##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937390_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 981115 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7372040994175 33.0 31.0 34.0 30.0 34.0 2 31.811390102077738 33.0 31.0 34.0 30.0 34.0 3 31.825873623377483 33.0 31.0 34.0 30.0 34.0 4 35.47650071602208 37.0 35.0 37.0 33.0 37.0 5 35.49912701365283 37.0 35.0 37.0 33.0 37.0 6 35.56070287377117 37.0 35.0 37.0 33.0 37.0 7 35.51070873445009 37.0 35.0 37.0 33.0 37.0 8 35.514185391111134 37.0 35.0 37.0 33.0 37.0 9 37.10733094489433 39.0 37.0 39.0 33.0 39.0 10-11 36.98789744321512 39.0 37.0 39.0 33.0 39.0 12-13 36.85274305254735 39.0 37.0 39.0 33.0 39.0 14-15 37.952583030531585 40.0 38.0 41.0 33.0 41.0 16-17 37.93374324110833 40.0 38.0 41.0 33.0 41.0 18-19 37.99299011838571 40.0 38.0 41.0 33.0 41.0 20-21 37.98642259062393 40.0 38.0 41.0 33.0 41.0 22-23 37.83264092384685 40.0 37.5 41.0 33.0 41.0 24-25 37.67640898365635 40.0 37.0 41.0 32.0 41.0 26-27 37.494942998527186 40.0 37.0 41.0 32.0 41.0 28-29 37.5306778512203 40.0 37.0 41.0 32.0 41.0 30-31 37.31363550654103 39.0 36.5 41.0 32.0 41.0 32-33 37.05292702690306 39.0 36.0 41.0 31.5 41.0 34-35 36.89192500369477 39.0 35.0 41.0 31.0 41.0 36-37 36.83697273000617 39.0 35.0 41.0 31.0 41.0 38-39 36.7134938309984 39.0 35.0 41.0 31.0 41.0 40-41 36.48825672831421 39.0 35.0 41.0 30.5 41.0 42-43 36.18839789423258 39.0 35.0 41.0 30.0 41.0 44-45 35.84810598145987 39.0 35.0 41.0 29.0 41.0 46-47 35.32059136798439 39.0 35.0 41.0 26.0 41.0 48-49 34.869111164338534 39.0 35.0 40.5 24.0 41.0 50-51 33.95136349969168 38.0 34.0 39.5 22.5 40.5 52-53 34.01610259755482 38.0 34.0 39.5 22.0 40.5 54-55 34.33540359692799 38.0 34.0 40.0 22.0 41.0 56-57 34.153034557620664 38.0 34.0 40.0 20.0 41.0 58-59 33.926679339323115 38.0 34.0 40.0 18.5 41.0 60-61 33.64728344791385 38.0 34.0 40.0 17.5 41.0 62-63 33.3226380189886 37.5 33.0 40.0 14.0 41.0 64-65 32.96011731550328 37.0 33.0 40.0 10.0 41.0 66-67 32.59071464609144 36.5 32.5 39.5 8.0 41.0 68-69 32.136798948135535 36.0 32.0 39.0 6.5 41.0 70-71 31.690070481034333 35.5 31.0 39.0 2.0 40.0 72-73 31.288873883285852 35.0 31.0 38.0 2.0 40.0 74-75 30.839729287596256 35.0 31.0 37.0 2.0 39.5 76-77 30.39645046707063 35.0 30.5 37.0 2.0 39.0 78-79 29.927385678539213 34.5 30.0 36.0 2.0 39.0 80-81 29.441913027524805 34.0 29.0 36.0 2.0 37.5 82-83 28.966816835946858 34.0 29.0 35.0 2.0 37.0 84-85 28.535741987432665 34.0 29.0 35.0 2.0 37.0 86-87 28.093070129393595 34.0 27.5 35.0 2.0 36.0 88-89 27.67951361461195 34.0 27.0 35.0 2.0 36.0 90-91 27.256548416852254 33.5 26.0 35.0 2.0 35.5 92-93 26.822834733950657 33.0 25.5 35.0 2.0 35.0 94-95 26.321126473451123 33.0 25.0 35.0 2.0 35.0 96-97 25.715139917338945 33.0 23.0 35.0 2.0 35.0 98-99 24.976177104620763 32.0 18.0 34.5 2.0 35.0 100-101 23.132069635058073 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13446759128615327 1101 2 0.0423437680261074 1101 3 -0.0177512760210341 1101 4 0.031856626039136415 1101 5 0.01283452275671948 1101 6 0.18413889989550825 1101 7 0.11844028426205 1101 8 0.12271337646906488 1101 9 0.07852623601574749 1101 10-11 0.036570221697367344 1101 12-13 0.15712878803571328 1101 14-15 0.16847546459559481 1101 16-17 0.3235484466093723 1101 18-19 0.17709590907792716 1101 20-21 0.21791499015812832 1101 22-23 0.2868302915820564 