##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937388_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1170862 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73708430199289 33.0 31.0 34.0 30.0 34.0 2 31.800208735102856 33.0 31.0 34.0 30.0 34.0 3 31.82465055659847 33.0 31.0 34.0 30.0 34.0 4 35.46288033944222 37.0 35.0 37.0 33.0 37.0 5 35.48864170158396 37.0 35.0 37.0 33.0 37.0 6 35.537180299642486 37.0 35.0 37.0 33.0 37.0 7 35.43365315468433 37.0 35.0 37.0 33.0 37.0 8 35.45134097784367 37.0 35.0 37.0 33.0 37.0 9 37.05340851441075 39.0 37.0 39.0 33.0 39.0 10-11 36.94705695461976 39.0 37.0 39.0 33.0 39.0 12-13 36.79492373994544 39.0 37.0 39.0 33.0 39.0 14-15 37.89830184940668 40.0 38.0 41.0 33.0 41.0 16-17 37.84777582669862 40.0 38.0 41.0 33.0 41.0 18-19 37.89063869183559 40.0 38.0 41.0 33.0 41.0 20-21 37.824096691155745 40.0 38.0 41.0 33.0 41.0 22-23 37.67329412005856 40.0 37.0 41.0 32.5 41.0 24-25 37.603944358942385 40.0 37.0 41.0 32.0 41.0 26-27 37.60308473586127 40.0 37.0 41.0 32.0 41.0 28-29 37.515917332700184 40.0 37.0 41.0 32.0 41.0 30-31 37.25504158474696 39.0 36.5 41.0 32.0 41.0 32-33 36.9660792646785 39.0 35.5 41.0 31.0 41.0 34-35 36.93622775356959 39.0 36.0 41.0 31.0 41.0 36-37 36.76441416665671 39.0 35.0 41.0 31.0 41.0 38-39 36.62294104685266 39.0 35.0 41.0 30.5 41.0 40-41 36.366834861836836 39.0 35.0 41.0 30.0 41.0 42-43 36.052895217369766 39.0 35.0 41.0 30.0 41.0 44-45 35.71583798944709 39.0 35.0 41.0 28.0 41.0 46-47 35.21648665683915 39.0 35.0 40.5 25.0 41.0 48-49 34.7620889566832 39.0 35.0 40.0 23.5 41.0 50-51 33.74774012650509 38.0 33.5 39.5 21.5 40.5 52-53 33.844546581919985 38.0 33.5 39.5 22.0 40.5 54-55 34.208027504522306 38.0 34.0 40.0 21.5 41.0 56-57 34.017375232948034 38.0 34.0 40.0 19.5 41.0 58-59 33.75729718788379 38.0 34.0 40.0 18.0 41.0 60-61 33.44993688410761 38.0 33.0 40.0 15.5 41.0 62-63 33.13922349516852 37.0 33.0 40.0 13.0 41.0 64-65 32.71819309192714 37.0 32.5 40.0 8.0 41.0 66-67 32.36622590877491 36.0 32.0 39.0 7.5 41.0 68-69 31.904099287533462 36.0 31.5 39.0 4.0 40.5 70-71 31.438999215962255 35.0 31.0 38.5 2.0 40.0 72-73 30.97416134437705 35.0 31.0 38.0 2.0 40.0 74-75 30.494608245890632 35.0 30.0 37.0 2.0 39.0 76-77 29.998598895514586 34.5 29.5 36.5 2.0 39.0 78-79 29.49935389482279 34.0 29.0 36.0 2.0 39.0 80-81 29.012083832253502 34.0 29.0 36.0 2.0 37.5 82-83 28.535453366835718 34.0 28.5 35.0 2.0 37.0 84-85 28.013208217535457 34.0 27.0 35.0 2.0 36.5 86-87 27.538771008026565 33.5 26.0 35.0 2.0 36.0 88-89 27.07314952573403 33.0 25.5 35.0 2.0 36.0 90-91 26.563815377046996 33.0 25.0 35.0 2.0 35.0 92-93 26.070862748983227 33.0 24.0 35.0 2.0 35.0 94-95 25.56696903648765 32.0 21.5 35.0 2.0 35.0 96-97 24.84827076119987 32.0 16.5 34.0 2.0 35.0 98-99 23.935240019746136 31.5 2.0 34.0 2.0 35.0 100-101 21.429559589430692 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06867262546088071 1101 2 0.04415089952154716 1101 3 -0.12290484487073527 1101 4 -0.15304969769304222 1101 5 -0.17720906168499795 1101 6 -0.06729183094403623 1101 7 -0.15152311816647313 1101 8 -0.3347086731147684 1101 9 -0.2607116252010613 1101 10-11 -0.22299545613410743 1101 12-13 -0.1465217724229504 1101 14-15 -0.30521306474572185 1101 16-17 -0.39291438868201567 1101 18-19 -0.02824809923929905 1101 20-21 -0.03960138560147897 1101 22-23 -0.17167217464609053 1101 24-25 -0.2628176157504143 1101 26-27 -0.08508790014779066 1101 28-29 -0.21018764143717306 1101 30-31 -0.16545080971778248 1101 