FastQCFastQC Report
Thu 26 May 2016
SRR937389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087715
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121031.1126995582482544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.8896631930239078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71490.657249371388645No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29480.27102687744491893No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28790.26468330399047546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.21724440685289806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23610.21706053515856638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.1909507545634656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.14452315174471256No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14310.13156019729432802No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.11326496370832433No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.10967946566885628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG600.003948314631.6708375
TACCTGG13850.029.8414752
GTACCTG14900.027.7589721
TATCACG700.0083859927.146432
GTATCAA150400.026.1097431
GGTATCA107300.023.615541
GAGTACT87700.023.3707212-13
GTACATG98300.022.5373481
AGTACTT90050.022.41796112-13
TACATGG98550.022.12622
TATCAAC177900.021.9506112
ATCAACG178300.021.9013673
TCAACGC179900.021.7065814
ACCTGGG17750.021.678913
CAACGCA181950.021.4881275
ACATGGG96900.021.4734133
GTACTTT95300.021.33151814-15
AACGCAG184400.021.301766
ATGGGAG20550.021.036835
CATGGGG24350.020.680344
Produced by FastQC (version 0.11.5)