1101 24-25 0.17925334245469315 1101 26-27 0.19975611380233715 1101 28-29 0.145495309761138 1101 30-31 0.17441025417010536 1101 32-33 0.2903747446483109 1101 34-35 0.223383025211497 1101 36-37 0.30711912958543763 1101 38-39 0.2907179321629698 1101 40-41 0.39359315197556555 1101 42-43 0.46409777333547453 1101 44-45 0.39859397825474474 1101 46-47 0.5750465702072418 1101 48-49 0.890594686290676 1101 50-51 0.7036304164637386 1101 52-53 0.5515984710687718 1101 54-55 0.8081024323273098 1101 56-57 0.8431839199071831 1101 58-59 1.0914032984518443 1101 60-61 0.9267666352338182 1101 62-63 0.8339094249357117 1101 64-65 0.8444127000057335 1101 66-67 0.870807386397253 1101 68-69 1.0368950305539961 1101 70-71 1.0153790602877102 1101 72-73 0.741253811288292 1101 74-75 0.8876268580237934 1101 76-77 0.9309437647720671 1101 78-79 0.8808144900106036 1101 80-81 0.9760866878182277 1101 82-83 0.7337275596200534 1101 84-85 1.0030184500082697 1101 86-87 0.9573552150314484 1101 88-89 1.014900254084509 1101 90-91 1.096477058839465 1101 92-93 0.9993299363803771 1101 94-95 1.0260783628943635 1101 96-97 0.9861616399002315 1101 98-99 0.8346808681791593 1101 100-101 1.3366340470390945 1102 1 0.03332217831388817 1102 2 0.01043778077043811 1102 3 0.20498065577026736 1102 4 0.010453602664966866 1102 5 0.023757519179987696 1102 6 0.006645046276133826 1102 7 0.09952376752167424 1102 8 0.08244114377389877 1102 9 0.16879595998412356 1102 10-11 0.14647252470855676 1102 12-13 0.022379338163652562 1102 14-15 0.2072671900978733 1102 16-17 0.19264200778287943 1102 18-19 0.3127180374817371 1102 20-21 0.24830929033088012 1102 22-23 0.4036086563222554 1102 24-25 0.13917392333245715 1102 26-27 0.07367288141539063 1102 28-29 0.04488425559414111 1102 30-31 0.1925132266529559 1102 32-33 0.14460051572340404 1102 34-35 0.24023593497618378 1102 36-37 0.3852756454656756 1102 38-39 0.26646893744563727 1102 40-41 0.36457793475566547 1102 42-43 0.3900455344760161 1102 44-45 0.19269022734980723 1102 46-47 0.5285387178924097 1102 48-49 0.678699737432872 1102 50-51 0.621621213020326 1102 52-53 0.5040437629053756 1102 54-55 0.627022891352631 1102 56-57 0.7800346076567166 1102 58-59 0.7373603060425324 1102 60-61 0.9020617690206336 1102 62-63 0.657900020794159 1102 64-65 0.6059442418692171 1102 66-67 0.6113376595617623 1102 68-69 0.6094542185866523 1102 70-71 0.6062601968126167 1102 72-73 0.6936388936820457 1102 74-75 0.8353435582596092 1102 76-77 0.7715632769322234 1102 78-79 0.6099651784769087 1102 80-81 0.49821831712875664 1102 82-83 0.8762698316663844 1102 84-85 0.9487195674668563 1102 86-87 0.8650129243488607 1102 88-89 0.9136107685168575 1102 90-91 0.872344182333336 1102 92-93 0.9556601708388186 1102 94-95 0.9848990462715399 1102 96-97 1.1222819297656912 1102 98-99 1.0040342617697213 1102 100-101 1.6360491553085765 1103 1 0.06520769137086546 1103 2 0.12479548747571201 1103 3 -0.03010170063361528 1103 4 -0.043520115299550355 1103 5 -0.016811479193727052 1103 6 -0.03773380886937616 1103 7 -0.020878911928186028 1103 8 -0.0025455553437794265 1103 9 0.08254528485509383 1103 10-11 0.19872097518004495 1103 12-13 0.06740063974519472 1103 14-15 0.15019292104877735 1103 16-17 0.010836763178865283 1103 18-19 0.029238547584206742 1103 20-21 0.12512877707593617 1103 22-23 0.13270767001757378 1103 24-25 -0.0607449491292229 