FastQCFastQC Report
Thu 26 May 2016
SRR937388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170862
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110320.9422118063443856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83960.7170785284687692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.504927139150472No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28190.2407627884413364No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24990.2134324967417168No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.1695332156992028No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18590.1587719133424776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.14698572504701665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16610.14186129535333797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.13690767998278192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128250.028.71471
TACCTGG12600.027.9015392
GTACATG102950.027.0015981
TACATGG102700.026.7839812
GGTATCA89000.026.428661
CATGGGG28400.025.9297874
ACATGGG103050.025.6326683
TATCAAC153500.023.9861372
ATCAACG154500.023.7386383
TCAACGC156000.023.5113894
CAACGCA157250.023.2932835
GAGTACT77150.023.15064812-13
ATGGGAT25300.023.0967865
AACGCAG160300.022.9656876
CATGGGA69700.022.8348224
GTACACG2303.610694E-922.7260951
GTACCTG15900.022.4142441
AGTACTT80300.021.65093812-13
GGGCGCG1553.90527E-521.4524827
GTACTTT84600.021.14004114-15
Produced by FastQC (version 0.11.5)