32-33 -0.14977860067318005 1101 34-35 -0.18388501257255285 1101 36-37 -0.09040849114404637 1101 38-39 -0.20212978532241976 1101 40-41 -0.3615116577756936 1101 42-43 -0.19405463196910233 1101 44-45 -0.22269172505866663 1101 46-47 -0.3341757717286953 1101 48-49 -0.4011122194737595 1101 50-51 -0.3518113625167274 1101 52-53 -0.32735996445552473 1101 54-55 -0.3317198136307482 1101 56-57 -0.36959604066332474 1101 58-59 -0.3356446743978836 1101 60-61 -0.296671333333947 1101 62-63 -0.2596192755234057 1101 64-65 -0.218706076452456 1101 66-67 -0.23339901342629332 1101 68-69 -0.2647295975377624 1101 70-71 -0.1827935376183838 1101 72-73 0.024490343897756617 1101 74-75 -0.21420052384328514 1101 76-77 -0.1336411673963056 1101 78-79 -0.46443745249440127 1101 80-81 -0.2862463849462813 1101 82-83 -0.22524052033126907 1101 84-85 -0.31623933012858174 1101 86-87 -0.27446325507179736 1101 88-89 -0.3978598959179074 1101 90-91 -0.3558260535383866 1101 92-93 -0.33912509751023734 1101 94-95 -0.3753109732308211 1101 96-97 -0.12499964998034585 1101 98-99 -0.0644963525450919 1101 100-101 0.05908311941085742 1102 1 -0.1958460834066038 1102 2 -0.2762152376066247 1102 3 -0.17198541140152912 1102 4 -0.1310160196222867 1102 5 -0.15314642182089955 1102 6 -0.22533573082420588 1102 7 -0.22977819177409486 1102 8 -0.18034154460583807 1102 9 -0.21263206448861638 1102 10-11 -0.19125707477976306 1102 12-13 -0.21138454318781186 1102 14-15 -0.09369018839399956 1102 16-17 -0.004161266554490339 1102 18-19 -0.10588115360642547 1102 20-21 -0.22489815013891956 1102 22-23 -0.40157650158999303 1102 24-25 -0.2313968612422741 1102 26-27 -0.2289139080114566 1102 28-29 -0.1361136921323407 1102 30-31 -0.17989128115302577 1102 32-33 -0.2662913466535244 1102 34-35 0.02906213143272396 1102 36-37 -0.1404378475646837 1102 38-39 0.15672839429075225 1102 40-41 -0.08189151060859245 1102 42-43 -0.10580225337714211 1102 44-45 -0.11804017614654327 1102 46-47 0.01155479408021165 1102 48-49 0.030687521612236424 1102 50-51 0.02523961659208851 1102 52-53 0.07391391169888095 1102 54-55 0.0642679817983165 1102 56-57 0.14718452249869074 1102 58-59 -0.1253648740215425 1102 60-61 0.027632414181589127 1102 62-63 0.03214721099429596 1102 64-65 -0.07139627958925843 1102 66-67 -0.15806851306776082 1102 68-69 -0.04760469799928302 1102 70-71 -0.09843136601326208 1102 72-73 -0.12378075542800815 1102 74-75 -0.08758844728558657 1102 76-77 0.15755286891902998 1102 78-79 -0.082737811990242 1102 80-81 0.010514543101610485 1102 82-83 0.10356967837801534 1102 84-85 0.030805777696105707 1102 86-87 -0.3025174056365465 1102 88-89 -0.3718226758265679 1102 90-91 -0.3507454677574806 1102 92-93 -0.057960369490256625 1102 94-95 -0.034985116961781415 1102 96-97 -0.1739370438039458 1102 98-99 -0.18891536010557175 1102 100-101 -0.05759565878453898 1103 1 -0.00690504397394065 1103 2 -0.08541786135582186 1103 3 -0.19001643995021666 1103 4 -0.0672954926587579 1103 5 -0.1423177903817674 1103 6 -0.1265150475847321 1103 7 -0.1377245466970507 1103 8 -0.14828310320140758 1103 9 0.004072331529904716 1103 10-11 -0.019453418502507702 1103 12-13 -0.01254962908686963 1103 14-15 -0.24034738538865952 1103 16-17 -0.2698627821412387 1103 18-19 -0.43315828909213394 1103 20-21 -0.30978655646691067 1103 22-23 -0.10710150178764621 1103 24-25 -0.13800788699749944 1103 26-27 -0.22684911542626196 1103 28-29 -0.4310675340696406 1103 30-31 -0.41413863987877875 1103 32-33 -0.3324448238591131 1103 34-35 -0.12067947301785154 