 26.149084166177815 32.802191288120824 20.302467149411093 24-25 20.28253371928312 26.276251770647285 33.34559955656833 20.09561495350126 26-27 20.293666312542662 26.280312244992228 33.268667210683255 20.15735423178186 28-29 19.964265934944702 26.363856137795317 33.6579271675903 20.013950759669676 30-31 19.968892288264794 26.575636399102702 33.49135298012905 19.964118332503453 32-33 19.65130257229412 26.671549751517027 33.19483097922105 20.4823166969678 34-35 20.104709354548955 26.424483510843068 33.16920313101501 20.301604003592967 36-37 20.41631231481534 26.372194931925954 32.930009500136784 20.28148325312193 38-39 20.382559821160058 26.409375548482515 33.066554857489486 20.141509772867934 40-41 20.15469623466778 26.661288427085594 33.126722717365816 20.05729262088081 42-43 19.956056386605255 26.924881159265574 32.592899015913524 20.52616343821565 44-45 20.125338146635674 27.155092997702322 32.12509796052223 20.59447089513977 46-47 20.314925954573425 27.272419316840928 31.34836945057077 21.064285278014875 48-49 20.521194875000898 27.584442274980574 30.962133274203467 20.932229575815064 50-51 20.89265767578622 27.797723670053305 30.647550779036248 20.662067875124226 52-53 20.60667479865124 28.133124283830902 30.48236427183828 20.777836645679574 54-55 20.31797295577082 28.05217696600377 30.667698037589265 20.962152040636152 56-57 20.590280539781837 28.45817306582023 30.084805998260254 20.86674039613767 58-59 20.64835084759157 27.770234279503548 30.44591824900523 21.13549662389965 60-61 20.59232407040109 27.762778889936328 30.738408847502107 20.90648819216047 62-63 20.8429557645291 27.804771904758972 30.753598019472722 20.598674311239204 64-65 20.64178894614708 27.768261354620925 30.94164351421836 20.648306185013634 66-67 20.47716258074846 28.032108481590274 30.602357331559794 20.888371606101476 68-69 20.35778764704252 28.31478826184731 30.472814646107487 20.854609445002684 70-71 20.35630578824983 28.22127827293204 30.248836186479082 21.173579752339045 72-73 20.38921448879674 28.13917086639227 29.98424461391606 21.487370030894926 74-75 20.27797393724904 28.319069574571166 30.1418814612877 21.261075026892094 76-77 20.47809141178415 28.003904714295828 30.267694648057343 21.250309225862676 78-79 20.36353184817169 28.073507032747635 30.17950764350988 21.383453475570793 80-81 20.19530065184043 28.590344385863258 30.006061125501223 21.208293836795086 82-83 20.23794005618514 28.46838533931246 29.916635789132272 21.377038815370135 84-85 20.263335875112766 27.925620623512522 29.901281118410317 21.9097623829644 86-87 20.40191221582833 27.93860013731992 29.99660292271803 21.66288472413372 88-89 20.61970900013959 27.511659700953306 30.083824628244393 21.784806670662714 90-91 20.213823445038198 27.587360676828748 30.413005736889293 21.785810141243765 92-93 20.178143270051898 27.742150036129544 30.159852690008538 21.919854003810023 94-95 19.999414968192745 27.702231126565984 30.119387543543812 22.178966361697455 96-97 20.00688729975766 27.577579632478905 29.89354964860401 22.521983419159422 98-99 20.116461265030466 27.594729989186327 29.811250839766195 22.47755790601701 100-101 20.492029158423843 26.92792244947182 30.58268096363408 