1103 26-27 0.1011217789100769 1103 28-29 -0.037524813956657965 1103 30-31 0.006780467935662671 1103 32-33 -0.016526544493991935 1103 34-35 0.0788182798010908 1103 36-37 0.12171647726946588 1103 38-39 0.09207647887036075 1103 40-41 0.03559456141883288 1103 42-43 0.01672588674583153 1103 44-45 -0.05924551605409789 1103 46-47 0.08605410859321694 1103 48-49 0.3004209261380666 1103 50-51 0.2970348626165986 1103 52-53 0.28254429292368854 1103 54-55 0.3283745687728086 1103 56-57 0.2008119977255447 1103 58-59 0.3759528104855008 1103 60-61 0.19432559338474675 1103 62-63 0.12206084754801338 1103 64-65 0.10488457876513735 1103 66-67 0.12314186579304476 1103 68-69 0.13784213372097653 1103 70-71 0.016323672014557644 1103 72-73 0.22506087416512344 1103 74-75 0.029536440102507555 1103 76-77 0.18208057330845406 1103 78-79 0.28008200661427196 1103 80-81 0.3485253094111833 1103 82-83 0.47927677979929584 1103 84-85 0.4294547652395089 1103 86-87 0.4057955735140908 1103 88-89 0.5087115168175487 1103 90-91 0.5783916949824324 1103 92-93 0.3274241270538525 1103 94-95 0.4495637452592369 1103 96-97 0.35377256237373444 1103 98-99 0.607977612934679 1103 100-101 0.8664715339590323 1104 1 0.0802743235259804 1104 2 0.06436736875012272 1104 3 0.06679459946480293 1104 4 0.005828479158999755 1104 5 -0.04399663805654086 1104 6 0.0014353401430255985 1104 7 0.03758745905052763 1104 8 -0.1616949918257049 1104 9 0.056165882987080806 1104 10-11 0.04747117442575899 1104 12-13 0.05503460964117579 1104 14-15 0.17765336803186216 1104 16-17 0.13169153827615077 1104 18-19 0.1882494260348082 1104 20-21 0.06305733816357417 1104 22-23 0.07743069287854354 1104 24-25 0.02620222626111257 1104 26-27 0.06907868041614762 1104 28-29 -0.06042381356036941 1104 30-31 0.15145918147370452 1104 32-33 0.19439753155425876 1104 34-35 0.2599571317655389 1104 36-37 0.38688803331637445 1104 38-39 0.34645184171692023 1104 40-41 0.5746010235606889 1104 42-43 0.6861403163766298 1104 44-45 0.7343991670464973 1104 46-47 0.6679046150794861 1104 48-49 0.7174261277308531 1104 50-51 0.650152747350937 1104 52-53 0.6073415009976344 1104 54-55 0.7284906796718786 1104 56-57 0.8473694704270471 1104 58-59 0.8534625387947656 1104 60-61 0.8300071745987623 1104 62-63 0.6981449270474229 1104 64-65 0.9841003622431685 1104 66-67 1.1539010175692894 1104 68-69 1.0630789253750272 1104 70-71 1.0236617638474534 1104 72-73 1.1755492272475436 1104 74-75 0.9626194915671249 1104 76-77 1.0057806503745041 1104 78-79 0.9594601844920767 1104 80-81 0.854665733406069 1104 82-83 0.9311591217298023 1104 84-85 1.1627835652757739 1104 86-87 1.2456067690030252 1104 88-89 0.9207520269450775 1104 90-91 0.8344122059581274 1104 92-93 1.274733688362307 1104 94-95 1.4228651846541744 1104 96-97 1.4480475931197248 1104 98-99 1.6002732014516887 1104 100-101 1.6828455836117975 1105 1 0.12996257985657778 1105 2 0.04181273233066207 1105 3 0.056206874146013774 1105 4 0.10145395327668894 1105 5 0.00990503445005686 1105 6 0.09478071456053527 1105 7 -0.09210657700047875 1105 8 0.06640600878839109 1105 9 0.03783694464947018 1105 10-11 0.04152889938331583 1105 12-13 0.09854253869511354 1105 14-15 0.07526814966680462 1105 16-17 0.13810269313653833 1105 18-19 0.09328898099634131 1105 20-21 0.045194751016232715 1105 22-23 0.11750727417392426 1105 24-25 0.03211777009668282 