1103 36-37 -0.0671191316100419 1103 38-39 -0.22027347943934217 1103 40-41 -0.10325783143018441 1103 42-43 -0.28148645474910694 1103 44-45 -0.2306545171654122 1103 46-47 -0.20759362062816678 1103 48-49 -0.19616479630521155 1103 50-51 -0.06575251163324225 1103 52-53 -0.21165915718638928 1103 54-55 -0.1987958172619173 1103 56-57 -0.06766907092558938 1103 58-59 -0.15076696174163118 1103 60-61 -0.10456649109621452 1103 62-63 0.05173730947712585 1103 64-65 -0.16118590002056266 1103 66-67 0.12689131110204244 1103 68-69 0.16365850178356212 1103 70-71 0.013331231593316062 1103 72-73 -0.045908163803662916 1103 74-75 0.06386428648925246 1103 76-77 0.10072693080219608 1103 78-79 0.22368162420411153 1103 80-81 0.2956878301934296 1103 82-83 0.037805705121492394 1103 84-85 -0.09901841845787018 1103 86-87 0.2910117689471363 1103 88-89 0.5203782850201151 1103 90-91 0.468765306849388 1103 92-93 0.22997739420892316 1103 94-95 -0.2253118206462581 1103 96-97 -0.24671472348833845 1103 98-99 -0.18535553646284342 1103 100-101 0.3746749278695489 1104 1 0.04234507407344168 1104 2 -0.011791463020422555 1104 3 0.0014388320545926092 1104 4 -0.026535561544235975 1104 5 0.03722262333467796 1104 6 0.024596558073007202 1104 7 0.08825129726247383 1104 8 0.07006275747212243 1104 9 0.07111805665794435 1104 10-11 -0.007720171573900814 1104 12-13 0.09837109438360159 1104 14-15 0.20704874012035646 1104 16-17 0.10015940903728904 1104 18-19 0.14873171438443222 1104 20-21 0.14864317982762287 1104 22-23 -0.06982562606054898 1104 24-25 5.488965327131723E-4 1104 26-27 0.19310438053475565 1104 28-29 0.20894156379466722 1104 30-31 0.08207577222218987 1104 32-33 0.005377384961668952 1104 34-35 0.1328762179614884 1104 36-37 0.21436204451665475 1104 38-39 0.2102895791957593 1104 40-41 0.2570694841290617 1104 42-43 0.33726488931909415 1104 44-45 -0.005206499237203843 1104 46-47 -0.020445708841705823 1104 48-49 0.0593373072155714 1104 50-51 -0.028855137041645662 1104 52-53 0.04682973252246825 1104 54-55 -0.016123039997587796 1104 56-57 -0.19391808143195277 1104 58-59 -0.034947632181584254 1104 60-61 0.06913237881989431 1104 62-63 0.12082832444247771 1104 64-65 0.22409366328195546 1104 66-67 0.13209779168720814 1104 68-69 0.23308362399647464 1104 70-71 0.2401338396477506 1104 72-73 0.2753234148948849 1104 74-75 0.17590754112625362 1104 76-77 0.2756570104286844 1104 78-79 0.24643637449425526 1104 80-81 0.5061310022693277 1104 82-83 0.5486000501473285 1104 84-85 0.743598330260614 1104 86-87 0.7446213091360043 1104 88-89 0.5428254490866671 1104 90-91 0.8064482802943509 1104 92-93 0.8329723673217977 1104 94-95 0.8783182156817766 1104 96-97 0.8290503648122574 1104 98-99 0.9023468953420917 1104 100-101 1.0820416611654835 1105 1 0.1495105975902149 1105 2 0.06272002935632415 1105 3 0.05593123174002912 1105 4 0.10617570061441484 1105 5 -0.09477457452172189 1105 6 0.07005845591945814 1105 7 0.0814512170331696 1105 8 0.026026635464766912 1105 9 0.07043281373196919 1105 10-11 -0.028096590512014075 1105 12-13 -0.008633852893872529 1105 14-15 0.0011767670265854235 1105 16-17 -0.03848964482750716 1105 18-19 -0.050543104496746594 1105 20-21 1.1751086289990553E-4 1105 22-23 0.0840137635850482 1105 24-25 0.12569925294726403 1105 26-27 0.2316082687736909 1105 28-29 -0.013502700663217126 1105 30-31 -0.04059359306640431 1105 32-33 -0.13390573918140802 1105 34-35 -0.16978823004739496 1105 36-37 0.01128486263553441 1105 38-39 -0.008163806933630724 1105 40-41 