FastQCFastQC Report
Thu 26 May 2016
SRR937391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976213
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104831.0738435157081498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85480.8756285769601512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.6138004718232599No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27130.27791066089060484No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24890.25496484885982873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20900.21409262118000888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.2091756614591283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.1790592831687347No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.12425566961308648No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12130.12425566961308648No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1053048873555259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10000.033.2492452
TCTAGCG300.003951565431.66676128-29
GTACCTG11900.029.145461
GTATCAA130700.026.1002031
ACCTGGG13250.024.7352893
GGTATCA94950.024.6187021
GTACATG88450.023.6884021
GAGTACT76300.023.03473512-13
TACATGG89150.023.0168652
CATGGGG22300.022.7909624
GCCCCGT1050.002195621822.6196948
ATGGGGA12000.022.5619875
AGTACTT77600.022.4958112-13
ACATGGG90150.022.0239053
TATCAAC154500.021.9202142
ATCAACG155450.021.7251423
TCAACGC156750.021.5752684
GTACTTT81450.021.54800814-15
GCCTACA2657.894414E-1021.5144561
CAACGCA158600.021.32365
Produced by FastQC (version 0.11.5)