1105 26-27 0.0031515771397678805 1105 28-29 0.06447016469343936 1105 30-31 0.09421683057242802 1105 32-33 0.03784441231140079 1105 34-35 0.18864766386105458 1105 36-37 -0.009812468796845053 1105 38-39 0.34419955692119686 1105 40-41 0.16427013456743822 1105 42-43 0.4118188916755514 1105 44-45 0.40524950841123797 1105 46-47 0.38694915504986227 1105 48-49 0.4256149078486189 1105 50-51 0.31538881280626896 1105 52-53 0.3276033945785315 1105 54-55 0.42605741738093883 1105 56-57 0.483491639267136 1105 58-59 0.47405496013638526 1105 60-61 0.6425939607129791 1105 62-63 0.41806979068214645 1105 64-65 0.4029585062304619 1105 66-67 0.40756138674893094 1105 68-69 0.491484445008016 1105 70-71 0.5427326737921874 1105 72-73 0.4761474283095666 1105 74-75 0.6645004293267647 1105 76-77 0.5806518709792066 1105 78-79 0.45391454276249377 1105 80-81 0.3727439763710656 1105 82-83 0.7792489241356115 1105 84-85 0.894989612308251 1105 86-87 0.6951906291337906 1105 88-89 0.5730855890590156 1105 90-91 0.6760532853209149 1105 92-93 0.6806170825031153 1105 94-95 0.8737783804783241 1105 96-97 0.9570408380714994 1105 98-99 1.1481748017117788 1105 100-101 1.414288346318152 1106 1 -0.05948846882972347 1106 2 -0.05042850337767746 1106 3 -0.08794774083089152 1106 4 0.04362098591089847 1106 5 0.02096810706306229 1106 6 -0.081078299598623 1106 7 0.04556889812634779 1106 8 -0.032392355715280985 1106 9 0.16590160732835812 1106 10-11 -0.40642483727149425 1106 12-13 -0.39052348689737215 1106 14-15 -0.3863567462217361 1106 16-17 -0.28681742498264384 1106 18-19 -0.1025988057833942 1106 20-21 -0.04485522248267415 1106 22-23 0.0935514174312928 1106 24-25 -0.03330511170842243 1106 26-27 -0.0010798910210496615 1106 28-29 0.07881785909791716 1106 30-31 -0.03953345336790903 1106 32-33 0.1778508482923229 1106 34-35 0.04448093409653353 1106 36-37 0.19452538234270378 1106 38-39 0.23245199880807377 1106 40-41 0.1773605411699961 1106 42-43 0.16443379286431536 1106 44-45 0.3424816580288663 1106 46-47 0.3443210033121531 1106 48-49 0.3092345792350031 1106 50-51 0.522699708543378 1106 52-53 0.43655773697271627 1106 54-55 0.310818528912705 1106 56-57 0.3024262951314469 1106 58-59 0.36460996884389374 1106 60-61 0.4498563972996479 1106 62-63 0.25411544828796195 1106 64-65 0.5791624132494633 1106 66-67 0.5079195301577286 1106 68-69 0.5028269149103473 1106 70-71 0.34559214225588164 1106 72-73 0.4341363735658703 1106 74-75 0.24578838268482883 1106 76-77 0.53783253274948 1106 78-79 0.6442552711640985 1106 80-81 0.7215294689129017 1106 82-83 0.7070481972483584 1106 84-85 0.6300058915541058 1106 86-87 0.7417799378337193 1106 88-89 0.7893951218825173 1106 90-91 0.6504342953913316 1106 92-93 0.8222726439478052 1106 94-95 1.0419168676442219 1106 96-97 0.80410375924966 1106 98-99 0.8690622099044027 1106 100-101 0.8301004156753109 1107 1 0.11005670308682625 1107 2 0.05316130466317759 1107 3 0.1337280320990324 1107 4 0.09526871243195956 1107 5 0.10690653725793453 1107 6 0.04153544049583502 1107 7 0.016884168866795335 1107 8 0.1325754306028344 1107 9 0.04293067240396198 1107 10-11 0.07173336034986022 1107 12-13 0.16994331382759498 1107 14-15 0.232217054198955 1107 16-17 0.12855551287868394 1107 18-19 0.005006136734699851 1107 20-21 -0.032305446721643705 1107 22-23 0.09328517498860833 1107 24-25 0.12734160937533545 