##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937389_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1087715 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15842752927008 33.0 31.0 34.0 31.0 34.0 2 32.36028739145824 34.0 31.0 34.0 31.0 34.0 3 32.426027957691126 34.0 31.0 34.0 31.0 34.0 4 36.05100049185678 37.0 35.0 37.0 35.0 37.0 5 35.961936720556395 37.0 35.0 37.0 35.0 37.0 6 36.06013983442354 37.0 35.0 37.0 35.0 37.0 7 36.01569069103579 37.0 35.0 37.0 35.0 37.0 8 36.00716088313575 37.0 35.0 37.0 35.0 37.0 9 37.68317528028941 39.0 37.0 39.0 35.0 39.0 10-11 37.62403892563769 39.0 37.0 39.0 35.0 39.0 12-13 37.54326500967625 39.0 37.0 39.0 35.0 39.0 14-15 38.67917101446611 40.0 38.0 41.0 34.0 41.0 16-17 38.677888509398144 40.0 38.0 41.0 34.5 41.0 18-19 38.76048275513347 40.0 38.0 41.0 35.0 41.0 20-21 38.69954124012264 40.0 38.0 41.0 34.0 41.0 22-23 38.57630537410995 40.0 38.0 41.0 34.0 41.0 24-25 38.44156419650368 40.0 38.0 41.0 34.0 41.0 26-27 38.35314029869957 40.0 38.0 41.0 34.0 41.0 28-29 38.23694304114589 40.0 38.0 41.0 34.0 41.0 30-31 38.19557972446827 40.0 37.5 41.0 34.0 41.0 32-33 38.027230938251286 40.0 37.0 41.0 34.0 41.0 34-35 37.88463843929706 40.0 37.0 41.0 33.0 41.0 36-37 37.69171520113265 40.0 36.0 41.0 33.0 41.0 38-39 37.52329286623794 40.0 36.0 41.0 33.0 41.0 40-41 37.31269542113513 40.0 36.0 41.0 33.0 41.0 42-43 37.038019609916205 40.0 36.0 41.0 32.5 41.0 44-45 36.617552391940904 40.0 35.5 41.0 31.0 41.0 46-47 36.05332922686549 39.0 35.0 41.0 29.5 41.0 48-49 35.58538036158369 39.0 35.0 41.0 27.5 41.0 50-51 35.30373535347035 39.0 35.0 41.0 26.0 41.0 52-53 35.04390810092717 39.0 35.0 40.5 25.0 41.0 54-55 34.81040024271064 39.0 35.0 40.0 24.0 41.0 56-57 34.57605438924718 38.0 34.5 40.0 23.5 41.0 58-59 34.347921560335195 38.0 34.0 40.0 23.0 41.0 60-61 34.031105574530095 38.0 34.0 40.0 22.0 41.0 62-63 33.66226125409689 37.5 34.0 40.0 21.5 41.0 64-65 33.30467585718685 37.0 33.0 40.0 19.5 41.0 66-67 32.881015707239484 36.0 33.0 39.0 18.0 41.0 68-69 32.4999563304726 36.0 32.5 39.0 14.5 40.0 70-71 32.10295481812791 35.0 32.0 38.5 10.5 40.0 72-73 31.659443880060493 35.0 31.5 38.0 7.5 40.0 74-75 31.219837917101447 35.0 31.0 37.0 6.5 39.0 76-77 29.872214228911066 33.5 29.5 36.0 2.0 38.5 78-79 30.466623610044913 34.5 31.0 36.0 2.0 39.0 80-81 30.32379115852958 35.0 31.0 36.0 2.0 37.5 82-83 29.987666346423467 34.0 31.0 35.5 2.0 37.0 84-85 29.62684296897625 34.0 30.0 35.0 2.0 37.0 86-87 29.242682136405215 34.0 30.0 35.0 2.0 36.0 88-89 28.832027691077165 34.0 29.0 35.0 2.0 36.0 90-91 28.387138174981498 34.0 29.0 35.0 2.0 35.5 92-93 28.038018690557728 34.0 29.0 35.0 2.0 35.0 94-95 27.659721526318936 34.0 28.0 35.0 2.0 35.0 96-97 27.191594305493627 33.0 27.0 35.0 2.0 35.0 98-99 26.742416901486145 33.0 26.5 35.0 2.0 35.0 100-101 24.947010476089787 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02266643280580638 1101 2 0.11056122595726237 1101 3 0.13803929933859393 1101 4 0.019869978647484743 1101 5 -0.040660465989127204 1101 6 0.06466037002812186 1101 7 0.023151807807671787 1101 8 0.07971330929805731 1101 9 0.16096724917240124 1101 10-11 0.03706615721284834 1101 12-13 0.07573507831155268 1101 14-15 -0.03273558868107074 1101 16-17 0.05896071298699468 1101 18-19 0.08591410029276858 1101 20-21 0.08595792298559957 1101 22-23 0.16250428635414949 1101 24-25 0.08730466071565957 1101 26-27 0.0659085600889 1101 28-29 -0.09041328780937619 1101 30-31 0.07469153641516613 1101 32-33 0.1298862755139183 1101 34-35 0.16821790474740794 1101 36-37 0.04593507926595208 1101 38-39 -0.09607343065057705 1101 40-41 -0.056147704428454404 1101 42-43 0.08982746112635454 1101 44-45 0.1168229719724323 1101 46-47 0.15275124755893899 1101 48-49 0.25486605898039016 1101 50-51 0.09956800257387499 1101 52-53 0.21458293097363423 1101 54-55 0.2647223706272399 1101 56-57 0.2013379578647374 1101 58-59 0.0957100319969797 1101 60-61 0.33537364396131863 1101 62-63 0.1482740581571349 1101 64-65 0.23891227417802696 1101 66-67 0.225863384911122 1101 68-69 0.311715181700869 1101 70-71 0.17310070554601964 1101 72-73 0.2987096177792097 1101 74-75 0.352427356658783 1101 76-77 0.289777183445473 1101 78-79 0.43996704634718853 1101 80-81 0.49601035298794827 1101 82-83 0.30651393431556784 1101 84-85 0.4095267457743539 1101 86-87 0.37271330546176173 1101 88-89 0.28868908498752077 1101 90-91 0.43422884709534415 1101 92-93 0.26435210773193063 1101 94-95 0.2317580710130258 1101 96-97 0.3043747925909983 1101 98-99 0.25702573344252855 1101 100-101 0.6721805913632046 1102 1 0.06496586013483352 1102 2 0.1288952587244978 1102 3 0.12401537934777451 1102 4 0.09625140384513742 1102 5 -0.031122436135518683 1102 6 0.024773178878994884 1102 7 0.04785274305998399 1102 8 0.09029627394215822 1102 9 0.1302094541599388 1102 10-11 0.01661533692931272 1102 12-13 0.06649963199596698 1102 14-15 -0.04324525870610074 1102 16-17 0.052705373992885995 1102 18-19 0.037560083390900445 1102 20-21 0.005943873769233221 1102 22-23 0.1323977107890144 1102 24-25 0.0548928720869597 1102 26-27 -0.04004821684184634 1102 28-29 4.4090584278677625E-4 1102 30-31 0.09832321344144646 1102 32-33 0.05080397603469322 1102 34-35 0.12407800412972847 1102 36-37 -0.04514451155548471 1102 38-39 -0.27894415386091254 1102 40-41 -0.21673556619091272 1102 42-43 -0.12594768136032286 1102 44-45 -0.0683545858638368 1102 46-47 -0.23626545647933028 1102 48-49 -0.17218536335682444 1102 50-51 -0.18747577896145629 1102 52-53 -0.05187998097617452 1102 54-55 -0.013093748091876023 1102 56-57 0.13037287529154185 1102 58-59 0.08748676491203611 1102 60-61 0.07241265810328912 1102 62-63 -0.030345901459476465 1102 64-65 0.0498977754645793 1102 66-67 0.07661964916871256 1102 68-69 0.12810956642497473 1102 70-71 0.09116899667225198 1102 72-73 0.03235437757069093 1102 74-75 -0.20373902342376127 1102 76-77 -0.04352263759890107 1102 78-79 0.04134193664849306 1102 80-81 0.06118451202948805 1102 82-83 -0.011380071287256044 1102 84-85 -0.09909412840343279 1102 86-87 0.10576651930108127 1102 88-89 0.10854745124397525 1102 90-91 0.22581430051337392 1102 92-93 0.2996924379987824 1102 94-95 0.3212650706491509 1102 96-97 0.14633764843475205 1102 98-99 0.0127273088876656 1102 100-101 0.33703089196843905 1103 1 0.07656418678490695 1103 2 0.15735247918141226 1103 3 0.23051801930274252 1103 4 0.07343226810689885 1103 5 -0.005285827870679327 1103 6 -0.04699584440436411 1103 