0.11315611765702727 1105 42-43 0.1139453961282868 1105 44-45 0.11974975403093424 1105 46-47 0.20132419423023862 1105 48-49 0.1825769240206725 1105 50-51 0.15450028287698103 1105 52-53 0.186742221413013 1105 54-55 0.2421444306090237 1105 56-57 0.11115265740109237 1105 58-59 -0.04382549836452654 1105 60-61 0.13728846363002134 1105 62-63 0.2821180398578562 1105 64-65 0.12175777896272422 1105 66-67 0.20441444803111608 1105 68-69 0.17215935186856868 1105 70-71 -0.01772572025571506 1105 72-73 0.12328183211900878 1105 74-75 -0.015412871995824418 1105 76-77 0.020195800766114047 1105 78-79 0.10494282724749127 1105 80-81 0.43478250889207004 1105 82-83 0.4401228599138918 1105 84-85 0.6033402789503306 1105 86-87 0.30260138944764847 1105 88-89 0.19336096790378932 1105 90-91 0.22924904753115172 1105 92-93 0.4975118399611915 1105 94-95 0.5248555037784932 1105 96-97 0.3262041068562347 1105 98-99 0.41359281991125485 1105 100-101 0.720224867478894 1106 1 -0.04277995499445453 1106 2 0.049265346783993635 1106 3 -0.02939602270487285 1106 4 0.12969140060070572 1106 5 0.10151524676810197 1106 6 0.026769228806706735 1106 7 -0.22869207105129163 1106 8 -0.15683135621675603 1106 9 -0.2779434831737362 1106 10-11 -0.4553670324883399 1106 12-13 -0.25926516602518035 1106 14-15 -0.3415948404509024 1106 16-17 -0.022072646792864248 1106 18-19 -0.2566597003192257 1106 20-21 -0.16835739658259996 1106 22-23 -0.22765781646888428 1106 24-25 -0.27148240526380363 1106 26-27 -0.5135640425750907 1106 28-29 -0.24180381131726847 1106 30-31 -0.20630900510534644 1106 32-33 -0.12839246160930884 1106 34-35 0.12352099340888145 1106 36-37 -0.13165757074807516 1106 38-39 -0.07207783408295398 1106 40-41 0.03345354898387143 1106 42-43 -0.08402697463819919 1106 44-45 0.11899846283846927 1106 46-47 -0.14343847773831442 1106 48-49 0.16437168155071902 1106 50-51 -0.07111274788310595 1106 52-53 -0.08137732244183127 1106 54-55 -0.4111789616849606 1106 56-57 -0.3446839896120366 1106 58-59 -0.26343676944638617 1106 60-61 -0.20930530208138975 1106 62-63 -0.15235394240824007 1106 64-65 0.07288957200628232 1106 66-67 0.042575470015663086 1106 68-69 -0.28346590522727055 1106 70-71 -0.016280270280851994 1106 72-73 0.044433392976454655 1106 74-75 0.03641193718192781 1106 76-77 0.19112168860376855 1106 78-79 0.12525911226966002 1106 80-81 -0.27882128927055305 1106 82-83 -0.1250395103955384 1106 84-85 -0.42393759023930144 1106 86-87 -0.3091638877588032 1106 88-89 -0.09329518639942336 1106 90-91 0.303829695641209 1106 92-93 0.21332951901416308 1106 94-95 0.22687570579869387 1106 96-97 0.6267405801868264 1106 98-99 0.5746947898367551 1106 100-101 0.8575283319882416 1107 1 -0.08606466725334272 1107 2 -0.0500133077672551 1107 3 -0.05035489405829097 1107 4 -0.05774682672274167 1107 5 0.04738245544019293 1107 6 -0.09555262676479259 1107 7 -0.07041453239355633 1107 8 -0.0778659489156226 1107 9 0.02235541687408471 1107 10-11 -0.08179776738392519 1107 12-13 -0.10306597903331038 1107 14-15 0.07750287993805927 1107 16-17 -0.12655415484323385 1107 18-19 -0.16310398535031112 1107 20-21 -0.02458448091226728 1107 22-23 0.07653276220160876 1107 24-25 0.07349806659973268 1107 26-27 0.047240197820258345 1107 28-29 0.08102418166853909 1107 30-31 0.06889623959416014 1107 32-33 0.11985797677743193 1107 34-35 0.03784627636759552 1107 36-37 -0.10186919198193323 1107 38-39 -0.13777606459466796 1107 40-41 -0.18185212613170165 1107 42-43 -0.05945938337405465 1107 44-45 -0.0861725079888842 1107 46-47 -0.1159735486772604 1107 48-49 0.1207541939052561 1107 50-51 -0.16423206878219787 1107 52-53 -0.13071376433565973 1107 54-55 -0.09003477230189105 1107 56-57 -0.2144353047722376 1107 58-59 -0.1343318754562688 1107 60-61 0.2481911111180395 1107 62-63 0.1917935946553655 1107 64-65 0.2623702829898633 1107 66-67 0.02858296244353653 1107 68-69 -0.13186778630490537 1107 70-71 0.05455904105344089 1107 72-73 0.13678553708612995 1107 74-75 0.08167337509042127 1107 76-77 0.10262136977705083 1107 78-79 0.06144594472621989 1107 80-81 0.33048247288471444 1107 82-83 0.25934794377312187 1107 84-85 0.32221855514568176 1107 86-87 0.2842423771861995 1107 88-89 0.16639714581801357 1107 90-91 0.3693167034524656 1107 92-93 0.28702232947168227 1107 94-95 0.6645450824924595 1107 96-97 0.8974304414972032 1107 98-99 1.0642930681724785 1107 100-101 1.494155543848592 1108 1 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-0.22764322125130576 2316 12-13 0.14887703580968292 2316 14-15 0.19637361910417184 2316 16-17 0.09759740777330705 2316 18-19 0.0806716542409518 2316 20-21 0.1842952088020624 2316 22-23 0.18431600975885232 2316 24-25 0.1533369567317564 2316 26-27 0.48540309779787805 2316 28-29 0.3581182370127607 2316 30-31 0.441041387651147 2316 32-33 0.2523969489561395 2316 34-35 -0.06548407344966023 2316 36-37 0.075418791907083 2316 38-39 0.3535157978652421 2316 40-41 0.2338635447864732 2316 42-43 0.570677858365336 2316 44-45 0.5951879753407141 2316 46-47 0.6596548543957681 2316 48-49 0.5776796805913165 2316 50-51 0.06878426596830423 2316 52-53 0.18095178351248364 2316 54-55 0.2684868954835977 2316 56-57 0.6165410508628781 2316 58-59 0.45704203369567153 2316 60-61 0.5563584863697741 2316 62-63 0.4290838161814321 2316 64-65 0.3496157517748273 2316 66-67 0.5823682984620291 2316 68-69 0.6008763026038757 2316 70-71 0.7019559232791401 2316 72-73 0.5076626908117952 2316 74-75 0.7521626717265057 2316 76-77 0.870574677750497 2316 78-79 0.82979829797452 2316 80-81 0.9093368027124171 2316 82-83 0.5196485330175449 2316 84-85 0.5279804705629978 2316 86-87 0.7461540651104137 2316 88-89 0.7265075359127415 2316 90-91 0.7263727189667506 2316 92-93 0.5319764579120481 2316 94-95 0.938088753708989 2316 96-97 1.2115872252668787 2316 98-99 1.3732286988131897 2316 100-101 0.47207006611308344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4518.0 3 986.0 4 688.0 5 837.0 6 949.0 7 1183.0 8 1416.0 9 1875.0 10 2404.0 11 3469.0 12 4986.0 13 6914.0 14 7898.0 15 10150.0 16 13448.0 17 18882.0 18 15744.0 19 9451.0 20 8767.0 21 8830.0 22 9262.0 23 10186.0 24 11397.0 25 12814.0 26 14792.0 27 17121.0 28 20334.0 29 24109.0 30 29245.0 31 36226.0 32 45306.0 33 58633.0 34 78518.0 35 110975.0 36 156213.0 37 215925.0 38 175259.0 39 21145.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.43951631544972 19.214252324846708 14.920627310506374 20.425604049197197 2 14.725055379232188 21.068245702331918 41.44600567658529 22.760693241850607 3 19.005258365114035 27.735650756917007 30.368684655570238 22.890406222398717 4 11.631726750540725 17.02870485364265 38.61473425093231 32.72483414488432 5 12.175444722895525 39.097185133451106 33.730211714117466 14.9971584295359 6 28.766125248706388 37.1661512751232 18.88336398286366 15.18435949330676 7 26.800109585288173 34.97816855587138 21.01897195301531 17.202749905825144 8 26.633879921756957 33.78746827261793 20.224884540150924 19.353767265474186 9 27.597571017936595 16.537039922069873 20.91684314507638 34.94854591491715 