1107 26-27 0.10784468795208113 1107 28-29 0.2555747284836798 1107 30-31 0.3560763575834187 1107 32-33 0.28334073438686147 1107 34-35 0.23503776114808517 1107 36-37 0.010786676975520493 1107 38-39 0.14851617481252077 1107 40-41 -0.09103003153237665 1107 42-43 0.2101729215735233 1107 44-45 0.1014727149286756 1107 46-47 0.32341518368230737 1107 48-49 0.21941661630389575 1107 50-51 0.5062687463663451 1107 52-53 0.45953727103190545 1107 54-55 0.251066117847607 1107 56-57 0.16157081351375524 1107 58-59 0.28670346128326685 1107 60-61 0.3312544843228906 1107 62-63 0.4592957655298875 1107 64-65 0.5035082175331524 1107 66-67 0.38971119296581236 1107 68-69 0.34216861806821086 1107 70-71 0.34325549274338485 1107 72-73 0.2693207257864323 1107 74-75 0.3884033795759123 1107 76-77 0.519818999034257 1107 78-79 0.4747497875619864 1107 80-81 0.42132540482950276 1107 82-83 0.5154412835439821 1107 84-85 0.5681103115093862 1107 86-87 0.5531116130774727 1107 88-89 0.5231923675134915 1107 90-91 0.7011046971693702 1107 92-93 0.7083225321590518 1107 94-95 0.9516590258947595 1107 96-97 0.7968209535504194 1107 98-99 0.8220844750488006 1107 100-101 1.1471935112315457 1108 1 0.12491620309801377 1108 2 0.09144519423220032 1108 3 -0.008186386130965673 1108 4 0.0022168372341724307 1108 5 0.005592636213648916 1108 6 -0.011974741797743604 1108 7 0.04708663205168051 1108 8 0.14363035521454037 1108 9 0.006567849682312499 1108 10-11 0.22768539121478426 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-1.529359159647445 2316 80-81 -1.4291303399903903 2316 82-83 -1.078972027842088 2316 84-85 -1.312777227252809 2316 86-87 -1.8671099248339615 2316 88-89 -2.0856808623717775 2316 90-91 -2.31331900217776 2316 92-93 -2.128268983620167 2316 94-95 -2.6848031798053604 2316 96-97 -2.614107674683872 2316 98-99 -2.7009878954407576 2316 100-101 -3.124773789809719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3360.0 3 678.0 4 606.0 5 651.0 6 752.0 7 933.0 8 1183.0 9 1455.0 10 1965.0 11 2694.0 12 4003.0 13 5859.0 14 6530.0 15 8514.0 16 11442.0 17 15728.0 18 12863.0 19 7190.0 20 6721.0 21 6913.0 22 7270.0 23 7893.0 24 8858.0 25 9737.0 26 11367.0 27 13172.0 28 15354.0 29 18465.0 30 22582.0 31 28089.0 32 35272.0 33 45835.0 34 62851.0 35 89911.0 36 129113.0 37 186968.0 38 164938.0 39 23396.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44118233328461 19.198093775842842 14.979826612107713 20.380897278764838 2 14.61775993918165 21.075213661240156 41.57504404016954 22.731982359408658 3 18.95126516832808 27.7936494312771 30.497995267113797 22.757090133281018 4 11.553517800778021 16.999868184015117 38.78864175378614 32.65797226142073 5 12.134028456165161 39.08921469649346 33.80457129777607 14.972185549565308 6 28.550376830538593 37.250668899736525 18.95917158554768 15.239782684177202 7 26.569362146199804 35.10682292299539 21.161078327960617 17.162736602844188 8 26.510605794484903 33.65476127261364 20.373725078527404 19.46090785437405 9 27.50357894822829 16.450296016288554 21.09118608692811 34.95493894855504 10-11 23.910835447873044 27.823785009909273 27.31221023486583 20.953169307351857 12-13 26.03646587017995 25.59136534598562 28.28826501173897 20.08390377209546 14-15 22.97331519763419 25.774596541963312 28.55959047630718 22.69249778409532 16-17 20.073208303612777 29.588909181979666 