7 0.010355777874309524 1103 8 -0.078119246164988 1103 9 -0.17205671839893455 1103 10-11 0.0085433978230256 1103 12-13 -0.016659616383904563 1103 14-15 0.032614427487423825 1103 16-17 0.031849196178121986 1103 18-19 0.09632909600238548 1103 20-21 -0.02592267710759444 1103 22-23 0.02579835697101629 1103 24-25 0.05689451555564062 1103 26-27 0.13149282852161548 1103 28-29 -0.07686548634326584 1103 30-31 -0.03862194913121897 1103 32-33 -0.05921704755815682 1103 34-35 -0.1367349392533157 1103 36-37 -0.2626002966452745 1103 38-39 -0.08969546716846821 1103 40-41 -0.08942743982524348 1103 42-43 -0.15873901686538971 1103 44-45 -0.11662214043045793 1103 46-47 0.1583351085964182 1103 48-49 0.3110474542581798 1103 50-51 0.20486535017622742 1103 52-53 0.37331684840989254 1103 54-55 0.17280763845141678 1103 56-57 0.24954236333503843 1103 58-59 0.0927790141963527 1103 60-61 0.027414032107138553 1103 62-63 0.1397257601780737 1103 64-65 0.23746697590105015 1103 66-67 0.2925880417442883 1103 68-69 0.5039513105294304 1103 70-71 0.3676742645763227 1103 72-73 0.4166775939669556 1103 74-75 0.41432692609293653 1103 76-77 0.23510633333891562 1103 78-79 0.3578734040045646 1103 80-81 0.38125570677252085 1103 82-83 0.4640899580870794 1103 84-85 0.5892221032509433 1103 86-87 0.20427192327155197 1103 88-89 0.3001636266042169 1103 90-91 0.2730200035631256 1103 92-93 0.36681519390366546 1103 94-95 0.47131029271751856 1103 96-97 0.5740717159930533 1103 98-99 0.5920303421012072 1103 100-101 0.6716675279801443 1104 1 0.2066872727821334 1104 2 0.18653883883155942 1104 3 0.20361138926696043 1104 4 0.08802253763884238 1104 5 0.06016084391809784 1104 6 0.10165610782026135 1104 7 0.06685814879310925 1104 8 0.1058268008808696 1104 9 0.1959711893719387 1104 10-11 0.13938241592892808 1104 12-13 0.14861398997372532 1104 14-15 0.10248086443495907 1104 16-17 0.14311746311796725 1104 18-19 0.1502616780920505 1104 20-21 0.15868400647757852 1104 22-23 -0.020706186966648943 1104 24-25 0.03433586344819872 1104 26-27 0.10567658830990467 1104 28-29 0.06477459964619214 1104 30-31 0.19902385655432653 1104 32-33 0.17939788573793436 1104 34-35 0.03858596906857059 1104 36-37 0.07415263110960524 1104 38-39 0.22898454028187842 1104 40-41 0.11348401741673086 1104 42-43 0.06368692587556524 1104 44-45 0.15951423202496784 1104 46-47 0.24691667053134125 1104 48-49 0.11004029881748068 1104 50-51 -0.17437397680878775 1104 52-53 -0.009491468043741236 1104 54-55 -0.024009664987381996 1104 56-57 0.10573701926078627 1104 58-59 0.253756057804182 1104 60-61 0.2582293798289541 1104 62-63 0.09862660067071971 1104 64-65 0.10488857260427409 1104 66-67 0.3598575271268558 1104 68-69 0.30668097146088513 1104 70-71 0.27044816196327304 1104 72-73 0.1417761558941173 1104 74-75 -0.004026740488360048 1104 76-77 0.12090740122247112 1104 78-79 0.07358918089761346 1104 80-81 0.1382569019328983 1104 82-83 0.1751968007682727 1104 84-85 0.0348452164109041 1104 86-87 0.1177430880872734 1104 88-89 0.15316666106984655 1104 90-91 0.2651622121263735 1104 92-93 0.2846304562568882 1104 94-95 0.07615069224256032 1104 96-97 0.2979663866642852 1104 98-99 0.4117890982031227 1104 100-101 1.0792793026380885 1105 1 0.07016985486268368 