10-11 24.020573237583996 27.874288546676944 27.184547471384562 20.920590744354495 12-13 26.132559887544453 25.562791390507368 28.16353995270977 20.141108769238407 14-15 23.0293710687248 25.7956278292235 28.335996089963366 22.839005012088332 16-17 20.23859869601545 29.626611146876325 28.563528154834465 21.571262002273755 18-19 20.15820528338194 27.21174529993691 32.72705950631254 19.9029899103686 20-21 21.665846382976902 26.032808494429915 32.50266413876632 19.79868098382686 22-23 20.955097534044903 26.283049875461135 32.32870556615966 20.433147024334293 24-25 20.436146643802875 26.451329353709895 32.906184992149306 20.20633901033792 26-27 20.532351937932074 26.475848659790106 32.77119086922227 20.220608533055543 28-29 20.100612208122513 26.505833804520574 33.20326996096814 20.19028402638877 30-31 20.20670564904983 26.739943045942056 33.01764480032051 20.035706504687603 32-33 19.909129626181628 26.865014906079924 32.69390209712387 20.531953370614584 34-35 20.35857474181129 26.545429484067768 32.66614784926214 20.4298479248588 36-37 20.564348219448114 26.51216058156121 32.48423889853964 20.439252300451038 38-39 20.5964786294207 26.573033924251288 32.62063118648217 20.209856259845836 40-41 20.357187028047637 26.72800094834474 32.609150585247676 20.305661438359945 42-43 20.157862391037888 27.072548395269692 32.15965816882023 20.60993104487219 44-45 20.26488382386052 27.34122251833538 31.702718181794832 20.691175476009267 46-47 20.543234123102646 27.369618288391294 30.920990392892715 21.16615719561334 48-49 20.681242604867474 27.71451663444629 30.567689328235154 21.036551432451084 50-51 21.09159418453427 27.92320818089896 30.228195642253482 20.757001992313285 52-53 20.749277811483 28.21384058718732 30.168491387042952 20.868390214286723 54-55 20.444074962264587 28.252159030685764 30.20733715125244 21.09642885579721 56-57 20.79098685483139 28.570499290445063 29.703305036727386 20.935208817996163 58-59 20.835781298473457 27.848393756886576 30.085217000609767 21.23060794403021 60-61 20.76207510354979 27.876758634854347 30.416809297230685 20.94435696436518 62-63 20.985428634026444 27.89565888659907 30.449199627542672 20.66971285183182 64-65 20.797252917705205 27.901028016388356 30.598089451332356 20.703629614574083 66-67 20.647187568863966 28.149257499352814 30.224151542445878 20.979403389337346 68-69 20.49709640549935 28.491127893806024 30.084585295843137 20.927190404851494 70-71 20.534057254408932 28.327320389421384 29.87124412095838 21.267378235211304 72-73 20.537824072766547 28.27894071542703 29.690855142158533 21.492380069647883 74-75 20.508038667767874 28.471604695249546 29.69274615611748 21.327610480865104 76-77 20.66025013598999 28.14605517748459 29.943947728485654 21.249746958039758 78-79 20.469470508307744 28.227117592305305 29.87184781794644 21.431564081440506 80-81 20.341176405139173 28.759237537535356 29.65572105749879 21.243864999826688 82-83 20.427882360593333 28.629845299592965 29.46928928188749 21.472983057926214 84-85 20.409024347869252 28.19355567556372 29.460074509666317 21.937345466900712 86-87 20.583512785311527 28.18331832669732 29.602386911018066 21.630781976973086 88-89 20.789297218048684 27.73396929921222 29.702804631778406 21.77392885096069 90-91 20.401559365759773 27.90706632012192 29.920127228085704 21.7712470860326 92-93 20.31832052608088 28.08701713905223 29.740969271580653 21.853693063286244 94-95 20.16117126458079 27.973467334619084 29.740428851470735 22.124932549329387 