FastQCFastQC Report
Thu 26 May 2016
SRR937390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981115
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102091.0405508018937637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74460.7589324391126423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53750.5478460730903105No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22930.23371368290159666No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20680.21078059146990924No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.17296647182032687No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16870.17194722331225187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14490.14768910882006694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.14728140941683698No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12400.12638681500129953No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.1126269601422871No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9860.10049790289619463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT753.0747024E-431.672667
GACGCGT600.003947010331.672668
GTATCAA111000.031.4795591
TACCTGG10100.029.6418912
TATCAAC131500.026.52512
GGTATCA73000.026.3101181
ATCAACG132300.026.2915233
TCAACGC133150.026.1290264
GTACATG80300.025.8720111
CAACGCA134750.025.7482325
TACATGG79750.025.563092
AACGCAG137350.025.3183886
CATGGGT7950.024.5115074
GAGTACT69850.024.48316412-13
ACATGGG78900.024.2110483
GTACCTG12600.024.1475871
TATCACG1000.001649196523.7605652
CGGGTCG1000.001652141723.7532836
CGCAGAG150000.023.0260248
ACGCAGA150900.022.951667
Produced by FastQC (version 0.11.5)