28.797750955950946 21.54013155845661 18-19 19.919770124097454 27.091853354041774 33.156627465636554 19.831749056224226 20-21 21.3763249160544 25.948724389380967 32.961557842436015 19.71339285212862 22-23 20.763000456434035 26.166944577471764 32.78166421771597 20.28839074837823 24-25 20.301544889184754 26.23672305020405 33.39103737608776 20.07069468452344 26-27 20.364973154879777 26.325013566778928 33.20126463310478 20.108748645236517 28-29 19.963770927701148 26.326323055495422 33.70181880562511 20.008087211178324 30-31 20.042165869440005 26.524925757841896 33.54060545654332 19.892302916174785 32-33 19.642131404332492 26.746199828856998 33.12331894887971 20.488349817930803 34-35 20.135914923732315 26.511579933119933 33.041245772764015 20.31125937038374 36-37 20.456669959591473 26.332779407222485 32.943287666337376 20.26726296684867 38-39 20.392625546135772 26.47702987907668 32.98190387601964 20.148440698767896 40-41 20.164385939386513 26.629654304537713 33.10416299733766 20.101796758738107 42-43 19.960402267220367 26.990020922061493 32.53722625948462 20.512350551233524 44-45 20.169852464029905 27.250986781140597 32.08040677183823 20.498753982991268 46-47 20.384111295880373 27.336019266833077 31.25596408453185 21.0239053527547 48-49 20.560909905990812 27.602765744955914 30.91391920070747 20.922405148345803 50-51 20.93336145021856 27.799259736194852 30.597332333924438 20.670046479662147 52-53 20.57597344567854 28.182765224052087 30.514183582280097 20.727077747989277 54-55 20.317387215261874 28.130906781227377 30.608602060499734 20.943103943011018 56-57 20.590064077693693 28.501351798381926 30.073787363339715 20.834796760584663 58-59 20.607835950047427 27.823309771505063 30.460410308665736 21.108443969781774 60-61 20.588922967597547 27.806524597930228 30.762268335116683 20.842284099355545 62-63 20.713799976311158 27.881206578963493 30.76307685395828 20.64191659076707 64-65 20.69150527391589 27.74500404821924 30.90843469196268 20.65505598590219 66-67 20.417669772465995 28.028358541152354 30.62234419444223 20.93162749193942 68-69 20.326950495443736 28.42577481519446 30.46650988207464 20.780764807287166 70-71 20.328349415729384 28.277891142744387 30.231211777613158 21.162547663913063 72-73 20.32866023654266 28.209144267334967 30.039039187748017 21.42315630837436 74-75 20.324266869244116 28.377333176090758 30.09714834458792 21.201251610077207 76-77 20.504771157509204 28.09747445946367 30.249051818277888 21.148702564749236 78-79 20.354869604105364 28.093162970874065 30.279752338671965 21.27221508634861 80-81 20.207531372208663 28.631467167669012 30.02430133657351 21.136700123548813 82-83 20.23098517633257 28.56331876168748 29.832751947977027 21.37294411400292 84-85 20.240241068042096 28.082915890102296 29.824615138015332 21.852227903840273 86-87 20.34914408753207 28.11162237960716 30.026973468602247 21.51226006425852 88-89 20.566669185014874 27.642478953075006 30.10179912157292 21.6890527403372 90-91 20.175928048276838 27.78455322944005 30.36313301195741 21.676385710325704 92-93 20.148051798220337 27.974193798691005 30.14693382898338 21.73082057410528 94-95 19.833421475520634 27.931867790802855 30.142381962637245 22.09232877103927 96-97 19.875140059060897 27.676895551035095 29.95770790634994 22.490256483554067 98-99 20.00465577154864 27.846312572778338 29.79821416447023 