96-97 20.196074571332954 27.827730326422905 29.47790480608663 22.49829029615751 98-99 20.277702167143442 27.765252091561337 29.583973170493707 22.373072570801515 100-101 20.393151092312202 27.569399426191104 30.062023564247554 21.97542591724914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2325.0 1 1492.0 2 871.0 3 1109.0 4 916.5 5 786.0 6 1265.0 7 2549.0 8 3962.0 9 4446.0 10 3830.5 11 3184.5 12 3137.0 13 3314.5 14 3512.5 15 3561.5 16 3665.0 17 3874.5 18 3996.0 19 4101.5 20 4417.0 21 4840.5 22 5313.5 23 6083.5 24 6990.0 25 8084.0 26 9676.0 27 11617.5 28 13213.5 29 15221.0 30 17717.5 31 19337.0 32 21136.5 33 23695.5 34 26566.5 35 28913.5 36 31686.0 37 34666.5 38 36060.5 39 36212.0 40 36664.5 41 37786.5 42 38169.0 43 39178.0 44 41272.5 45 42434.5 46 42238.0 47 43444.0 48 50003.5 49 49048.5 50 41263.0 51 37075.5 52 33058.0 53 30471.0 54 28735.0 55 26494.5 56 23299.0 57 20098.5 58 17987.5 59 16449.0 60 14178.5 61 11644.5 62 9264.5 63 6731.0 64 4732.0 65 3499.5 66 2543.0 67 1872.5 68 1364.5 69 940.5 70 661.5 71 495.5 72 409.5 73 342.0 74 269.5 75 198.5 76 132.5 77 71.5 78 44.0 79 34.0 80 21.5 81 14.0 82 11.0 83 5.5 84 4.5 85 4.5 86 1.5 87 1.5 88 2.5 89 1.5 90 1.0 91 2.5 92 2.0 93 1.0 94 1.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2415314528953882 2 0.2181298906275889 3 0.2082226598864768 4 0.21548226861918826 5 0.21300546093391023 6 0.24067738127977506 7 0.24101900992602032 8 0.23119718634646952 9 0.2251332778756164 10-11 0.23422914058189606 12-13 0.23538213726297377 14-15 0.22090562337833153 16-17 0.2348696942936059 18-19 0.22487705639093247 20-21 0.21924018372788595 22-23 0.2179590763044663 24-25 0.21296275735312956 26-27 0.2215034735092607 28-29 0.23175233289661804 30-31 0.2329480331584764 32-33 0.23183774005817934 34-35 0.240933602764459 36-37 0.21851422285461478 38-39 0.2168060796233886 40-41 0.21441467909967185 42-43 0.21915477656632462 44-45 0.23615080171702557 46-47 0.24089089918367837 48-49 0.24460611071159538 50-51 0.24537477516564718 52-53 0.2611323964737091 54-55 0.24434988922691145 56-57 0.21556767578074956 58-59 0.20279930512733355 60-61 0.2000235723765909 62-63 0.20548963071651485 64-65 0.20066412608830075 66-67 0.20023709028049422 68-69 0.20467826268168238 70-71 0.20587396294354074 72-73 0.206087480847444 74-75 0.2089913243405286 76-77 0.22013695892427973 78-79 0.21561037936153024 80-81 0.21040054250629023 82-83 0.20583125936276006 84-85 0.20694155246305715 86-87 0.212663832287665 88-89 0.20971728521379973 90-91 0.2191974801471053 92-93 0.20911943508287056 94-95 0.2063009987513473 96-97 0.21616552591167876 98-99 0.22171699141316398 100-101 0.20109116189610732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1170862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.34218972334285 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9448014029754 44.47922978318062 2 12.09198880562148 13.625782548377266 3 3.7415956606348644 6.324290777385776 4 1.646261587066294 3.7101593069096257 5 0.8785055937899572 2.474846441916587 6 0.5583448455860399 1.8875022732635538 7 0.38323777584926455 1.5114718833235845 8 0.2623276133230745 1.182408972761631 9 0.18991609454611286 0.9630259767390067 >10 1.1725141393059861 12.263523058665426 >50 0.09096743201842701 3.5182102541897917 >100 0.03544880689063164 3.54275110367911 >500 0.0019693759667387665 0.7808949795308611 >1k 0.0016663950487789563 1.5730565605808033 >5k 3.029809179598102E-4 1.2212313222349502 >10k+ 1.514904589799051E-4 