FastQCFastQC Report
Thu 26 May 2016
SRR937389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087715
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107270.986195832548048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.7361303282569424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.529918223063946No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25330.23287350087109215No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23300.2142105238964251No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.1743103662264472No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17360.15960063067991154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16730.15380867230846315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.14314411403722482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14820.13624892549978623No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.1105068882933489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12250.034.5306322
GTATCAA118400.030.2810211
GTACCTG15050.029.0671311
ACGTATG700.00838789527.145168
GGTATCA79150.027.0942081
TATCACG1257.3565097E-626.6157462
TATCAAC142900.024.9780372
ATCAACG142950.024.9016593
TCAACGC144150.024.694364
CAACGCA145750.024.3895425
AACGCAG149650.023.7758246
TATAACG1401.7712311E-523.7640572
AGTACTT77650.023.73453512-13
GTACATG88300.023.6403921
GAGTACT76500.023.5325112-13
TACATGG88200.023.1174182
ACCTGGG17700.023.0916943
ATAACGC1503.0202595E-522.1787663
ACATGGG87750.022.1516863
GGACCGA2154.417052E-822.0948986
Produced by FastQC (version 0.11.5)