22.350817491202786 100-101 19.9880450185195 27.78563319544098 30.368001078092266 21.858320707947254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1742.0 1 1135.0 2 760.0 3 963.5 4 748.0 5 667.5 6 1129.0 7 2296.5 8 3630.5 9 4111.0 10 3534.5 11 2908.5 12 2863.5 13 2975.5 14 3126.5 15 3234.0 16 3272.5 17 3390.5 18 3557.5 19 3759.0 20 3968.0 21 4219.5 22 4715.5 23 5377.5 24 6065.5 25 7010.5 26 8394.0 27 9990.5 28 11324.0 29 12975.5 30 15029.5 31 16442.0 32 18049.0 33 20197.0 34 22544.0 35 24523.5 36 26813.5 37 29301.5 38 30271.5 39 30415.0 40 30589.5 41 31411.0 42 32069.5 43 32706.5 44 34338.5 45 35447.5 46 35256.0 47 36138.0 48 42169.0 49 41073.5 50 33927.0 51 30501.0 52 26948.5 53 25064.5 54 23829.0 55 21763.5 56 18908.0 57 16180.0 58 14658.0 59 13437.5 60 11547.5 61 9555.0 62 7489.0 63 5470.5 64 3881.0 65 2794.5 66 1986.5 67 1461.0 68 1037.0 69 711.5 70 534.5 71 409.5 72 324.5 73 261.5 74 207.0 75 143.5 76 94.5 77 64.5 78 42.5 79 24.5 80 17.0 81 17.0 82 11.5 83 9.0 84 6.5 85 1.0 86 2.0 87 3.0 88 3.0 89 2.5 90 2.5 91 2.5 92 1.5 93 2.0 94 1.5 95 1.0 96 3.0 97 4.5 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2900781253981439 2 0.2510409075388716 3 0.2477793123130316 4 0.2525697803009841 5 0.2534871039582516 6 0.19314759228021178 7 0.18183393384057933 8 0.18743980063499183 9 0.18112045988492684 10-11 0.2019640918750605 12-13 0.1934533668326343 14-15 0.18662440182853182 16-17 0.18901963582250808 18-19 0.18387243085672933 20-21 0.1794386998466031 22-23 0.18183393384057933 24-25 0.17245684756628937 26-27 0.17332320879815313 28-29 0.18254740779623183 30-31 0.1923321934737518 32-33 0.1851974539172268 34-35 0.19360625410884555 36-37 0.1776040525320681 38-39 0.17673769130020436 40-41 0.17454630700784313 42-43 0.17999928652604436 44-45 0.1829041447740581 46-47 0.1850445666410156 48-49 0.1884590491430668 50-51 0.19054850858462055 52-53 0.20283045310692427 54-55 0.18733787578418432 56-57 0.17113182450579187 58-59 0.17388379547759436 60-61 0.17153952390902188 62-63 0.17847041376393186 64-65 0.17041835055013937 66-67 0.1688894777880269 68-69 0.16710579289889563 70-71 0.17648287917318561 72-73 0.17475015670945812 74-75 0.17699250342722309 76-77 0.1774002028304531 78-79 0.17459726943324685 80-81 0.17785886465908685 82-83 0.17342513364896062 84-85 0.16720771774970314 86-87 0.1665452062194544 88-89 0.16685098077187688 90-91 0.17128471178200314 92-93 0.16924621476585314 94-95 0.16685098077187688 96-97 0.16486344618113066 98-99 0.17179433603604063 100-101 0.1637932352476519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 981115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.952111297296824 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15851479028477 46.45355170552597 2 11.461398968149556 13.284245372498521 3 3.5044644767618323 6.092733461841744 4 1.5117612477053535 3.5043902432784386 5 0.8239379203361586 2.3874471030692175 6 0.5253689885582595 1.8267745258245913 7 0.33761688702062004 1.3695927988726104 8 0.25111361093153806 1.164205114317647 9 0.18910925213160848 0.9863352384191589 >10 1.1199577511333174 11.922088498121216 >50 0.08317584167813045 3.305040086662698 >100 0.02936075582414393 3.0813772938715127 >500 0.0022855676375689855 0.9528498865136635 >1k 0.0014065031615809142 1.3229045045437775 >5k 3.5162579039522854E-4 