1105 2 0.1020257704350982 1105 3 0.0782370151370273 1105 4 0.06745831016964132 1105 5 -0.020140450294569234 1105 6 -0.01461014810450223 1105 7 -0.0863978364969924 1105 8 -0.011518487379383657 1105 9 -0.017954797775679765 1105 10-11 0.08300662883014809 1105 12-13 0.01937847499145562 1105 14-15 0.0331045117312172 1105 16-17 0.07298036131115992 1105 18-19 0.059928722524389855 1105 20-21 0.11500928567980395 1105 22-23 0.12312444043743653 1105 24-25 0.12337064084820781 1105 26-27 0.10966295535204296 1105 28-29 0.14679671041711373 1105 30-31 0.21715660883814536 1105 32-33 0.1676391465152065 1105 34-35 0.08946745708963988 1105 36-37 0.15183669795535337 1105 38-39 0.13730331607064983 1105 40-41 0.2612383987680431 1105 42-43 0.09236789754731234 1105 44-45 0.017851061256102696 1105 46-47 -0.04734817868589403 1105 48-49 0.06680700033876974 1105 50-51 0.10119668937367265 1105 52-53 0.25450099333964005 1105 54-55 0.2726918771731448 1105 56-57 0.28202523151082914 1105 58-59 0.3867587939964281 1105 60-61 0.32571690416979493 1105 62-63 0.33033261966753713 1105 64-65 0.09252561701574535 1105 66-67 0.2638726961926494 1105 68-69 0.3974710026213373 1105 70-71 0.3344742257075666 1105 72-73 0.23184718001476412 1105 74-75 0.20735611145462585 1105 76-77 0.13876569944029526 1105 78-79 0.2193263770414795 1105 80-81 0.16833205851781585 1105 82-83 0.14777226520090636 1105 84-85 0.2915265554128119 1105 86-87 0.34494722556058477 1105 88-89 0.32625638772194776 1105 90-91 0.3309090924289215 1105 92-93 0.22688135527657494 1105 94-95 0.2617359055340671 1105 96-97 0.2948419932286406 1105 98-99 0.34476699786772613 1105 100-101 0.6726688207885836 1106 1 -0.10358401542041662 1106 2 0.041605879395106626 1106 3 -0.03195353652789379 1106 4 -0.009770968754899911 1106 5 -0.13161950915434062 1106 6 -0.1150747890987418 1106 7 -0.08870075327648408 1106 8 -0.07124029900725048 1106 9 -0.11145023790108866 1106 10-11 -0.3563933706729969 1106 12-13 -0.42151829620494397 1106 14-15 -0.24566269493258375 1106 16-17 -0.19833611046465904 1106 18-19 -0.2176187083694714 1106 20-21 -0.09740683920933435 1106 22-23 -0.007009817706205013 1106 24-25 -0.029635034048837383 1106 26-27 0.0656686327333631 1106 28-29 -0.08419014863130059 1106 30-31 -0.1032941368150091 1106 32-33 -0.0686586642646958 1106 34-35 -0.1499220256773981 1106 36-37 -0.00563320720356586 1106 38-39 0.15808711930334596 1106 40-41 -0.021607148470728532 1106 42-43 -0.44796502753791856 1106 44-45 -0.4988055441236625 1106 46-47 -0.301282638717737 1106 48-49 -0.31216275700617757 1106 50-51 -0.4610557636343202 1106 52-53 -0.4875938576829313 1106 54-55 -0.7973533116685161 1106 56-57 -0.6217491174245424 1106 58-59 -0.5823381496081979 1106 60-61 -0.44776833389349946 1106 62-63 -0.5345981230707437 1106 64-65 -0.5587091885778293 1106 66-67 -0.7728108386562553 1106 68-69 -0.3108117137439521 1106 70-71 -0.3301358807930157 1106 72-73 -0.47299482465731657 1106 74-75 -0.5406885623153919 1106 76-77 -0.6049108024448522 1106 78-79 -0.4798481833879329 1106 80-81 -0.43172060797164136 1106 82-83 -0.7511026924630215 1106 84-85 -0.8112082928273772 1106 86-87 -0.5603458394386429 1106 88-89 -0.6403538032437623 1106 90-91 -0.5662715942062952 1106 