FastQCFastQC Report
Thu 26 May 2016
SRR937388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170862
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128671.0989339478093918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102630.8765336991037372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74260.6342335817542972No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32100.2741569886118091No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30520.26066265708512193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.20958917447145778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22840.19506995700603488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.17226624486916475No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.1321248789353485No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15140.12930644260382523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.1026594081966961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12850.033.637742
CTCGACT700.00839211127.142544
GTACCTG16100.026.5695061
GTCGGGA2700.026.388582
GGTATCA112450.025.2337441
CGTACAC1351.3428777E-524.6293433
GTATCAA152350.024.5838891
ACCTGGG17700.024.152263
GTACATG105050.023.979861
ACGTGCC1401.7790908E-523.7507388
GAGTACT91350.023.42574112-13
TACATGG106800.023.304972
ACATGGG106850.022.2271633
GTACTTT97700.021.94993214-15
CGGGAGT3500.021.7140334
AGTACTT94000.021.7041312-13
TATCAAC183350.020.3883632
CATGGGG26800.020.3822254
TATAACG1404.852194E-420.3569052
ATCAACG183400.020.3051073
Produced by FastQC (version 0.11.5)