0.9416147572613902 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11032 0.9422118063443856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8396 0.7170785284687692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5912 0.504927139150472 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2819 0.2407627884413364 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2499 0.2134324967417168 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1985 0.1695332156992028 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1859 0.1587719133424776 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1721 0.14698572504701665 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1661 0.14186129535333797 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.13690767998278192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.540716156131124E-5 0.0 22-23 0.0 0.0 0.0 4.6973938858721183E-4 0.0 24-25 0.0 0.0 0.0 8.113680348324568E-4 0.0 26-27 0.0 0.0 0.0 0.0011957002618583573 0.0 28-29 0.0 0.0 0.0 0.0027330291699619595 0.0 30-31 0.0 4.270358078065562E-5 0.0 0.006277426374756376 0.0 32-33 0.0 8.540716156131124E-5 0.0 0.009352084190963582 0.0 34-35 0.0 8.540716156131124E-5 0.0 0.011914299037802917 0.0 36-37 0.0 8.540716156131124E-5 0.0 0.017423060958507493 0.0 38-39 0.0 8.540716156131124E-5 0.0 0.02489618759512223 0.0 40-41 0.0 8.540716156131124E-5 0.0 0.03369312523593729 0.0 42-43 0.0 8.540716156131124E-5 0.0 0.043258727330804145 0.0 44-45 0.0 8.540716156131124E-5 0.0 0.04718745676262446 0.0 46-47 0.0 8.540716156131124E-5 0.0 0.053294068814258216 0.0 48-49 0.0 8.540716156131124E-5 0.0 0.06486673920581588 0.0 50-51 0.0 8.540716156131124E-5 0.0 0.07797673850547716 0.0 52-53 0.0 8.540716156131124E-5 0.0 0.10470918007416757 0.0 54-55 0.0 8.540716156131124E-5 0.0 0.12520689884888225 0.0 56-57 0.0 8.540716156131124E-5 0.0 0.17487116329678476 0.0 58-59 0.0 8.540716156131124E-5 0.0 0.26061995350434125 0.0 60-61 0.0 8.540716156131124E-5 0.0 0.3329598193467719 0.0 62-63 0.0 8.540716156131124E-5 0.0 0.37378444257307863 0.0 64-65 0.0 8.540716156131124E-5 0.0 0.45299958492119485 0.0 66-67 0.0 8.540716156131124E-5 0.0 0.5861920533760596 0.0 68-69 0.0 8.540716156131124E-5 0.0 0.7721234440950342 0.0 70-71 0.0 8.540716156131124E-5 0.0 0.9555780271287307 0.0 72-73 0.0 8.540716156131124E-5 0.0 1.162989318980375 0.0 74-75 0.0 8.540716156131124E-5 0.0 1.395382205588703 0.0 76-77 0.0 8.540716156131124E-5 0.0 1.5725593622476433 0.0 78-79 0.0 8.540716156131124E-5 0.0 1.8016213695550798 0.0 80-81 0.0 8.540716156131124E-5 0.0 2.1090871511758005 0.0 82-83 0.0 8.540716156131124E-5 0.0 2.40540729821277 0.0 84-85 0.0 8.540716156131124E-5 0.0 2.763220601573883 0.0 86-87 0.0 8.540716156131124E-5 0.0 3.210113574443444 0.0 88-89 0.0 8.540716156131124E-5 0.0 3.7632103527144958 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12825 0.0 28.7147 1 TACCTGG 1260 0.0 27.901539 2 GTACATG 10295 0.0 27.001598 1 TACATGG 10270 0.0 26.783981 2 GGTATCA 8900 0.0 26.42866 1 CATGGGG 2840 0.0 25.929787 4 ACATGGG 10305 0.0 25.632668 3 TATCAAC 15350 0.0 23.986137 2 ATCAACG 15450 0.0 23.738638 3 TCAACGC 15600 0.0 23.511389 4 CAACGCA 15725 0.0 23.293283 5 GAGTACT 7715 0.0 23.150648 12-13 ATGGGAT 2530 0.0 23.096786 5 AACGCAG 16030 0.0 22.965687 6 CATGGGA 6970 0.0 22.834822 4 GTACACG 230 3.610694E-9 22.726095 1 GTACCTG 1590 0.0 22.414244 1 AGTACTT 8030 0.0 21.650938 12-13 GGGCGCG 155 3.90527E-5 21.452482 7 GTACTTT 8460 0.0 21.140041 14-15 >>END_MODULE