92-93 -0.5667132071568162 1106 94-95 -1.0211564287590242 1106 96-97 -0.8037946402896061 1106 98-99 -0.6956872423229363 1106 100-101 -0.24258119183978266 1107 1 0.05652025905768454 1107 2 0.01657324390937731 1107 3 -0.023735260059346786 1107 4 -0.015904352197246396 1107 5 -0.07973977477190175 1107 6 -0.01076054193203646 1107 7 -0.095319319263794 1107 8 -0.04548287238365134 1107 9 -0.00768281532399584 1107 10-11 0.10609744515946318 1107 12-13 -0.09553677092225854 1107 14-15 0.0010635656720907605 1107 16-17 0.014757455199536196 1107 18-19 0.09535137436011354 1107 20-21 0.1378818394337813 1107 22-23 0.12548031210995703 1107 24-25 0.17372654526011644 1107 26-27 0.020633650873023157 1107 28-29 0.06120504149961192 1107 30-31 -0.12982904858237276 1107 32-33 -0.14358009551342832 1107 34-35 -0.025942972041860912 1107 36-37 -0.08451166002801358 1107 38-39 -0.005095280500988508 1107 40-41 0.04373292174154386 1107 42-43 -0.21646136928141146 1107 44-45 0.03007922203922675 1107 46-47 0.12451177313264594 1107 48-49 0.20337252958459118 1107 50-51 0.2098477993752823 1107 52-53 0.5015442175144003 1107 54-55 0.1571995995758897 1107 56-57 0.3858527385975421 1107 58-59 0.3157566140989587 1107 60-61 0.4738612630371293 1107 62-63 0.49178417492479554 1107 64-65 0.35493179821187937 1107 66-67 0.4570950975720649 1107 68-69 0.3731306330482269 1107 70-71 0.301441549071761 1107 72-73 0.2991975541986669 1107 74-75 0.1331400151353499 1107 76-77 0.35490703537095314 1107 78-79 0.27490148528607605 1107 80-81 0.30982622954291017 1107 82-83 0.49537451439674385 1107 84-85 0.5318281942919221 1107 86-87 0.619115031285066 1107 88-89 0.4477222391778959 1107 90-91 0.4546707933491696 1107 92-93 0.525774218363189 1107 94-95 0.6388240645165233 1107 96-97 0.5930732478837726 1107 98-99 0.6454905383049585 1107 100-101 1.3538458562917164 1108 1 -0.04352689726920289 1108 2 -0.027730898924616554 1108 3 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-0.5306185476942176 2316 72-73 -0.4988978595154059 2316 74-75 -0.8249683065632318 2316 76-77 -0.44650757436008703 2316 78-79 -0.8986296826669964 2316 80-81 -0.803472067832466 2316 82-83 -0.7497674295281591 2316 84-85 -0.8271828082282084 2316 86-87 -0.9486474969237086 2316 88-89 -0.9296914222114268 2316 90-91 -0.8248968330744404 2316 92-93 -0.7616216177141837 2316 94-95 -0.6971852608437139 2316 96-97 -0.615017511931125 2316 98-99 -0.5026944410159224 2316 100-101 -1.088972650421315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 23.0 6 52.0 7 145.0 8 294.0 9 616.0 10 1110.0 11 1922.0 12 3164.0 13 4632.0 14 5860.0 15 8561.0 16 12264.0 17 18480.0 18 16467.0 19 8151.0 20 6182.0 21 6003.0 22 6155.0 23 6284.0 24 7087.0 25 8080.0 26 9759.0 27 11792.0 28 14371.0 29 17716.0 30 22265.0 31 28360.0 32 36220.0 33 47226.0 34 65575.0 35 96235.0 36 145273.0 37 230857.0 38 215702.0 39 24815.