FastQCFastQC Report
Thu 26 May 2016
SRR937390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981115
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109731.1184213879106935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.9025445539004093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64330.6556825652446452No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28130.28671460532149645No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26380.268877756430184No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.2244385214781142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.2188326546837017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.18285318234865433No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.13403117881186202No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12670.12913878597310202No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.10742879275110462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC459.6244336E-442.222183
GTACGTG851.6291271E-533.537921
TACCTGG10350.033.043442
GTACCTG13100.026.8388731
GGTATCA97350.025.1835861
GTATCAA133050.025.0326581
GTACATG88950.023.8227981
GAGTACT78250.023.70323812-13
TACATGG89900.023.4593732
ACCTGGG14600.022.7739493
ACATGGG89900.022.7196643
GTACTTT85100.021.6278414-15
AGTACTT81000.021.46184712-13
TATCAAC157250.021.174862
CATGGGG25050.021.0478824
ATGGGAG18750.021.0266445
ATCAACG158600.020.994623
TCAACGC159300.020.9023654
CAACGCA160400.020.7886315
AACGCAG163750.020.3332926
Produced by FastQC (version 0.11.5)