21.99736742847026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1912.0 1 1198.5 2 709.0 3 887.5 4 698.5 5 628.0 6 1048.5 7 2184.5 8 3424.5 9 3887.5 10 3441.5 11 2794.5 12 2740.0 13 2930.5 14 3065.5 15 3126.0 16 3160.5 17 3292.5 18 3447.0 19 3642.5 20 3862.0 21 4167.5 22 4709.0 23 5394.0 24 6092.5 25 6918.5 26 8221.0 27 10028.5 28 11469.0 29 13029.0 30 15107.0 31 16497.5 32 18032.5 33 20061.0 34 22585.0 35 24473.0 36 26704.0 37 29362.5 38 30342.0 39 30402.0 40 30589.5 41 31587.5 42 32064.5 43 32727.0 44 34383.5 45 35381.5 46 35116.0 47 35942.0 48 40897.5 49 40306.0 50 34152.0 51 30339.0 52 26947.5 53 24914.5 54 23645.5 55 21647.0 56 18833.0 57 16238.5 58 14468.0 59 13156.0 60 11492.0 61 9450.5 62 7420.0 63 5443.5 64 3861.0 65 2846.5 66 2045.0 67 1488.5 68 1068.0 69 749.5 70 562.0 71 418.5 72 334.0 73 275.5 74 219.5 75 160.5 76 101.0 77 63.0 78 45.0 79 31.5 80 19.0 81 14.5 82 13.5 83 7.5 84 3.5 85 3.0 86 4.0 87 3.5 88 2.5 89 3.0 90 1.5 91 1.5 92 1.5 93 0.0 94 1.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.274018067778241 2 0.2406237163405937 3 0.23908716642781852 4 0.23785792649759838 5 0.24021396969718697 6 0.21173657798042025 7 0.21153170465871687 8 0.21368287453660215 9 0.22218511738729152 10-11 0.22991908528159324 12-13 0.22351679397836335 14-15 0.21491211446682232 16-17 0.21040490138934842 18-19 0.20062220027801309 20-21 0.20548794166846784 22-23 0.20400261008611853 24-25 0.204156265077396 26-27 0.20261971516462082 28-29 0.2122999796151045 30-31 0.22310704733495662 32-33 0.215321861110229 34-35 0.2138877478583055 36-37 0.20656352660741048 38-39 0.19811250208714698 40-41 0.19631986052224257 42-43 0.20589768831187458 44-45 0.2016465668865299 46-47 0.20973906309381252 48-49 0.21368287453660215 50-51 0.22423385060432507 52-53 0.23365802340267952 54-55 0.21562917109278407 56-57 0.19775397377416606 58-59 0.19094193582752944 60-61 0.19273457739243383 62-63 0.1973954454611852 64-65 0.19196630243604623 66-67 0.19370772567052477 68-69 0.19667838883522348 70-71 0.19847103040012784 72-73 0.19903443203481205 74-75 0.19964905199992214 76-77 0.20548794166846784 78-79 0.2069732732508172 80-81 0.20195387686908492 82-83 0.19811250208714698 84-85 0.18745908935857236 86-87 0.1890980759321992 88-89 0.1963710788526684 90-91 0.19509062059202242 92-93 0.1988295587131087 94-95 0.19534671224415165 96-97 0.2003661086258839 98-99 0.2031831167993051 100-101 0.19263214073158214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.19827995645076 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19967456110018 46.674831125231506 2 11.567928539841763 13.464670873558552 3 3.4596457933632396 6.0403630329693305 4 1.483680143808766 3.453905295008388 5 0.8129924918646746 2.3657382322016427 6 0.5014014355046034 1.7508420668437916 7 0.3471086310812509 1.4140787700876916 8 0.23899969413263997 1.1127496886909987 9 0.174835602329214 0.915761819763926 >10 1.1000305891589681 11.883777615572889 >50 0.07869433353581134 3.124608693625377 >100 0.030587959320399493 3.1429715236615925 >500 0.0024753259767111434 0.9889699378189956 >1k 0.001414471986692082 1.3467525279549488 >5k 5.304269950095308E-4 2.319978797010412 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9775 