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.83348956670766 20.01887679396237 15.375203877654089 19.772429761675877 2 15.220714985083408 22.20452967918986 40.425754908225045 22.14900042750169 3 19.395429869037386 28.19497754466933 30.578874061679763 21.830718524613523 4 12.190049783261241 17.888049718906146 37.77607185705815 32.14582864077447 5 12.152425299024584 39.7576723896956 33.22207264862956 14.867829662650248 6 28.753395880354688 37.959851615542675 18.07569078297164 15.211061721130994 7 26.62471327507665 35.620727856102015 20.6511816054757 17.10337726334564 8 26.955774260720872 34.359000289597915 19.554203077092804 19.131022372588408 9 27.73888380687956 16.611704352702684 20.74817392423567 34.90123791618209 10-11 24.34314135596181 28.449410001700816 26.404113209802198 20.803335432535178 12-13 26.481616749648452 26.112990989607777 27.79656123972994 19.608831021013827 14-15 23.20051667946107 26.28841194614398 28.205963878405647 22.305107495989297 16-17 20.070376891005456 30.054793764910848 28.596599293013337 21.278230051070363 18-19 19.962674046050665 27.24316571896131 33.21738690741601 19.57677332757202 20-21 21.492072831578124 26.016879421539652 33.2025852360223 19.288462510859922 22-23 20.855195112645433 26.2762421796351 32.998653127456436 19.86990958026303 24-25 20.43413985661672 26.498096006854745 33.51083097211215 19.55693316441638 26-27 20.475194711048943 26.481116372460644 33.45485870893807 19.58883020755235 28-29 20.06285833017678 26.52746569775781 33.84075705811615 19.568918913949272 30-31 20.088810028362207 26.785279232151805 33.64295794394672 19.482952795539273 32-33 19.853730067159137 26.898130484547885 33.33216881260256 19.915970635690417 34-35 20.295527780714618 26.57695260247399 33.27640972129648 19.85110989551491 36-37 20.617808892954496 26.532731459987218 33.11124697186303 19.73821267519525 38-39 20.562141737495576 26.560542053754887 33.26165401782636 19.615662190923175 40-41 20.33441664406577 26.888339316824723 33.25554028398983 19.521703755119677 42-43 20.19237488552398 27.175228308403 32.63141295592719 20.000983850145833 44-45 20.304583461660453 27.434943896149267 32.14444960306698 20.1160230391233 46-47 20.6153725930046 27.629112405363536 31.260256592949442 20.49525840868242 48-49 20.871505863208654 27.911263520315522 30.763205435247283 20.454025181228538 50-51 21.219850788120052 28.2454043568398 30.314604469001534 20.220140386038622 52-53 20.967762695191293 28.7073360209246 30.045048565111266 20.27985271877284 54-55 20.63454121713868 28.71505863208653 30.129859384121758 20.520540766653028 56-57 20.896880157026427 29.052371255338027 29.59497662531086 20.45577196232469 58-59 20.92128912444896 28.35365881687758 30.00096532639524 20.724086732278217 60-61 20.826227458479472 28.41226791944581 30.355791728531827 20.405712893542884 62-63 21.12722542210046 28.346763628340142 30.379878920489283 20.146132029070117 64-65 20.92082944521313 28.295693265239514 30.47604381662476 20.307433472922597 66-67 20.664742142932663 28.647991431579044 30.25640907774555 20.430857347742744 68-69 20.678164776618875 28.89047222847897 29.99259916430315 20.438763830599008 70-71 20.729556915184585 28.8249679373733 29.72437633019679 20.721098817245327 72-73 20.734301142441332 28.74642197010337 29.505352737322156 21.013924150133146 74-75 20.714525404172967 29.04639542527224 29.314296483913527 20.92478268664126 76-77 20.867092942544694 28.686098840229292 29.75347402582478 20.69333419140124 78-79 20.67039619753336 28.68154801579458 29.624488032251094 21.023567754420966 80-81 