1.3062968771376904 >10k+ 1.7581289519761427E-4 1.040167289501496 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10209 1.0405508018937637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7446 0.7589324391126423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5375 0.5478460730903105 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2293 0.23371368290159666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2068 0.21078059146990924 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1697 0.17296647182032687 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1687 0.17194722331225187 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1449 0.14768910882006694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1445 0.14728140941683698 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1240 0.12638681500129953 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.1126269601422871 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 986 0.10049790289619463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0192485080749963E-4 0.0 18-19 0.0 0.0 0.0 1.0192485080749963E-4 0.0 20-21 0.0 0.0 0.0 1.0192485080749963E-4 0.0 22-23 0.0 0.0 0.0 3.567369778262487E-4 0.0 24-25 0.0 0.0 0.0 7.644363810562472E-4 0.0 26-27 0.0 0.0 0.0 0.0012740606350937454 0.0 28-29 0.0 0.0 0.0 0.0021404218669574923 0.0 30-31 0.0 0.0 0.0 0.004841430413356232 0.0 32-33 0.0 0.0 0.0 0.00784821351217747 0.0 34-35 0.0 0.0 0.0 0.011721357842862459 0.0 36-37 0.0 0.0 0.0 0.01630797612919994 0.0 38-39 0.0 0.0 0.0 0.023799452663551164 0.0 40-41 0.0 0.0 0.0 0.03236114013138113 0.0 42-43 0.0 0.0 0.0 0.039954541516539856 0.0 44-45 0.0 0.0 0.0 0.04321613674237984 0.0 46-47 0.0 0.0 0.0 0.04882200353679232 0.0 48-49 0.0 0.0 0.0 0.056670217048969795 0.0 50-51 0.0 0.0 0.0 0.06614922817406726 0.0 52-53 0.0 0.0 0.0 0.09005060568842592 0.0 54-55 0.0 0.0 0.0 0.10508452118253211 0.0 56-57 0.0 0.0 0.0 0.14554868695310946 0.0 58-59 0.0 0.0 0.0 0.22744530457693543 0.0 60-61 0.0 0.0 0.0 0.2976205643578989 0.0 62-63 0.0 0.0 0.0 0.3361991203885375 0.0 64-65 0.0 0.0 0.0 0.4022464237117973 0.0 66-67 0.0 0.0 0.0 0.538774761368443 0.0 68-69 0.0 0.0 0.0 0.7168884381545486 0.0 70-71 0.0 0.0 0.0 0.8898549099748756 0.0 72-73 0.0 0.0 0.0 1.0930930624850297 0.0 74-75 0.0 0.0 0.0 1.3400060135661978 0.0 76-77 0.0 0.0 0.0 1.4982953068702445 0.0 78-79 0.0 0.0 0.0 1.7319070649210337 0.0 80-81 0.0 0.0 0.0 2.05449921772677 0.0 82-83 0.0 1.0192485080749963E-4 0.0 2.3492658862620592 0.0 84-85 0.0 1.0192485080749963E-4 0.0 2.6949949802010975 0.0 86-87 0.0 1.0192485080749963E-4 0.0 3.150242326332795 0.0 88-89 0.0 1.0192485080749963E-4 0.0 3.764798214276614 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCT 75 3.0747024E-4 31.67266 7 GACGCGT 60 0.0039470103 31.67266 8 GTATCAA 11100 0.0 31.479559 1 TACCTGG 1010 0.0 29.641891 2 TATCAAC 13150 0.0 26.5251 2 GGTATCA 7300 0.0 26.310118 1 ATCAACG 13230 0.0 26.291523 3 TCAACGC 13315 0.0 26.129026 4 GTACATG 8030 0.0 25.872011 1 CAACGCA 13475 0.0 25.748232 5 TACATGG 7975 0.0 25.56309 2 AACGCAG 13735 0.0 25.318388 6 CATGGGT 795 0.0 24.511507 4 GAGTACT 6985 0.0 24.483164 12-13 ACATGGG 7890 0.0 24.211048 3 GTACCTG 1260 0.0 24.147587 1 TATCACG 100 0.0016491965 23.760565 2 CGGGTCG 100 0.0016521417 23.753283 6 CGCAGAG 15000 0.0 23.026024 8 ACGCAGA 15090 0.0 22.95166 7 >>END_MODULE