1.0013183598251612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7466 0.7647921099186346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5305 0.5434264858181566 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2267 0.23222391015075605 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1983 0.20313189846887922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.1799812131163998 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1616 0.16553764393631307 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1523 0.15601103447710696 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1458 0.14935265152174781 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.14730391830471423 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1046 0.10714874725085613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0243666085167889E-4 0.0 10-11 0.0 0.0 0.0 1.0243666085167889E-4 0.0 12-13 0.0 0.0 0.0 1.5365499127751832E-4 0.0 14-15 0.0 0.0 0.0 3.0730998255503665E-4 0.0 16-17 0.0 0.0 0.0 3.0730998255503665E-4 0.0 18-19 0.0 0.0 0.0 3.0730998255503665E-4 0.0 20-21 0.0 0.0 0.0 4.0974664340671555E-4 0.0 22-23 0.0 0.0 0.0 6.146199651100733E-4 0.0 24-25 0.0 0.0 0.0 9.2192994766511E-4 0.0 26-27 0.0 0.0 0.0 0.0013316765910718256 0.0 28-29 0.0 0.0 0.0 0.002817008173421169 0.0 30-31 0.0 0.0 0.0 0.006658382955359128 0.0 32-33 0.0 0.0 0.0 0.010550976067722925 0.0 34-35 0.0 0.0 0.0 0.014187477527957525 0.0 36-37 0.0 0.0 0.0 0.019411747231393148 0.0 38-39 0.0 0.0 0.0 0.027248151786546583 0.0 40-41 0.0 0.0 0.0 0.038516184480231266 0.0 42-43 0.0 0.0 0.0 0.049528125521786745 0.0 44-45 0.0 0.0 0.0 0.05383046527755725 0.0 46-47 0.0 0.0 0.0 0.06125712318930397 0.0 48-49 0.0 0.0 0.0 0.07078373264851011 0.0 50-51 0.0 0.0 0.0 0.08112983539452968 0.0 52-53 0.0 0.0 0.0 0.10776336721596619 0.0 54-55 0.0 0.0 0.0 0.12430688794351233 0.0 56-57 0.0 0.0 0.0 0.17132531527443293 0.0 58-59 0.0 0.0 0.0 0.2553233771728096 0.0 60-61 0.0 0.0 0.0 0.32994848460325765 0.0 62-63 0.0 0.0 0.0 0.3701036556571158 0.0 64-65 0.0 0.0 0.0 0.4496969411388703 0.0 66-67 0.0 0.0 0.0 0.5884986165928952 0.0 68-69 0.0 0.0 0.0 0.7867135553408938 0.0 70-71 0.0 0.0 0.0 0.9873869739493327 0.0 72-73 0.0 0.0 0.0 1.2218132723083999 0.0 74-75 0.0 0.0 0.0 1.4931679869044974 0.0 76-77 0.0 0.0 0.0 1.67729788478539 0.0 78-79 0.0 0.0 0.0 1.9204825176472757 0.0 80-81 0.0 0.0 0.0 2.267742797934467 0.0 82-83 0.0 0.0 0.0 2.5786380636193127 0.0 84-85 0.0 0.0 0.0 2.9566293421620076 0.0 86-87 0.0 0.0 0.0 3.431320828548688 0.0 88-89 0.0 0.0 0.0 4.042816475502785 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11305 0.0 29.423216 1 TACCTGG 1010 0.0 29.162277 2 GGTATCA 7825 0.0 27.934193 1 GTACCTG 1150 0.0 26.445076 1 CCATGCG 130 1.0008729E-5 25.58031 9 GTACATG 8120 0.0 24.695572 1 TACATGG 8085 0.0 24.3848 2 TATCAAC 13605 0.0 24.303108 2 ATCAACG 13780 0.0 23.959995 3 TCAACGC 13835 0.0 23.868423 4 GAGTACT 7305 0.0 23.862663 12-13 ATGGGAG 1835 0.0 23.822763 5 CAACGCA 13965 0.0 23.613419 5 ACCTGGG 1195 0.0 23.45499 3 ACATGGG 8105 0.0 23.269585 3 AACGCAG 14200 0.0 23.250124 6 AGTACTT 7500 0.0 22.450605 12-13 GTACTTT 7760 0.0 22.431168 14-15 ACGGTTC 55 0.0028805826 21.58767 92-93 ACGCAGA 15405 0.0 21.340054 7 >>END_MODULE