20.563888518591728 29.39110888422059 29.338429643794562 20.706572953393124 82-83 20.534093092571293 29.286677643827 29.182238329412264 20.99699093418944 84-85 20.539561284675855 28.826554417423576 29.157988174112585 21.47589612378798 86-87 20.671269588081437 28.66853909342061 29.3714346129271 21.28875670557085 88-89 20.81910243032412 28.416496968415437 29.365918462097145 21.398482139163292 90-91 20.56452558308244 28.534663597533477 29.39867332166548 21.502137497718607 92-93 20.28676118725806 28.834434510634598 29.27508930545615 21.60371499665119 94-95 20.119194825850524 28.66256326335483 29.2441034646024 21.97413844619225 96-97 20.22294442937718 28.567731437003257 28.960113632707095 22.249210500912465 98-99 20.158249391958453 28.688069054948752 28.86748210811027 22.286199444982525 100-101 19.99501641731896 29.18333607644926 29.012864447470555 21.80878305876122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1775.0 1 1221.5 2 998.5 3 1312.5 4 1066.5 5 906.0 6 1390.5 7 2660.5 8 3979.5 9 4473.0 10 3933.0 11 3369.5 12 3464.0 13 3606.0 14 3781.0 15 3958.0 16 3994.0 17 4009.5 18 4079.0 19 4239.5 20 4602.5 21 5009.5 22 5449.5 23 6065.5 24 6774.0 25 7729.5 26 9029.5 27 10924.5 28 12525.5 29 14326.0 30 16713.5 31 18115.5 32 19756.0 33 21760.0 34 24252.0 35 26589.5 36 29072.5 37 31921.5 38 33279.5 39 33668.0 40 33947.5 41 34795.5 42 35172.5 43 36104.5 44 38337.0 45 39339.5 46 38730.0 47 39485.0 48 46987.5 49 45841.0 50 37379.5 51 33663.0 52 29735.0 53 27780.5 54 26385.0 55 24249.0 56 21130.0 57 18157.0 58 16477.5 59 15140.0 60 13010.0 61 10681.0 62 8489.5 63 6146.5 64 4317.0 65 3169.0 66 2198.0 67 1634.5 68 1195.5 69 803.5 70 580.5 71 421.0 72 333.5 73 298.5 74 239.5 75 174.0 76 116.5 77 67.0 78 41.0 79 25.0 80 16.5 81 11.5 82 7.5 83 3.0 84 2.0 85 1.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06122927421245455 2 0.0 3 0.0 4 0.0 5 0.016456516642686735 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024362999498949633 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.193584716584767E-4 24-25 9.193584716584767E-5 26-27 0.0012411339367389435 28-29 0.002850011262141278 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013882312922042998 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.596792358292383E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8387169433169533E-4 84-85 0.0015169414782364866 86-87 0.0 88-89 0.0 90-91 0.010250846958992015 92-93 0.001425005631070639 94-95 0.0 96-97 0.0 98-99 1.379037707487715E-4 100-101 0.013238761991882066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1087715.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.69772451877461 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20569005239167 42.77673290286392 2 12.44361774751939 13.612751511415006 3 3.9113590059417764 6.418273122030942 4 1.6768257344718418 3.6687420836053084 5 0.9362861351802803 2.5606360546419564 6 0.5806024622973065 1.905458012259618 7 0.3924028175814199 1.5024478851521634 8 0.27379245103650174 1.1980659249691727 9 0.21419214500502382 1.0544240647413197 >10 1.2285822594950062 12.507823328804873 >50 0.09217772745121817 3.4802457324348017 >100 0.03958634015386784 3.872538619006239 >500 0.0028636918990525375 1.1126785952576144 >1k 0.0015160721818513435 1.6636737923129747 >5k 3.3690492930029856E-4 1.5503420042898821 >10k+ 1.6845246465014928E-4 1.1151663662142188 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12103 1.1126995582482544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9677 0.8896631930239078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7149 0.657249371388645 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2948 0.27102687744491893 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2879 0.26468330399047546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2363 0.21724440685289806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2361 0.21706053515856638 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2077 0.1909507545634656 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1572 0.14452315174471256 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1431 0.13156019729432802 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.11326496370832433 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.10967946566885628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.379037707487715E-4 0.0 24-25 0.0 0.0 0.0 5.51615082995086E-4 0.0 26-27 0.0 0.0 0.0 0.0010572622424072481 0.0 28-29 0.0 0.0 0.0 0.002574203720643735 0.0 30-31 0.0 0.0 0.0 0.005056471594121622 0.0 32-33 0.0 0.0 0.0 0.009607296028831081 0.0 34-35 0.0 0.0 0.0 0.012825050679635751 0.0 36-37 0.0 0.0 0.0 0.01765168265584275 0.0 38-39 0.0 0.0 0.0 0.02519042212344226 0.0 40-41 0.0 0.0 0.0 0.033740455909866095 0.0 42-43 0.0 0.0 0.0 0.04187677838404361 0.0 44-45 0.0 0.0 0.0 0.045921955659340916 0.0 46-47 0.0 0.0 0.0 0.05194375364870393 0.0 48-49 0.0 0.0 0.0 0.06270024776710811 0.0 50-51 0.0 0.0 0.0 0.07253738341385382 0.0 52-53 0.0 0.0 0.0 0.09529150558740111 0.0 54-55 0.0 0.0 0.0 0.10944962605094165 0.0 56-57 0.0 0.0 0.0 0.14990139880391462 0.0 58-59 0.0 0.0 0.0 0.23236785371167998 0.0 60-61 0.0 0.0 0.0 0.3038020069595436 0.0 62-63 0.0 0.0 0.0 0.3404844099787169 0.0 64-65 0.0 0.0 0.0 0.4062185407022979 0.0 66-67 0.0 0.0 0.0 0.538376321003204 0.0 68-69 0.0 0.0 0.0 0.7171915437407776 0.0 70-71 0.0 0.0 0.0 0.8979374192688342 0.0 72-73 0.0 0.0 0.0 1.104425332003328 0.0 74-75 0.0 0.0 0.0 1.3557779381547554 0.0 76-77 0.0 0.0 0.0 1.519147938568467 0.0 78-79 0.0 0.0 0.0 1.7390584849891746 0.0 80-81 0.0 0.0 0.0 2.0504911672634836 0.0 82-83 0.0 0.0 0.0 2.3308955011193193 0.0 84-85 0.0 0.0 0.0 2.6740000827422623 0.0 86-87 0.0 0.0 0.0 3.1201647490381212 0.0 88-89 0.0 0.0 0.0 3.714621936812492 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATATCG 60 0.0039483146 31.670837 5 TACCTGG 1385 0.0 29.841475 2 GTACCTG 1490 0.0 27.758972 1 TATCACG 70 0.00838599 27.14643 2 GTATCAA 15040 0.0 26.109743 1 GGTATCA 10730 0.0 23.61554 1 GAGTACT 8770 0.0 23.37072 12-13 GTACATG 9830 0.0 22.537348 1 AGTACTT 9005 0.0 22.417961 12-13 TACATGG 9855 0.0 22.1262 2 TATCAAC 17790 0.0 21.950611 2 ATCAACG 17830 0.0 21.901367 3 TCAACGC 17990 0.0 21.706581 4 ACCTGGG 1775 0.0 21.67891 3 CAACGCA 18195 0.0 21.488127 5 ACATGGG 9690 0.0 21.473413 3 GTACTTT 9530 0.0 21.331518 14-15 AACGCAG 18440 0.0 21.30176 6 ATGGGAG 2055 0.0 21.03683 5 CATGGGG 2435 0.0 20.68034 4 >>END_MODULE